Human Cytokine 16-Plex ELISA Kit (Serum, Plasma, TCM)

Product Features: 
  • Cytokine Multiplex ELISA kit with unique and customizable array of molecules including Type I, II & III IFNs, ILs, TNF Alpha and more
  • Simultaneous quantification of up to 16 pro-inflammatory and anti-inflammatory cytokines in a single 50 μl sample
  • Provides analyte sensitivity as low as 5 pg/ml
  • Reproducible results with excellent intra- and inter-assay CVs (< 15%) for most analytes in difficult matrices, including autoimmune serum and plasma

Full Product Name: VeriPlex Human Cytokine 16-Plex ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
51510-1

1 x 96-well plate

$1,675.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media and Buffers
 
Assay Range: 2.86 log with LLOD and LLOQ in the low pg/ml range
 
Speed: Incubation time, 3 hours, 15 minutes
 
Specificity: Human IFN Alpha (IFN-α), IFN Beta (IFN-β), IFN Gamma (IFN-γ) and IFN Lambda (IFN-λ 1, 2 and 3); Human Interleukin-1 Alpha (IL-1α), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-10 (IL-10), Interleukin-12 (IL-12p70), Interleukin-13 (IL-13), Interleukin-15 (IL-15), Interleukin-17 (IL-17) and Interleukin-23 (IL-23); and Human Tumor Necrosis Factor-Alpha (TNF-α).
 
Inter- and Intra-Assay CV: > 15% for most analytes in difficult matrices, including autoimmune serum and plasma
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Human Cytokine 16-Plex microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Cytokine Antigen Standard
  • Sample Diluent
  • Assay Diluent
  • Human Cytokine Detection Mix
  • HRP Concentrate
  • Substrate A
  • Substrate B
  • Diluent Additive II
  • Diluent Additive III

Tech Info / Data

51510-1 VeriPlex Human Cytokine 16-Plex ELISA Analytes

Detailed related product information:

Background: 

VeriPlex Human Cytokine 16-Plex ELISA Kit is a chemiluminiscence based sandwich enzyme linked immunosorbent assay (ELISA) that can simultaneously quantitate Type I, II & III IFNs (IFN-α; IFN-β; IFN-γ; IFN-λ1/2/3) and 12 other key innate and adaptive immunity cytokines (TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, and IL-23) in low volumes of serum, plasma and cell culture supernatants. The kit employs a cost-effective multiplex ELISA technology in which 16 distinct capture antibodies are used in each well of a 96-well plate in a 4x4 array, and provides unique chemiluminescent results on each analyte within a sample in various matrices. The assay is compatible with multiple matrices including tissue culture media, human serum, human plasma and buffers. 

This product has been developed to work in samples that are known to measure false positives in immunoassays such as samples containing human anti-mouse antibodies, anti-nuclease antibodies, and rheumatoid factor. The use of this product has been evaluated in serum from Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) patients.

  • Sample volume required: 50 μl to quantify all 16 analytes
  • Compatible with serum and plasma from auto-immune disease patients
  • Detects 14 subtypes and alleles of IFN-α at different sensitivities
  • Detects all 3 subtypes of IFN-λ

Note: The product requires a Gel-DOC camera to measure chemiluminiscence and image analysis software to perform subsequent image analysis. We recommend using the Quansys Q-ViewTM imager in conjunction with the latest version of Q-ViewTM software for optimal image analysis. This software is available free of charge, and can be downloaded here.

Citations

Representative analyte standard curves for PBL's Human Cytokine 16-Plex ELISA kit (51510) quantifying Human IFN alpha, IL-6, TNF alpha, IFN gamma, IFN beta, IFN lambda, IL-1a, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23

Image of representative analyte standard curves for PBL's Human Cytokine 16-Plex ELISA kit, a multiplex quantifying Human Interferon alpha, IL-6, TNF alpha, Interferon gamma, Interferon beta, Interferon lambda, IL-1a, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23

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