Human IFN 9-Plex ELISA Kit (Serum, Plasma, TCM)

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Product Features: 
  • Human IFN Multiplex ELISA simultaneously quantifies type I, II, III Human IFNs and related pro-inflammatory cytokines (IFN-alpha, IFN-beta, IFN-gamma, IFN-lambda, IFN-omega, IL-1alpha, IL-6, IP-10 and TNF-alpha)
  • Sample volume of only 50 μl required to quantify all 9 analytes in a single assay
  • Compatible with auotimmune sera, plasma, tissue culture media (TCM) and buffers

Full Product Name: VeriPlex Human Interferon 9-Plex ELISA Kit

 

Note: The product requires a Gel-DOC camera to measure chemiluminiscence and image analysis software to perform subsequent image analysis. We recommend using the Quansys Q-ViewTM imager in conjunction with the latest version of Q-ViewTM software for optimal image analysis. This software is available for download here.

Ordering

Catalog No. Pack Size Price Quantity
51500-1

Available for assay service

 $0.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media and Buffers
 
Assay Range: 2.86 log with LLOD and LLOQ in the low pg/ml range
 
Speed: Incubation time, 3 hours, 15 minutes
 
Specificity: Human IFN Alpha (IFN-α), IFN Beta (IFN-β), IFN Gamma (IFN-γ), IFN Omega (IFN-ω) and IFN Lambda (IFN-λ 1, 2 and 3); Human Interleukin 1 Alpha (IL-1α), Human Interleukin-6 (IL-6), Human IFN Gamma inducing protein-10 (IP-10) and Human Tumor Necrosis Factor-Alpha (TNF-α)
  • No cross-reactivity against mouse IFN-β, IFN-γ, and IFN-λ3.
  • Minimal cross-reactivity against mouse IFN-α (0.004%).
  • Low cross-reactivity against Rhesus monkey IFN-α (11%) and Cynomolgus monkey IFN-α (6%). Detects Cynomolgus monkey IFN-β.
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Human Interferon Multiplex 96-well microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Multiplex Antigen Standard
  • Sample Diluent
  • Assay Diluent
  • Human Interferon Multiplex Detection Mix
  • HRP Concentrate
  • Substrate A
  • Substrate B
  • Diluent Additive II
  • Diluent Additive III

Tech Info / Data

Background: 

VeriPlex Human IFN Multiplex ELISA is a 9-Plex kit developed to simultaneously quantitate 9 human cytokines, including Interferon Alpha (IFN-α), Interferon Beta (IFN-β), Interferon Gamma (IFN-γ), Interferon Omega (IFN-ω), Interferon Lambda (IFN-λ1/2/3), Interleukin-1alpha (IL-1α), Interleukin-6 (IL-6), Interferon Gamma inducing protein-10 (IP-10), and Tumor Necrosis Factor-Alpha (TNF-α), in low volumes of serum, plasma and cell culture supernatants. This assay has been developed using the Q-PlexTM array spotting technology, in which capture antibodies to the different analytes are spotted in a single well in a 3x3 array. The functional format of the assay is as that of sandwich ELISA with a chemiluminiscent output. The assay is compatible with multiple matrices including tissue culture media, human serum, human plasma and buffers.

 

This kit can be used with samples that are known to measure false positives in immunoassays such as samples containing human anti-mouse antibodies and rheumatoid factor. The use of this product has been evaluated in serum from Rheumatoid Arthritis patients.

 

 

Citations

3 Citations:

  1. Aiello, A. et al., (2021), HIV-1 Nef Protein Affects Cytokine and Extracellular Vesicles Production in the GEN2.2 Plasmacytoid Dendritic Cell Line, Viruses, 14:74, DOI: 10.3390/v14010074 (link)
  2. Mitchell, Angela, et al. (2015). Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver. mBio, 11 pgs. PMID: 25714713. (link)

  3. Banos-Lara, M. Del Rocia, et al. (2013). Critical Role of MDA5 in the Interferon Response Induced by Human Metapneumovirus Infection in Dendritic Cells and In Vivo. JVI, 10 pgs. PMID: 23152520. (link)  

References: (For additional references, please refer to the protocol.)

  1. Evolution of the class 2 cytokines and receptors, and discovery of new friends and relatives; Pestka S et al; Pharmacology & Therapeutics; 2005 June; 106(3); 299-346.

  2. Interferon-g: an overview of signals, mechanism, and functions; Hume D et al; Journal of Leukocyte Biology; 2004; 75; 163-189.

  3. IFN-λ: Novel anti-viral cytokines; Paludan S et al; Journal of Interferon & Cytokine Research; 2006 May; 26 (6); 373-79.

  4. Interferon Lambda as a potential new therapeutic for hepatits C; Williams D et al; Annals of the New York Academy of Sciences; 2009 Dec; 1182; 80-87.

Documentation

Human IFN 9-Plex ELISA Analytes

51500-1 VeriPlex Human Interferon 9-Plex ELISA Analytes

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