Human IFN Gamma ELISA Kit (TCM)

Product Features: 
  • Quantifies IFN Gamma in tissue culture media (TCM)

Full Product Name: VeriKine Human Interferon Gamma ELISA Kit

 

NEW Human IFN Gamma ELISA coming soon... 

Ordering

Catalog No. Pack Size Price Quantity
41500-1

1 x 96-well plate

$350.00

Specifications

Compatibility: Tissue Culture Media
Assay Range: 12.5 - 500 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Gamma (IFN-gamma, IFNG)
No cross-reactivity against human IFN-α or IFN-β; mouse or rat IFN-α, IFN-β or IFN-γ.
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Gamma Standard, 10,000 pg/ml
  • Dilution Buffer 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Assay Diluent
  • TMB Substrate Solution
  • Stop Solution 

Tech Info / Data

Background: 

Human IFN Gamma ELISA kit for quantitative measurement of human interferon gamma (IFNG-γ) in tissue culture media. This kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. All reagents are supplied. One pre-coated microtiter plate (96-well) is included. The assay is based on the international reference standard for human interferon gamma (Hu-IFN-γ) provided by the National Institutes of Health. Typical standard curves for each lot are included with the procedure.


Interferon Gamma (IFN-gamma or IFNG) is a pleiotropic cytokine secreted by Natural Killer (NK) cells and many T cells. It is the sole representative of Type II IFN. IFN-gamma has pleiotropic activities including: inducing an antiviral state in cells, upregulating cell surface markers such as Class I and II MHC, and regulating the activity of macrophages, NK cells, neutrophilis, B cells and T cells.

Citations

Typical IFN-gamma Standard Curve from 12.5 to 500 pg/ml using PBL's Human IFN-γ ELISA for TCM (41500)

Typical IFN-γ Standard Curve from 12.5 to 500 pg/ml using PBL's Human IFN-γ ELISA for TCM (41500)

Assay Range: 12.5 - 500 pg/ml

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