Mouse Cytokine 9-Plex ELISA Kit (Serum, Plasma, TCM)

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Product Features: 
  • Simultaneously profiles each mouse sample for pro-inflammatory (IFN-alpha, IFN-beta, IFN-gamma, IL-1 alpha, IL-1 beta, IL-6 and TNF-alpha) and inhibitory cytokines (IL-10), as well as chemokines (RANTES)
  • Small sample volume of 50 μl needed to quantify all 9 analytes in a single assay
  • Compatible with mouse serum, plasma, tissue culture media (TCM) and buffers
  • Broad quantitative range reduces the need to dilute samples

Full Product Name: VeriPlex Mouse Cytokine 9-Plex ELISA Kit

 

Note: The product requires a Gel-DOC camera to measure chemiluminiscence and image analysis software to perform subsequent image analysis. We recommend using the Quansys Q-ViewTM imager in conjunction with the latest version of Q-ViewTM software for optimal image analysis. This software is available for download here.

Ordering

Catalog No. Pack Size Price Quantity
52500-1

Available for assay service

 $0.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media and Buffers
 
Assay Range: 2.86 log with LLOD and LLOQ in the low pg/ml range
 
Speed: Incubation time, 3 hours, 20 minutes
 
Specificity: Mouse Interferon Alpha (IFN-α), Interferon Beta (IFN-β), Interferon Gamma (IFN-γ); Mouse Interleukin 1 Alpha (IL-1α), Interleukin 1 Beta (IL-1β), Interleukin-6 (IL-6), Interleukin-10 (IL-10); mouse chemokine RANTES (CCL5); and Mouse Tumor Necrosis Factor-Alpha (TNF-α)
  • No cross-reactivity against Human Interferon Alpha 2a, Interferon Beta 1a, Interferon Gamma, Interferon Lambda 1, 2 or 3; Rat Interferon Alpha, Interferon Beta
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Cytokine 9-Plex Antigen Standard
  • Mouse Cytokine 9-Plex Detection Mix
  • HRP Concentrate
  • Mouse Cytokine 9-Plex Sample Diluent
  • Substrate A
  • Substrate B+

Tech Info / Data

Background: 

VeriPlex Mouse Cytokine 9-Plex ELISA is a multiplex kit that has been developed to simultaneously quantitate Type I and II IFNs (IFN-α, IFN-β, IFN-γ), and other key cytokines released upstream and downstream of interferon signaling, including IL-1α, IL- 1β, IL-6, IL-10, RANTES and TNF-α.

 

This multiplex assay is a chemiluminescent based sandwich enzyme-linked immunosorbent assay (ELISA) that employs cost-effective multiplex ELISA technology. This assay has been developed using the Q-PlexTM array spotting technology, in which capture antibodies to the different analytes are coated in a 3x3 array in each well of a 96-well plate. The assay is compatible with multiple matrices including tissue culture media, mouse serum, mouse plasma and buffers.

 

Note: The product requires a Gel-DOC camera to measure chemiluminiscence and image analysis software to perform subsequent image analysis. We recommend using the Quansys Q-ViewTM imager in conjunction with the latest version of Q-ViewTM software for optimal image analysis. This software is available for download here.

Citations

Citations:

  1. Aw et al., (2020), Microglial responses to peripheral type I interferon, J. Neuroinflammation, PMID: 33183319 (link)

 

References: (For additional references, please refer to the protocol.)

  1. Evolution of the class 2 cytokines and receptors, and discovery of new friends and relatives; Pestka S et al; Pharmacology & Therapeutics; 2005 June; 106(3); 299-346.

  2. Interferons, Interferon-like cytokines, and their receptors; Pestka S et al; Immunological Reviews; 2004; 202; 8-32.

  3. Interferon Alpha in malignant and viral diseases: a review; Dorr RT; Drugs; 1993; 45 (2).

  4. Interferon-treatment for relapsing multiple sclerosis; Zivadinov R et al; Expert Opinion in Biological Therapeutics; 2008; 8 (9); 1435-37.

  5. Interferon-γ: an overview of signals, mechanism, and functions; Hume DA et al; Journal of Leukocyte Biology; 2004; 75; 163-89.

  6. Microglial responses to peripheral type 1 interferon; Aw et al; Journal of Neuroinflammation; 2020.

Documentation

Representative analyte standard curves for PBL's Mouse Cytokine 9-Plex ELISA kit (52500) quantifying Mouse IFN alpha, IFN beta, IFN gamma, IL-1a, IL-1b, IL-6, IL-10, RANTES and TNF alpha

52500 Mouse Multiplex ELISA Analyte Standard Curves

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