Proliferation & Anti-Proliferation Assays

Service Features:

  • Evaluate cytokine and other modulator activities with growth inhibition, growth promotion, and cytotoxicity assays
  • Utilizes cell lines sensitive to IFNs or other growth-modulating cytokines
  • Allows cell line screening to address specific client applications and clinical indications

Figure 1: OVCAR-3 human adenocarcinoma cell line grown in the presence of IFN-alpha. Cellular antiproliferation was quantified using soluble tetrazolium reagent. The quantity of reduced reagent product is directly proportional to viable cell number. EC50 was determined by nonlinear regression using a four-parameter (variable slope) curve fit.

PBL Growth Inhibition Assay Service

Figure 2. TF-1 human erythroleukemia cell line grown in the presence of GM-CSF. Cellular proliferation was quantified using soluble tetrazolium reagent. The quantity of reduced reagent product is directly proportional to viable cell number. EC50 was determined by nonlinear regression using a four-parameter (variable slope) curve fit.

PBL Growth Promotion Assay Service

Service Citations:

  1. High yield soluble bacterial expression and streamlined purification of recombinant human interferon α-2a. Bis et al., 2014, Protein Expression and Purification, 11:138-146. (link)

Interested in our services?

Please include any project requirements or preferences including, for example, (1) number of samples, (2) sample matrix type(s), (3) volume of each sample, and (4) preferred number of replicates (e.g. singlet, duplicate, triplicate).

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