Proliferation & Anti-Proliferation Assays

Service Features:

  • Evaluate cytokine and other modulator activities with growth inhibition, growth promotion, and cytotoxicity assays
  • Utilizes cell lines sensitive to IFNs or other growth-modulating cytokines
  • Allows cell line screening to address specific client applications and clinical indications

Figure 1: OVCAR-3 human adenocarcinoma cell line grown in the presence of IFN-alpha. Cellular antiproliferation was quantified using soluble tetrazolium reagent. The quantity of reduced reagent product is directly proportional to viable cell number. EC50 was determined by nonlinear regression using a four-parameter (variable slope) curve fit.

PBL Growth Inhibition Assay Service

Figure 2. TF-1 human erythroleukemia cell line grown in the presence of GM-CSF. Cellular proliferation was quantified using soluble tetrazolium reagent. The quantity of reduced reagent product is directly proportional to viable cell number. EC50 was determined by nonlinear regression using a four-parameter (variable slope) curve fit.

PBL Growth Promotion Assay Service

Service Citations:

  1. High yield soluble bacterial expression and streamlined purification of recombinant human interferon α-2a. Bis et al., 2014, Protein Expression and Purification, 11:138-146. (link)

Interested in our services?

Find your lot-specific Certificate of Analysis here!

Finding the right biomarker detection service to meet your project needs just got easier...

Sign up for our email list to be kept up-to-date on the latest product releases and company news!

Go to top