Proliferation & Anti-Proliferation Assays

Service Features:

  • Growth inhibition, growth promotion, and cytotoxicity assay services to evaluate cytokine and other modulator activities
  • Utilizes cell lines sensitive to IFNs or other growth-modulating cytokines
  • Allows cell line screening to address specific client applications and clinical indications

Figure 1: OVCAR-3 human adenocarcinoma cell line grown in the presence of IFN-alpha. Cellular antiproliferation was quantified using soluble tetrazolium reagent. The quantity of reduced reagent product is directly proportional to viable cell number. EC50 was determined by nonlinear regression using a four-parameter (variable slope) curve fit.

PBL Growth Inhibition Assay Service

Figure 2. TF-1 human erythroleukemia cell line grown in the presence of GM-CSF. Cellular proliferation was quantified using soluble tetrazolium reagent. The quantity of reduced reagent product is directly proportional to viable cell number. EC50 was determined by nonlinear regression using a four-parameter (variable slope) curve fit.

PBL Growth Promotion Assay Service

Service Citations:

  1. High yield soluble bacterial expression and streamlined purification of recombinant human interferon α-2a. Bis et al., 2014, Protein Expression and Purification, 11:138-146. (link)

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