Reagent Development Services: Proteins, Cytokines & Antibodies

PBL's reagent development services offer customized production of cytokines, interferons, antibodies, and other proteins.

 

Selected capabilities:

 

Protein Biophysical Characterization Service

 

With a wealth of knowledge on immunological interactions, we offer protein biophysical characterization services which can determine the kinetic characterization of protein-protein interactions. These services include determination of:

  • Affinity of protein-protein interactions
  • Association and dissociation rates of protein-protein interactions
  • Antibody-antigen specificity and affinity

PBL offers biophysical characterization of antibody affinity for a ligand or the measurement of antigen:antibody Kon/Koff rates. To accelerate assay development, we also determine which antibodies bind to antigens in a sandwich format. Binning— determination of antibodies which bind to non-overlapping epitopes—is performed using multiple hybridoma clones.

 

The graph below shows the association and dissociation of IFN to a surface-immobilized antibody using varying concentrations of IFN. The raw data is shown in blue and the model response in red.

 

Figure 1. IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by SPR

IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by SPR

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