Reagent Development Services: Proteins, Cytokines & Antibodies
PBL's reagent development services offer customized production of cytokines, interferons, antibodies, and other proteins.
Select capabilities:
- Recombinant protein production and purification (bacterial & mammalian)
- Cytokine and antibody production and characterization
- Hybridoma, polyclonal antibody, and cell line development
- Protein and antibody labeling and biophysical characterization
Protein Biophysical Characterization Service
With a wealth of knowledge on immunological interactions, we offer protein biophysical characterization services which can determine the kinetic characterization of protein-protein interactions. These services include determination of:
- Affinity of protein-protein interactions
- Association and dissociation rates of protein-protein interactions
- Antibody-antigen specificity and affinity
PBL offers biophysical characterization of antibody affinity for a ligand or the measurement of antigen:antibody Kon/Koff rates. To accelerate assay development, we also determine which antibodies bind to antigens in a sandwich format. Binning— determination of antibodies which bind to non-overlapping epitopes—is performed using multiple hybridoma clones.
The graph below shows the association and dissociation of IFN to a surface-immobilized antibody as determined by real time Biolayer Interferometry (BLI) using varying concentrations of IFN. The raw data is shown in blue and the model response in red.
Figure 1. IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by Bio-Layer Interferometry (BLI)
Protein & Antibody Labeling Service
PBL offers custom antibody labeling using a range of dyes and labels including FITC, R-PE, biotin, etc. For a comprehensive list of PBL-labeled monoclonal antibodies, click here.
The graph below shows an example of a R-Phycoerythrin (PE) Conjugation of a Mouse Monoclonal Antibody.
Figure 2. Flow cytometry analysis of KG-1 cells showed expression of Human Interferon Alpha/Beta Receptor. Isotype control (shaded histogram) and human interferon alpha/beta receptor (un-shaded histogram). (Catalog No. 21370-3)
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