Reagent Development Services: Proteins, Cytokines & Antibodies

Selected capabilities:

• Recombinant protein production and purification (bacterial & mammalian)
• Cytokine and antibody production and characterization
• Hybridoma, polyclonal antibody, and cell line development
• Protein and antibody labeling and biophysical characterization

Protein Biophysical Characterization Service

With a wealth of knowledge on immunological interactions, we offer protein biophysical characterization services which can determine the kinetic characterization of protein-protein interactions. These services include determination of:

• Affinity of protein-protein interactions
• Association and dissociation rates of protein-protein interactions
• Antibody-antigen specificity and affinity

PBL offers biophysical characterization of antibody affinity for a ligand or the measurement of antigen:antibody K_on/K_off rates. To accelerate assay development, we also determine which antibodies bind to antigens in a sandwich format. Binning— determination of antibodies which bind to non-overlapping epitopes—is performed using multiple hybridoma clones.

The graph to the right shows the association and dissociation of IFN to a surface-immobilized antibody using varying concentrations of IFN. The raw data is shown in blue and the model response in red.

Interested in one of our services?

Contact us:

W: online form

E: assayservices@pblassaysci.com

P: 1-877-PBL-8881

F: 1-732-777-9141

Figure 1. IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by SPR