Simoa Assay Services: Ultra-Sensitive Biomarker Testing

Advantages of Simoa Single Molecule Array Technology:

  • Delivers femtogram per milliliter (fg/ml) level sensitivity for measurement of low-abundance biomarkers in serum, plasma and other complex matrices including bronchial lavage, urine and sputum

  • Provides robust quantitative detection of various single analytes. Comprehensive multiplex panel options also available

  • Offers a high degree of precision, accuracy, and reproducibility with < 10% inter- and intra-assay CVs

  • Over 50 analytes to choose from including IL-17A, TNF-alpha, IL-23, IP-10, TRAIL, IL-6, IL-10 and IL-12p70

For an in-depth overview of the ultra-sensitive biomarker detection technology (Simoa), click here.

 

For additional information, please see the Ultra-Sensitive Biomarker Services Flyer

 

Figure 1. Normal Human Serum (NHS) Endogenous Levels of Several Pro- and Anti-Inflammatory Cytokines

PBL Quanterix Simoa Multiplex shows Normal Human Serum (NHS) Endogenous Levels for IL-1 Beta, IL-6, IL-10 and TNF-Alpha

Interested in our services?

Please include any project requirements or preferences including, for example, (1) number of samples, (2) sample matrix type(s), (3) volume of each sample, and (4) preferred number of replicates (e.g. singlet, duplicate, triplicate).

Available Simoa Immunoassays:

*The Representative Functional LLOQ is the assay’s Representative Analytical LLOQ corrected for a Recommended Sample Dilution Factor specific to each assay. 

Assay Species Matrix Representative Calibration Range Representative Functional LLOQ Datasheet
α-Synuclein
Alpha-Synuclein Discovery Kit Assay
Human

Serum

Plasma

CSF

4.12 - 1000 pg/mL

41.2 pg/mL Datasheet
Aβ-40
Amyloid Beta-40 Advantage Kit Assay
Human

Plasma

CSF

2.47-200 pg/mL

P: 4.92 pg/mL

CSF: 49.2 pg/mL

Datasheet
Aβ-42
Amyloid Beta-42 Advantage Kit Assay
Human

Plasma

CSF

0.137 - 100 pg/mL

P: 0.548 pg/mL

CSF: 1.37 pg/mL

Datasheet

BDNF
Brain Derived Neurotrophic Factor Discovery Kit Assay

Human

Serum

Plasma

CSF

0.029 - 120 pg/mL

S: 14.65 pg/mL

P: 14.65 pg/mL

CSF: 0.0586 pg/mL

Datasheet
c-MET
c-MET Discovery Kit Assay
Human

Serum

Plasma

Cell Lysates

0.488 pg/mL -2,000 pg/mL

97.6 pg/mL

Datasheet
C-peptide
Connecting Peptide Advantage Kit Assay
Human

Serum

Plasma

0.137 - 100 pg/mL 0.084 pg/mL Datasheet
CA-125
Cancer Antigen 125 Discovery Kit Assay
Human Serum 0.027 - 20 U/mL 0.10 U/mL  
CA-19-9
Cancer Antigen 19-9 Discovery Kit Assay
Human

Serum

Plasma

0.165 - 120 U/mL 0.082 U/mL Datasheet
Cathepsin S
Cathepsin S Discovery Kit Assay 
Human

Serum 

Plasma

1.95 – 2,000 pg/mL 195 pg/mL  Datasheet
CEA
Carcinoembryonic Antigen Discovery Kit Assay
Human

Serum

Plasma

2.33 – 1,700 pg/mL 116.5 pg/mL Datasheet
CRP
C-reactive Protein Discovery Kit Assay
Human

Serum

Plasma

1.37 – 1,000 pg/mL 32.92 pg/mL Datasheet
CXCL13
CXC Ligand 13 Protein Discovery Kit Assay
Human

Serum 

Plasma

0.274 - 200 pg/mL

0.28 pg/mL

Datasheet
Cytokine 3-Plex A
TNF-α, IL-6, IL-10
Human

Serum 

Plasma

IL-6: 1 - 15 pg/mL

IL-10: 1.02 - 6 pg/mL

TNFα: 1.02 – 28 pg/mL

IL-6: 0.044 pg/mL

IL-10: 0.029 pg/mL

TNFα: 2.04 pg/mL

Datasheet
Cytokine 3-Plex B
TNF-α, IL-6, IL-17A
Human

Serum

Plasma

IL-6: 1 - 15 pg/mL

IL-17A: 1 - 10 pg/mL

TNFα: 1.06 - 28 pg/mL

IL-6: 0.092 pg/mL

IL-17A: 0.0272

TNFα: 0.104 pg/mL

Datasheet
Eotaxin
Eotaxin Advantage Kit Assay
Human 

Serum 

Plasma

0.165 – 120 pg/mL 0.72 pg/mL Datasheet
G-CSF
Granulocyte Colony Stimulating Factor Advantage Kit Assay
Human

Serum

Plasma

0.274 - 200 pg/mL 0.38 pg/mL Datasheet
GFAP™
Glial Fibrillary Acidic Protein Discovery Kit Assay
Human

Serum

Plasma

CSF

1.37 – 1,000 pg/mL

S: 2.744 pg/mL

P: 2.744 pg/mL

CSF: 27.44 pg/mL

Datasheet
GM-CSF
Granulocyte Macrophage Colony Stimulating Factor Advantage Kit Assay
Human

Serum

Plasma

0.0411 – 30 pg/mL 0.0412 pg/mL Datasheet
HE4/WFDC2
Human Epididymis protein 4 Discovery Kit Assay
Human

Serum

Plasma

0.977 – 1,000 pg/mL 3.908 pg/mL Datasheet
IFN-α
Interferon Alpha Advantage Kit Assay
Human Serum 0.03 - 7.5 pg/mL 0.0094 pg/mL Datasheet
IFN-γ
Interferon Gamma Advantage Kit Assay
Human

Serum

Plasma

0.049 – 50 pg/mL 0.196 pg/mL Datasheet
IL-1α
Interleukin 1 Alpha Discovery Kit Assay
Human

Serum

Plasma

0.0412 pg/mL – 30 pg/mL 0.02 pg/mL Datasheet
IL-1β
Interleukin 1 Beta Advantage Kit Assay
Human

Serum

Plasma

0.165 -120 pg/mL 0.166 pg/mL Datasheet
IL-2
Interleukin 2 Advantage Kit Assay
Human

Serum

Plasma

0.123 – 30 pg/mL 0.164 pg/mL Datasheet
IL-3
Interleukin 3 Discovery Kit Assay
Human

Serum

Plasma

0.686 – 500 pg/mL 2.744 pg/mL Datasheet
IL-4
Interleukin 4 Advantage Kit Assay
Human

Serum

Plasma

0.137 - 100 pg/mL 0.078pg/mL Datasheet
IL-5
Interleukin 5 Advantage Kit Assay
Human

Serum

Plasma

0.016 – 12 pg/mL 0.033 pg/mL Datasheet
IL-6
Interleukin 6 Advantage Kit Assay
Human

Serum

Plasma

0.0412 – 30 pg/mL 0.04 pg/mL Datasheet
IL-7
Interleukin 7 Advantage Kit Assay
Human

Serum

Plasm

0.206 – 150 pg/mL 0.412 pg/mL Datasheet

IL-8
Interleukin 8 Advantage Kit Assay

Human

Serum

Plasma

0.073 – 300 pg/mL 0.3684 pg/mL Datasheet
IL-10
Interleukin 10 Advantage Kit Assay
Human

Serum

Plasma

0.0411 – 30 pg/mL 0.084 pg/mL Datasheet
IL-12p40/IL-23
Interleukin 8 Advantage Kit Assay
Human

Serum

Plasma

0.343 – 250 pg/mL 0.344 pg/mL Datasheet
IL-12p70
Interleukin 12p70 Advantage Kit Assay
Human

Serum

Plasma

0.0137 – 10 pg/mL 0.068 pg/mL Datasheet
IL-13
Interleukin 13 Advantage Kit Assay
Human

Serum

Plasma

0.004 – 15 pg/mL 0.01 pg/mL Datasheet
IL-15
Interleukin 15 Advantage Kit Assay
Human

Serum

Plasma

0.0137 – 10 pg/mL 0.0248 pg/mL Datasheet
IL-17A
Interleukin 17A Advantage Kit Assay
Human

Serum

Plasma

0.0412 – 30 pg/mL 0.084 pg/mL Datasheet
IL-17C
Interleukin 17A Discovery Kit Assay
Human

Serum 

Plasma

0.412 – 300 pg/mL 0.824 pg/mL Datasheet
IL-18
Interleukin 18 Discovery Kit Assay
Human

Serum

Plasma

0.011 – 45 pg/mL 0.6 pg/mL Datasheet
IL-22
Interleukin 22 Discovery Kit Assay
Human

Serum

Plasma

0.0412 – 30 pg/mL 0.0412 pg/mL Datasheet

IL-23/IL-12p40
Interleukin 23 Discovery Kit Assay

Human

Serum

Plasma

0.343 – 250 pg/mL 0.344 pg/mL Datasheet
IL-28A
Interleukin 28A Discovery Kit Assay
Human

Serum

Plasma

0.137 – 100 pg/mL 0.138 pg/mL Datasheet
IL-33
Interleukin 33 Discovery Kit Assay
Human

Serum 

Plasma

1.372 – 1,000 pg/mL 1.372 pg/mL Datasheet
IL-36β
Interleukin 36 Beta Discovery Kit Assay
Human

Serum

Plasma

0.206 – 150 pg/mL 0.824 pg/mL Datasheet

IP-10
Interferon Gamma Inducible Protein 10 Advantage Kit Assay

Human

Serum

 

0.274 – 200 pg/mL 0.708 pg/mL Datasheet

Leptin
Leptin Discovery Kit Assay

Human

Serum

Plasma

4.94 -1,200 pg/mL 247 pg/mL Datasheet
LIF
Leukemia Inhibitory Factor Discovery Kit Assay

Human

Serum

Plasma

0.178 – 130 pg/mL 0.344 pg/mL Datasheet
MCP-1
Monocyte Chemoattractant Protein-1 Advantage Kit Assay
Human

Serum

Plasma

0.823 – 200 pg/mL 0.612 pg/mL Datasheet
MCP-3
Monocyte Chemoattractant Protein-3 Discovery Kit Assay
Human

Serum

Plasma

0.309 – 225 pg/mL 0.618 pg/mL Datasheet
MIP-1β
Macrophage Inflammatory Protein 1B Discovery Kit Assay 
Human

Serum

Plasma

0.274 – 200 pg/mL 0.548 pg/mL Datasheet
MMP-9
Matrix metalloproteinase-9 Discovery Kit Assay
Human

Serum

Platelet-Poor Heparin Plasma

CSF

4.88 – 5,000 pg/mL

S: 4,880 pg/mL

P: 4,880 pg/mL

CSF: 39.04 pg/mL

 

Datasheet
Neurology 2-plex A
Aβ-42, Tau
Human

Serum

Plasma

CSF

Tau: 0.14 - 100 pg/mL

Aβ-42: 0.20 - 200 pg/mL

Tau: 0.268 pg/mL

Aβ-42: 0.684 pg/mL

Datasheet
Neurology 3-plex A
Aβ-40, Aβ-42, Tau
Human

Serum

Plasma

CSF

Tau: 0.13 - 100 pg/mL

Aβ-40: 1.4 - 140 pg/mL

Aβ-42: 0.15 - 60 pg/mL

Tau: 0.252 pg/mL

Aβ-40: 2.70 pg/mL

Aβ-42: 0.568 pg/mL

Datasheet
Neurology 4-plex A
NF-L, Tau, GFAP, UCH-L1
Human

Serum

Plasma

CSF

NF-L: 0.50 - 500 pg/mL

Tau: 0.1 - 100 pg/mL

GFAP: 1.0 - 1000 pg/mL

UCH-L1: 10 - 10000 pg/mL

NF-L: 0.964 pg/mL

Tau: 0.212 pg/mL

GFAP: 1.868 pg/mL

UCH-L1: 21.8 pg/mL

 

Datasheet

Neurology 4-plex B
NF-L, Tau, GFAP, UCH-L1

Human

Serum

Plasma

CSF

NF-L: 0.50 - 450 pg/mL

Tau: 0.1 - 90 pg/mL

GFAP: 10 - 9000 pg/mL

UCH-L1: 10 - 9000 pg/mL

NF-L: 2 pg/mL

Tau: 0.5 pg/mL

GFAP: 37.5 pg/mL

UCH-L1: 37.5 pg/mL

Datasheet

NF-LIGHT®
Neurofilament Light Advantage Kit Assay

Human

Serum

Plasma

CSF

0.5 - 450 pg/mL

S: 0.696 pg/mL

P: 0.696 pg/mL

CSF: 17.4 pg/mL

Datasheet

NSE
Neuron-Specific Enolase Discovery Kit Assay

 

Human

Serum

Plasma

9.88 – 2,400 pg/mL 494 pg/mL Datasheet
NT-proBNP
Discovery Kit Assay
Human

Serum

Plasma

0.206 – 50 pg/mL 2.06 pg/mL Datasheet
p-Tau181
Phospho-Tau181 Advantage Kit Assay
Human CSF 1.2 - 88 pg/mL 10.56 pg/mL Datasheet
p-Tau231
Phospho-Tau231 Advantage Kit Assay
Human CSF 0.41 - 300 pg/mL 7.32 pg/mL Datasheet

p24
Gag Protein p24 Advantage Kit Assay

Human

Serum

Plasma

0.01 - 30 pg/mL 0.01 pg/mL Datasheet
PD-1
Programmed-death Ligand Protein 1 Discovery Kit Assay 
Human

Serum

Plasma

1.76 – 1,800 pg/mL 3.516 pg/mL Datasheet
PD-L1
Programmed-death Ligand Protein 1 Discovery Kit Assay 
Human

Serum

Plasma

0.885 - 215 pg/mL 2.1 pg/mL Datasheet

PlGF
Placental Growth Factor Discovery Kit Assay

Human

Serum

Plasma

0.329 – 240 pg/mL 1.2 pg/mL Datasheet
pNF-heavy
Discovery Kit Assay
Human

Serum

Plasma

CSF

Mouse Serum

Mouse Plasma

2.88 – 2,100 pg/mL

S: 11.52 pg/mL

P: 11.52 pg/mL

CSF: 72 pg/mL

M-S: 28.8 pg/mL

M-P: 28.8 pg/mL

Datasheet

PSA
Prostate Specific Antigen Advantage Kit Assay

Human

Serum

Plasma

0.98 - 100 pg/mL 0.096 pg/mL Datasheet
Tau
Total Tau Advantage Kit Assay
Human

Serum

Plasma

CSF

1.05 - 100 pg/mL

S: 0.244 pg/mL

P: 0.244 pg/mL

CSF: 0.61 pg/mL

Datasheet

TDP43
TAR DNA Binding Protein Advantage Kit Assay

Human

Serum

Plasma

CSF

8.23 – 2,000 pg/mL 32.92 pg/mL Datasheet
TGF-α
Transforming Growth Factor Alpha Discovery Kit Assay
Human

Serum

Plasma

0.617 – 450 pg/mL 0.414 pg/mL Datasheet
TGF-β
Transforming Growth Factor Beta Discovery Kit Assay
Human

Serum

Plasma

2.06 – 1,500 pg/mL 8.224 pg/mL Datasheet

TNF-α
Tumor Necrosis Factor Alpha Advantage Kit Assay

Human

Serum

Plasma

0.069 – 50 pg/mL 0.136 pg/mL Datasheet
TNF-β
Tumor Necrosis Factor Beta Discovery Kit Assay
Human

Mouse Serum

Mouse Plasma

0.15 - 600 pg/mL 0.6 pg/mL Datasheet
TRAIL
TNF-related Apoptosis-Inducing Ligand Assay
Human

Serum

Plasma

0.137 – 100 pg/mL 0.0708 pg/mL Datasheet

Troponin-I
Cardiac Troponin-I (cTnI) Advantage Kit Assay

Human

Serum

Plasma

0.15 - 300 pg/mL 0.3 pg/mL Datasheet
UCH-L1™
Ubiquitin Carboxyl-Terminal Hydrolase L1 Discovery Kit Assay
Human

Serum

Plasma

CSF

6.86 – 5,000 pg/mL

S: 13.72 pg/mL

P: 13.72 pg/mL

CSF: 34.3 pg/mL

Datasheet
VEGF
Vascular Endothelial Growth Factor Discovery Kit Assay
Human Serum 0.274 – 200 pg/mL 0.548 pg/mL Datasheet
Mouse IL-1a
Mouse Interleukin 1 Alpha Discovery Kit Assay
Mouse

Serum

Plasma

0.096 – 70 pg/mL

0.60 pg/mL

 
Mouse IL-1b
Mouse Interleukin 1 Beta Discovery Kit Assay
Mouse

Serum

Plasma

0.041 – 30 pg/mL

0.082 pg/mL

 
Mouse IL-6
Mouse Interleukin 6 Discovery Kit Assay
Mouse

Mouse Serum

Plasma

0.240 – 170 pg/mL

0.480 pg/mL

Datasheet
Mouse IL-17A
Interleukin 17A Discovery Kit Assay
Mouse

Serum

Plasma

0.411– 300 pg/mL

1.648 pg/mL Datasheet
Mouse IL-17A/F
Interleukin 17A/F Discovery Kit Assay
Mouse

Serum

Plasma

0.27 – 200 pg/mL

0.274 pg/mL Datasheet
Mouse IL-17F
Interleukin 17F Discovery Kit Assay
Mouse

Serum

Plasma

0.82 – 600 pg/mL 1.648 pg/mL Datasheet
Mouse IL-22
Interleukin 22 Discovery Kit Assay
Mouse

Serum

Plasma

0.411 – 300  pg/mL

0.824 pg/mL Datasheet
Mouse IL-23
Interleukin 17F Discovery Kit Assay
Mouse

Serum

Plasma

0.27 – 200 pg/mL

0.548 pg/mL Datasheet
Mouse Tau
Tau Discovery Kit Assay
Mouse

Serum

Plasma

CSF

0.823 – 600 pg/mL

M-S: 3.29 pg/mL

M-P: 3.29 pg/mL

CSF: 32.92 pg/mL

Datasheet

Mouse TNF-α
Tumor Necrosis Factor Alpha Discovery Kit Assay

Mouse

Mouse Serum

Plasma

2.47 – 1800 pg/mL 4.92 pg/mL Datasheet

 

 

 

 

 

 

 

 

 

 

 

 

 

PBL's Ultrasensitive Biomarker Testing Assay Services take advantage of the benefits of Single Molecule Array (Simoa) technology. This approach traps a single bead in low femtoliter-sized wells to allow “digital” measurement of each bead for the presence of analyte in a sandwich immunoassay. The enzymatic amplification of analyte binding vents on single beads in single wells yields low background signals, and can increase assay sensitivities up to several orders of magnitude over conventional assays. Biomarker assays run on this platform frequently exhibit sensitivities in the low to mid fg/ml range.

 

In combination with the markedly heightened sensitivities of these assays, the instrument’s capacity for digital measurement at low concentrations of target analyte and analog measurement at higher analyte concentrations ensures accuracy and precision across a wide dynamic range (> 4 logs) minimizing sample volume requirements and the need for multiple dilutions. Allowing quantification of biomarkers at unprecedented levels in serum, plasma, and additional matrices, PBL’s Simoa-based Assay Services yield robust and reproducible data enabling scientists to attain a better understanding of the contributions of difficult to measure analytes to biological and pathological responses.

 

Powered by SimoaTM (Single Molecule Array) Technology, Quanterix, Lexington, MA, USA.

NF-light® is a registered trademark of Uman Diagnostics, Umeå Sweden.

 

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Technology Summary

Digital measurements are independent of intensity and simply rely on a signal/no signal readout on individual microbeads.

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