Cytokine and IFN Multiplex Assays

Why take advantage of a PBL Multiplex ELISA?

• Unique Human Type I, II, and III IFN & Cytokine panels allow rapid profiling of immune and inflammatory cytokine expression levels
• Robust technology for accurate analyte quantification in serum samples
• Cost-effective ELISA platform amenable to customization to include your analytes of interest
• Ready-to-run multiplex kits or testing services to obtain results more quickly

VeriPlex Mouse IFN 9-Plex ELISA Kit (52500-1)

Panel:

IFN-α, IFN-β, IFN-γ

IL-1α, IL-1β, IL-6, IL-10

RANTES, TNF-α

Matrix Compatibility: Serum, Plasma, & Tissue Culture Media

Assay Range: 2.86 log with LLOD and LLOQ in the low pg/ml range

Increased interferon (IFN) levels are a hallmark of many diseases, as members of this family of cytokines are sentinels in the innate immune response. High levels of Type I (IFN-Alpha & IFN-Beta) and Type III (IFN-Lambda) IFNs are produced upon host detection of nucleic acids during viral infections and some autoimmune diseases. Increases in Type II (IFN-Gamma) generally correlate with immune responses to intracellular pathogens and tumors.
 

In addition to IFNs, other cytokines also are indicators of nascent immune responses. Interleukins (IL-1Alpha, IL-6) and TNF-Alpha are endogenous pyrogens that are produced to raise the body temperature and help eliminate infections. Interleukins 6, and 8 (IL-6, IL-8) were increased in plasma from patients with ulcerative colitis, whereas IFN-Gamma levels were decreased.

Simultaneously analyzing the concentrations of multiple cytokines in a sample, and drawing correlations with expression profiles or disease severity may allow a global understanding of ongoing immune responses in diseases ranging from cancer to autoimmunity.