Quick Search
PBL’s improved site search function is defaulted to an exact match.
Search Term Results |
|
IFN beta |
All interferon beta products and related technical information (e.g. technical notes) |
Human IFN beta |
All human interferon products related technical information (e.g. technical notes) |
IL-2 |
All products contain the term “IL-2”, including IL-23 |
IL 2 |
All products contain the term “IL 2”, including IL-23 |
Erythropoietin |
All EPO products and technical information |
Eryth |
All EPO products and technical information |
The results are displayed in best match products grouped in the following product category filters:
Each filter contains sub-categories for further narrowing of your search results
Other web content such as technical notes that contain the Search Term can be displayed by checking the box next to the corresponding filter on the left side of the Search Results Page.
Authors: K. Abuarjah, E. Gonzalez, A. Mancino, K. O'Toole, H. Desai, C. Beaver
Author Affiliation: inVentiv Health Clinical
Presentation: 2012 AAPS poster
Purpose
Interferons (IFNs) are a group of cytokines used to treat Multiple Sclerosis. Four trademarked compounds (Rebif®, Avonex®, Betaseron®, and Extavia®) were independently validated using a commercial ELISA kit over a range of 5-200 pg/ml. Assay parameters were evaluated and optimized to improve the performance for the individual compounds.
Methods
The assay quantitates Human (IFN-beta) in serum by sandwich enzyme linked immunosorbent assay (ELISA) and has been developed to measure low/basal levels of IFN-beta. The Interferon binds to plates coated with antibody and the detection is accomplished using a biotinylated secondary antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). Tetramethy-benzidine (TMB) is the substrate. Prior to preparing the standards and QCs of the fortified solutions in human serum, it was necessary to screen the matrix in order to identify the matrices with below lower limit of quantitation (BQ) levels of endogenous analyte.
Results
The validation parameters of accuracy, precision, robutness, freeze-thaw stability, long-term stability, and bench-top stability were evaluated for each compound. The intra-run and inter-run (pooled) precision (%CV) and accuracy (%RE) for each validation sample concentration was ≤ 20% (≤ 25% for LLOQ and ULOQ) for the four compounds. The inter-run total error (|%CV| + |%RE|) was < 30% (< 40% for LLOQ)
Conclusion
Four sensitive assays for the detection of Rebif®, Avonex®, Betaseron®, and Extavia® were developed over a range of 5-200 pg/ml. The methods were reliable and robust, and considered suitable for the analyses of pharmacokinetic studies in human serum.
Click here to download poster
Find your lot-specific Certificate of Analysis here!
Finding the right biomarker detection service to meet your project needs just got easier...
Sign up for our email list to be kept up-to-date on the latest product releases and company news!
PBL works hand in hand with researchers to help solve difficult assay development and protein quantification problems. Known as a trusted source for high quality, high sensitivity ELISAs as well as interferon proteins and antibodies, PBL employs state-of-the-art technology in conducting assay services and manufacturing world-class products for scientists around the globe. Let us help you solve your most difficult problems... read more
Quick Search
PBL’s improved site search function is defaulted to an exact match.
Search Term Results |
|
IFN beta |
All interferon beta products and related technical information (e.g. technical notes) |
Human IFN beta |
All human interferon products related technical information (e.g. technical notes) |
IL-2 |
All products contain the term “IL-2”, including IL-23 |
IL 2 |
All products contain the term “IL 2”, including IL-23 |
Erythropoietin |
All EPO products and technical information |
Eryth |
All EPO products and technical information |
The results are displayed in best match products grouped in the following product category filters:
Each filter contains sub-categories for further narrowing of your search results
Other web content such as technical notes that contain the Search Term can be displayed by checking the box next to the corresponding filter on the left side of the Search Results Page.