Human Interleukin 15 / IL-15Rα heterodimer (Human Cell-Expressed)

Product Features: 
  • Recombinant Human IL-15 protein with high purity
  • Authentic human cell-expressed with no tag
  • Suitable for Direct Binding ELISA, Western Blot and CTLL-2 Proliferation Assay

Ordering

Catalog No. Pack Size Price Quantity
11702-2

50 µg

$450.00
11702-1

10 µg

$195.00

Specifications

Formulation Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Molecular Weight 31 kDa predicted (12.8 kDa for IL-15 + 18.4 kDa for sIL-15Rα). Heterodimer exhibits multiple bands on reducing SDS-PAGE due to varying degrees of glycosylation; migrates to a single band at ~87 kDa, observed in Native-PAGE
Source Recombinant DNA expressed in Human Embryonic Kidney Cells (HEK293)
Purity Greater than 95% as determined by SDS-PAGE by a combination of ion exchange and size exclusion chromatography
Endotoxin < 1 EU/µg
Bioactivity Typically <0.5 ng/ml (15.6 pM), measured by its dose dependent stimulation of the proliferation of CTLL-2 cells
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms IL-15, IL15, sIL-15Rα
Accession Number NP_002180 (IL-15); NP_002180.1 (sIL-15Rα)

Tech Info / Data

Reducing Western Blot of PBL's human cell-expressed Human IL-15 / sIL-15Ra Heterodimer (Cat. No. 11702) compared with HEK-expressed human IL-15 protein from Competitor A (Comp A) probed with anti-human IL-15Rα or anti-human IL-15 primary antibodies and Poly-HRP Steptavidin.

Tested Applications:

  • Direct Binding ELISA
  • Western Blot
  • CTLL-2 Proliferation Assay
Reducing SDS-PAGE (Left) and Native PAGE (Right) of PBL's human cell-expressed Human IL-15 / sIL-15Rα Heterodimer (Cat. No. 11702)

Reducing SDS-PAGE (Left) and Native PAGE (Right) of human cell-expressed Human IL-15 / sIL-15Rα Heterodimer (11702)

 

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