Human Interleukin 15 / IL-15Rα heterodimer (Human Cell-Expressed)
Specifications
| Formulation | Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
| Molecular Weight | 31 kDa predicted (12.8 kDa for IL-15 + 18.4 kDa for sIL-15Rα). Heterodimer exhibits multiple bands on reducing SDS-PAGE due to varying degrees of glycosylation; migrates to a single band at ~87 kDa, observed in Native-PAGE |
| Source | Recombinant DNA expressed in Human Embryonic Kidney Cells (HEK293) |
| Purity | Greater than 95% as determined by SDS-PAGE by a combination of ion exchange and size exclusion chromatography Endotoxin < 1 EU/µg |
| Bioactivity | Typically <0.5 ng/ml (15.6 pM), measured by its dose dependent stimulation of the proliferation of CTLL-2 cells |
| Storage | For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles |
| Synonyms | IL-15, IL15, sIL-15Rα |
| Accession Number | NP_002180 (IL-15); NP_002180.1 (sIL-15Rα) |
Tech Info / Data
Tested Applications:
- Direct Binding ELISA
- Western Blot
- CTLL-2 Proliferation Assay
Reducing SDS-PAGE (Left) and Native PAGE (Right) of human cell-expressed Human IL-15 / sIL-15Rα Heterodimer (11702)
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