Human LIF, Human Cell-Expressed (HCE)

Product Features: 
  • Authentic human glycosylation
  • Animal-component-free, protein-free production
  • Purity > 95%

Ordering

Catalog No. Pack Size Price Quantity
11646-4

1000 μg

$3,434.00
11646-3

100 μg

$1,309.00
11646-2

10 μg

$293.00

Specifications

Formulation Lyophilized in PBS
Molecular Weight 33 - 48 kDa, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically ≤ 0.2 ng/ml
Purity > 95% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11646 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human leukemia inhibitory factor (LIF) is a pleiotropic factor produced by numerous cell types which includes myelomonocytic lineages, T cells, fibroblasts, melanoma, liver, and heart. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. Mature human LIF (180 aa) shares 78%, 82%, 91%, 88%, and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. To reduce spontaneous differentiation, LIF is typically added to stem cell culture medium.

Human LIF Activity Assay

11646 Activity Assay

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