Quantitative Immunoassays

PBL's sample testing and screening services will expedite your research and development work. Our existing technologies and services can be tailored to meet your specific requirements under fit-for-purpose guidelines.

 

Whatever your sample testing and screening sensitivity needs, PBL can help you detect your analyte(s) of interest. From state of-the-art technologies quantifying into the femtogram/milliliter level to simultaneous detection of up to 16 analytes in a single well, PBL is a partner you can trust. We understand the value of your experimental samples and execute assays just as you would - consuming a minimum of sample while generating robust results. Let us help you develop, customize, or just execute assays on your samples in a timely, professional manner.

 


       

Selected capabilities:

 

     

Interested in one of our services?

 

Contact us:

W: online form

E: assayservices@pblassaysci.com

P: 1-877-PBL-8881

F: 1-732-777-9141

 

 


       

Ultra-Sensitive fg/ml Cytokine & Biomarker Quantification Services

 

Quanterix Simoa®

High precision. Unprecedented sensitivity. Digital readout. Take your detection to the next level.

 

  • Single Molecule Array technology delivers fg/ml level sensitivity for measurement of low abundance biomarkers in serum, plasma, and other matrices
  • Unique technology provides robust detection for single analytes and select multiplex panel options
  • High precision service offers accurate and reproducible data with < 10% inter- and intra-assay CVs
  • Over 50 analytes available including IL-17A, TNF-α, IL-23, TGF-β, TRAIL, IL-6, IL-10, IL-12p70, Aβ-40, Aβ-42, Tau, p-Tau231, and NF-LIGHT®

The unique immunoassay technology provided by the Simoa® platorm allows PBL to deliver fg/ml level sensitivity for low abundance biomarkers in serum, plasma, and other matrices. Utilizing Single Molecule Array technology which traps a single bead in a femtoliter-sized well allows for “digital” measurement of each bead. This approach can increase assay sensitivities several orders of magnitude over conventional bioassays.

 

The combination of this instrument’s markedly heightened sensitivity and its capacity for digital analyte measurement ensures accuracy and precision across a wide dynamic range and minimizes sample volume requirements.

 

For more information on PBL's Ultra-Sensitive fg/ml Biomarker Quantification Services, click here.

 

 

Singulex Erenna®

Accurate and robust measurement. Increase your understanding. Detect at unprecedented levels.

 

  • Cytokine analysis utilizing Single Molecule Counting (SMC) technology enables sub-pg/ml sensitivity for accurate measurement of low abundance biomarkers in normal or disease sera/plasma
  • Minimal sample dilution requirement with 4-Log+ dynamic range
  • High precision cytokine immunoassays provide reproducible data
  • Extensive analyte menu of over 30 different cytokine assays covering several disease areas including IFN-γ, IL-17A, IL-17F, IL-23, TNF-α, IL-6, and IL-10

The ability to accurately measure low-abundance analytes present in complex matrices is essential for the profiling and characterization of cytokines and other biomarker analytes. Employing proprietary single molecule counting (SMC) technology with robust microparticle-based Erenna® immunoassays, PBL’s cytokine detection services can provide scientists with sub-pg/ml level measurements of low-abundance analytes in healthy or disease sera and plasma.

 

An increased understanding of the role and regulation of cytokines and interferons in disease states results from greater profiling and characterization of their activities in biological responses. Singulex technology can be instrumental in furthering our collective understanding of low-abundance cytokines in complex matrices.

 

For more information on PBL's Ultra-Sensitive fg/ml Cytokine Testing Services, click here.

 

 

Single Analyte ELISA Service

Sensitive detection and robust results. Dedicated, flexible workforce. Reliable, high-quality outcomes.

 

Take advantage of PBL ELISAs or other commerical ELISAs for a wide range of additional analytes using PBL's services.

 

PBL ELISAs:

  • High sensitivity ELISAs available for a comprehensive range of species and analytes
  • Compatible with a variety of matrices including serum, plasma, tissue culture media (TCM), and cell lysates
  • Matrix tolerance for autoimmune disease sera*
  • Reproducible results with low intra- and inter-assay CVs

PBL ELISA kits are heterogeneous antibody sandwich immunoassays. The antigen is captured by an antibody immobilized to the wells in a microtiter plate and a secondary antibody is used to complete the sandwich. The detection system which utilizes horseradish peroxidase (HRP) and tetramethyl-benzidine (TMB) as the enzyme-substrate provides a sensitive and robust assay for the detection and quantification of interferons and cytokines.

 

PBL routinely runs ELISA studies for our clients for the detection and differentiation of interferons and cytokines in various sample matrices. We offer a range of heterogeneous immunoassay services for:

  • Human IFN-Alpha*, Beta*, Gamma, Lambda 1/2/3, Omega, Gamma Receptor 1
  • Mouse IFN-Alpha, Beta, Lambda 2/3
  • Cynomolgus/Rhesus IFN-Alpha, Beta

 

For more information on PBL's Single Analyte Service, click here.

 

 

Multiplex Screening Service

High sensitivity & specificity using low sample volumes. Cost-effective quantitation of multiple analytes.

 

  • Variety of chemokine and cytokine panels already available for human and mouse targets
  • Unique and customizable array of analytes including Type I, II & III IFNs, and other key cytokines crucial to innate and adaptive immunity
    • Compatible with autoimmune sera, plasma, tissue culture media (TCM), and buffer samples
    • Provides analyte sensitivity to as low as 5 pg/ml
    • Reproducible results with < 15% intra- and inter-assay CVs for most analytes in difficult matrices, including autoimmune serum and plasma

PBL offers cost-effective multiplex ELISA services that enable simultaneous detection of up to 16 different analytes in a single sample.

 

These ELISAs provide chemiluminescent results on each analyte and allow for a global understanding of ongoing immune responses in diseases ranging from cancer to autoimmunity. Evaluation of one given inflammatory molecule in the context of several others, repeated measurements of the same cytokine panels in the same subjects under different treatment assay conditions, and reliable detection of different proteins across a broad range of concentrations are just some of the benefits provided by this multiplex service.

 

For more information on PBL's Multiplex Screening Service, click here. 

     

Figure 1. Quanterix Simoa® Technology Overview

Quanterix Simoa Technology OverviewUnprecedented precision can be obtained using Simoa's "digital" readings of single molecular events. In the image above, individual paramagnetic beads are trapped in femtoliter-sized wells, then sealed over and quantified by SIMOA image analysis.

 

 

Figure 2. Singulex Erenna® Technology Overview

Singulex Erenna Technology Overview

 

Low abundance biomarkers in complex biological samples are transferred to a 96-well plate and incubated with capture antibody-coated microparticles. After washing and incubating with dye-labeled detection antibodes, wells are again washed to remove unbound detection antibodies. An elution buffer is added, and the eluate drawn into the instrument for single-molecule counting.

 

 

Figure 3. Multiplex Technology Overview

Multiplex Analyte Technology Overview

 

 

 

PBL's multiplex ELISAs employ a technology in which distinct capture antibodies are individually spotted into each well of a 96-well ELISA plate in a defined array. These plates are run similar to a normal ELISA run. An image of the plate is then taken using a CCD camera.

 

Using image analysis software to perform subsequent analysis, the chemiluminescent results for each analyte allow for a deeper understanding of the cytokine profiles of the samples.

 

 

To view an example of our sample testing report, click here.

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