Meso Scale Discovery Assay Services: MSD-ECL Assays

Advantages of Meso Scale Discovery Assay Services:

  • Ability to target low analyte levels in small sample volumes
  • Robust matrix tolerance including serum, plasma, and tissue culture media (TCM), as well as various complex biological matrices
  • Measure high and low abundance analytes (normal and elevated levels) within the same sample with 5-Log+ dynamic range
  • Over 180 validated fit-for-purpose singleplex and preconfigured multiplex panel ELISAs available with excellent performance specifications and lot-to-lot consistency

PBL offers high precision protein quantification services for biomarker detection in a broad range of sample matrices for Human, Non-Human Primate (NHP), Mouse, Rat, and Canine samples. PBL utilizes MSD's validated assay kits (V-Plex) to quantify up to 54 analytes within a single sample. This enables simultaneous comparison of the expression of pertinent cytokines, chemokines, and other biomarkers in samples from diseased and normal patients at different intervals.


Multiplex immunoassays on the MSD platform maintain the sensitivity and performance offered by singleplex ELISAs while providing additional benefits such as cost-savings and targeting of several related analytes in a single sample. The ability to quantify compatible analytes while requiring no more than 25 μl of neat sample ensures efficient use of precious sample.


Below is a sampling of the V-Plex panels currently available. Please contact our Assay Services team for tailored assistance regarding your specific analyte sample testing needs.


Human IL-3 standard curve on MSD platform

For additional information, please see the MSD-ECL Protein Detection Service Flyer.


Interested in our services?

Please include any project requirements or preferences including, for example, (1) number of samples, (2) sample matrix type(s), (3) volume of each sample, and (4) preferred number of replicates (e.g. singlet, duplicate, triplicate).

Selected MSD Multiplex Panels

General Category MSD V-Plex Panel Analytes

Human Cytokine & Chemokine Panels

V-Plex Cytokine Panel 1, 10-Plex

GM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A

V-Plex Proinflammatory Panel 1, 10-Plex

IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α

V-Plex Chemokine Panel 1, 11-Plex

Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC

V-Plex Th17 Panel 1, 7-Plex

IL-17A, IL-21, IL-22, IL-23, IL-27, IL-31, MIP-3α

V-Plex Human Cytokine, 30-Plex

Eotaxin, Eotaxin-3, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC, TNF-α, TNF-β, VEGF-A

V-Plex Human Cytokine, 36-Plex

Cytokines in 30-Plex plus IL-21, IL-22, IL-23, IL-27, IL-31, MIP-3α

V-Plex Human Cytokine, 44-Plex

Cytokines in 36-Plex plus IL-1RA,  IL-17A/F, IL-17B, IL-17D, TSLP

Human Biomarker Panels

V-Plex Human Biomarker Panel, 40-Plex

CRP, Eotaxin, Eotaxin-3, GM-CSF, FGF (basic), ICAM-1, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, PlGF, SAA, TARC, Tie-2,  TNF-α, TNF-β, VCAM-1, VEGF-A, VEGF-C, VEGF-D


V-Plex Human Biomarker Panel, 46-Plex

Cytokines in 40-Plex plus IL-21, IL-22, IL-23, IL-27, IL-31, MIP-3α


V-Plex Human Neuroinflammation Panel 1,


CRP, Eotaxin, Eotaxin-3, FGF (basic), ICAM-1, IFN-γ, IL-1αIL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-12p40, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, PIGF, SAA, TARC, Tie-2, TNF-α, TNF-β, VCAM-1, VEGF-A, VEGF-C, VEGF-D 

Mouse Cytokine Panel

V-Plex Mouse Cytokine Panel, 29-Plex

IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-15, IL-16, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, IP-10, MCP-1, MIP-1α, MIP-2, MIP-3α, TNF-α

Rat Cytokine Panel

V-Plex Proinflammatory Panel 2 Rat Kit

IFN-γ, IL-4, IL-5, IL-6, IL-10, IL-13, TNF-α

Non-Human Primate Cytokines & Chemokines Panel

V-Plex Cytokine 24-Plex NHP Kit

Eotaxin-3, IFN-γ, IL-1β, IL-2, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-15, IL-16, IL-17A, IP-10, GM-CSF, MCP-1, MCP-4, MCD, MIP-1α, MIP-1β, TARC, TNF-α, VEGF-A


Broad Range of Applications:


The unique detection technology provided by the MSD platform utilizes SULFO-TAG™ labels which emit light upon electrochemical stimulation. The intensity of light generated is captured via CCD camera and pixel intensities quantified. This system provides the basis for achieving measurement of a several log range of protein expression levels in a variety of matrices that cover a broad range of analytes, in normal and disease samples, as well as applications.


  • Cytokines & Chemokines
  • Cardiac Biomarkers
  • Immunogenicity
  • Immunology
  • Inflammation
  • Intracellular signaling
  • Metabolic
  • Neurodegeneration
  • Oncology and Cancer
  • Toxicity
  • ...and more


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IL-3 Stimulation using Jurkat Cells

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