Human IFN Alpha Multi-Subtype ELISA Kit (TCM)

Product Features: 
  • ELISA assay detects 11 of 12 human IFN alpha subtypes
  • ELISA assay quantifies human IFN-alpha 2 down to 12.5 pg/ml in tissue culture media (TCM)
  • Less than 10% inter and intra-assay CV

Full Product Name: VeriKine Human Interferon Alpha Multi-Subtype ELISA Kit

 

Looking for a TCM compatible All Subtype ELISA? Check out our NEW 41135 kit!

Ordering

Catalog No. Pack Size Price Quantity
41105-2

5 x 96-well plate

$2,775.00
$2,950.00
41105-1

1 x 96-well plate

$695.00

Specifications

Compatibility: Tissue Culture Media
ELISA Assay Range: 12.5 - 500 pg/ml or 156 - 5000 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Alpha (No cross-reactivity against human IFN-γ, IFN-β or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; bovine IFN-τ.)
Synonyms: IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
 
Inter-Assay CV: ≤ 8%
Intra-Assay CV: ≤ 8%
 
 
Storage: 2-8°C
Expiration Date: Twenty-one months from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Dilution Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • TMB Substrate
  • Stop Solution

Tech Info / Data

Human IFN alpha 2a extended range curve from 156 to 5000 pg/ml using PBL Human Interferon Alpha Multi-Subtype ELISA (41105)
Background: 

The VeriKine Human IFN-α Multi-Subtype ELISA kit is specifically formulated to detect 14 out of 15 identified human IFN-α subtypes/alleles in media using a sandwich immunoassay. They are: IFN-αA, IFN-α2, IFN-αD, IFN-αB2, IFN-αC, IFN-αG, IFN-αH, IFN-αI, IFN-αJ1, IFN-αK, IFN-α1, IFN-α4a, IFN-α4b, and IFN-αWA. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-α) provided by the National Institutes of Health.


Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. Generation of type I IFNs occurs downstream of a multitude of immunologic stimuli and can be either an indicator of host defense or an indicator of autoimmunity. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.

In humans, Interferon Alpha (IFN-alpha) is a family comprised of more than 12 homologous protein subtypes that exhibit diverse biologic activities. This group of proteins are greater than 85% homologous by amino acid sequence. Numerous individual human IFN-α subtypes have been identified; many display different properties. It remains unclear why there are multiple IFN-α subtypes. A variety of studies suggested they possess overlapping but also unique sets of biological activities. At least one subtype is used as a therapeutic, as a readout of drug efficacy in clinical trials, and as a marker of pathology in human clinical and preclinical studies. Elucidating the functional significances of various IFN-alpha subtypes can lead to further refinement of current interferon based therapeutic applications.

Citations

Typical Interferon Alpha Standard Curve from 12.5 pg/ml to 500 pg/ml Using PBL's Human IFN Alpha Multi-Subtype ELISA (41105)

 

Human IFN alpha 2a standard curve from 12.5 to 500 pg/ml using PBL Human Interferon Alpha Multi-Subtype ELISA (41105)

Assay Range: 12.5 - 500 pg/ml

Related Products

Catalog No. Description Pack Size Price Quantity
41135-1 VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit  new 
Compatibility: Tissue Culture Media
Assay Range: 1.95-125 pg/ml

1 x 96-well plate

$395.00
11105-1 Human Interferon Alpha 2 (Alpha 2b)

1 x 105 units

$240.00
41115-1 VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit
Compatibility: Serum, EDTA Plasma, Tissue Culture Media
Assay Range: 1.95-125 pg/ml

1 x 96-well plate

$745.00
$795.00
41100-1 VeriKine Human Interferon Alpha ELISA Kit
Compatibility: Tissue Culture Media
Assay Range: 12.5-500 pg/ml or 156-5000 pg/ml

1 x 96-well plate

$585.00
41110-1 VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 12.5-1000 pg/ml

1 x 96-well plate

$695.00

Find your product of interest by searching for category, species, molecule and/or application.

Visit our blog to read about recent developments in the fields of interferon, cytokine and biomarker research!

Sign up for our mailing list to be kept up-to-date on the latest promotions, company and product news.

Go to top