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Human IFN-Alpha Multi-Subtype ELISA Kit (TCM)

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Human IFN-Alpha Multi-Subtype ELISA Kit (TCM)

Catalog Number: 41105

This Human IFN-Alpha ELISA assay detects 11 of 12 IFN-Alpha subtypes with an assay range of 12.5-500 pg/ml or an extended assay range of 156-5000 pg/ml.


Click here to see which PBL Human IFN-Alpha ELISA kit is best fit for your research need.


Product Name: VeriKine Human Interferon-Alpha Multi-Subtype ELISA Kit (TCM)


Pack Size
Product Info

Matrix CompatibilityTissue Culture Media (TCM)
Assay Range12.5 - 500 pg/ml or 156 - 5000 pg/ml
Assay Length3 hours, 15 minutes
SpecificityHuman IFN-Alpha


The VeriKine Human IFN-Alpha Multi-Subtype ELISA kit is specifically formulated to detect 14 out of 15 identified human IFN-Alpha subtypes/alleles in media using a sandwich immunoassay. They are: IFN-AlphaA, IFN-Alpha2, IFN-AlphaD, IFN-AlphaB2, IFN-AlphaC, IFN-AlphaG, IFN-AlphaH, IFN-AlphaI, IFN-AlphaJ1, IFN-AlphaK, IFN-Alpha1, IFN-Alpha4a, IFN-Alpha4b, and IFN-AlphaWA.


The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human IFN-Alpha provided by the National Institutes of Health.


*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).


*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).



Inter-Assay: ≤ 8%

Intra-Assay: ≤ 8%


No cross-reactivity against

  • Human IFN-Gamma, IFN-Beta, IFN-Omega
  • Mouse or Rat IFN-Alpha, IFN-Beta, IFN-Gamma
  • Bovine IFN-Tau
SynonymsIFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
Expiration Date21 months from the date of manufacture
Shipping ConditionWet Ice



Materials Provided

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN-Alpha Standard, 10,000 pg/ml
  • Dilution Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • TMB Substrate
  • Stop Solution


Additional Materials Required (Not Provided) 

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker


Tech Info & Data

Tips, Tools and Troubleshooting:




Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.


In humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence. Numerous individual human IFN-α subtypes have been identified; many display different properties. It remains unclear why there are multiple IFN-α subtypes. A variety of studies suggested they possess overlapping but also unique sets of biological activities.


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Background Literature:


  1. Staehelin, T., Stähli, C., Hobbs, D.S., and Pestka, S. (1981) “A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies,” in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.
  2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) “A Sandwich Radioimmunoassay for Human IFNa” in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.
  3. Human IFN-α international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) “Interferon Standards and General Abbreviations,” in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23.
  4. Rubinstein, M., Levy, W.P., Moschera, J.A., Lai, C.-Y., Hershberg, R.D., Bartlett, R.T., and Pestka, S. (1981) “Human Leukocyte Interferon: Isolation and Characterization of Several Molecular Forms,” Arch. Biochem. Biophys. 210, 307-318.
  5. Hobbs, D.S. and Pestka, S. (1982) “Purification and Characterization of Interferons from a Continuous Myeloblastic Cell Line,” J. Biol. Chem. 257, 4071-4076.


Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
41105 Protocol (Full)

41105 Protocol (Full)

41105-1 CoA

41105-1 Certificate of Analysis (CoA)

41105-2 CoA

41105-2 Certificate of Analysis (CoA)

41105 SDS

41105 Safety Data Sheet