Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, TCM)

Product Features: 
  • Most sensitive ELISA on the market for human IFN alpha detection
  • Low pg/ml detection of ALL human IFN-α subtypes
  • Robust matrix tolerance including autoimmune serum, normal serum and tissue culture media (TCM)
  • Reproducible results with < 10% inter- and < 8% intra-assay CVs

Full Product Name: VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
41115-1

1 x 96-well plate

$735.00
$785.00

Specifications

Compatibility: Serum, Tissue Culture Media
ELISA Assay Range: 1.95 - 125 pg/ml
Speed: Typical Incubation time, 22 hours, 30 minutes
Specificity: Human Interferon Alpha
Cross-reacts with Cynomolgus/Rhesus IFN-α. No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; or bovine IFN-τ.
Synonyms: IFN alpha, IFN-a, Multisubtype Interferon Alpha, Multisubtype Leukocyte Interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
 
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 80% in Serum
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Sample Buffer
  • Standard Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Antibody Diluent
  • HRP Diluent
  • TMB Substrate
  • Stop Solution

Tech Info / Data

Image of global human interferon alpha-specific signals in autoimmune sera (n=26) using PBL's High Sensitivity Human Interferon Alpha All Subtype ELISA kit
Background: 

This high sensitivity Human IFN Alpha ELISA kit is designed to provide accurate low pg/ml measurement of ALL human interferon alpha subtypes in autoimmune sera, normal serum, and tissue culture media (TCM). This unique feature provides an improved view of global IFN-alpha production in sample analysis. 


Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory and many other activities.

In humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence. Numerous individual human IFN-α subtypes have been identified; many display different properties. It remains unclear why there are multiple IFN-α subtypes. A variety of studies suggested they possess overlapping but also unique sets of biological activities.

Citations

Representative Lower Limits of Quantitation and Lower Limits of Detection (pg/ml) in Normal Human Serum for each Human Interferon Alpha Subtype using PBL's High Sensitivity Human IFN Alpha ELISA (41115)

 

Image of table of representative LLOQs and LLODs in pg/ml in normal human serum for all human IFN alpha subtypes using PBL's High Sensitivity Human Interferon Alpha ELISA kit

Lower Limit of Quantitation (LLOQ) and Lower Limit of Detection (LLOD) for 41115

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