Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (TCM)

Product Features: 
  • Most sensitive ELISA on the market for global IFN alpha detection in tissue culture media (TCM)
  • Low pg/ml detection of ALL human IFN-α subtypes
  • Reproducible results with < 10% inter- and < 8% intra-assay CVs

Full Product Name: VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit

 

Looking for an autoimmune disease sera & TCM compatible All Subtype ELISA? Check out our 41115 kit!

Ordering

Catalog No. Pack Size Price Quantity
41135-1

1 x 96-well plate

$395.00

Specifications

Compatibility: Tissue Culture Media
ELISA Assay Range: 1.95 - 125 pg/ml
Assay Length: 20 hours 30 minutes - 24 hours 30 minutes
Specificity: Human Interferon Alpha
Cross-reacts with Cynomolgus/Rhesus IFN-α (13%). No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; pig IFN-α; or bovine IFN-τ.

 

Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Average Spike Recovery: 105%

 

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice


Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Assay Buffer
  • Standard Diluent
  • Antibody Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • HRP Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Typical Standard Curves of All 12 Human IFN Alpha Subtypes using PBL's High Sensitivity Human IFN Alpha All Subtype TCM ELISA (41135)
Lower Limits of Quantitation and Lower Limits of Detection of All 12 Human IFN Alpha Subtypes using PBL's High Sensitivity Human IFN Alpha All Subtype TCM ELISA (41135)
Linearity of Dilution in Three Different Tissue Culture Medias (TCM) using PBL's High Sensitivity Human IFN Alpha All Subtype TCM ELISA (41135)
Background: 

PBL’s VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit is specifically formulated to detect all human IFN-Alpha subtypes. This kit quantitates all human IFN alpha subtypes in tissue culture media (TCM) using a sandwich immunoassay format. The kit is based on an ELISA with biotinylated-detection antibody and streptavidin-conjugated horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha provided by the National Institutes of Health.


Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.

 

In humans, IFN-α refers to a family of proteins comprised of 12 highly homologous protein subtypes (greater than 85% by amino acid sequence), encoded by 13 genes, that exhibit pleiotropic biologic activities. Measuring the levels of the 12 different human IFN-α subtypes is essential for understanding their triggers and subsequent effects on the immune system. The ability to measure all the subtypes may provide an indication of the total IFN-α level.

Citations

Typical Standard Curves in Various Matrices using PBL's High Sensitivity Human IFN Alpha TCM ELISA (41135)

 

Typical Standard Curves in Various Matrices (Standard Diluent, RPMI, DMEM, MEM) using HS IFN Alpha TCM ELISA (41135)

Related Products

Catalog No. Description Pack Size Price Quantity
41115-1 VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit
Compatibility: Serum, EDTA Plasma, Tissue Culture Media
Assay Range: 1.95-125 pg/ml

1 x 96-well plate

$745.00
$795.00

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