Human IFN Beta TCM ELISA, High Sensitivity

Product Features: 
  • Human IFN-Beta ELISA detects the amount of interferon beta in tissue culture media (TCM) to as low as 2.34 pg/ml
  • Total IFN-Beta quantification with no interference from soluble IFN receptor (sIFNAR2)
  • Reproducible results with inter- and intra-assay CVs < 10%

Product Full Name: VeriKine-HS Human IFN Beta TCM ELISA Kit

 

Note: This kit is not intended for use with human serum or plasma samples due to a significantly elevated risk of false-positive results.

 

Looking for a serum & plasma compatible IFN-Beta ELISA? We recommend the 41415 immunoassay!

Ordering

Catalog No. Pack Size Price Quantity
41435-1

1 x 96-well plate

$450.00

Specifications

Compatibility: Tissue Culture Media (TCM) only
ELISA Assay Range:  2.34 - 150 pg/ml
Speed: Assay time 3 hours
Specificity: Human IFN Beta 1a & 1b, No cross-reactivity with human IFN-alpha, IFN-gamma, IFN-lambda, IL-6; mouse IFN-alpha, IFN-beta; or rat IFN-beta
Synonyms: Human Beta Interferon, Human Fibroblast IFN, Human Type I Interferon Beta, Human IFNb

 

Inter-Assay CV: < 8%
Intra-Assay CV: < 10%

 

Storage: 2-8 ºC
Expiration Date: 12 months from date of manufacture
Shipping Condition: Wet Ice


Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN Beta 1a Standard, 100,000 pg/ml
  • Standard Diluent
  • Sample Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Assay Diluent
  • TMB Substrate
  • Stop Solution

Tech Info / Data

Induction of Human IFN-Beta by TLR-7/8 Agonist Stimulation of PBMCs
Representative Standard Curves in Various Matrices for PBL's VeriKine-HS Human IFN-Beta TCM ELISA Kit (Catalog No. 41435)
Background: 

The VeriKine-HS Human IFN-Beta TCM ELISA Kit is designed for accurate quantification of low level human interferon beta in buffers or tissue culture media (TCM) by sandwich ELISA. The lowest concentration of Hu IFN-Beta is 2.34 pg/ml. This kit is not intended for use with human serum or plasma samples due to a significantly elevated risk of false positive results. For complex biological matrices such as plasma, healthy, and disease sera, we recommend our High Sensitivity Human IFN Beta Serum ELISA Kit (Cat. No. 41415-1).


Interferon Beta (IFN-Beta, IFNb) is synthesized and secreted by fibroblasts and many other cell types in response to varied stimuli in Toll-like receptor (TLR) dependent and independent mechanisms. Pathogen exposure can result in the activation of transcription factors interferon regulatory factor 3 (IRF3) and NF-kB resulting in IFN-Beta production in a stimuli and cell-dependent manner. Conversely, IFN-Beta aberrant expression may be associated with inflammation and autoimmunity.

Parallelism of Endogenous Samples using PBL's High Sensitivity Human IFN-Beta TCM ELISA (41435)

Parallelism of endogenous samples using PBL's Human IFN Beta TCM ELISA (41435)

Five endogenous samples were tested in duplicate for assay parallelism. Samples were serially diluted in TCM to the 32-fold dilution that lies within the kit's assay range. Percent recoveries are 100 ± 20% of neat value except for the 32nd dilution of Sample 5 (S5) which falls marginally out of the assay's LLOQ (2.34 pg/ml).

Related Products

Catalog No. Description Pack Size Price Quantity
41415-1 VeriKine-HS Human Interferon Beta Serum ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 1.2-150 pg/ml

1 x 96-well plate

$775.00
41410-1 VeriKine Human Interferon Beta ELISA Kit
Compatibility: Tissue Culture Media
Assay Range: 50-4000 pg/ml

1 x 96-well plate

$685.00
41410-2 VeriKine Human Interferon Beta ELISA Kit
Compatibility: Tissue Culture Media
Assay Range: 50-4000 pg/ml

5 x 96-well plate

$2,595.00
$2,850.00

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