The VeriKine-HS Human IFN-Beta TCM ELISA Kit is designed for accurate quantification of low-level human interferon beta in buffers or tissue culture media (TCM) by sandwich ELISA. The LLOQ of this kit is 2.34 pg/ml.

Note: This kit is not intended for use with human serum or plasma samples due to a significantly elevated risk of false-positive results.

*For complex biological matrices such as plasma, healthy sera, and disease sera, we recommend our Human IFN-Beta Serum ELISA Kit, High Sensitivity (Cat. No. 41415-1).

Materials Provided

• Pre-coated microtiter plate
• Plate Sealers
• Wash Solution Concentrate
• Human IFN Beta 1a Standard, 100,000 pg/ml
• Standard Diluent
• Sample Buffer
• Antibody Concentrate
• HRP Conjugate Concentrate
• Assay Diluent
• TMB Substrate
• Stop Solution

Additional Materials Required (Not Provided) 

• Microplate reader capable of reading an OD at a wavelength of 450 nm
• Variable volume microtiter pipettes
• Adjustable multichannel pipette (50-300 μl)
• Reagent reservoirs
• Wash bottle or plate washing system
• Distilled or deionized water
• Serological pipettes (1, 5, 10 or 25 ml)
• Disposable pipette tips (polypropylene)
• Plate shaker

Representative Human IFN-Beta Standard Curves in Various Matrices

Intra and Inter-Assay CVs to measure Precision
Matrix	Standard Diluent	TCM
n	7	18
Intra-Assay CV (%)	4.10	4.06
Inter-Assay CV (%)	2.33	3.16

 

Spike Recovery
TC Media Spike Sample	1	2	3
Target Conc. (pg/ml)	100	25	5
Mean Recovery (%)	101.1	95.7	91.4
Range (%)	97-106	90-102	85-96

 

 

Matrix Compliance for Standard Diluent and Three Different TCM

 

Parallelism of Five Endogenous Samples

 

Sensitivity in Stimulated PBMC Samples

 

Endogenous Levels of IFN-Beta Quantified in Sendai Virus-Induced HEK293 Cells

 

Representative Standard Curves

 

 

sIFNAR2 Receptor Interference in Competitor A’s ELISA

 

sIFNAR2 Receptor Interference in Competitor A’s ELISA

 

sIFNAR2 Receptor Effect in PBL’s 41435 ELISA

 

Quantitation of Total IFN-Beta in the presence of sIFNAR2

 

Background

 

Interferon Beta (IFN-Beta, IFNb) is synthesized and secreted by fibroblasts and many other cell types in response to varied stimuli in Toll-like receptor (TLR) dependent and independent mechanisms. Pathogen exposure can result in the activation of transcription factors interferon regulatory factor 3 (IRF3) and NF-kB resulting in IFN-Beta production in a stimuli and cell-dependent manner. Conversely, IFN-Beta aberrant expression may be associated with inflammation and autoimmunity.

 

5 Citations:

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