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Human IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

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Human IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

Catalog Number: 41415

This ELISA measures human IFN-Beta levels in serum, plasma, and tissue culture media (TCM) with 1.2 pg/ml LLOQ sensitivity. It is suitable for the measurement of IFN-Beta in autoimmune serum and of trademarked IFN-Beta 1a and IFN-Beta 1b therapeutic molecules in human serum. There is no detection inhibition by 3-log excess of soluble IFNAR2 protein.


Product Name: VeriKine-HS Human Interferon-Beta ELISA Kit (Serum, Plasma, TCM)


Pack Size
Product Info

Matrix Compatibility Serum, Plasma, Tissue Culture Media (TCM)
Assay Range

Protocol A: 1.2 - 150 pg/ml (for improved serum performance)

Protocol B: 2.3 - 150 pg/ml


1.2 pg/ml

Need more sensitivity? Check out our Sample Testing Services

Assay Length

Protocol A: 3 hours, 30 minutes

Protocol B: 3 hours

Specificity Human IFN-β


The VeriKine-HS Human IFN-Beta Serum ELISA Kit is developed to measure low or basal levels of Human IFN-Beta in autoimmune sera, normal sera/plasma, or tissue culture media (TCM) samples by sandwich ELISA. The LLOQ of this kit is 1.2 pg/ml. Basal levels of Type I IFNs, including IFN-Beta, are not fully understood. They are believed to be important for robust response to pathogens and may play additional roles in cellular homeostasis.


This assay is suitable for the measurement of trademarked therapeutic molecules in human serum samples. Researchers and clinical investigators examining a) the pharmacokinetics of IFN-Beta molecules, b) IFN-Beta as a biomarker, or c) IFN-Beta as a pharmacodynamic marker of TLR agent or other immune response modifier activity will find this immunoassay to be an essential laboratory tool.


*For a TCM-compatible ELISA, we recommend our Human IFN-Beta ELISA Kit, High Sensitivity (Cat. No. 41435-1).


CVs and Spike Recovery

Inter-Assay < 8%

Intra-Assay < 10%


Spike Recovery > 90% in Serum



No cross-reactivity detected with:

  • Human IFN-α, IFN-γ, IFN-ω, or IL-6
  • Mouse IFN-α, IFN-β
  • Rat IFN-β
Synonyms Human Beta Interferon, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B
Storage 2-8°C
Expiration Date 12 months from the date of manufacture
Shipping Condition Wet Ice



Materials Provided

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Beta 1a Standard, 100,000 pg/ml
  • Standard Diluent
  • Sample Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Diluent Additive III (for use in Protocol A)
  • Assay Diluent
  • TMB Substrate
  • Stop Solution


Additional Materials Required (Not Provided)

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data



Interferon beta (IFN-Beta, IFNb) is part of the first wave of cytokine response in cells. Pathogen infection can result in the activation of interferon regulatory factor 3 (IRF3) which functions in trans to activate IFN-Beta gene transcription. IFN-Beta is biologically unique when compared to other interferons and studies have shown that IFN-Beta has overlapping and distinct gene expression patterns as compared to IFN-Alpha. It appears that IFN-Beta binds to the Type I IFN receptor with higher affinity than the other Type I IFNs and it may also regulate receptor internalization in a different manner. Additionally, IFN-Beta has long been known to inhibit viral replication as part of the body’s innate antiviral response and is used as a therapeutic for the treatment of Multiple Sclerosis (MS) and some tumors.




  1. Performance Characterization Of A High Sensitivity Human Interferon Beta ELISA Kit In Healthy Serum, Patient Serum And Plasma Samples (link)

  2. Validation of a Highly Sensitive Immunoassay for the Quantitation of Interferon Beta in Autoimmune Sera (link)

  3. Optimization and Validation of an ELISA kit for the Quantification of Four Interferon-Beta (IFN-β) Marketed Compounds in Human Serum (link)



49 Citations


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  9. Terajima, H et al., (2021), N6-methyladenosine promotes induction of ADAR1-mediated A-to-I RNA editing to supress aberrant antiviral innate immune response, PLoS Biol., 19(7):e3001292, PMID:34324489 (link)

  10. Blanco-Melo, D. et al., (2020), Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19, Cell, 181(5):1036, PMID: 32416070, DOI: 10.1016/j.cell.2020.04.026 (link)

  11. Dubey, et al. (2020). Specific protein-protein interactions limit the cutaneous iontophoretic transport of interferon beta-1B and a poly-ARG interferon beta-1B analogue. International Journal of Pharmaceutics, 6 pgs. PMID: no PMID. (link)

  12. Newling, et al. (2019). Dysregulated Fcγ receptor IIa-induced cytokine production in dendritic cells of lupus nephritis patients. Clinical and Experimental Immunology, 11 pgs. PMID: 31509231. link)

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  15. Gannon, et al. (2018). Identification of ADAR1 adenosine deaminase dependency in a subset of cancer cells. Nature Communications, 10 pgs. PMID: 30575730. (link)

  16. Colavita, Francesca, et al. (2018). Overproduction of IL-6 and Type-I IFN in a Lethal Case of Chikungunya Virus Infection in an Elderly Man During the 2017 Italian Outbreak. Open Forum Infectious Diseases, 21 pgs. PMID: 30539034. (link)

  17. Merindol, Natacha, et al. (2018). HIV-1 Capsids from B27/B57+ Elite Controllers Escape Mx2 but are Targeted by TRIM5-alpha, Leading to the Induction of an Antiviral State. PLOS Pathogens, 22 pgs. PMID: 30419009. (link)

  18. Zhang, Guoliang, et al. (2018). A proline deletion in IFNAR1 impairs IFN-signaling and underlies increased resistance to tuberculosis in humans. Nature Communications, 9 pgs. PMID: 29311663. (link)

  19. Ager, Casey (2018). Intratumoral STING Activation Sensitizes Poorly Immunogenic Cancers to Checkpoint Blockade Immunotherapy. University of Texas, 229 pgs. PMID: no PMID. (link)

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  21. Kondoh, Tatsunari, et al. (2017). Putative endogenous filovirus VP35-like protein potentially functions as an IFN antagonist but not a polymerase cofactor. PLOS One, 17 pgs. PMID: 29040311. (link)

  22. Brown, Michael, et al. (2017). Cancer Immunotherapy with Recombinant Poliovirus Induces IFN-Dominant Activation of Dendritic Cells and Tumor Antigen-Specific CTLs. Sci Transl Med, 31 pgs. PMID: 28931654. (link)

  23. Cheng, Liang, et al. (2017). Type I interferons suppress viral replication but contribute to T cell depletion and dysfunction during chronic HIV-1 infection. JCI Insight, 13 pgs. PMID: 28614789. (link)

  24. Luan, Liming, et al. (2017). Comparative Transcriptome Profiles of Human Blood in Response to the Toll-like Receptor 4 Ligands Lipopolysaccharide and Monophosphoryl Lipid A. Scientific Reports, 16 pgs. PMID: 28053314. (link)

  25. Gusella, Luca, et al. (2016). Prothymosin-α Variants Elicit Anti-HIV-1 Response via TLR4 Dependent and Independent Pathways. PLOS One, 17 pgs. PMID: 27310139. (link)

  26. GuhaSarkar, Dwijit, et al. (2016). Systemic AAV9-IFNβ gene delivery treats highly invasive glioblastoma. Neuro-Oncology, 11 pgs. PMID: 27194146. (link)

  27. Vanheule, Vincent, et al. (2016). Basic chemokine-derived glycosaminoglycan binding peptides exert antiviral properties against dengue virus serotype 2, herpes simplex virus-1 and respiratory syncytial virus. Biochemical Pharmacology, pgs. PMID: 26551597. (link)

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  29. Dauletbaev, Nurlan, et al. (2015). Stimulation of the RIG-I/MAVS Pathway by Polyinosinic:Polycytidylic Acid Upregulates IFN-β in Airway Epithelial Cells with Minimal Costimulation of IL-8. Journal of Immunology, 14 pgs. PMID: 26283481. (link)

  30. Spengler, Jessica, et al. (2015). RIG-I Mediates an Antiviral Response to Crimean-Congo Hemorrhagic Fever Virus. JVI, 11 pgs. PMID: 26223644. (link)

  31. Eigenbrod, Tatjana, et al. (2015). TLR8 Senses Bacterial RNA in Human Monocytes and Plays a Nonredundant Role for Recognition of Streptococcus pyogenes. Journal of Immunology, 9 pgs. PMID: 26101323. (link)

  32. Bergstrom, Bjarte, et al. (2015). TLR8 Senses Staphylococcus aureus RNA in Human Primary Monocytes and Macrophages and Induces IFN-β Production via a TAK1-IKKβ-IRF5 Signaling Pathway. Journal of Immunology, 13 pgs. PMID: 26085680. (link)

  33. Olaisen, Camilla, et al. (2015). PCNA-interacting peptides reduce Akt phosphorylation and TLR-mediated cytokine secretion suggesting a role of PCNA in cellular signaling. Cellular Signaling, 10 pgs. PMID: 25797046. (link)

  34. White, Michael, et al. (2014). Apoptotic Caspases Suppress mtDNA-Induced STING-Mediated Type I IFN Production. CellPress, 27 pgs. PMID: 25525874. (link)

  35. Wolferstaetter, Michael, et al. (2014). Recombinant Modified Vaccinia Virus Ankara Generating Excess Early Double-Stranded RNA Transiently Activates Protein Kinase R and Triggers Enhanced Innate Immune Responses. JVI, 16 pgs. PMID: 25297997. (link)

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  38. Lee, et al. (2013). xSite-Specific PEGylation Enhances the Pharmacokinetic Properties and Antitumor Activity of Interferon Beta-1b. Journal of Interferon & Cytokine Research, pgs. PMID: 23962003. (link)

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  40. Weix, et al. (2013). The Physiologic Increase in Expression of Some Type I IFN-Inducible Genes During Pregnancy is Not Associated with Improved Disease Activity in Pregnant Patients with Rheumatoid Arthritis. Translational Research. PMID: no PMID. (link)

  41. Wang, et al. (2012). Application of Secretary Luciferase Labeled Orthotopic Transplant Model of Hepatocellular Carcinoma to Evaluate Tumor Response to Interferon-beta Gene Therapy. Sheng Wu Gong Cheng Xue Bao. PMID: 23311138. (link)

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Background Literature:


  1. Krause CD, Pestka S. Evolution of the Class 2 cytokines and receptors, and discovery of new friends and relatives. Pharmacol Ther. 2005 Jun;106(3):299-346.

  2. Weinstock-Guttman B, Ramanathan M, Zivadinov R. Interferon-beta treatment for relapsing multiple sclerosis. Expert Opin Biol Ther. 2008 Sep;8(9):1435-47.

  3. Lee MS, Kim YJ. Pattern-recognition receptor signaling initiated from extracellular, membrane, and cytoplasmic space. Mol Cells. 2007 Feb 28;23(1):1-10.

  4. Heim MH. The Jak-STAT pathway: cytokine signaling from the receptor to the nucleus. J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):75-120.

  5. Taniguchi T, Takaoka A. The interferon-alpha/beta system in antiviral responses: a multimodal machinery of gene regulation by the IRF family of transcription factors. Curr Opin Immunol. 2002 Feb;14(1):111-6.

  6. Lewerenz M, Mogensen KE, Uzé G. Shared receptor components but distinct complexes for alpha and beta interferons. J Mol Biol. 1998 Sep 25;282(3):585-99.

  7. Jaks E, Gavutis M, Uzé G, Martal J, Piehler J. Differential receptor subunit affinities of type I interferons govern differential signal activation. J Mol Biol. 2007 Feb 16;366 (2):525- 39.

  8. Marijanovic Z, Ragimbeau J, van der Heyden J, Uzé G, Pellegrini S. Comparable potency of IFNalpha2 and IFN beta on immediate JAK/STAT activation but differential downregulation of IFNAR2. Biochem J. 2007 Oct 1;407(1):141-51.

  9. Taniguchi T, Takaoka A. A weak signal for strong responses: interferon-alpha/beta revisited. Nat Rev Mol Cell Biol. 2001 May; 2(5):378-86.

  10. Aziz N, Nishanian P, Mitsuyasu R, Detels R, Fahey JL. Variables That Affect Assays for Plasma Cytokines and Soluble Activation Markers. Clin Diagn Lab Immunol. 1999 Jan: (6)1:89-95.


Technical Data Sheet, Certificate of Analysis (CoA), Protocol, and Safety Data Sheet (SDS)
41415 TDS

41415 Technical Data Sheet

41415 CoA and Protocol A

41415 Certificate of Analysis and Protocol A

41415 CoA and Protocol B

41415 Certificate of Analysis and Protocol B

41415 SDS

41415 SDS

41415 Poster

Performance Characterization of a High Sensitivity Human Interferon Beta ELISA Kit in Healthy Serum, Patient Serum and Plasma Samples

VeriKine-HS Human IFN-Beta ELISA Product Flyer

Allows accurate Human IFN-β measurement to as low as 1.2 pg/ml in human serum, plasma, autoimmune disease sera or cell culture media

41415 Product Notice

41415 Product Notice

41415 Protocol A

41415 Protocol A

41415 Protocol B

41415 Protocol B