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Human IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

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Human IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

Catalog Number: 41415

This Human IFN-Beta ELISA measures interferon beta levels in human serum, plasma, and tissue culture samples with 1.2 pg/ml LLOQ sensitivity. This kit has been validated for the measurement of IFN-Beta in autoimmune serum and of trademarked IFN-Beta 1a and IFN-Beta 1b therapeutic molecules in human serum. There is no detection inhibition by 3-log excess of soluble IFNAR2 protein.

 

Product Name: VeriKine-HS Human Interferon-Beta ELISA Kit (Serum, Plasma, TCM)

$725.00

Pack Size
Product Info

Matrix Compatibility Serum, Plasma, Tissue Culture Media (TCM)
Assay Range

Protocol A: 1.2 - 150 pg/ml (for improved serum performance)

Protocol B: 2.3 - 150 pg/ml

LLOQ

1.2 pg/ml

Need more sensitivity? Check out our Sample Testing Services

Assay Length

Protocol A: 3 hours, 30 minutes

Protocol B: 3 hours

Specificity Human IFN-β

 

The VeriKine-HS Human IFN-Beta ELISA Kit is designed to measure low or basal levels of Human IFN-Beta in autoimmune disease sera, healthy serum/plasma, or tissue culture media samples with 1.2 pg/ml LLOQ sensitivity.

 

This assay is suitable for the measurement of trademarked therapeutic molecules in human serum samples. Researchers and clinical investigators examining a) the pharmacokinetics of IFN-Beta molecules, b) IFN-Beta as a biomarker, or c) IFN-Beta as a pharmacodynamic marker of TLR agent or other immune response modifier activity will find this immunoassay to be an essential laboratory tool.

 

*For a TCM-compatible ELISA, we recommend our Human IFN-Beta ELISA Kit, High Sensitivity (Cat. No. 41435-1).

Specifications

CVs and Spike Recovery

Inter-Assay < 8%

Intra-Assay < 10%

 

Spike Recovery > 90% in Serum

Cross-reactivity

 

No cross-reactivity detected with:

  • Human IFN-α, IFN-γ, IFN-ω, or IL-6
  • Mouse IFN-α, IFN-β
  • Rat IFN-β
Synonyms Human Beta Interferon, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B
Storage 2-8°C
Expiration Date 12 months from the date of manufacture
Shipping Condition Wet Ice

 

 

Materials Provided

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Beta 1a Standard, 100,000 pg/ml
  • Standard Diluent
  • Sample Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Diluent Additive III (for use in Protocol A)
  • Assay Diluent
  • TMB Substrate
  • Stop Solution

 

Additional Materials Required (Not Provided)

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data

Background

 

Interferon beta (IFN-Beta, IFNb) is part of the first wave of cytokine response in cells. Pathogen infection can result in the activation of interferon regulatory factor 3 (IRF3) which functions in trans to activate IFN-Beta gene transcription. IFN-Beta is biologically unique when compared to other interferons and studies have shown that IFN-Beta has overlapping and distinct gene expression patterns as compared to IFN-Alpha. It appears that IFN-Beta binds to the Type I IFN receptor with higher affinity than the other Type I IFNs and it may also regulate receptor internalization in a different manner. Additionally, IFN-Beta has long been known to inhibit viral replication as part of the body’s innate antiviral response and is used as a therapeutic for the treatment of Multiple Sclerosis (MS) and some tumors.

Citations

Posters

 

  1. Performance Characterization Of A High Sensitivity Human Interferon Beta ELISA Kit In Healthy Serum, Patient Serum And Plasma Samples (link)

  2. Validation of a Highly Sensitive Immunoassay for the Quantitation of Interferon Beta in Autoimmune Sera (link)

  3. Optimization and Validation of an ELISA kit for the Quantification of Four Interferon-Beta (IFN-β) Marketed Compounds in Human Serum (link)

 

 

53 Citations

 

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  2. Grunhagel, B., et al., (2023), "Reduction of IFN-I Responses by Plasmacytoid Dendritic Cells in a Longitudinal Trans Men Cohort, iScience, DOI: 10.1016/j.isci.2023.108209 (link)

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  4. Ullah, T.R. et al., (2023), "Pharmacological inhibition of TBK1/IKKε blunts immunopathology in a murine model of SARS-CoV-2 infection", Nat Commun. 14(1):5666, PMID: 37723181, DOI: 10.1038/s41467-023-41381-9 (link)

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  12. Dorgham, et al. (2021). Considering Personalized Interferon Beta Therapy for COVID-19. Antimicrobial Agents and Chemotherapy, 3 pgs. PMID: 21321205. (link)

  13. Contoli, M. et al., (2021), Bllod Interferon-a Levels and Severity, Outcomes, and Inflammatory Profiles in Hospitalized COVID-19 Patients, Front. Immunol., PMID: 33767713, DOI: 10.3389/fimmu.2021.648004 (link)

  14. Terajima, H et al., (2021), N6-methyladenosine promotes induction of ADAR1-mediated A-to-I RNA editing to supress aberrant antiviral innate immune response, PLoS Biol., 19(7):e3001292, PMID:34324489 (link)

  15. Blanco-Melo, D. et al., (2020), Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19, Cell, 181(5):1036, PMID: 32416070, DOI: 10.1016/j.cell.2020.04.026 (link)

  16. Dubey, et al. (2020). Specific protein-protein interactions limit the cutaneous iontophoretic transport of interferon beta-1B and a poly-ARG interferon beta-1B analogue. International Journal of Pharmaceutics, 6 pgs. PMID: no PMID. (link)

  17. Newling, et al. (2019). Dysregulated Fcγ receptor IIa-induced cytokine production in dendritic cells of lupus nephritis patients. Clinical and Experimental Immunology, 11 pgs. PMID: 31509231. link)

  18. Skjeflo, et al. (2019). Phagocytosis of live and dead Escherichia coli and Staphylococcus aureus in human whole blood is markedly reduced by combined inhibition of C5aR1 and CD14. Molecular Immunology, 9 pgs. PMID: 31102985. (link)

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  22. Merindol, Natacha, et al. (2018). HIV-1 Capsids from B27/B57+ Elite Controllers Escape Mx2 but are Targeted by TRIM5-alpha, Leading to the Induction of an Antiviral State. PLOS Pathogens, 22 pgs. PMID: 30419009. (link)

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  24. Ager, Casey (2018). Intratumoral STING Activation Sensitizes Poorly Immunogenic Cancers to Checkpoint Blockade Immunotherapy. University of Texas, 229 pgs. PMID: no PMID. (link)

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  26. Kondoh, Tatsunari, et al. (2017). Putative endogenous filovirus VP35-like protein potentially functions as an IFN antagonist but not a polymerase cofactor. PLOS One, 17 pgs. PMID: 29040311. (link)

  27. Brown, Michael, et al. (2017). Cancer Immunotherapy with Recombinant Poliovirus Induces IFN-Dominant Activation of Dendritic Cells and Tumor Antigen-Specific CTLs. Sci Transl Med, 31 pgs. PMID: 28931654. (link)

  28. Cheng, Liang, et al. (2017). Type I interferons suppress viral replication but contribute to T cell depletion and dysfunction during chronic HIV-1 infection. JCI Insight, 13 pgs. PMID: 28614789. (link)

  29. Luan, Liming, et al. (2017). Comparative Transcriptome Profiles of Human Blood in Response to the Toll-like Receptor 4 Ligands Lipopolysaccharide and Monophosphoryl Lipid A. Scientific Reports, 16 pgs. PMID: 28053314. (link)

  30. Gusella, Luca, et al. (2016). Prothymosin-α Variants Elicit Anti-HIV-1 Response via TLR4 Dependent and Independent Pathways. PLOS One, 17 pgs. PMID: 27310139. (link)

  31. GuhaSarkar, Dwijit, et al. (2016). Systemic AAV9-IFNβ gene delivery treats highly invasive glioblastoma. Neuro-Oncology, 11 pgs. PMID: 27194146. (link)

  32. Vanheule, Vincent, et al. (2016). Basic chemokine-derived glycosaminoglycan binding peptides exert antiviral properties against dengue virus serotype 2, herpes simplex virus-1 and respiratory syncytial virus. Biochemical Pharmacology, pgs. PMID: 26551597. (link)

  33. Chiappinelli, Katherine, et al. (2015). Inhibiting DNA methylation causes an interferon response in cancer via dsRNA including endogenous retroviruses. CellPress, 23 pgs. PMID: 26317466. (link)

  34. Dauletbaev, Nurlan, et al. (2015). Stimulation of the RIG-I/MAVS Pathway by Polyinosinic:Polycytidylic Acid Upregulates IFN-β in Airway Epithelial Cells with Minimal Costimulation of IL-8. Journal of Immunology, 14 pgs. PMID: 26283481. (link)

  35. Spengler, Jessica, et al. (2015). RIG-I Mediates an Antiviral Response to Crimean-Congo Hemorrhagic Fever Virus. JVI, 11 pgs. PMID: 26223644. (link)

  36. Eigenbrod, Tatjana, et al. (2015). TLR8 Senses Bacterial RNA in Human Monocytes and Plays a Nonredundant Role for Recognition of Streptococcus pyogenes. Journal of Immunology, 9 pgs. PMID: 26101323. (link)

  37. Bergstrom, Bjarte, et al. (2015). TLR8 Senses Staphylococcus aureus RNA in Human Primary Monocytes and Macrophages and Induces IFN-β Production via a TAK1-IKKβ-IRF5 Signaling Pathway. Journal of Immunology, 13 pgs. PMID: 26085680. (link)

  38. Olaisen, Camilla, et al. (2015). PCNA-interacting peptides reduce Akt phosphorylation and TLR-mediated cytokine secretion suggesting a role of PCNA in cellular signaling. Cellular Signaling, 10 pgs. PMID: 25797046. (link)

  39. White, Michael, et al. (2014). Apoptotic Caspases Suppress mtDNA-Induced STING-Mediated Type I IFN Production. CellPress, 27 pgs. PMID: 25525874. (link)

  40. Wolferstaetter, Michael, et al. (2014). Recombinant Modified Vaccinia Virus Ankara Generating Excess Early Double-Stranded RNA Transiently Activates Protein Kinase R and Triggers Enhanced Innate Immune Responses. JVI, 16 pgs. PMID: 25297997. (link)

  41. Asgari, et al. (2013). Clinical Study: Interferon Alpha Association with Neuromyelitis Optica. Clinical and Developmental Immunology, 7 pgs. PMID: 24348680. (link)

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  46. Wang, et al. (2012). Application of Secretary Luciferase Labeled Orthotopic Transplant Model of Hepatocellular Carcinoma to Evaluate Tumor Response to Interferon-beta Gene Therapy. Sheng Wu Gong Cheng Xue Bao. PMID: 23311138. (link)

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  55.  

 

Background Literature:

 

  1. Krause CD, Pestka S. Evolution of the Class 2 cytokines and receptors, and discovery of new friends and relatives. Pharmacol Ther. 2005 Jun;106(3):299-346.

  2. Weinstock-Guttman B, Ramanathan M, Zivadinov R. Interferon-beta treatment for relapsing multiple sclerosis. Expert Opin Biol Ther. 2008 Sep;8(9):1435-47.

  3. Lee MS, Kim YJ. Pattern-recognition receptor signaling initiated from extracellular, membrane, and cytoplasmic space. Mol Cells. 2007 Feb 28;23(1):1-10.

  4. Heim MH. The Jak-STAT pathway: cytokine signaling from the receptor to the nucleus. J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):75-120.

  5. Taniguchi T, Takaoka A. The interferon-alpha/beta system in antiviral responses: a multimodal machinery of gene regulation by the IRF family of transcription factors. Curr Opin Immunol. 2002 Feb;14(1):111-6.

  6. Lewerenz M, Mogensen KE, Uzé G. Shared receptor components but distinct complexes for alpha and beta interferons. J Mol Biol. 1998 Sep 25;282(3):585-99.

  7. Jaks E, Gavutis M, Uzé G, Martal J, Piehler J. Differential receptor subunit affinities of type I interferons govern differential signal activation. J Mol Biol. 2007 Feb 16;366 (2):525- 39.

  8. Marijanovic Z, Ragimbeau J, van der Heyden J, Uzé G, Pellegrini S. Comparable potency of IFNalpha2 and IFN beta on immediate JAK/STAT activation but differential downregulation of IFNAR2. Biochem J. 2007 Oct 1;407(1):141-51.

  9. Taniguchi T, Takaoka A. A weak signal for strong responses: interferon-alpha/beta revisited. Nat Rev Mol Cell Biol. 2001 May; 2(5):378-86.

  10. Aziz N, Nishanian P, Mitsuyasu R, Detels R, Fahey JL. Variables That Affect Assays for Plasma Cytokines and Soluble Activation Markers. Clin Diagn Lab Immunol. 1999 Jan: (6)1:89-95.

Documentation

Technical Data Sheet, Certificate of Analysis (CoA), Protocol, and Safety Data Sheet (SDS)
41415 CoA and Protocol A

41415 Certificate of Analysis and Protocol A

41415 CoA and Protocol B

41415 Certificate of Analysis and Protocol B

41415 SDS

41415 SDS

41415 TDS

41415 Technical Data Sheet

41415 Poster

Performance Characterization of a High Sensitivity Human Interferon Beta ELISA Kit in Healthy Serum, Patient Serum and Plasma Samples