Levels of IFN-Beta in 128 Normal Donor & 54 Multiple Sclerosis (MS) Patient Sera on & off IFN-Beta therapies
Representative Human IFN-Beta Standard Curve from 1.2-150 pg/ml using Protocol A
Representative Human IFN-Beta Standard Curve from 2.3-150 pg/ml using Protocol B
Correlation between IFN-Beta Standards prepared in Standard Diluent & Healthy Serum Pools
Standard Diluent mimics Healthy Donor Serum (HDS) in quantifying spiked IFN-Beta 1a
Human IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)
Catalog Number: 41415
This ELISA measures human IFN-Beta levels in serum, plasma, and tissue culture media (TCM) with 1.2 pg/ml LLOQ sensitivity. It is suitable for the measurement of IFN-Beta in autoimmune serum and of trademarked IFN-Beta 1a and IFN-Beta 1b therapeutic molecules in human serum. There is no detection inhibition by 3-log excess of soluble IFNAR2 protein.
Product Name: VeriKine-HS Human Interferon-Beta ELISA Kit (Serum, Plasma, TCM)
|Matrix Compatibility||Serum, Plasma, Tissue Culture Media (TCM)|
Protocol A: 1.2 - 150 pg/ml (for improved serum performance)
Protocol B: 2.3 - 150 pg/ml
Need more sensitivity? Check out our Sample Testing Services
Protocol A: 3 hours, 30 minutes
Protocol B: 3 hours
The VeriKine-HS Human IFN-Beta Serum ELISA Kit is developed to measure low or basal levels of Human IFN-Beta in autoimmune sera, normal sera/plasma, or tissue culture media (TCM) samples by sandwich ELISA. The LLOQ of this kit is 1.2 pg/ml. Basal levels of Type I IFNs, including IFN-Beta, are not fully understood. They are believed to be important for robust response to pathogens and may play additional roles in cellular homeostasis.
This assay is suitable for the measurement of trademarked therapeutic molecules in human serum samples. Researchers and clinical investigators examining a) the pharmacokinetics of IFN-Beta molecules, b) IFN-Beta as a biomarker, or c) IFN-Beta as a pharmacodynamic marker of TLR agent or other immune response modifier activity will find this immunoassay to be an essential laboratory tool.
*For a TCM-compatible ELISA, we recommend our Human IFN-Beta ELISA Kit, High Sensitivity (Cat. No. 41435-1).
|CVs and Spike Recovery||
Inter-Assay < 8%
Spike Recovery > 90% in Serum
No cross-reactivity detected with:
|Synonyms||Human Beta Interferon, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B|
|Expiration Date||12 months from the date of manufacture|
|Shipping Condition||Wet Ice|
- Pre-coated microtiter plate
- Plate Sealers
- Wash Solution Concentrate
- Human Interferon Beta 1a Standard, 100,000 pg/ml
- Standard Diluent
- Sample Buffer
- Antibody Concentrate
- HRP Conjugate Concentrate
- Diluent Additive III (for use in Protocol A)
- Assay Diluent
- TMB Substrate
- Stop Solution
Additional Materials Required (Not Provided)
- Microplate reader capable of reading an OD at a wavelength of 450 nm
- Variable volume microtiter pipettes
- Adjustable multichannel pipette (50-300 μl)
- Reagent reservoirs
- Wash bottle or plate washing system
- Distilled or deionized water
- Serological pipettes (1, 5, 10 or 25 ml)
- Disposable pipette tips (polypropylene)
- Plate shaker
Tech Info & Data
Interferon beta (IFN-Beta, IFNb) is part of the first wave of cytokine response in cells. Pathogen infection can result in the activation of interferon regulatory factor 3 (IRF3) which functions in trans to activate IFN-Beta gene transcription. IFN-Beta is biologically unique when compared to other interferons and studies have shown that IFN-Beta has overlapping and distinct gene expression patterns as compared to IFN-Alpha. It appears that IFN-Beta binds to the Type I IFN receptor with higher affinity than the other Type I IFNs and it may also regulate receptor internalization in a different manner. Additionally, IFN-Beta has long been known to inhibit viral replication as part of the body’s innate antiviral response and is used as a therapeutic for the treatment of Multiple Sclerosis (MS) and some tumors.
- Performance Characterization Of A High Sensitivity Human Interferon Beta ELISA Kit In Healthy Serum, Patient Serum And Plasma Samples (link)
- Validation of a Highly Sensitive Immunoassay for the Quantitation of Interferon Beta in Autoimmune Sera (link)
- Optimization and Validation of an ELISA kit for the Quantification of Four Interferon-Beta (IFN-β) Marketed Compounds in Human Serum (link)
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Technical Data Sheet, Certificate of Analysis (CoA), Protocol, and Safety Data Sheet (SDS)
Performance Characterization of a High Sensitivity Human Interferon Beta ELISA Kit in Healthy Serum, Patient Serum and Plasma Samples
VeriKine-HS Human IFN-Beta ELISA Product Flyer
Allows accurate Human IFN-β measurement to as low as 1.2 pg/ml in human serum, plasma, autoimmune disease sera or cell culture media