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Custom Production

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PBL's reagent development services offer customized production of cytokines, interferons, antibodies, and other proteins.

  • Recombinant protein production and purification (bacterial & mammalian)
  • Cytokine and antibody production and characterization
  • Hybridoma, polyclonal antibody, and cell line development
  • Protein and antibody labeling and biophysical characterization

Antibody Characterization Service

  • Affinity of protein-protein interactions; Antibody-antigen specificity and affinity: Real-time BLI-based (Bio-layer Interferometry) biophysical characterization of polyclonal and monoclonal antibody affinities (Kd) for antigen

  • Association and dissociation rates of protein-protein interactions: Quantification of  Kon and Koff rates for many antibody/antigen pairs underlying their dissociation constants

  • Epitope binning based upon antibody binding to non-overlapping epitopes

  • Antibody screening to reveal appropriate biophysical profiles for specific applications

With a wealth of knowledge on immunological interactions, we offer protein biophysical characterization services which can determine the kinetic characterization of protein-protein interactions. PBL offers biophysical characterization of antibody affinity for a ligand or the measurement of antigen:antibody Kon/Koff rates. To accelerate assay development, we also determine which antibodies bind to antigens in a sandwich format. Binning— determination of antibodies which bind to non-overlapping epitopes—is performed using multiple hybridoma clones.

The graph shows the association and dissociation of IFN to a surface-immobilized antibody as determined by real time Biolayer Interferometry (BLI) using varying concentrations of IFN. The raw data is shown in blue and the model response in red. IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by Bio-Layer Interferometry (BLI).

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