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Mouse Interferon Alpha All-Subtype Neutralizing Viral Protein

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Mouse Interferon Alpha All-Subtype Neutralizing Viral Protein

Catalog Number: 12185

  • C12R IFN Antagonist neutralizes All 14 Mouse IFN-Alpha subtypes, plus Mouse Limitin, Mouse IFN-Kappa, and Rat IFN-Alpha 1.

  • Also suitable for Western Blot.

$310.00

Pack Size
Product Info

Tested Applications

  • Neutralization
  • Western Blot (WB)

Specificity

Neutralizes:

  • All mouse and human IFN-Alpha subtypes
  • Human IFN-Beta, IFN-Omega
  • Rat IFN-Alpha 1
  • Mouse limitin

 

Does not neutralize the following when tested at ≥100X the typical neutralizing dose

  • Mouse IFN-Beta
  • Human IFN-Gamma, IFN-Lambda 1, IFN-Lambda 2, IFN-Lambda 3
  • Rat IFN-Alpha 14

Activity (EC50)

0.3 - 11.5 ng/ml (varies by subtype)

Specifications

FormulationSupplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight32 kDa
Purity> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
BioactivityMeasured by neutralization of interferon in a cytopathic effect inhibition assay.
StorageFor retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles

Tech Info & Data

Background

 

C12R is an interferon binding/blocking protein produced by many strains of Mousepox (Ectromelia) virus, (Smith and Alcami, J.Vir. 2002, 76:1124).  This protein blocks the activity of mouse IFN-Alpha but not Beta. It is an essential protein for viral infection (Xu et al. JEM 2008 205:981). 

Citations

1 Citation:

 

  1. Lee, S. et al., (2019), "A Secreted Viral Nonstructural Protein Determines Intestinal Norovirus Pathogenesis", Cell Host Microbe. 25(6):845, PMID: 31130511, DOI: 10.1016/j.chom.2019.04.005 (link)

Documentation

Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
12185-1 COA

12185-1 Certificate of Analysis

12185-2 CoA

12185-2 Certificate of Analysis

12185 SDS

12185 Safety Data Sheet