Anti-Human IFNAR2 Antibody, Clone MMHAR-2, neutralizing (MAb)

Home / Products / Antibodies / Monoclonal Antibodies (MAbs) / Anti-Human IFN MAbs / Anti-Human IFNAR2 Antibody, Clone MMHAR-2, neutralizing (MAb)
Product Features: 
  • Anti-IFNAR2 antibody (clone MMHAR-2) to human Interferon (Alpha, Beta and Omega) Receptor 2
  • Blocks the biological action of Human Type I Interferons
  • Suitable for use in Type I IFN neutralization, flow cytometry, immunohistochemistry, and immunoprecipitation

Full Product Name: Anti-Human Interferon Alpha/Beta Receptor Chain 2, Clone MMHAR-2 (MAb)

Ordering

Catalog No. Pack Size Price Quantity
21385-1

50 μg

 $495.00
$575.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Antigen Human interferon alpha/beta receptor chain 2 (Human IFNAR2)
Isotype IgG2A
Host Species Mouse
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured by neutralization of interferon in cytopathic effect inhibition assay.
Specificity Neutralizes human IFN-alpha receptor; interacts with extracellular domain; binds to human IFN-alpha receptor with high affinity; blocks biological action of Type I IFNs
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Antigen Synonyms None
Clone MMHAR-2

Tech Info / Data

Tested Applications:

  • Neutralization

Suggested Applications*:

  • Immunoprecipitation (1-5 μg/ml)
  • Immunohistochemistry (1 μg/ml)
  • Flow Cytometry (1-10 μg/ml)

*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL.

 

Background: 

The Type I interferon receptor is a heterodimer consisting of IFNAR1 and IFNAR2. The IFNAR2 subunit provides most of the binding affinity for IFN and IFNAR1 is required for optimum signaling.

Citations

44 Citations:

  1. Merindol, Natacha, et al. (2018). HIV-1 capsids from B27/B57+ elite controllers escape Mx2 but are targeted by TRIM5α, leading to the induction of an antiviral state. PLOS Pathogens, 22 pgs. PMID: 30419009. (link)

  2. Zavala, Kathryn, et al. (2018). Intrinsic activation of the vitamin D antimicrobial pathway by M. leprae infection is inhibited by type I IFN. PLOS Neglected Tropical Diseases, 15 pgs. PMID: 30300363. (link)

  3. Lucifora, et al. (2018). Direct antiviral properties of TLR ligands against HBV replication in immune-competent hepatocytes. Scientific Reports, 11 pgs. PMID: 29599452. (link)

  4. Scholte, Florine, et al. (2017). Crimean-Congo Hemorrhagic Fever Virus suppresses Innate Immune Responses via a Ubiquitin and ISG15 Specific Protease. Cell Reports, 24 pgs. PMID: 28877473. (link)

  5. Zhu, Zhe, et al. (2017). Zika virus has oncolytic activity against glioblastoma stem cells. JEM, 15 pgs. PMID: 28874392. (link)

  6. Diaz-Rodriguez, Yildian, et al. (2017). In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells. Cancer Immunology, Immunotherapy, 14 pgs. PMID: 28555259. (link)

  7. Wang, Ben, et al. (2017). A Conserved Residue, Tyrosine (Y) 84, in H5N1 Influenza A Virus NS1 Regulates IFN Signaling Responses to Enhance Viral Infection. Viruses, 21 pgs. PMID: 28498306. (link)

  8. Bowen, James, et al. (2017). Zika Virus Antagonizes Type I Interferon Responses during Infection of Human Dendritic Cells. PLOS Pathogens, 30 pgs. PMID: 28152048. (link)

  9. Schwartz, Jordan (2017). Elucidation of the Plasmacytoid Dendritic Cell Response to HIV-1. University of Toronto, 190 pgs. PMID: no PMID. (link)

  10. Ma, Tong-Cui, et al. (2016). Soybean-derived Bowman-Birk Inhibitor (BBI) Inhibits HIV Replication in Macrophages. Scientific Reports, 10 pgs. PMID: 27734899. (link)

  11. Cordeau, Martine, et al. (2016). Efficient Killing of High Risk Neuroblastoma Using Natural Killer Cells Activated by Plasmacytoid Dendritic Cells. PLOS One, 17 pgs. PMID: 27716850. (link)

  12. Yin, Wenwei, et al. (2016). Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production. Scientific Reports, 12 pgs. PMID: 27192960. (link)

  13. Saidi, Hela, et al. (2016). HMGB1 Is Involved in IFN-α Production and TRAIL Expression by HIV-1-Exposed Plasmacytoid Dendritic Cells: Impact of the Crosstalk with NK Cells. PLOS Pathogens, 30 pgs. PMID: 26871575. (link)

  14. Thomann, Sabrina, et al. (2015). Combined cytotoxic activity of an infectious, but non-replicative herpes simplex virus type 1 and plasmacytoid dendritic cells against tumour cells. Immunology, 12 pgs. PMID: 26194553. (link)

  15. Kyriakidis, et al. (2014). TLR3 Stimulation Promotes Ro52/TRIM21 Synthesis and Nuclear Redistribution in Salivary Gland Epithelial Cells, Partially via Type I Interferon Pathway. Clinical & Experimental Immunology, 13 pgs. PMID: 25098814. (link)

  16. Bowers, et al. (2014). Immune Suppression by Neutrophils in HIV-1 Infection: Role of PD-L1/PD-1 Pathway. PLOS One, 9 pgs. PMID: 24626392. (link)

  17. Tsugawa, et al. (2014). Critical Role of Interferon-alpha Constitutively Produced in Human Hepatocytes in Response to RNA Virus Infection. PLOS One, 9 pgs. PMID: 24587086. (link)

  18. Schijf, et al. (2013). Respiratory Syncytical Virus Induced Type I IFN Production by pDC is Regulated by RSV-Infected Airway Epithelial Cells, RSV-Exposed Monocytes and Virus Specific Antibodies. PLOS One, 18 pgs. PMID: 24303065. (link)

  19. Jing, et al. (2014). Intracellular Poly(I:C) Initiated Gastric Adenocarcinoma Cell Apoptosis and Subsequently Ammeliorated NK Cell Functions. Journal of Interferon & Cytokine Research. PMID: 24032591. (link)

  20. Rodrigues, et al. (2013). Candida albicans Delays HIV-1 Replication in Macrophages. PLOS One, 11 pgs. PMID: 24009706. (link)

  21. Kuri, et al. (2013). Influenza A Virus-Mediated Priming Enhances Cytokine Secretion by Human Dendritic Cells Infected with Streptococcus pneumoniae. Cellular Microbiology, 16 pgs. PMID: 23421931. (link)

  22. Dickensheets, et al. (2013). Interferon-lambda Induces Signal Transduction and Gene Expression in Human Hepatocytes, but Not in Lymphocytes or Monocytes. JLB. PMID: 23258595. (link)

  23. Patel, et al. (2012). Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. PLOS One, 11 pgs. PMID: 22558189. (link)

  24. Rodrigues, et al. (2011). Epithelial Cells Activate Plasmacytoid Dendritic Cells Improving Their Anti-HIV Activity. PLOS One, 13 pgs. PMID: 22163327. (link)

  25. Pascutti, et al. (2011). Interplay Between Modified Vaccinia Virus Ankara and Dendritic Cells: Phenotypic and Functional Maturation of Bystander Dendritic Cells. JVI, 14 pgs. PMID: 21411535. (link)

  26. Inglefield, et al. (2008). TLR7 agonist 852A inhibition of tumor cell proliferation is dependent on plasmacytoid dendritic cells and type I IFN. Journal of Interferon & Cytokine Research. PMID: 18439103. (link)

  27. Moll, et al. (2008). Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells. Journal of Immunology, 8 pgs. PMID: 18390705. (link)

  28. Davis, Ann, et al. (2008). A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells. Journal of Immunology, 11 pgs. PMID: 19050236. (link)

  29. Marijanovic, Zrinka, et al. (2007). Comparable potency of IFNα2 and IFNβ on immediate JAK/STAT activation but differential down-regulation of IFNAR2. Biochemical Journal, 11 pgs. PMID: 17627610. (link)

  30. Hasan, Uzma, et al. (2007). Cell proliferation and survival induced by Toll-like receptors is antagonized by type I IFNs. PNAS, 6 pgs. PMID: 17463087. (link)

  31. Conti, Laura, et al. (2007). In the absence of IGF-1 signaling, IFN-gamma suppresses human malignant T-cell growth. Blood Journal, 10 pgs. PMID: 17148586. (link)

  32. van Boxel-Dezaire, Annette, et al. (2006). Complex modulation of cell type-specific signaling in response to type I interferons. Immunity, 12 pgs. PMID: 16979568. (link)

  33. Best, et al. (2005). Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist. JVI, 12 pgs. PMID: 16188985. (link)

  34. Bendriss-Vermare, Nathalie, et al. (2005). Virus overrides the propensity of human CD40L-activated plasmacytoid dendritic cells to produce Th2 mediators through synergistic induction of IFN-{gamma} and Th1 chemokine production. JLB, 13 pgs. PMID: 16081597. (link)

  35. Nestle, Frank, et al. (2005). Plasmacytoid predendritic cells initiate psoriasis through interferon-α production. JEM, 9 pgs. PMID: 15998792. (link)

  36. Gautier, Gregory, et al. (2005). A type I interferon autocrine–paracrine loop is involved in Toll-like receptor-induced interleukin-12p70 secretion by dendritic cells. JEM, 12 pgs. PMID: 15851485. (link)

  37. Bekeredjian-Ding, Isabelle, et al. (2005). Plasmacytoid dendritic cells control TLR7 sensitivity of naive B cells via type I IFN. Journal of Immunology, 10 pgs. PMID: 15778362. (link)

  38. Gustincich, Stefano, et al. (2004). Gene discovery in genetically labeled single dopaminergic neurons of the retina. PNAS, 6 pgs. PMID: 15047890. (link)

  39. Kato, Atsushi, et al. (2003). Interferon-α/β receptor-mediated selective induction of a gene cluster by CpG oligodeoxynucleotide 2006. BMC Immunology, 10 pgs. PMID: 12887736. (link)

  40. Dondi, Elisabetta, et al. (2003). Down-modulation of responses to type I IFN upon T cell activation. Journal of Immunology, 9 pgs. PMID: 12517937. (link)

  41. Zou, Weiping, et al. (2001). Reciprocal regulation of plasmacytoid dendritic cells and monocytes during viral infection. European Journal of Immunology, 7 pgs. PMID: 11745405. (link)

  42. Dondi, Elisabetta, et al. (2001). Down-modulation of type 1 interferon responses by receptor cross-competition for a shared Jak kinase. JBC, 10 pgs. PMID: 11602573. (link)

  43. Schlender, Joerg, et al. (2000). Bovine Respiratory Syncytial Virus Nonstructural Proteins NS1 and NS2 Cooperatively Antagonize Alpha/Beta Interferon-Induced Antiviral Response. JVI, 9 pgs. PMID: 10954520. (link)

  44. Colamonici, Oscar, et al. (1993). Identification of a Novel Subunit of the Type I Interferon Receptor Localized to Human Chromosome 21. JBC, 5 pgs. PMID: 8496154. (link)

    Reference:

    1. Colamonici, Oscar, et al. (1993). Identification of a Novel Subunit of the Type I Interferon Receptor Localized to Human Chromosome 21. JBC, 5 pgs. PMID: 8496154. (link)

    Documentation

    Neutralization Effect of PBL Anti-Human IFNAR2 Monoclonal Antibody (21385) on Human IFN Alpha 2a (11100) and IFN Beta (11415) Activities in an A549 Cell/EMCV CPE Assay

     

    Image depicting PBL Anti-Human IFNAR2 Monoclonal's neutralizing effect on Human IFN Alpha and IFN Beta protection of A549 cells from the cytopathic effect of EMCV infection

    Related Products

    Catalog No. Description Pack Size Price Quantity
    21370-1 Mouse Monoclonal Antibody against Human Interferon Alpha/Beta Receptor 1, Clone MMHAR-3 (MAb)  new 

    50 µg

    		 $295.00
    21385-3 PE Conjugated Anti-Human Interferon Alpha/Beta Receptor Chain 2 (IFNAR2), Clone MMHAR-2 (MAb)

    50 tests

    		 $495.00

    Product Selector

    Find your product of interest by searching for category, species, molecule and/or application.

    Ahead of the Curve

    Visit our blog to read about recent developments in the fields of interferon, cytokine and biomarker research!

    Email Subscription

    Sign up for our mailing list to be kept up-to-date on the latest promotions, company and product news.

    Go to top