Anti-Human IFNAR2 Antibody, Clone MMHAR-2, neutralizing (MAb)

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Product Features: 
  • Anti-IFNAR2 antibody (clone MMHAR-2) to human Interferon (Alpha, Beta and Omega) Receptor 2
  • Blocks the biological action of Human Type I Interferons
  • Suitable for use in Type I IFN neutralization, flow cytometry, immunohistochemistry, and immunoprecipitation

Full Product Name: Anti-Human Interferon Alpha/Beta Receptor Chain 2, Clone MMHAR-2 (MAb)

Ordering

Catalog No. Pack Size Price Quantity
21385-1

50 μg

 $495.00
$575.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Antigen Human interferon alpha/beta receptor chain 2 (Human IFNAR2)
Isotype IgG2A
Host Species Mouse
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured by neutralization of interferon in cytopathic effect inhibition assay.
Specificity Neutralizes human IFN-alpha receptor; interacts with extracellular domain; binds to human IFN-alpha receptor with high affinity; blocks biological action of Type I IFNs
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Antigen Synonyms None
Clone MMHAR-2

Tech Info / Data

Tested Applications:

  • Neutralization

Suggested Applications*:

  • Immunoprecipitation (1-5 μg/ml)
  • Immunohistochemistry (1 μg/ml)
  • Flow Cytometry (1-10 μg/ml)

*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL.

 

Background: 

The Type I interferon receptor is a heterodimer consisting of IFNAR1 and IFNAR2. The IFNAR2 subunit provides most of the binding affinity for IFN and IFNAR1 is required for optimum signaling.

Citations

Citations:

  1. Immune Suppression by Neutrophils in HIV-1 Infection: Role of PD-L1/PD-1 Pathway. Bowers et al., 2014, PLoS One, 10(3):e1003993 (link)

  2. TLR3 stimulation promotes Ro52/TRIM21 synthesis and nuclear redistribution in salivary gland epithelial cells, partially via type I interferon pathway. Kyriakidis et al., 2014, Clin.Expr. Immunol., doi:10.1111/cei.12432 (link)

  3. Critical Role of Interferon-α Constitutively Produced in Human Hepatocytes in Response to RNA Virus Infection. Tsugawa et al., 2014, PLoS One, 9(2): e89869 (link)

  4. Intracellular Poly(I:C) Initiated Gastric Adenocarinoma Cell Apoptosis and Subsequently Ammeliorated NK cell Functions. Jing et al., 2014, J. Interferon & Cytokine Research, 34(1):52 (link)

  5. Respiratory Syncytical Virus Induced Type I IFN Production by pDC Is Regulated by RSV-Infected Airway Epithelial Cells, RSV-Exposed Monocytes and Virus Specific Antibodies. Schijf et al., 2013, PLoS One, 8(11):e81695 (link)

  6. Candida albicans Delaays HIV-1 Replication in Macrophages. Rodriguse et al., 2013, PLoS One, 8(8):e72814 (link)

  7. Influenza A virus-mediated priming enhances cytokine secretion by human dendritic cells infected with Streptococcus pneumoniae. Kuri et al., 2013, Cellular Microbiology, 15(8):1385 (link)

  8. Interferon-lambda (IFN-λ) induces signal transduction and gene expression in human hepatocytes, but not in lymphocytes or monocytes. Dickensheets et al., 2013, J. Leukoc. Biol., 93:377 (link)

  9. Interplay between Modified Vaccinia Virus Ankara and Dendritic Cells: Phenotypic and Functional Maturation of Bystander Dendritic Cells. Pascutti et al., 2011, J. Virol., 85(11):5532 (link)

  10. Epithelial Cells Activate Plasmacytoid Dendritic Cells Improving Their Anti-HIV Activity. Rodrigues et al., 2011, PLoS One, 6(12):e28709 (link)

 

References

  1. Colamonici, O.R. et al. (1993) J. Biol. Chem. 268: 10895-10899.

Documentation

Neutralization Effect of PBL Anti-Human IFNAR2 Monoclonal Antibody (21385) on Human IFN Alpha 2a (11100) and IFN Beta (11415) Activities in an A549 Cell/EMCV CPE Assay

 

Image depicting PBL Anti-Human IFNAR2 Monoclonal's neutralizing effect on Human IFN Alpha and IFN Beta protection of A549 cells from the cytopathic effect of EMCV infection

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