Anti-Human IFNAR2 Antibody, Clone MMHAR-2, neutralizing (MAb)

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Product Features: 
  • Anti-IFNAR2 antibody (clone MMHAR-2) to human Interferon (Alpha, Beta and Omega) Receptor 2
  • IFN Receptor antibody blocks the biological action of Human Type I Interferons
  • Suitable for use in Type I IFN neutralization, flow cytometry, immunohistochemistry, and immunoprecipitation

Full Product Name: Anti-Human Interferon Alpha/Beta Receptor Chain 2, Clone MMHAR-2 (MAb)

Ordering

Catalog No. Pack Size Price Quantity
21385-1

50 μg

 $510.00
$590.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Antigen Human interferon alpha/beta receptor chain 2 (Human IFNAR2)
Isotype IgG2A
Host Species Mouse
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured by neutralization of interferon in cytopathic effect inhibition assay.
Specificity Neutralizes human IFN-alpha receptor; interacts with extracellular domain; binds to human IFN-alpha receptor with high affinity; blocks biological action of Type I IFNs
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Antigen Synonyms None
Clone MMHAR-2

Tech Info / Data

Tested Applications:

  • Neutralization

Suggested Applications*:

  • Immunoprecipitation (1-5 μg/ml)
  • Immunohistochemistry (1 μg/ml)
  • Flow Cytometry (1-10 μg/ml)

*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL.

 

Background: 

The Type I interferon receptor is a heterodimer consisting of IFNAR1 and IFNAR2. The IFNAR2 subunit provides most of the binding affinity for IFN and IFNAR1 is required for optimum signaling.

Citations

51 Citations:

  1. Yu, C. et al., (2022), "ID2 inhibits innate antiviral immunity by blocking TBK1- and IKKε-induced activation of IRF3", Science Signaling, 15:715, DOI: 10.1126/scisignal.abh0068 (link)
  2. Facchini, F.A., et al. (2021). Synthetic Glycolipids as Molecular Vaccine Adjuvants: Mechanism of Action in Human Cells and In Vivo Activity, J. Med. Chem., DOI:10.1021/acs.jmedchem.1c00896 (link)

  3. Sabree, SA., et al. (2020).  Direct and indirect immune effecrs of CMP-001, a virus-like particle containing a TLR-9 agnoist, Journal of ImmunoTherapy of Cancer, 9:e002484, DOI: 10.1136/jitc-2021-002484. (link)

  4. Colpitts, Che, et al. (2020). Hepatitis C virus exploits cyclophilin A to evade PKR. eLife, 25 pgs. PMID: 32539931. (link)

  5. Kullaya, Vesla Ignace (2019). The interplay between platelets, infections, and inflammation. Radboud University Nijmegen, 181 pgs. PMID: no PMID. (link)

  6. Merindol, Natacha, et al. (2018). HIV-1 capsids from B27/B57+ elite controllers escape Mx2 but are targeted by TRIM5α, leading to the induction of an antiviral state. PLOS Pathogens, 22 pgs. PMID: 30419009. (link)

  7. Zavala, Kathryn, et al. (2018). Intrinsic activation of the vitamin D antimicrobial pathway by M. leprae infection is inhibited by type I IFN. PLOS Neglected Tropical Diseases, 15 pgs. PMID: 30300363. (link)

  8. Lucifora, et al. (2018). Direct antiviral properties of TLR ligands against HBV replication in immune-competent hepatocytes. Scientific Reports, 11 pgs. PMID: 29599452. (link)

  9. Scholte, Florine, et al. (2017). Crimean-Congo Hemorrhagic Fever Virus suppresses Innate Immune Responses via a Ubiquitin and ISG15 Specific Protease. Cell Reports, 24 pgs. PMID: 28877473. (link)

  10. Zhu, Zhe, et al. (2017). Zika virus has oncolytic activity against glioblastoma stem cells. JEM, 15 pgs. PMID: 28874392. (link)

  11. Diaz-Rodriguez, Yildian, et al. (2017). In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells. Cancer Immunology, Immunotherapy, 14 pgs. PMID: 28555259. (link)

  12. Wang, Ben, et al. (2017). A Conserved Residue, Tyrosine (Y) 84, in H5N1 Influenza A Virus NS1 Regulates IFN Signaling Responses to Enhance Viral Infection. Viruses, 21 pgs. PMID: 28498306. (link)

  13. Bowen, James, et al. (2017). Zika Virus Antagonizes Type I Interferon Responses during Infection of Human Dendritic Cells. PLOS Pathogens, 30 pgs. PMID: 28152048. (link)

  14. Schwartz, Jordan (2017). Elucidation of the Plasmacytoid Dendritic Cell Response to HIV-1. University of Toronto, 190 pgs. PMID: no PMID. (link)

  15. Ma, Tong-Cui, et al. (2016). Soybean-derived Bowman-Birk Inhibitor (BBI) Inhibits HIV Replication in Macrophages. Scientific Reports, 10 pgs. PMID: 27734899. (link)

  16. Cordeau, Martine, et al. (2016). Efficient Killing of High Risk Neuroblastoma Using Natural Killer Cells Activated by Plasmacytoid Dendritic Cells. PLOS One, 17 pgs. PMID: 27716850. (link)

  17. Yin, Wenwei, et al. (2016). Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production. Scientific Reports, 12 pgs. PMID: 27192960. (link)

  18. Saidi, Hela, et al. (2016). HMGB1 Is Involved in IFN-α Production and TRAIL Expression by HIV-1-Exposed Plasmacytoid Dendritic Cells: Impact of the Crosstalk with NK Cells. PLOS Pathogens, 30 pgs. PMID: 26871575. (link)

  19. Thomann, Sabrina, et al. (2015). Combined cytotoxic activity of an infectious, but non-replicative herpes simplex virus type 1 and plasmacytoid dendritic cells against tumour cells. Immunology, 12 pgs. PMID: 26194553. (link)

  20. Kyriakidis, et al. (2014). TLR3 Stimulation Promotes Ro52/TRIM21 Synthesis and Nuclear Redistribution in Salivary Gland Epithelial Cells, Partially via Type I Interferon Pathway. Clinical & Experimental Immunology, 13 pgs. PMID: 25098814. (link)

  21. Bowers, et al. (2014). Immune Suppression by Neutrophils in HIV-1 Infection: Role of PD-L1/PD-1 Pathway. PLOS One, 9 pgs. PMID: 24626392. (link)

  22. Tsugawa, et al. (2014). Critical Role of Interferon-alpha Constitutively Produced in Human Hepatocytes in Response to RNA Virus Infection. PLOS One, 9 pgs. PMID: 24587086. (link)

  23. Schijf, et al. (2013). Respiratory Syncytical Virus Induced Type I IFN Production by pDC is Regulated by RSV-Infected Airway Epithelial Cells, RSV-Exposed Monocytes and Virus Specific Antibodies. PLOS One, 18 pgs. PMID: 24303065. (link)

  24. Jing, et al. (2014). Intracellular Poly(I:C) Initiated Gastric Adenocarcinoma Cell Apoptosis and Subsequently Ammeliorated NK Cell Functions. Journal of Interferon & Cytokine Research. PMID: 24032591. (link)

  25. Rodrigues, et al. (2013). Candida albicans Delays HIV-1 Replication in Macrophages. PLOS One, 11 pgs. PMID: 24009706. (link)

  26. Kuri, et al. (2013). Influenza A Virus-Mediated Priming Enhances Cytokine Secretion by Human Dendritic Cells Infected with Streptococcus pneumoniae. Cellular Microbiology, 16 pgs. PMID: 23421931. (link)

  27. Dickensheets, et al. (2013). Interferon-lambda Induces Signal Transduction and Gene Expression in Human Hepatocytes, but Not in Lymphocytes or Monocytes. JLB. PMID: 23258595. (link)

  28. Patel, et al. (2012). Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. PLOS One, 11 pgs. PMID: 22558189. (link)

  29. Rodrigues, et al. (2011). Epithelial Cells Activate Plasmacytoid Dendritic Cells Improving Their Anti-HIV Activity. PLOS One, 13 pgs. PMID: 22163327. (link)

  30. Pascutti, et al. (2011). Interplay Between Modified Vaccinia Virus Ankara and Dendritic Cells: Phenotypic and Functional Maturation of Bystander Dendritic Cells. JVI, 14 pgs. PMID: 21411535. (link)

  31. Inglefield, et al. (2008). TLR7 agonist 852A inhibition of tumor cell proliferation is dependent on plasmacytoid dendritic cells and type I IFN. Journal of Interferon & Cytokine Research. PMID: 18439103. (link)

  32. Moll, et al. (2008). Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells. Journal of Immunology, 8 pgs. PMID: 18390705. (link)

  33. Davis, Ann, et al. (2008). A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells. Journal of Immunology, 11 pgs. PMID: 19050236. (link)

  34. Marijanovic, Zrinka, et al. (2007). Comparable potency of IFNα2 and IFNβ on immediate JAK/STAT activation but differential down-regulation of IFNAR2. Biochemical Journal, 11 pgs. PMID: 17627610. (link)

  35. Hasan, Uzma, et al. (2007). Cell proliferation and survival induced by Toll-like receptors is antagonized by type I IFNs. PNAS, 6 pgs. PMID: 17463087. (link)

  36. Conti, Laura, et al. (2007). In the absence of IGF-1 signaling, IFN-gamma suppresses human malignant T-cell growth. Blood Journal, 10 pgs. PMID: 17148586. (link)

  37. van Boxel-Dezaire, Annette, et al. (2006). Complex modulation of cell type-specific signaling in response to type I interferons. Immunity, 12 pgs. PMID: 16979568. (link)

  38. Best, et al. (2005). Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist. JVI, 12 pgs. PMID: 16188985. (link)

  39. Bendriss-Vermare, Nathalie, et al. (2005). Virus overrides the propensity of human CD40L-activated plasmacytoid dendritic cells to produce Th2 mediators through synergistic induction of IFN-{gamma} and Th1 chemokine production. JLB, 13 pgs. PMID: 16081597. (link)

  40. Nestle, Frank, et al. (2005). Plasmacytoid predendritic cells initiate psoriasis through interferon-α production. JEM, 9 pgs. PMID: 15998792. (link)

  41. Gautier, Gregory, et al. (2005). A type I interferon autocrine–paracrine loop is involved in Toll-like receptor-induced interleukin-12p70 secretion by dendritic cells. JEM, 12 pgs. PMID: 15851485. (link)

  42. Bekeredjian-Ding, Isabelle, et al. (2005). Plasmacytoid dendritic cells control TLR7 sensitivity of naive B cells via type I IFN. Journal of Immunology, 10 pgs. PMID: 15778362. (link)

  43. Gustincich, Stefano, et al. (2004). Gene discovery in genetically labeled single dopaminergic neurons of the retina. PNAS, 6 pgs. PMID: 15047890. (link)

  44. Kato, Atsushi, et al. (2003). Interferon-α/β receptor-mediated selective induction of a gene cluster by CpG oligodeoxynucleotide 2006. BMC Immunology, 10 pgs. PMID: 12887736. (link)

  45. Dondi, Elisabetta, et al. (2003). Down-modulation of responses to type I IFN upon T cell activation. Journal of Immunology, 9 pgs. PMID: 12517937. (link)

  46. Zou, Weiping, et al. (2001). Reciprocal regulation of plasmacytoid dendritic cells and monocytes during viral infection. European Journal of Immunology, 7 pgs. PMID: 11745405. (link)

  47. Dondi, Elisabetta, et al. (2001). Down-modulation of type 1 interferon responses by receptor cross-competition for a shared Jak kinase. JBC, 10 pgs. PMID: 11602573. (link)

  48. Schlender, Joerg, et al. (2000). Bovine Respiratory Syncytial Virus Nonstructural Proteins NS1 and NS2 Cooperatively Antagonize Alpha/Beta Interferon-Induced Antiviral Response. JVI, 9 pgs. PMID: 10954520. (link)

  49. Colamonici, Oscar, et al. (1993). Identification of a Novel Subunit of the Type I Interferon Receptor Localized to Human Chromosome 21. JBC, 5 pgs. PMID: 8496154. (link)

  50. Muendlein H, et al. (2020) Constitutive Interferon Attenuates RIPK1/3-Mediated Cytokine Translation. Cell Reports , 14 pgs. (link)

  51. Boussier J, et al. (2020) Chikungunya Virus Superinfection Exclusion is Mediated by a Block in Viral Replication and Does Not Rely on Non-Structural Protein 2. PLOS ONE., (link)

Reference:

  1. Colamonici, Oscar, et al. (1993). Identification of a Novel Subunit of the Type I Interferon Receptor Localized to Human Chromosome 21. JBC, 5 pgs. PMID: 8496154. (link)

Documentation

Neutralization Effect of PBL Anti-Human IFNAR2 Monoclonal Antibody (21385) on Human IFN Alpha 2a (11100) and IFN Beta (11415) Activities in an A549 Cell/EMCV CPE Assay

 

Image depicting PBL Anti-Human IFNAR2 Monoclonal's neutralizing effect on Human IFN Alpha and IFN Beta protection of A549 cells from the cytopathic effect of EMCV infection

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