Anti-Human IFNAR2 Antibody, Clone MMHAR-2, neutralizing (MAb)

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Product Features: 
  • Anti-IFNAR2 antibody (clone MMHAR-2) to human Interferon (Alpha, Beta and Omega) Receptor 2
  • Blocks the biological action of Human Type I Interferons
  • Suitable for use in Type I IFN neutralization, flow cytometry, immunohistochemistry, and immunoprecipitation

Full Product Name: Anti-Human Interferon Alpha/Beta Receptor Chain 2, Clone MMHAR-2 (MAb)

Ordering

Catalog No. Pack Size Price Quantity
21385-1

50 μg

 $495.00
$575.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Antigen Human interferon alpha/beta receptor chain 2 (Human IFNAR2)
Isotype IgG2A
Host Species Mouse
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured by neutralization of interferon in cytopathic effect inhibition assay.
Specificity Neutralizes human IFN-alpha receptor; interacts with extracellular domain; binds to human IFN-alpha receptor with high affinity; blocks biological action of Type I IFNs
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Antigen Synonyms None
Clone MMHAR-2

Tech Info / Data

Tested Applications:

  • Neutralization

Suggested Applications*:

  • Immunoprecipitation (1-5 μg/ml)
  • Immunohistochemistry (1 μg/ml)
  • Flow Cytometry (1-10 μg/ml)

*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL.

 

Background: 

The Type I interferon receptor is a heterodimer consisting of IFNAR1 and IFNAR2. The IFNAR2 subunit provides most of the binding affinity for IFN and IFNAR1 is required for optimum signaling.

Citations

45 Citations:

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  43. Dondi, Elisabetta, et al. (2001). Down-modulation of type 1 interferon responses by receptor cross-competition for a shared Jak kinase. JBC, 10 pgs. PMID: 11602573. (link)

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Reference:

  1. Colamonici, Oscar, et al. (1993). Identification of a Novel Subunit of the Type I Interferon Receptor Localized to Human Chromosome 21. JBC, 5 pgs. PMID: 8496154. (link)

Documentation

Neutralization Effect of PBL Anti-Human IFNAR2 Monoclonal Antibody (21385) on Human IFN Alpha 2a (11100) and IFN Beta (11415) Activities in an A549 Cell/EMCV CPE Assay

 

Image depicting PBL Anti-Human IFNAR2 Monoclonal's neutralizing effect on Human IFN Alpha and IFN Beta protection of A549 cells from the cytopathic effect of EMCV infection

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