Mouse IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

Product Features: 
  • Sensitive global detection of all 14 mouse IFN alpha in serum, plasma, and tissue culture media
  • Quantification of mouse IFN-α as low as 2.38 pg/ml
  • Rapid ELISA provides total interferon alpha readout in ~ 2 hrs
  • High data reproducibility with less than 10% CVs

Full Product Name: VeriKine-HS Mouse Interferon Alpha All Subtype ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
42115-1

1 x 96-well plate

$595.00
$685.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 2.38 - 152 pg/ml
Speed: Typical Incubation time, 1 hour, 54 minutes
Specificity: Recognizes all 14 Mouse Interferon Alpha subtypes
No cross-reactivity against mouse IFN-β, mouse IFN-γ, rat IFN-α1, or IFN-α14; very weak cross-reactivity with human IFN-α14.
Synonyms: IFN alpha, IFN-a, Multisubtype Interferon Alpha, alpha interferon, Type I interferon

 

Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 80% in Serum

 

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice


Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Alpha 4 Standard, 10,000 pg/ml
  • Sample Diluent
  • Antibody Concentrate
  • Antibody Diluent
  • HRP Conjugate Concentrate
  • HRP Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Image of mouse IFN alpha standard concentration curve from 2.38 pg/ml to 152 pg/ml plotted against mean absorbance using PBL's Mouse Interferon Alpha All-Subtype ELISA kit
Image of a quick reference guide for the procedure of PBL's Mouse IFN Alpha All-Subtype ELISA (42115)
Background: 

The VeriKine-HS Mouse IFN-α ELISA kit has been developed to quantitate low levels of IFN-α in mouse serum, plasma and cell/tissue culture media (10% FBS) using a sandwich immunoassay. The kit contains an anti-mouse IFN-α detection antibody conjugated to biotin and quantified by streptavidin horseradish peroxidase (HRP) using tetramethyl-benzidine (TMB) as a substrate. The kit standard is mouse IFN-α 4 protein, and typical standard curves are included with the procedure. It is important to run the standard curve in the same matrix as one’s samples unless one has demonstrated that the matrix does not significantly affect the signal.


Interferons (IFNs) are an extensive set of cytokines exhibiting pleitropic activities and playing major roles in both innate and adaptive immunity. The Type I IFNs genes consist of multiple IFN-α genes, at least one IFN-β gene in most vertebrates, and a few other family members including limitin in the mouse and IFN-ω and -ε in mouse and human. In the mouse, fourteen (14) IFN-α genes have been cloned which exhibit at least 75% identity at the protein level. In the therapeutic setting, IFN-α is used to treat viral diseases and certain cancers, and is therefore frequently studied in mouse and other animal models of these diseases. Several lines of evidence indicate that IFN-α proteins may be involved in the development of specific autoimmune diseases, and that treatment with IFN-α blocking antibodies may comprise a useful therapeutic strategy.

 

Expression of interferon genes (and therefore, proteins) is regulated by a group of interferon regulatory factors, i.e., the IRF family of proteins. Cellular levels of IRF3 and IRF7 regulate the expression of individual IFN-α subtypes with most IFN-α subtype genes requiring IRF7 for expression. IRF7 protein is either constitutively expressed or inducible, depending on cell type. In certain mouse cell lines, IRF7 expression is induced in particular by at least IFN-β and IFN-α4 which then leads to expression of the other IFN-α subtype genes. Additionally, murine IFN-β may be requisite for fibroblast synthesis of IFN-α but may not be requisite for other cells’ synthesis of IFN-α. The IRF3/IRF7 signaling cascade is a focal point for initial and progressive cellular responses to pathogens where hundreds of genes are subsequently regulated in a temporally coordinated cascade.

 

Plasmacytoid dendritic cells (PDCs) are highly efficient producers of IFN-α, but a wide variety of cells can also produce lower levels of these protein subtypes. The particular IFN-α subtype genes that are expressed appear to be somewhat cell and stimulus specific and, based on the results published from several gene induction studies, the time courses of induction of the individual protein subtypes are expected to differ markedly.

Citations

Levels of Endogenous Mouse IFN Alpha

Levels of Endogenous Mouse IFN Alpha using 42115-1 ELISA

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