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Full Product Name: VeriKine-HS Mouse Interferon Alpha All Subtype ELISA Kit
Catalog No. | Pack Size | Price | Quantity |
---|---|---|---|
42115-1 |
1 x 96-well plate |
$605.00 $695.00 |
Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 2.38 - 152 pg/ml
Speed: Typical Incubation time, 1 hour, 54 minutes
Specificity: Recognizes all 14 Mouse Interferon Alpha subtypes
No cross-reactivity against mouse IFN-β, mouse IFN-γ, rat IFN-α1, or IFN-α14; very weak cross-reactivity with human IFN-α14.
Synonyms: IFN alpha, IFN-a, Multisubtype Interferon Alpha, alpha interferon, Type I interferon
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 80% in Serum
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice
Materials Provided:
Detailed Related Product Information:
The VeriKine-HS Mouse IFN-α ELISA kit has been developed to quantitate low levels of IFN-α in mouse serum, plasma and cell/tissue culture media (10% FBS) using a sandwich immunoassay. The kit contains an anti-mouse IFN-α detection antibody conjugated to biotin and quantified by streptavidin horseradish peroxidase (HRP) using tetramethyl-benzidine (TMB) as a substrate. The kit standard is mouse IFN-α 4 protein, and typical standard curves are included with the procedure. It is important to run the standard curve in the same matrix as one’s samples unless one has demonstrated that the matrix does not significantly affect the signal.
Interferons (IFNs) are an extensive set of cytokines exhibiting pleitropic activities and playing major roles in both innate and adaptive immunity. The Type I IFNs genes consist of multiple IFN-α genes, at least one IFN-β gene in most vertebrates, and a few other family members including limitin in the mouse and IFN-ω and -ε in mouse and human. In the mouse, fourteen (14) IFN-α genes have been cloned which exhibit at least 75% identity at the protein level. In the therapeutic setting, IFN-α is used to treat viral diseases and certain cancers, and is therefore frequently studied in mouse and other animal models of these diseases. Several lines of evidence indicate that IFN-α proteins may be involved in the development of specific autoimmune diseases, and that treatment with IFN-α blocking antibodies may comprise a useful therapeutic strategy.
Expression of interferon genes (and therefore, proteins) is regulated by a group of interferon regulatory factors, i.e., the IRF family of proteins. Cellular levels of IRF3 and IRF7 regulate the expression of individual IFN-α subtypes with most IFN-α subtype genes requiring IRF7 for expression. IRF7 protein is either constitutively expressed or inducible, depending on cell type. In certain mouse cell lines, IRF7 expression is induced in particular by at least IFN-β and IFN-α4 which then leads to expression of the other IFN-α subtype genes. Additionally, murine IFN-β may be requisite for fibroblast synthesis of IFN-α but may not be requisite for other cells’ synthesis of IFN-α. The IRF3/IRF7 signaling cascade is a focal point for initial and progressive cellular responses to pathogens where hundreds of genes are subsequently regulated in a temporally coordinated cascade.
Plasmacytoid dendritic cells (PDCs) are highly efficient producers of IFN-α, but a wide variety of cells can also produce lower levels of these protein subtypes. The particular IFN-α subtype genes that are expressed appear to be somewhat cell and stimulus specific and, based on the results published from several gene induction studies, the time courses of induction of the individual protein subtypes are expected to differ markedly.
6 Citations:
Catalog No. | Description | Pack Size | Price | Quantity |
---|---|---|---|---|
12115-1 |
Mouse Interferon Alpha 4, mammalian |
1 x 105 units |
$385.00 |
|
42120-1 |
VeriKine Mouse Interferon Alpha ELISA Kit Compatibility: Tissue Culture Media Assay Range: 12.5-400 pg/ml |
1 x 96-well plate |
$505.00 |
|
42120-2 |
VeriKine Mouse Interferon Alpha ELISA Kit Compatibility: Tissue Culture Media Assay Range: 12.5-400 pg/ml |
5 x 96-well plate |
$2,345.00 |
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PBL Assay Science offers scientists a comprehensive range of assay kits, interferons, cytokines, growth factors and antibodies. Our high quality products provide consistent, reliable performance and are suitable for use in a range of research applications.
Quick Search
PBL’s improved site search function is defaulted to an exact match.
Search Term Results |
|
IFN beta |
All interferon beta products and related technical information (e.g. technical notes) |
Human IFN beta |
All human interferon products related technical information (e.g. technical notes) |
IL-2 |
All products contain the term “IL-2”, including IL-23 |
IL 2 |
All products contain the term “IL 2”, including IL-23 |
Erythropoietin |
All EPO products and technical information |
Eryth |
All EPO products and technical information |
The results are displayed in best match products grouped in the following product category filters:
Each filter contains sub-categories for further narrowing of your search results
Other web content such as technical notes that contain the Search Term can be displayed by checking the box next to the corresponding filter on the left side of the Search Results Page.