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Mouse IFN-Alpha ELISA Kit (TCM)

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Mouse IFN-Alpha ELISA Kit (TCM)

Catalog Number: 42120

This well-cited TCM-compatible ELISA detects all 14 mouse IFN-Alpha subtypes to an LLOQ of 12.5 pg/ml. This immunoassay is based on the international reference standard for mouse IFN-Alpha.


Product Name: VeriKine Mouse Interferon-Alpha ELISA Kit (TCM)


Pack Size
Product Info

Matrix CompatibilityTissue Culture Media (TCM) only
Assay Range12.5 - 400 pg/ml
Assay Length27 hours, 15 minutes
SpecificityMouse Interferon Alpha



The Mouse IFN-Alpha ELISA kit (cat. no. 42120) manufactured by PBL is designed to quantitate levels of IFN-Alpha in cell/tissue culture media (10% FBS) using a sandwich immunoassay. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish per-oxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for mouse interferon alpha (Mu-IFN-Alpha) provided by the National Institutes of Health.


It is important with this kit and any ELISA kit to run the standard curve in the same matrix as your samples unless you have demonstrated that the matrix does not affect the signal.


Note: The quantitation of mouse IFN-Alpha in serum and plasma samples using this product has not been fully evaluated by PBL.


*For additional sensitivity and/or complex biological matrices such as plasma and serum, we recommend our Mouse IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 42115-1).


CVs and Spike Recovery

Inter-Assay < 10%

Intra-Assay < 10%


Average Spike Recovery: > 115%


No cross-reactivity against

  • Rat IFN-Alpha
  • Mouse IFN-Beta, IFN-Gamma
  • Human IFN-Alpha
SynonymsMouse IFN Alpha Subtype, Mouse IFN Protein Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha
Expiration Date15 months from the date of manufacture
Shipping ConditionWet Ice



Materials Provided

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Alpha (IFN-Alpha) Standard, 10,000 pg/ml
  • Sample Buffer
  • Concentrate Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Assay Diluent
  • TMB Substrate Solution
  • Stop Solution


Additional Materials Required (Not Provided) 

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data

Application Note:


Technical Note (by request)

  • VeriKine Mouse Interferon Alpha ELISA Kit Use with Tissue Homogenates




Interferons (IFNs) are a group of cytokines which exhibit pleitropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple IFN-α genes and at least one IFN-β gene in most vertebrates, and a few other family members such as limitin in the mouse.1 There are 14 IFN-α subtypes in the mouse which share at least 75% identity in the protein sequence. In the human, IFN-α is used to treat viral diseases and cancer2-4 and is studied in mouse models of these diseases.5,6 Recent evidence has suggested that IFN-α may play a role in the development of certain autoimmune diseases7,8 and that treatment with IFN-α is useful for other autoimmune diseases.9


The expression of interferons is regulated by a group of interferon regulatory factor proteins (IRF). The expression of the individual IFN-α subtypes is regulated by the cellular levels of IRF3 and IRF7. Most of the IFN-α subtypes require IRF7 for expression. IRF7 is constitutively expressed in some cell types and is inducible in others. Inducible IRF7 expression is induced by IFN-β and IFN-α4 in mice and leads to expression of the other IFN-α subtypes. Murine IFN-β may be required for the production of IFN-α by fibroblasts but may not be required for IFN-α production by other cells.10 The IRF3/IRF7 signaling cascade is important for the initial and progressive responses to pathogens wherein hundreds of genes are regulated in a coordinated, temporal manner.11


Plasmacytoid dendritic cells are the major producers of IFN-α, but a large variety of cells can also produce lower levels of these proteins.12,13 The particular subtypes that are expressed appear to be somewhat cell and stimulus specific.14-16




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Background Literature:


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42120-1 CoA & Protocol (One-Page)

42120-1 Certificate of Analysis (CoA) & Protocol (One-Page)

42120-2 CoA & Protocol (One-Page)

42120-2 Certificate of Analysis (CoA) & Protocol (One-Page)

42120 SDS

42120 Safety Data Sheet

42120 Product Flyer

42120 Product Flyer

42120 Protocol (Full)

42120 Protocol (Full)