Skip to main content

Mouse IFN-Alpha 4, mammalian

Hero Region
Welcome to the new PBL Assay Science website!

We'd love it if you could browse around and let us know what you think -- we're a small team and appreciate the extra eyes. 

While you're browsing, if you come across any products you'd like to purchase, we're offering a month-long promotion with code: NEWSITE15

Mouse IFN-Alpha 4, mammalian

Catalog Number: 12115

Recombinant Mouse Interferon Alpha 4 protein expressed in mammalian cells. 

$420.00

Pack Size
Product Info

Mouse IFN-Alpha 4 is a widely expressed mouse IFN-Alpha which may play a role in preparing cells to express high levels of other mouse interferon alpha subtypes upon viral infection.

 

  • Exhibits higher antiviral potency than Mouse IFN-Alpha A in standard CPE assay
  • Authentic sequence provides a better model of native Mouse IFN-Alpha 4
  • Member of Mouse Type I IFN with early endogenous expression

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.5 kDa
Source Gene obtained from Murine (C57/BI) DNA expressed in mammalian cells
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
The EC50 for IFN in this assay is ~ 5 U/ml.
Storage For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles
Synonyms Mu IFN-α4, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha
Accession Number NM_010504

Tech Info & Data

Background

 

Mouse IFN-Alpha 4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mouse IFN-Alpha 4, like IFN Beta, has a priming function on cells, enabling the expression of other Mouse IFN-Alpha subtypes [Review by Mesplede et al., (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al., (2002) J. Exp. Med. 195(4):507].

 

Summary: Mouse IFN-Alpha 4 is a widely expressed mouse IFN-Alpha which may play a role in preparing cells to express high levels of other mouse interferon alpha subtypes upon viral infection.

 

For more information on Mouse IFN-Alpha subtype nomenclature click here.

Citations

7 Citations

 

  1. Lee, J.V. et al., (2022), Combinatorial immunotherapeuties overcome MYC-driven immune evasion in triple negative breast cancer, Nat. Commun., 13(1):3671, PMID: 35760778, DOI: 10.1038/s41467-022-31238-y (link)

  2. Throm, Allison, et al. (2018). Dysregulated NK Cell PLC-gamma-2 Signaling and Activity in Juvenile Dermatomyositis. JCI Insight, 14 pgs. PMID: 30429375. (link)

  3. Wang, Kuan-Chung (2018). Dichotomy of TNF Family Ligands Expression on Classical Dendritic Cells and Monocyte-Derived Antigen Presenting Cells During Viral Infection. University of Toronto, 92 pgs. PMID: no PMID. (link)

  4. Kalkavan, Halime, et al. (2017). Spatiotemporally Restricted Arenavirus Replication Induces Immune Surveillance and Type I Interferon-Dependent Tumour Regression. Nature Communications, 14 pgs. PMID: 28248314. (link)

  5. Shaabani, Namir, et al. (2016). CD169+ Macrophages Regulate PD-L1 Expression via Type I Interferon and Thereby Prevent Severe Immunopathology After LCMV Infection. Cell Death Differentiation Association, 10 pgs. PMID: 27809306. (link)

  6. Hu, MM, et al. (2015). TRIM38 Negatively Regulates TLR3/4-Mediated Innate Immune and Inflammatory Responses by Two Sequential and Distinct Mechanisms. Journal of Immunology, 12 pgs. PMID: 26392463. (link)

  7. Levy, David (2002). Whence Interferon? Variety in the Production of Interferon in Response to Viral Infection. JEM, 4 pgs. PMID: 11854366. (link)

 

References

 

  1. Van Pesch et al. 2004.

  2. Zwarthoff et al. (1985) Nuc. Acids Res. 13(3) 791.

  3. Mesplede et al. (2003) Autoimmunity 36(8):447.

  4. Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

  5. Barchet et al. (2002) J. Exp. Med. 195(4):507.

Documentation

Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
12115-1 CoA

12115-1 Certificate of Analysis (CoA)

12115 SDS

12115 Safety Data Sheet