Mouse IFN-Alpha 4, mammalian
Catalog Number: 12115
Recombinant Mouse Interferon Alpha 4 protein expressed in mammalian cells.
Mouse IFN-Alpha 4 is a widely expressed mouse IFN-Alpha which may play a role in preparing cells to express high levels of other mouse interferon alpha subtypes upon viral infection.
- Exhibits higher antiviral potency than Mouse IFN-Alpha A in standard CPE assay
- Authentic sequence provides a better model of native Mouse IFN-Alpha 4
- Member of Mouse Type I IFN with early endogenous expression
|Formulation||Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)|
|Molecular Weight||19.5 kDa|
|Source||Gene obtained from Murine (C57/BI) DNA expressed in mammalian cells|
|Purity||> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
|Bioactivity||Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
The EC50 for IFN in this assay is ~ 5 U/ml.
|Storage||For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles|
|Synonyms||Mu IFN-α4, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha|
Tech Info & Data
Mouse IFN-Alpha 4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mouse IFN-Alpha 4, like IFN Beta, has a priming function on cells, enabling the expression of other Mouse IFN-Alpha subtypes [Review by Mesplede et al., (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al., (2002) J. Exp. Med. 195(4):507].
Summary: Mouse IFN-Alpha 4 is a widely expressed mouse IFN-Alpha which may play a role in preparing cells to express high levels of other mouse interferon alpha subtypes upon viral infection.
For more information on Mouse IFN-Alpha subtype nomenclature click here.
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- Van Pesch et al. 2004.
- Zwarthoff et al. (1985) Nuc. Acids Res. 13(3) 791.
- Mesplede et al. (2003) Autoimmunity 36(8):447.
- Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.
- Barchet et al. (2002) J. Exp. Med. 195(4):507.