Mouse IFN Alpha 4, mammalian

Product Features: 
  • Recombinant Mouse IFN Alpha 4 protein expressed in mammalian cells
  • Exhibits higher antiviral potency than Mouse IFN Alpha A in standard CPE assay
  • Authentic sequence provides a better model of native Mouse IFN Alpha 4
  • Member of Mouse Type I IFN with early endogenous expression

Ordering

Catalog No. Pack Size Price Quantity
12115-1

1 x 105 units

$375.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.5 kDa
Source Gene obtained from Murine (C57/BI) DNA expressed in mammalian cells
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
The EC50 for IFN in this assay is ~ 5 U/ml.
Storage For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles
Synonyms Mu IFN-α4, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha
Accession Number NM_010504

Tech Info / Data

Titration of Mouse Interferon Alpha 4 (Mammalian) in the L929 Cell Line
Background: 

Mouse IFN Alpha 4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mouse IFN Alpha 4, like IFN Beta, has a priming function on cells, enabling the expression of other Mouse IFN Alpha subtypes [Review by Mesplede et al., (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al., (2002) J. Exp. Med. 195(4):507].

Summary: Mouse IFN Alpha 4 is a widely expressed mouse interferon alpha which may play a role in preparing cells to express high levels of other mouse interferon alpha subtypes upon viral infection.

For more information on Mouse IFN Alpha subtype nomenclature click here.

Citations

Comparison of Mouse IFN Alpha 4 and Mouse IFN Alpha A Antiviral Activity

12115-1 Mouse IFN Alpha 4

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