Mouse IFN Alpha 4, mammalian

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Product Features: 
  • Recombinant Mouse IFN Alpha 4 protein expressed in mammalian cells
  • Exhibits higher antiviral potency than Mouse IFN Alpha A in standard CPE assay
  • Authentic sequence provides a better model of native Mouse IFN Alpha 4
  • Member of Mouse Type I IFN with early endogenous expression

Ordering

Catalog No. Pack Size Price Quantity
12115-1

1 x 105 units

 $375.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.5 kDa
Source Gene obtained from Murine (C57/BI) DNA expressed in mammalian cells
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
The EC50 for IFN in this assay is ~ 5 U/ml.
Storage For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles
Synonyms Mu IFN-α4, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha
Accession Number NM_010504

Tech Info / Data

Titration of Mouse Interferon Alpha 4 (Mammalian) in the L929 Cell Line
Background: 

Mouse IFN Alpha 4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mouse IFN Alpha 4, like IFN Beta, has a priming function on cells, enabling the expression of other Mouse IFN Alpha subtypes [Review by Mesplede et al., (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al., (2002) J. Exp. Med. 195(4):507].

Summary: Mouse IFN Alpha 4 is a widely expressed mouse interferon alpha which may play a role in preparing cells to express high levels of other mouse interferon alpha subtypes upon viral infection.

For more information on Mouse IFN Alpha subtype nomenclature click here.

Citations

6 Citations:

  1. Throm, Allison, et al. (2018). Dysregulated NK Cell PLC-gamma-2 Signaling and Activity in Juvenile Dermatomyositis. JCI Insight, 14 pgs. PMID: 30429375. (link)

  2. Wang, Kuan-Chung (2018). Dichotomy of TNF Family Ligands Expression on Classical Dendritic Cells and Monocyte-Derived Antigen Presenting Cells During Viral Infection. University of Toronto, 92 pgs. PMID: no PMID. (link)

  3. Kalkavan, Halime, et al. (2017). Spatiotemporally Restricted Arenavirus Replication Induces Immune Surveillance and Type I Interferon-Dependent Tumour Regression. Nature Communications, 14 pgs. PMID: 28248314. (link)

  4. Shaabani, Namir, et al. (2016). CD169+ Macrophages Regulate PD-L1 Expression via Type I Interferon and Thereby Prevent Severe Immunopathology After LCMV Infection. Cell Death Differentiation Association, 10 pgs. PMID: 27809306. (link)

  5. Hu, MM, et al. (2015). TRIM38 Negatively Regulates TLR3/4-Mediated Innate Immune and Inflammatory Responses by Two Sequential and Distinct Mechanisms. Journal of Immunology, 12 pgs. PMID: 26392463. (link)

  6. Levy, David (2002). Whence Interferon? Variety in the Production of Interferon in Response to Viral Infection. JEM, 4 pgs. PMID: 11854366. (link)

References:

  1. Van Pesch et al. 2004.

  2. Zwarthoff et al. (1985) Nuc. Acids Res. 13(3) 791.

  3. Mesplede et al. (2003) Autoimmunity 36(8):447.

  4. Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

  5. Barchet et al. (2002) J. Exp. Med. 195(4):507.

Documentation

Comparison of Mouse IFN Alpha 4 and Mouse IFN Alpha A Antiviral Activity

12115-1 Mouse IFN Alpha 4

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