Mouse IFN Alpha A

Product Features: 
  • Mouse IFN Alpha A recombinant protein with activity > 1 x 105 U/ml
  • Distinct IFN-alpha subtype used extensively for research
  • Greater than 95% purity

Ordering

Catalog No. Pack Size Price Quantity
12100-1

1 x 105 units

$395.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.3 kDa
Source cDNA obtained from mouse leukocyte interferon expressed in E.coli
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.

EC50 for IFN Alpha A in this assay is ~ 5 U/ml.
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms Mouse Interferon Alpha, IFNA3, Interferon Alpha 3, IFNA, IFN-alpha A
Accession Number NP_996753

Tech Info / Data

Titration of Mouse Interferon Alpha A in the L929 Cell Line
Background: 

For more information on Mouse IFN Alpha subtype nomenclature click here.


Most mammalian species have multiple IFN-alpha subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the mouse IFN-alpha subtypes (van Pesch, et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mouse IFN-alpha A was deemed to have average antiviral activity when compared with the potency of the other subtypes.

Mouse IFN-alpha A was initially cloned by Daugherty, et al. (1984) J. Interferon Res. 4(4) 635]. Being the first commercially available Mouse IFN-alpha protein, it has been used extensively in published research reports. Expression of this subtype has been observed in neurons infected with Theiler’s virus [Delhaye, et al. (2006) PNAS 103(20)7835]. Additionally, the protein has been shown to sensitize lymphocytes to undergo apoptosis [Cuerro, et al. (2004) J. Exp. Med. 200(4)535] and enhance the proliferation and differentiation of CD8 T-cells [Curtsinger, et al. (2005) J. Immunol. 174(8) 4465]. Furthermore, it was used to determine that B10.Q/J mice are susceptible to infection due to a Tyk2 mutation [Shaw, et al.(2003) PNAS 100(20)11594]. Hardy, et al. [Genomics (2004) 84:331] recently suggested a name of IFN Alpha 3 based on sequence comparison of the Mouse IFN-alpha subtype family of proteins.

Citations

Antiviral Activities of PBL's Mouse IFN Alpha A (12100) and Mouse IFN Alpha 4 (12115) on Mouse L929 Cells Infected with EMCV

 

Image depicting the Antiviral Activities of Mouse Interferon Alpha A and Interferon Alpha 4 as determined in an L929/EMCV CPE assay

The activity of Mouse IFN Alpha A and Mouse IFN Alpha 4 was compared in an L929/EMCV CPE assay. The EC50 for Mouse Alpha A in this experiment was 54 pg/ml.

Results are representative and may vary depending upon experimental conditions.

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