Mouse IFN-Alpha A
Catalog Number: 12100
Mouse Interferon Alpha A recombinant protein with activity > 1 x 105 U/ml. This IFN-Alpha subtype is extensively used for research.
|Formulation||Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)|
|Molecular Weight||19.3 kDa|
|Source||cDNA obtained from mouse leukocyte interferon expressed in E.coli|
|Purity||> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
|Bioactivity||Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
EC50 for IFN Alpha A in this assay is ~ 5 U/ml.
|Storage||For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles|
|Synonyms||Mouse Interferon Alpha, IFNA3, Interferon Alpha 3, IFNA, IFN-alpha A|
Tech Info & Data
Most mammalian species have multiple IFN-Alpha subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the mouse IFN-Alpha subtypes (van Pesch, et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mouse IFN-Alpha A was deemed to have average antiviral activity when compared with the potency of the other subtypes.
Mouse IFN-Alpha A was initially cloned by Daugherty, et al. (1984) J. Interferon Res. 4(4) 635]. Being the first commercially available Mouse IFN-Alpha protein, it has been used extensively in published research reports. Expression of this subtype has been observed in neurons infected with Theiler’s virus [Delhaye, et al. (2006) PNAS 103(20)7835]. Additionally, the protein has been shown to sensitize lymphocytes to undergo apoptosis [Cuerro, et al. (2004) J. Exp. Med. 200(4)535] and enhance the proliferation and differentiation of CD8 T-cells [Curtsinger, et al. (2005) J. Immunol. 174(8) 4465]. Furthermore, it was used to determine that B10.Q/J mice are susceptible to infection due to a Tyk2 mutation [Shaw, et al.(2003) PNAS 100(20)11594]. Hardy, et al. [Genomics (2004) 84:331] recently suggested a name of IFN Alpha 3 based on sequence comparison of the Mouse IFN-Alpha subtype family of proteins.
For more information on Mouse IFN-Alpha subtype nomenclature click here.
- Klass, M. et al., (2023), "Sialoadhesin )CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions synergizes with other macrophage receptors to promote phagocytosis", Glycoconjugate J., PMID: 36738392, DOI: 10.1007/s10719-022-10097-1 (link)
- Zhu, J. et al., (2022), "LincRNA-EPS impairs host antiviral immunity by antagonizing viral RNA-PKR interaction", EMBO Reports, e53937, PMID: 35312340, DOI: 0.15252/embr.202153937 (link)
- Zhang, T., et al., (2022), "Mesenchymal stromal cells equipped by IFNa empower T cells with potent anti-tumor immunity", Oncogene, PMID: 35145233, DOI: 10.1038/s41388-022-02201-4 (link)
- Ka, N., et al., (2021), "IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer", Cell Reports, 37:110138, DOI: 10.1016/j.celrep.2021.110138 (link)
- Tang, Z. et al., (2021), "Inflammatory macrophages exploit unconventional pro-phagocytic integrins for phagocytosis and anti-tumor immunity", Cell Reports, 37:110111, DOI: 10.1016/j.celrep.2021.110111. (link)
- Zhang et al., (2020), "Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death", JEM 218(2), PMID: 33125053 (link)
- Li, et al. (2019). "Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation". JCI. PMID: 30830879. (link)
- Hernaez, et al. (2018). A virus-encoded type I interferon decoy receptor enables evasion of host immunity through cell-surface binding. Nature Communications, 14 pgs. PMID: 30575728. (link)
- Chaly, Yury, et al. (2018). Type I Interferon Signaling is Required for Dacryoadenitis in the Nonobese Diabetic Mouse Model of Sjorgen Syndrome. IJMS, 17 pgs. PMID: 30347820. (link)
- Chitre, Avantika, et al. (2018). A20 Upregulation During Treated HIV Disease is Associated with Intestinal Epithelial Cell Recovery and Function. PLOS Pathogens, 24 pgs. PMID: 29505600. (link)
- Wang, Jingya, et al. (2017). Distinct and Common Expression of Receptors for Inflammatory Mediators in Vagal Nodose Versus Jugular Capsaicin-Sensitive/TRPV1-Positive Neurons Detected by Low Input RNA Sequencing. PLOS One , 20 pgs. PMID: 28982197. (link)
- Stier, Matthew T, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology, 25 pgs. PMID: 28576981. (link)
- Moodley, Devapregasan, et al. (2016). Network pharmacology of JAK inhibitors. PNAS, 6 pgs. PMID: 27516546. (link)
- Ananth, Abhirami A, et al. (2016). Surgical Stress Abrogates Pre-Existing Protective T Cell Mediated Anti-Tumor Immunity Leading to Postoperative Cancer Recurrence. PLOS One, 19 pgs. PMID: 27196057. (link)
- Shou, et al. (2016). Type I interferons exert anti-tumor effect via reversing immunosuppression mediated by mesenchymal stromal cells. Nature, 10 pgs. PMID: 27109100. (link)
- Labzin, Larisa I, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)
- Kuriakose, Teneema, et al. (2015). Tumor Progression Locus 2 Promotes Induction of IFNλ, Interferon Stimulated Genes and Antigen-Specific CD8+ T Cell Responses and Protects against Influenza Virus.. PLOS One, 22 pgs. PMID: 26241898. (link)
- Onabajo, Olusegun, et al. (2015). Expression of Interferon Lambda 4 is Associated with Reduced Proliferation and Increased Cell Death in Human Hepatic Cells. Journal of Interferon & Cytokine Research, 13 pgs. PMID: 26134097. (link)
- Sheehan, Kathleen, et al. (2015). Selective Blockade of Interferon-alpha and -beta Reveals Their Non-Redundant Functions in a Mouse Model of West Nile Virus Infection. PLOS One, 19 pgs. PMID: 26010249. (link)
- Hong, Bangxing, et al. (2014). USP18 is crucial for IFN-γ-mediated inhibition of B16 melanoma tumorigenesis and antitumor immunity. Molecular Cancer Research, 12 pgs. PMID: 24884733. (link)
- Yao, Huilan, et al. (2014). Corepressor Rcor1 is essential for murine erythropoiesis. Blood Journal. PMID: 24652990. (link)
- Bezbradica, Jelena S, et al. (2014). A role for the ITAM signaling module in specifying cytokine-receptor functions. Nature Immunology, 29 pgs. PMID: 24608040. (link)
- Bosmann, Markus, et al. (2014). Tyrosine kinase 2 promotes sepsis-associated lethality by facilitating production of interleukin-27. JLB. PMID: 24604832. (link)
- Jefferson, Matthew, et al. (2014). The Pestivirus N Terminal Protease N^(pro) Redistributes to Mitochondria and Peroxisomes Suggesting New Sites for Regulation of IRF3 by N^(pro). PLOS One, 8 pgs. PMID: 24551175. (link)
- Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)
- Trilling, et al. (2014). "Activated" STAT Proteins: A Paradoxical Consequence of Inhibited JAK-STAT Signaling in Cytomegalovirus-Infected Cells. Journal of Immunology, 13 pgs. PMID: 24319264. (link)
- Taylor, Juliet, et al. (2014). Type I Interferon Signaling Mediates Neuro-Inflammatory Events in Model of Alzheimer's Disease. Neurobiology of Aging, 12 pgs. PMID: 24262201. (link)
- Chalise, et al. (2013). Interferon Alpha Inhibits Antigen-Specific Production of Proinflammatory Cytokines and Enhances Antigen-Specific Transforming Growth Factor Beta Production in Antigen-Induced Arthritis. Arthritis Research & Therapy, 13 pgs. PMID: 24286140. (link)
- Pereira-Lopes, Selma, et al. (2013). The exonuclease Trex1 restrains macrophage proinflammatory activation. Journal of Immunology, 9 pgs. PMID: 24218451. (link)
- Kerenyi, Marc A, et al. (2013). Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. eLIFE, 23 pgs. PMID: 23795291. (link)
- Valentine, et al. (2012). CD8+ T Cell-Derived IFN-gamma Prevents Infection by a Second Heterologous Virus. Journal of Immunology, 19 pgs. PMID: 23136204. (link)
- Freeman, Bailey E, et al. (2012). Regulation of innate CD8+ T-cell activation mediated by cytokines. PNAS, 6 pgs. PMID: 22665806. (link)
- Krause, et al. (2011). Efficient Co-Expression of Bicistronic Proteins in Mesenchymal Stem Cells by Development and Optimization of a Multifunctional Plasmid. Stem Cell Research & Therapy, 20 pgs. PMID: 21401924. (link)
- Lenert, et al. (2009). DNA-like Class R Inhibitory Oligonucleotides (INH-ODNs) Preferentially Block Autoantigen-Induced B-Cell and Dendritic Cell Activation In Vitro and Autoantibody Production in Lupus-Prone MRL-Faslpr/lpr Mice In Vivo. Arthritis Research & Therapy, 16 pgs. PMID: 19476613. (link)
- Trilling, Mirko, et al. (2009). Gamma Interferon-Induced Interferon Regulatory Factor 1-Dependent Antiviral Response Inhibits Vaccinia Virus Replication in Mouse but Not Human Fibroblasts. JVI, 36 pgs. PMID: 19211768. (link)
- Yamada, Atsushi, et al. (2009). IFN-gamma down-regulates Secretoglobin 3A1 gene expression. Biochemical and Biophysical Research Communications, 9 pgs. PMID: 19135978. (link)
- Guo, Beichu, et al. (2008). The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. JCI, 11 pgs. PMID: 18382764. (link)
- Vidy, et al. (2005). Rabies Virus P Protein Interacts with STAT1 and Inhibits Interferon Signal Transduction Pathways. JVI, 10 pgs. PMID: 16254375. (link)
- Best, Sonja M, et al. (2005). Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist. JVI, 12 pgs. PMID: 16188985. (link)
- Baban, et al. (2005). A Minor Population of Splenic Dendritic Cells Expressing CD19 Mediates IDO-Dependent T Cell Suppression via Type I IFN Signaling Following B7 Ligation. International Immunology. PMID: 15967784. (link)
- Curtsinger, Julie, et al. (2005). Type I IFNs provide a third signal to CD8 T cells to stimulate clonal expansion and differentiation. Journal of Immunology, 6 pgs. PMID: 15814665. (link)
- Prell, Rodney, et al. (2005). Administration of IFN-alpha enhances the efficacy of a granulocyte macrophage colony stimulating factor-secreting tumor cell vaccine. Cancer Research, 9 pgs. PMID: 15781661. (link)
- Pantelic, Ljiljana, et al. (2005). Differential Induction of Antiviral Effects against West Nile Virus in Primary Mouse Macrophages Derived from Flavivirus-Susceptible and Congenic Resistant Mice by Alpha/Beta Interferon and Poly(I-C). JVI, 12 pgs. PMID: 15650200. (link)
- Dikopoulos, et al. (2005). Type I IFN Negatively Regulates CD8+ T Cell Responses Through IL-10 Producing CD4+ T Regulatory 1 Cells. Journal of Immunology, 12 pgs. PMID: 15611232. (link)
- van Pesch, Vincent, et al. (2004). Characterization of the Murine Alpha Interferon Gene Family. JVI, 10 pgs. PMID: 15254193. (link)
- Sakurai, Takuma, et al. (2002). Effect of coadministration of M-CSF and IFN-alpha on NK1.1+ cells in mice. Journal of Interferon & Cytokine Research, 8 pgs. PMID: 12162882. (link)
- Daugherty et al. (1984) J. Interferon Res. 4(4) 635.
- Delhaye et al.( 2006) PNAS 103(20)7835.
- Cuerro et al. (2004) J. Exp. Med. 200(4)535.
- Curtsinger et al. (2005) J. Immunol. 174(8) 4465.