Background

Most mammalian species have multiple IFN-Alpha subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the mouse IFN-Alpha subtypes (van Pesch, et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mouse IFN-Alpha A was deemed to have average antiviral activity when compared with the potency of the other subtypes.

Mouse IFN-Alpha A was initially cloned by Daugherty, et al. (1984) J. Interferon Res. 4(4) 635]. Being the first commercially available Mouse IFN-Alpha protein, it has been used extensively in published research reports. Expression of this subtype has been observed in neurons infected with Theiler’s virus [Delhaye, et al. (2006) PNAS 103(20)7835]. Additionally, the protein has been shown to sensitize lymphocytes to undergo apoptosis [Cuerro, et al. (2004) J. Exp. Med. 200(4)535] and enhance the proliferation and differentiation of CD8 T-cells [Curtsinger, et al. (2005) J. Immunol. 174(8) 4465]. Furthermore, it was used to determine that B10.Q/J mice are susceptible to infection due to a Tyk2 mutation [Shaw, et al.(2003) PNAS 100(20)11594]. Hardy, et al. [Genomics (2004) 84:331] recently suggested a name of IFN Alpha 3 based on sequence comparison of the Mouse IFN-Alpha subtype family of proteins.

For more information on Mouse IFN-Alpha subtype nomenclature click here.

47 Citations

1. Klass, M. et al., (2023), "Sialoadhesin )CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions synergizes with other macrophage receptors to promote phagocytosis", Glycoconjugate J., PMID: 36738392, DOI: 10.1007/s10719-022-10097-1 (link)


2. Zhu, J. et al., (2022), "LincRNA-EPS impairs host antiviral immunity by antagonizing viral RNA-PKR interaction", EMBO Reports, e53937, PMID: 35312340, DOI: 0.15252/embr.202153937 (link)


3. Zhang, T., et al., (2022), "Mesenchymal stromal cells equipped by IFNa empower T cells with potent anti-tumor immunity", Oncogene, PMID: 35145233, DOI: 10.1038/s41388-022-02201-4 (link)


4. Ka, N., et al.,  (2021), "IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer", Cell Reports, 37:110138, DOI: 10.1016/j.celrep.2021.110138 (link)


5. Tang, Z. et al., (2021), "Inflammatory macrophages exploit unconventional pro-phagocytic integrins for phagocytosis and anti-tumor immunity", Cell Reports, 37:110111, DOI: 10.1016/j.celrep.2021.110111. (link)


6. Zhang et al., (2020), "Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death", JEM 218(2), PMID: 33125053 (link)


7. Li, et al. (2019). "Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation". JCI. PMID: 30830879. (link)


8. Hernaez, et al. (2018). A virus-encoded type I interferon decoy receptor enables evasion of host immunity through cell-surface binding. Nature Communications, 14 pgs. PMID: 30575728. (link)


9. Chaly, Yury, et al. (2018). Type I Interferon Signaling is Required for Dacryoadenitis in the Nonobese Diabetic Mouse Model of Sjorgen Syndrome. IJMS, 17 pgs. PMID: 30347820. (link)


10. Chitre, Avantika, et al. (2018). A20 Upregulation During Treated HIV Disease is Associated with Intestinal Epithelial Cell Recovery and Function. PLOS Pathogens, 24 pgs. PMID: 29505600. (link)


11. Wang, Jingya, et al. (2017). Distinct and Common Expression of Receptors for Inflammatory Mediators in Vagal Nodose Versus Jugular Capsaicin-Sensitive/TRPV1-Positive Neurons Detected by Low Input RNA Sequencing. PLOS One , 20 pgs. PMID: 28982197. (link)


12. Stier, Matthew T, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology, 25 pgs. PMID: 28576981. (link)


13. Moodley, Devapregasan, et al. (2016). Network pharmacology of JAK inhibitors. PNAS, 6 pgs. PMID: 27516546. (link)


14. Ananth, Abhirami A, et al. (2016). Surgical Stress Abrogates Pre-Existing Protective T Cell Mediated Anti-Tumor Immunity Leading to Postoperative Cancer Recurrence. PLOS One, 19 pgs. PMID: 27196057. (link)


15. Shou, et al. (2016). Type I interferons exert anti-tumor effect via reversing immunosuppression mediated by mesenchymal stromal cells. Nature, 10 pgs. PMID: 27109100. (link)


16. Labzin, Larisa I, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)


17. Kuriakose, Teneema, et al. (2015). Tumor Progression Locus 2 Promotes Induction of IFNλ, Interferon Stimulated Genes and Antigen-Specific CD8+ T Cell Responses and Protects against Influenza Virus.. PLOS One, 22 pgs. PMID: 26241898. (link)


18. Onabajo, Olusegun, et al. (2015). Expression of Interferon Lambda 4 is Associated with Reduced Proliferation and Increased Cell Death in Human Hepatic Cells. Journal of Interferon & Cytokine Research, 13 pgs. PMID: 26134097. (link)


19. Sheehan, Kathleen, et al. (2015). Selective Blockade of Interferon-alpha and -beta Reveals Their Non-Redundant Functions in a Mouse Model of West Nile Virus Infection. PLOS One, 19 pgs. PMID: 26010249. (link)


20. Hong, Bangxing, et al. (2014). USP18 is crucial for IFN-γ-mediated inhibition of B16 melanoma tumorigenesis and antitumor immunity. Molecular Cancer Research, 12 pgs. PMID: 24884733. (link)


21. Yao, Huilan, et al. (2014). Corepressor Rcor1 is essential for murine erythropoiesis. Blood Journal. PMID: 24652990. (link)


22. Bezbradica, Jelena S, et al. (2014). A role for the ITAM signaling module in specifying cytokine-receptor functions. Nature Immunology, 29 pgs. PMID: 24608040. (link)


23. Bosmann, Markus, et al. (2014). Tyrosine kinase 2 promotes sepsis-associated lethality by facilitating production of interleukin-27. JLB. PMID: 24604832. (link)


24. Jefferson, Matthew, et al. (2014). The Pestivirus N Terminal Protease N^(pro) Redistributes to Mitochondria and Peroxisomes Suggesting New Sites for Regulation of IRF3 by N^(pro). PLOS One, 8 pgs. PMID: 24551175. (link)


25. Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)


26. Trilling, et al. (2014). "Activated" STAT Proteins: A Paradoxical Consequence of Inhibited JAK-STAT Signaling in Cytomegalovirus-Infected Cells. Journal of Immunology, 13 pgs. PMID: 24319264. (link)


27. Taylor, Juliet, et al. (2014). Type I Interferon Signaling Mediates Neuro-Inflammatory Events in Model of Alzheimer's Disease. Neurobiology of Aging, 12 pgs. PMID: 24262201. (link)


28. Chalise, et al. (2013). Interferon Alpha Inhibits Antigen-Specific Production of Proinflammatory Cytokines and Enhances Antigen-Specific Transforming Growth Factor Beta Production in Antigen-Induced Arthritis. Arthritis Research & Therapy, 13 pgs. PMID: 24286140. (link)


29. Pereira-Lopes, Selma, et al. (2013). The exonuclease Trex1 restrains macrophage proinflammatory activation. Journal of Immunology, 9 pgs. PMID: 24218451. (link)


30. Kerenyi, Marc A, et al. (2013). Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. eLIFE, 23 pgs. PMID: 23795291. (link)


31. Valentine, et al. (2012). CD8+ T Cell-Derived IFN-gamma Prevents Infection by a Second Heterologous Virus. Journal of Immunology, 19 pgs. PMID: 23136204. (link)


32. Freeman, Bailey E, et al. (2012). Regulation of innate CD8+ T-cell activation mediated by cytokines. PNAS, 6 pgs. PMID: 22665806. (link)


33. Krause, et al. (2011). Efficient Co-Expression of Bicistronic Proteins in Mesenchymal Stem Cells by Development and Optimization of a Multifunctional Plasmid. Stem Cell Research & Therapy, 20 pgs. PMID: 21401924. (link)


34. Lenert, et al. (2009). DNA-like Class R Inhibitory Oligonucleotides (INH-ODNs) Preferentially Block Autoantigen-Induced B-Cell and Dendritic Cell Activation In Vitro and Autoantibody Production in Lupus-Prone MRL-Faslpr/lpr Mice In Vivo. Arthritis Research & Therapy, 16 pgs. PMID: 19476613. (link)


35. Trilling, Mirko, et al. (2009). Gamma Interferon-Induced Interferon Regulatory Factor 1-Dependent Antiviral Response Inhibits Vaccinia Virus Replication in Mouse but Not Human Fibroblasts. JVI, 36 pgs. PMID: 19211768. (link)


36. Yamada, Atsushi, et al. (2009). IFN-gamma down-regulates Secretoglobin 3A1 gene expression. Biochemical and Biophysical Research Communications, 9 pgs. PMID: 19135978. (link)


37. Guo, Beichu, et al. (2008). The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. JCI, 11 pgs. PMID: 18382764. (link)


38. Vidy, et al. (2005). Rabies Virus P Protein Interacts with STAT1 and Inhibits Interferon Signal Transduction Pathways. JVI, 10 pgs. PMID: 16254375. (link)


39. Best, Sonja M, et al. (2005). Inhibition of interferon-stimulated JAK-STAT signaling by a tick-borne flavivirus and identification of NS5 as an interferon antagonist. JVI, 12 pgs. PMID: 16188985. (link)


40. Baban, et al. (2005). A Minor Population of Splenic Dendritic Cells Expressing CD19 Mediates IDO-Dependent T Cell Suppression via Type I IFN Signaling Following B7 Ligation. International Immunology. PMID: 15967784. (link)


41. Curtsinger, Julie, et al. (2005). Type I IFNs provide a third signal to CD8 T cells to stimulate clonal expansion and differentiation. Journal of Immunology, 6 pgs. PMID: 15814665. (link)


42. Prell, Rodney, et al. (2005). Administration of IFN-alpha enhances the efficacy of a granulocyte macrophage colony stimulating factor-secreting tumor cell vaccine. Cancer Research, 9 pgs. PMID: 15781661. (link)


43. Pantelic, Ljiljana, et al. (2005). Differential Induction of Antiviral Effects against West Nile Virus in Primary Mouse Macrophages Derived from Flavivirus-Susceptible and Congenic Resistant Mice by Alpha/Beta Interferon and Poly(I-C). JVI, 12 pgs. PMID: 15650200. (link)


44. Dikopoulos, et al. (2005). Type I IFN Negatively Regulates CD8+ T Cell Responses Through IL-10 Producing CD4+ T Regulatory 1 Cells. Journal of Immunology, 12 pgs. PMID: 15611232. (link)


45. van Pesch, Vincent, et al. (2004). Characterization of the Murine Alpha Interferon Gene Family. JVI, 10 pgs. PMID: 15254193. (link)


46. Sakurai, Takuma, et al. (2002). Effect of coadministration of M-CSF and IFN-alpha on NK1.1+ cells in mice. Journal of Interferon & Cytokine Research, 8 pgs. PMID: 12162882. (link)

References: 

1. Daugherty et al. (1984) J. Interferon Res. 4(4) 635.


2. Delhaye et al.( 2006) PNAS 103(20)7835.


3. Cuerro et al. (2004) J. Exp. Med. 200(4)535.


4. Curtsinger et al. (2005) J. Immunol. 174(8) 4465.