Mouse IFN-Alpha 1
Catalog Number: 12105
Mouse Interferon Alpha 1 protein with antiviral, antiproliferative, and immunomodulatory activities.
Mouse IFN-Alpha 1 is an interferon with broad tissue expression which may be highly effective against certain viruses such as CMV and HSV.
|Formulation||Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)|
|Molecular Weight||19.1 kDa|
|Source||Gene obtained from mouse DNA expressed in E.coli|
|Purity||> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
|Bioactivity||Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
The EC50 for IFN in this assay is ~ 5 U/ml.
|Storage||For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles|
|Synonyms||Mu IFN-α1, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha|
Tech Info & Data
Mouse IFN-Alpha 1 was originally cloned from a genomic library by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791]. It is produced by macrophages, neutrophils, plasmacytoid dendritic cells (pDCs) and other somatic cells in response to many viral and some pathogen infections.
Expression of the Mouse IFN-Alpha 1 subtype has been seen in embryonic fibroblasts in some studies, but not in others. Expression has also been observed in brain and in L929 cells challenged with a variety of viruses [Yeow et al. (1997) Int. J. Biochem. Cell Biol. 29(3):519, Barchet et al., (2002) J. Exp. Med. 195(4):507, Delhaye et al., (2006) PNAS. 103(20):7835, Fung et al., (2004) J. Imm. Meth. 284:177]. It has been suggested that expression of this IFN may be regulated by Oct-1 and that it is IRF-7 dependent [Mesplede et al., (2005) Mol. Cell. 25(19):8717, Sato et al., (2000) Immunity 13:539].
This subtype has been extensively studied either in transgenic mice or by delivery as an expression construct [Hekman et al., (1988) J. Biol. Chem. 263(24):12151, Carr et al., (1988) J. Immunol. 169(9):5859]. When delivered as an expression construct, Mouse IFN-Alpha 1 reduced CMV intramuscular infection significantly better than Mouse IFN-Alpha 4 or 9 [Yeow et al., (1988) J. Immunol. 160(6):2932].
Summary: Mouse IFN-Alpha 1 is an interferon with broad tissue expression which may be highly effective against certain viruses such as CMV and HSV.
For more information on Mouse IFN-Alpha subtype nomenclature click here.
- Jhala, G. et al., (2022), Interferons limit autoantigen-specific CD8+ T-cell expansion in the non-obese diabetic mouse, Cell Reports, 39(4):110747, PMID: 35476976, DOI: 10.1016/j.celrep.2022.110747 (link)
- Geary, CD, et al. (2018). Non-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection. Cell Reports. PMID: 30134157. (link)
- Dickensheets, Harold, et al. (2012). Interferon-lambda Induces Signal Transduction and Gene Expression in Human Hepatocytes but Not in Lymphocytes or Monocytes. JLB, 9 pgs. PMID: 23258595. (link)
- Park and Scott (2010). Cholesterol 25-Hydroxylase Production by Dendritic Cells and Macrophages is Regulated by Type I Interferons. JLB. PMID: 20699362. (link)
- Zwarthoff et al. (1985) Nuc. Acids Res. 13(3):791.
- Yeow et al. (1997) Int. J. Biochem. Cell Biol. 29(3):519.
- Barchet et al. (2002) J. Exp. Med. 195(4):507.
- Delhaye et al. (2006) PNAS.103 (20):7835.
- Hekman et al. (1988) J. Biol. Chem 263(24):12151.