Mouse IFN Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

Product Features: 
  • Quantifies low level mouse IFN-beta in mouse serum, mouse plasma, and tissue culture media (TCM) with an assay range of 0.94-60 pg/ml
  • Reliable serum and plasma spike recoveries of > 90%
  • Provides reproducible results with inter- & intra-assay CVs of < 10%
  • Total assay time of ~2 hours

Full Product Name: VeriKine-HS Mouse Interferon Beta Serum ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
42410-1

1 x 96-well plate

$525.00
42410-2

5 x 96-well plate

$1,995.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.94 - 60 pg/ml
Speed: Incubation time, 1 hour, 50 minutes
Specificity: Mouse Interferon Beta (No cross-reactivity against mouse: IFN-α A, IFN-α 4. IFN-α 11. IFN-α 13, IFN-γ, IL-6, Limitin, TNF-α; human: IFN-α A, IFN-β, IFN-γ, IFN-ω; rat: IFN-α, IFN-β, IFN-γ; swine: IFN-α, IFN-β)
Synonyms: Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta, Mouse IFN B

 

Inter-Assay CV: ≤ 7%
Intra-Assay CV: ≤ 6%
Spike Recovery: 95%

 

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice


Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Beta Standard, 10,000 pg/ml
  • Sample Diluent
  • Serum Buffer 
  • Antibody Concentrate 
  • Antibody Diluent
  • HRP Conjugate Concentrate 
  • HRP Diluent
  • TMB Substrate 
  • Stop Solution 

Tech Info / Data

Mouse IFN beta standard curve from 0.94 to 60 pg/ml using PBL High Sensitivity Mouse Interferon Beta ELISA (42410)
PBL High Sensitivity Mouse Interferon Beta ELISA (42410) detects Mammalian Cell- and E.coli-Expressed Mouse Interferon Beta
Intra- & Inter-Assay CVs and Average % Recoveries using PBL High Sensitivity Mouse Interferon Beta ELISA kit (42410)
Background: 

PBL's 96-well high sensitivity ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-β in serum and plasma matrices. This kit quantitates Mouse Interferon Beta (IFN-β) in sera, plasma and tissue culture media by sandwich enyzme linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. Purified, recombinant mouse IFN-β expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.


The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferons (IFNs) are a group of cytokines which exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates. Interferon Beta plays a pivotal role in the protective response to many infections and diseases due to its antiviral and immune-modulatory activities, and is an important early product of TLR/RLR stimulation. However, when produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes. Additionally, IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice.

Citations

Typical Standard Curves in a Variety of Matrices for PBL's High Sensitivity Mouse IFN Beta ELISA (42410) and a Competitor ELISA in Assay Buffer

 

Mouse IFN beta standard curves in different matrices using PBL HS Mouse IFN Beta ELISA (42410) vs a competitor ELISA in diluent

*Competitor data obtained from public source, provided for comparison only.

Related Products

Catalog No. Description Pack Size Price Quantity
42115-1 VeriKine-HS Mouse Interferon Alpha All Subtype ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 2.38 - 152 pg/ml

1 x 96-well plate

$595.00
$685.00
42400-1 VeriKine Mouse Interferon Beta ELISA Kit
Compatibility: Tissue Culture Media, Serum
Assay Range: 15.6-1000 pg/ml

1 x 96-well plate

$725.00
42400-2 VeriKine Mouse Interferon Beta ELISA Kit
Compatibility: Tissue Culture Media, Serum
Assay Range: 15.6-1000 pg/ml

5 x 96-well plate

$2,695.00
$2,995.00

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