Mouse IFN Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

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Product Features: 
  • Quantifies low level mouse IFN-beta in mouse serum, mouse plasma, and tissue culture media (TCM) with an assay range of 0.94-60 pg/ml
  • Reliable serum and plasma spike recoveries of > 90%
  • Provides reproducible results with inter- & intra-assay CVs of < 10%
  • Total assay time of ~2 hours

Full Product Name: VeriKine-HS Mouse Interferon Beta Serum ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
42410-1

1 x 96-well plate

 $525.00
42410-2

5 x 96-well plate

 $1,995.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.94 - 60 pg/ml
Speed: Incubation time, 1 hour, 50 minutes
Specificity: Mouse Interferon Beta (No cross-reactivity against mouse: IFN-α A, IFN-α 4. IFN-α 11. IFN-α 13, IFN-γ, IL-6, Limitin, TNF-α; human: IFN-α A, IFN-β, IFN-γ, IFN-ω; rat: IFN-α, IFN-β, IFN-γ; swine: IFN-α, IFN-β)
Synonyms: Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta, Mouse IFN B
 
Inter-Assay CV: ≤ 7%
Intra-Assay CV: ≤ 6%
Spike Recovery: 95%
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Beta Standard, 10,000 pg/ml
  • Sample Diluent
  • Serum Buffer 
  • Antibody Concentrate 
  • Antibody Diluent
  • HRP Conjugate Concentrate 
  • HRP Diluent
  • TMB Substrate 
  • Stop Solution 

Tech Info / Data

Image of mouse IFN beta standard concentration curve from 0.94 pg/ml to 60 pg/ml plotted against plotted against mean absorbance using PBL's High Sensitivity Mouse Interferon Beta ELISA kit
Image depicting the ability of PBL's High Sensitivity Mouse Interferon Beta ELISA kit to detect both Mammalian Cell- and E.coli-Expressed Mouse Interferon Beta
Image of table of Intra Assay and Inter Assay CV and Average % Recovery at Various Mouse IFN Beta Concentrations using PBL's High Sensitivity Mouse Interferon Beta ELISA kit

Detailed Related Product Information:

Background: 

PBL's 96-well high sensitivity ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-β in serum and plasma matrices. This kit quantitates Mouse Interferon Beta (IFN-β) in sera, plasma and tissue culture media by sandwich enyzme linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. Purified, recombinant mouse IFN-β expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.

The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferons (IFNs) are a group of cytokines which exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates. Interferon Beta plays a pivotal role in the protective response to many infections and diseases due to its antiviral and immune-modulatory activities, and is an important early product of TLR/RLR stimulation. However, when produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes. Additionally, IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice.

Citations

Selected Product Citations:

1.  Intrauterine Zika virus infection of pregnant immunocompetent mice models transplacental transmission and adverse perinatal outcomes. Vermillion et al., 2017, Nature Comm., doi:10.1038/ncomms14575.

2.  Invertebrate Iridescent Virus 6, a DNA Virus, Stimulates a Mammalian Innate Immune Response through RIG-I-Like Receptors, Ahlers et al., 2016, PLoS One, doi:10.1371/journal.pone.0166088.

3.  Viral DNA-Dependent Induction of Innate Immune Response to Hepatitis B Virus in Immortalized Mouse Hepatocytes. Cui et al., 2016, J. Virol., doi:10.1128/JVI.01263-15.

4.  Induction of the type I interferon response in neurological forms of Gaucher disease. Vitner et al., 2016, J. Neuroinflammation, doi:10.1186/s12974-016-0570-2.

5.  Cancer therapies activate RIG-I-Like receptor pathway through endogenous non-coding RNAs. Ranoa et al., 2016, Oncotarget, doi:10.18632/oncotarget.8420.

 

References:

  1. A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies. Staehelin et al., 1981, Methods in Enzymology (S. Peskta, ed.). 79: 589-595.

  2. A FADD-dependent innate immune mechanism in mammalian cells. Balachandran et al., 2004, Nature. 432: 401-405.

Documentation

Typical Standard Curves in a Variety of Matrices for PBL's High Sensitivity Mouse IFN Beta ELISA (42410) and a Competitor ELISA in Assay Buffer

 

Image of mouse IFN beta concentration plotted against mean absorbance in Sample Diluent, Mouse Serum, Mouse Plasma, and Tissue Culture Media using PBL's High Sensitivity Mouse Interferon Beta ELISA kit versus a Competitor ELISA in Assay Buffer

*Competitor data obtained from public source, provided for comparison only.

Related Products

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42400-1 VeriKine Mouse Interferon Beta ELISA Kit
Compatibility: Tissue Culture Media, Serum
Assay Range: 15.6-1000 pg/ml

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42400-2 VeriKine Mouse Interferon Beta ELISA Kit
Compatibility: Tissue Culture Media, Serum
Assay Range: 15.6-1000 pg/ml

5 x 96-well plate

 $2,695.00
$2,995.00

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