Mouse IFN Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

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Product Features: 

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  • Quantifies low level mouse IFN-beta in mouse serum, mouse plasma, and tissue culture media (TCM) with an assay range of 0.94-60 pg/ml
  • Reliable serum and plasma spike recoveries of > 90%
  • Provides reproducible results with inter- & intra-assay CVs of < 10%
  • Total assay time of ~2 hours

 

Full Product Name: VeriKine-HS Mouse Interferon Beta Serum ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
42410-1

1 x 96-well plate

 $525.00
42410-2

5 x 96-well plate

 $1,995.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.94 - 60 pg/ml
Speed: Incubation time, 1 hour, 50 minutes
Specificity: Mouse Interferon Beta (No cross-reactivity against mouse: IFN-α A, IFN-α 4. IFN-α 11. IFN-α 13, IFN-γ, IL-6, Limitin, TNF-α; human: IFN-α A, IFN-β, IFN-γ, IFN-ω; rat: IFN-α, IFN-β, IFN-γ; swine: IFN-α, IFN-β)
Synonyms: Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta, Mouse IFN B

 

Inter-Assay CV: ≤ 7%
Intra-Assay CV: ≤ 6%
Spike Recovery: 95%

 

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Beta Standard, 10,000 pg/ml
  • Sample Diluent
  • Serum Buffer 
  • Antibody Concentrate 
  • Antibody Diluent
  • HRP Conjugate Concentrate 
  • HRP Diluent
  • TMB Substrate 
  • Stop Solution 

Tech Info / Data

Mouse IFN beta standard curve from 0.94 to 60 pg/ml using PBL High Sensitivity Mouse Interferon Beta ELISA (42410)
PBL High Sensitivity Mouse Interferon Beta ELISA (42410) detects Mammalian Cell- and E.coli-Expressed Mouse Interferon Beta
Intra- & Inter-Assay CVs and Average % Recoveries using PBL High Sensitivity Mouse Interferon Beta ELISA kit (42410)
Background: 

PBL's 96-well high sensitivity ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-β in serum and plasma matrices. This kit quantitates Mouse Interferon Beta (IFN-β) in sera, plasma and tissue culture media by sandwich enyzme linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. Purified, recombinant mouse IFN-β expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.

The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferons (IFNs) are a group of cytokines which exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates. Interferon Beta plays a pivotal role in the protective response to many infections and diseases due to its antiviral and immune-modulatory activities, and is an important early product of TLR/RLR stimulation. However, when produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes. Additionally, IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice.

Citations

30 Citations:

  1. Mine, Keiichiro, et al. (2019). Impaired upregulation of Stat2 gene restrictive to pancreatic β-cells is responsible for virus-induced diabetes in DBA/2 mice. Biochemical and Biophysical Research Communications, 8 pgs. PMID: 31708097. (link)

  2. Schadt, Linda, et al. (2019). Cancer-Cell-Intrinsic cGAS Expression Mediates Tumor Immunogenicity. Cell Reports, 21 pgs. PMID: 31665636. (link)

  3. Miyauchi, et al. (2019). Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice. Toxicology Reports, 27 pgs. PMID: 31245279. (link)

  4. Kim, et al. (2019). Small Heterodimer Partner Controls the Virus-Mediated Antiviral Immune Response by Targeting CREB-Binding Protein in the Nucleus. Cell Reports, 20 pgs. PMID: 31091449. (link)

  5. Pang, Zheng (2019). Regulation of Inflammation During Pseudomonas Aeruginosa Lung Infection. Dalhousie University, 177 pgs. PMID: no PMID. (link)

  6. Kim, Sung Phil, et al. (2018). The composition of a bioprocessed shiitake (Lentinus edodes) mushroom mycelia and rice bran formulation and its antimicrobial effects against Salmonella enterica subsp. enterica serovar Typhimurium strain SL1344 in macrophage cells and in mice. BMC Complementary and Alternative Medicine, 12 pgs. PMID: 30518352. (link)

  7. Zhang, Lele, et al. (2018). The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway. PLOS Pathogens, 23 pgs. PMID: 30388174. (link)

  8. Inoue, Kazuki, et al. (2018). Bone protection by inhibition of microRNA-182. Nature Communications, 17 pgs. PMID: 30291236. (link)

  9. Chaudhary, Omkar, et al. (2018). Inhibition of p38MAPK in combination with ART reduces SIV-induced immune activation and provides additional protection from immune system deterioration. PLOS Pathogens, 29 pgs. PMID: 30161247. (link)

  10. Rossi, Matteo, et al. (2017). Type I interferons induced by endogenous or exogenous viral infections promote metastasis and relapse of leishmaniasis. PNAS, 6 pgs. PMID: 28439019. (link)

  11. Vermillion, et al. (2017). Intrauterine Zika Virus Infection of Pregnant Immunocompetent Mice Models Transplacental Transmission and Adverse Perinatal Outcomes. Nature Communications, 14 pgs. PMID: 28220786. (link)

  12. Yamada, Taisho, et al. (2017). FICZ Exposure and Viral Infection in Mice. Bio-Protocol. PMID: no PMID. (link)

  13. Ahlers, et al. (2016). Invertebrate Iridescent Virus 6, a DNA Virus, Stimulates a Mammalian Innate Immune Response Through RIG-I-Like Receptors. PLOS One, 21 pgs. PMID: 27824940. (link)

  14. Vitner, et al. (2016). Induction of the Type I Interferon Response in Neurological Forms of Gaucher Disease. Journal of Neuroinflammation, 15 pgs. PMID: 27175482. (link)

  15. Ranoa, et al. (2016). Cancer Therapies Activate RIG-I-Like Receptor Pathway Through Endogenous Non-Coding RNAs. Oncotarget, 20 pgs. PMID: 27034163. (link)

  16. Fukui, Ryutaro, et al. (2016). Type I IFN Contributes to the Phenotype of Unc93b1D34A/D34A Mice by Regulating TLR7 Expression in B Cells and Dendritic Cells. Journal of Immunology, 13 pgs. PMID: 26621862. (link)

  17. Earl, Patricia, et al. (2016). Genetic Studies of the Susceptibility of Classical and Wild-Derived Inbred Mouse Strains to Monkeypox Virus. Virology, 11 pgs. PMID: 25791934. (link)

  18. Chen, Hao Hang Rachel (2016). The effect of pandemic influenza H1N1 viral infection on house dust mite sensitized mice. University of British Columbia, 87 pgs. PMID: no PMID. (link)

  19. Hoang, Hang Thi Thu (2016). Analysis of host genetic factors influencing susceptibility to influenza A infections using knockout mice. University of Veterinary Medicine Hannover, 137 pgs. PMID: no PMID. (no link)

  20. Rivera-Toledo, Evelyn, et al. (2015). Respiratory Syncytial Virus Persistence in Murine Macrophages Impairs IFN-β Response but Not Synthesis. Viruses, 14 pgs. PMID: 26501312. (link)

  21. Cui, et al. (2015). Viral DNA-Dependent Induction of Innate Immune Response to Hepatitis B Virus in Immortalized Mouse Hepatocytes. JVI, 11 pgs. PMID: 26491170. (link)

  22. Hargadon, Kristian, et al. (2015). Melanoma-derived factors alter the maturation and activation of differentiated tissue-resident dendritic cells. Immunology and Cell Biology, 15 pgs. PMID: 26010746. (link)

  23. Hanson, Melissa, et al. (2015). Nanoparticulate STING agonists are potent lymph node–targeted vaccine adjuvants. JCI, 15 pgs. PMID: 25938786. (link)

  24. Detje, Claudia, et al. (2015). Upon Intranasal Vesicular Stomatitis Virus Infection, Astrocytes in the Olfactory Bulb Are Important Interferon Beta Producers That Protect from Lethal Encephalitis. JVI, 8 pgs. PMID: 25540366. (link)

  25. White, Michael, et al. (2014). Apoptotic Caspases Suppress mtDNA-Induced STING-Mediated Type I IFN Production. CellPress, 27 pgs. PMID: 25525874. (link)

  26. Deng, Liufu, et al. (2014). STING-Dependent Cytosolic DNA Sensing Promotes Radiation-Induced Type I Interferon-Dependent Antitumor Immunity in Immunogenic Tumors. Immunity, 22 pgs. PMID: 25517616. (link)

  27. Lalani, Almin, et al. (2014). Myeloid Cell TRAF3 Regulates Immune Responses and Inhibits Inflammation and Tumor Development in Mice. Journal of Immunology, 36 pgs. PMID: 25422508. (link)

  28. O'Reilly, L. A., et al. (2014). Loss of c-REL but not NF-κB2 prevents autoimmune disease driven by FasL mutation. Cell Death & Differentiation, 12 pgs. PMID: 25361085. (link)

  29. Wolferstaetter, Michael, et al. (2014). Recombinant Modified Vaccinia Virus Ankara Generating Excess Early Double-Stranded RNA Transiently Activates Protein Kinase R and Triggers Enhanced Innate Immune Responses. JVI, 16 pgs. PMID: 25297997. (link)

  30. Rosenberger, Catherine, et al. (2014). Characterization of innate responses to influenza virus infection in a novel lung type I epithelial cell model. Journal of General Virology, 13 pgs. PMID: 24243730. (link)

    References:

    1. A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies. Staehelin et al., 1981, Methods in Enzymology (S. Peskta, ed.). 79: 589-595.

    2. A FADD-dependent innate immune mechanism in mammalian cells. Balachandran et al., 2004, Nature. 432: 401-405.

    Documentation

    Typical Standard Curves in a Variety of Matrices for PBL's High Sensitivity Mouse IFN Beta ELISA (42410) and a Competitor ELISA in Assay Buffer

     

    Mouse IFN beta standard curves in different matrices using PBL HS Mouse IFN Beta ELISA (42410) vs a competitor ELISA in diluent

    *Competitor data obtained from public source, provided for comparison only.

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