Typical Standard Curves in a Variety of Matrices for PBL's High Sensitivity Mouse IFN-Beta ELISA (42410) and a Competitor ELISA in Assay Buffer
Typical Standard Curve from 0.94 to 600 pg/ml for PBL's High Sensitivity Mouse IFN-Beta ELISA (42410)
Comparison of Mammalian Cell- and E.coli-Expressed Mouse IFN-Beta Detection using PBL's High Sensitivity Mouse IFN-Beta ELISA (42410)
Intra- & Inter-Assay CVs & Average Percent Recoveries using PBL's High Sensitivity Mouse IFN-Beta ELISA (42410)
Mouse IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)
Catalog Number: 42410
This Mouse Interferon Beta ELISA has an LLOQ of 0.94 pg/ml and is compatible with serum, plasma, and tissue cultre media (TCM).
Product Name: VeriKine-HS Mouse Interferon-Beta ELISA Kit
$595.00
$645.00
Product Info
| Matrix Compatibility | Serum, Plasma, Tissue Culture Media (TCM) |
|---|---|
| Assay Range | 0.94 - 60 pg/ml |
| LLOQ |
0.94 pg/ml Need more sensitivity? Check out our Sample Testing Services |
| Assay Length | 1 hour, 50 minutes |
| Specificity | Mouse Interferon Beta |
PBL's high sensitivity ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-Beta in serum and plasma matrices. This kit quantitates Mouse Interferon Beta (IFN-Beta) in sera, plasma and tissue culture media by sandwich enzyme-linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate.
Purified, recombinant mouse IFN-Beta expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.
Specifications
| CVs and Spike Recovery |
Inter-Assay ≤ 7%
Average Spike Recovery: > 95% |
|---|---|
| Cross-reactivity |
No cross-reactivity against
|
| Synonyms | Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta, Mouse IFN B |
| Storage | 2-8°C |
| Expiration Date | One year from the date of manufacture |
| Shipping Conditions | Wet Ice |
Materials Provided
- Pre-coated microtiter plate(s)
- Plate Sealers
- Wash Solution Concentrate
- Mouse Interferon Beta Standard, 10,000 pg/ml
- Sample Diluent
- Serum Buffer
- Antibody Concentrate
- Antibody Diluent
- HRP Conjugate Concentrate
- HRP Diluent
- TMB Substrate
- Stop Solution
Additional Materials Required (Not Provided)
- Microplate reader capable of reading an OD at a wavelength of 450 nm
- Variable volume microtiter pipettes
- Adjustable multichannel pipette (50-300 μl)
- Reagent reservoirs
- Wash bottle or plate washing system
- Distilled or deionized water
- Serological pipettes (1, 5, 10 or 25 ml)
- Disposable pipette tips (polypropylene)
- Plate shaker
Tech Info & Data
Intra and Inter-Assay CVs to measure Precision |
|||
| Concentration | 2.5 | 10 | 50 |
|---|---|---|---|
| Intra-Assay CV (%) | 5.7 | 5.5 | 5.5 |
| Inter-Assay CV (%) | 8.3 | 6.3 | 4.8 |
| Average Recovery (%) | 98 | 92 | 94 |
Background
The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferons (IFNs) are a group of cytokines that exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates1,2.
Interferon Beta plays a pivotal role in the protective response to many infections and diseases3 due to its antiviral and immune-modulatory activities and is an important early product of TLR/RLR stimulation. However, when produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes4-6. Additionally, IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice7.
Citations
46 Citations:
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Zhang et al., (2020), Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death, JEM 218(2), PMID: 33125053 (link)
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Takenaka, Wataru, et al. (2020). Radiation Dose Escalation is Crucial in Anti-CTLA-4 Antibody Therapy to Enhance Local and Distant Antitumor Effect in Murine Osteosarcoma. Cancers, 15 pgs. PMID: 32545427. (link)
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Zuo, Pengfei, et al. (2020). Protease-activated receptor 2 deficiency in hematopoietic lineage protects against myocardial infarction through attenuated inflammatory response and fibrosis. Biochemical and Biophysical Research Communications, 8 pgs. PMID: 32204916. (link)
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Background Literature:
- A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies. Staehelin et al., 1981, Methods in Enzymology (S. Peskta, ed.). 79: 589-595.
- A FADD-dependent innate immune mechanism in mammalian cells. Balachandran et al., 2004, Nature. 432: 401-405.