Mouse IFN-Beta ELISA Kit, High Sensitivity (Serum, Plasma, TCM)
This Mouse Interferon Beta ELISA has an LLOQ of 0.94 pg/ml and is compatible with serum, plasma, and tissue cultre media (TCM).
Product Name: VeriKine-HS Mouse Interferon-Beta ELISA Kit
$595.00
$645.00
Product Info
Matrix Compatibility | Serum, Plasma, Tissue Culture Media (TCM) |
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Assay Range | 0.94 - 60 pg/ml |
LLOQ | 0.94 pg/ml Need more sensitivity? Check out our Sample Testing Services |
Assay Length | 1 hour, 50 minutes |
Specificity | Mouse Interferon Beta |
PBL's high sensitivity ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-Beta in serum and plasma matrices. This kit quantitates Mouse Interferon Beta (IFN-Beta) in sera, plasma and tissue culture media by sandwich enzyme-linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate.
Purified, recombinant mouse IFN-Beta expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.
Specifications
CVs and Spike Recovery | Inter-Assay ≤ 7%
Average Spike Recovery: > 95% |
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Cross-reactivity | No cross-reactivity against
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Synonyms | Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta, Mouse IFN B |
Storage | 2-8°C |
Expiration Date | One year from the date of manufacture |
Shipping Conditions | Wet Ice |
Materials Provided
- Pre-coated microtiter plate(s)
- Plate Sealers
- Wash Solution Concentrate
- Mouse Interferon Beta Standard, 10,000 pg/ml
- Sample Diluent
- Serum Buffer
- Antibody Concentrate
- Antibody Diluent
- HRP Conjugate Concentrate
- HRP Diluent
- TMB Substrate
- Stop Solution
Additional Materials Required (Not Provided)
- Microplate reader capable of reading an OD at a wavelength of 450 nm
- Variable volume microtiter pipettes
- Adjustable multichannel pipette (50-300 μl)
- Reagent reservoirs
- Wash bottle or plate washing system
- Distilled or deionized water
- Serological pipettes (1, 5, 10 or 25 ml)
- Disposable pipette tips (polypropylene)
- Plate shaker
Tech Info & Data
Intra and Inter-Assay CVs to measure Precision | |||
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Concentration | 2.5 | 10 | 50 |
Intra-Assay CV (%) | 5.7 | 5.5 | 5.5 |
Inter-Assay CV (%) | 8.3 | 6.3 | 4.8 |
Average Recovery (%) | 98 | 92 | 94 |
Background
The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferons (IFNs) are a group of cytokines that exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates1,2.
Interferon Beta plays a pivotal role in the protective response to many infections and diseases3 due to its antiviral and immune-modulatory activities and is an important early product of TLR/RLR stimulation. However, when produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes4-6. Additionally, IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice7.
Citations
57 Citations:
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- Cordeiro, B. et al., (2024), "Obesity intensifies sex-specific interferon signaling to selectively worsen central nervous system autoimmunity in females", Cell Metab., S1550-4131(24):00288-2, PMID: 39168127, DOI: 10.1016/j.cmet.2024.07017 (link)
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- Casella, V. et al., (2023), "Differential kinetics of splenic CD169+ macrophage death is one underlying cause of virus infection fate regulation", Cell Death Dis., 14(12):838, PMID: 38110339, DOI: 10.1038/s41419-023-06374-y (link)
- Tong et al., (2023). "Nucleotide modifications enable rational design of TLR7-selective ligands by blocking RNase cleavage", J. Exp Med., 221(2):e20230341, PMID: 38095631, DOI: 10.1084/jem.20230341 (link)
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- Uccello, T.P., et al., (2023), "New insights into the responder/nonresponder divide in rectal cancer: Damage-induced Type I IFNs dictate treatment efficacy and can be targeted to enhance radiotherapy", Cell death Dis., 14(7):470, PMID: 37495596, DOI: 10.1038/s41419-023-05999-3 (link)
- Liu, H. et al., (2023), "Activated cGAS/STING signaling elicits endothelial cell senescence in early diabetic retinopathy", JCI Insight, 8(12):e168945, PMID: 37345657, DOI: 10.1171/jci.insight.168945 (link)
- Li, N. et al., (2023), "STING controls opioidinduced itch and chronic itch via spinal tank-binding kinase 1-dependent type I interferon response in mice", J. Neuroinflammation, 20(1):101, PMID: 37122031, DOI: 10.1186/s12974-023-02783-0 (link)
- Udeochu, J.C. et al., (2023), "Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience", Nat. Neurosci., PMID: 37095396, DOI: 10.1038/s41593-023-01315-6 (link)
- Wahl, D., et al., (2023), "The reverse transcriptase inhibitor 3TC protects against age-related cognitive dysfunction", Aging Cell, e13798, PMID: 36949552, DOI: 10.1111/acel.13798 (link)
- Vornholz, L. et al., (2023), "Synthetic enforcement of STING signaling in cancer cells appropriates the immune microenvironment for checkpoint inhibitor therapy", Sci. Adv. 9(11):eadd8564, PMID: 36921054, DOI: 10.1126/sciadv.add8564 (link)
- Preston, S.P. et al., (2023), "A necroptosis-independent function of RIPK3 promotes immune dysfunction and prevents control of chronic LCMV infection", Cell Death Dis. 14(2):123, PMID: 36792599, DOI: 10.1038/s41419-023-05635-0 (link)
- Rodruguez-Ruiz, M.E. et al., (2023), "Intratumoral BO-112 in combination with radiotherapy synergizes to achieve CD8 T-cell-mediated local tumor control", J. Immunother. Cancer, 11(1):e005011, PMID: 36631161, DOI: 10.1136/jitc-2022-005011(link)
- Kwart, D. et al., (2022), "Cancer cell-derived type I interferons instruct tumor monocyte polarization", Cell Rep., 41(10):111769, PMID: 36476866, DOI:10.1016/j.celrep.2022.111769 (link)
- Chem, J. et al., (2022), "Age-induced prostaglandin E2 impairs mitochondrial fitness and increases mortality to influenza infection", Nat. Commun., 13(1):6759, PMID: 36351902, DOI: 10.1038/s41467-022-34593-y (link)
- Mansouri, S. et al., (2022), "MPYS Modulates Fatty Acid Metabolism and Immune Tolerance at Homeostasis Independent of Type I IFNs", J. Immunol., ji2200158, PMID: 36261171, DOI: 10.1049/jimmunol.2200158 (link)
- Xia, Y., et al., (2022), "TGFβ reprograms TNF stimulation of macrophages towards a non-cannonical pathway driving inflammatory osteoclastogenesis", Nat. Commun.,13(1):3920, PMID: 35798734, DOI: 10.1038/s41467-022-31475-1 (link)
- Mottis, A., et al., (2022), "Tetracycline-induced mitohormesis mediates disease tolerance against influenza", J. Clin. Invest. e:151540, PMID: 35787521, DOI: 10.1172/JCI151540 (link)
- Li, H. et al., (2022), "AXL targeting restores PD-1 blockade sensitivity of STK11/LKB1 mutant NSCLC through expansion of TCF1+ CD8 T cells". Cell Reports Medicine, 3:100554, DOI: 10.1016/j.xcrm.2022.100554 (link)
- Benoit-Lizon, I. et al., (2022), "CD4 cell-intrinsic STING signaling controls the differentiation and effector functions of TH1 and TH9 cells", Journal of Immuno Therapy Cancer, 10:e003459, DOI: 10.1136/jitc-2021-003459 (link)
- Yang, K. et al., (2021), "Suppression of local type I interferon by gut microbiota-derived butyrate impairs antitumor effects of ionizing radiation", JEM, 218(3):e20201915, PMID: 33496784, DOI: 10.1084/jem.20201915 (link)
- Baidya S et al., (2021), Dual Effect Organo - Germanium Compound THGP on RIG-I-Mediated Viral Sensing and Viral Replication during Influenza a Virus Infection, Viruses 13(9), (link)
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Background Literature:
- A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies. Staehelin et al., 1981, Methods in Enzymology (S. Peskta, ed.). 79: 589-595.
- A FADD-dependent innate immune mechanism in mammalian cells. Balachandran et al., 2004, Nature. 432: 401-405.