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Mouse IFN-Beta ELISA Kit (TCM, Serum)

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Mouse IFN-Beta ELISA Kit (TCM, Serum)

Catalog Number: 42400

This well-cited ELISA kit measures mouse Interferon Beta in tissue culture media (TCM) and mouse serum.

$615.00

Pack Size
Product Info

Matrix Compatibility Tissue Culture Media (TCM), Serum
Assay Range 15.6 - 1000 pg/ml
Assay Length 3 hours, 15 minutes
Specificity Mouse Interferon Beta

 

 

The VeriKine Mouse Interferon Beta ELISA Kit has been developed to quantitate levels of IFN-Beta in tissue culture media (TCM) or serum samples in a sandwich enzyme-linked immunosorbent assay (ELISA) format. Recombinant mouse IFN-Beta expressed in mammalian cells is provided as the standard. Interferons bind to plates coated with antibody and detection is accomplished using a secondary antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate.

 

*For increased sensitivity and/or complex biological matrices such as plasma or sera, we recommend our Mouse IFN-Beta Serum ELISA Kit, High Sensitivity (Cat. No. 42410-1).

 

Specifications

CVs and Spike Recovery

Inter-Assay < 8%

Intra-Assay < 8 %

 

Average Spike Recovery: > 94%

Cross-reactivity

No cross-reactivity against

  • Human IFN-Alpha, IFN-Gamma, IFN-Kappa, IFN-Beta
  • Mouse IFN-Alpha, IFN-Lambda
  • Rat IFN-Alpha, IFN-Lambda, IFN-Beta
  • Feline IFN-Alpha
  • Pig IFN-Alpha
Synonyms Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta
Storage 2-8°C
Expiration Date One year from the date of manufacture
Shipping Condition Wet Ice

 

 

Materials Provided

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Beta Standard, 500,000 pg/ml
  • Dilution Buffer 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • TMB Substrate 
  • Stop Solution 

 

Additional Materials Required (Not Provided) 

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker
 

Tech Info & Data

Tips, Tools and Troubleshooting:

 

Background

 

Interferons (IFNs) are a group of cytokines which exhibit pleitropic activities that play major roles in both innate and adaptive immunity. Type 1 IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates.

 

IFN-β is primarily known for its antiviral activity, but also has anti-proliferative, anti-inflammatory and immune-modulatory effects. It plays a pivotal role in the protective response to many infections and diseases, but when over expressed or produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes. IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice.

Citations

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    References:

     

    1. Staehelin et al. (1981) Methods in Enzymology, Vol. 79. (Pestka, ed.), Academic Press, New York, 589-595.

    2. Balachandran et al. (2004) Nature, Vol. 432, 401-405.

    Documentation

    Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
    42400 Protocol (Full)

    42400 Protocol (Full)

    42400-1 CoA

    42400-1 Certificate of Analysis (CoA)

    42400-2 CoA

    42400-2 Certificate of Analysis (CoA)

    42400 SDS

    42400 Safety Data Sheet

    42400 Product Notice Standard Stock Change

    42400 Product Notice Standard Stock Change

    42400 Product Notice Standard Reformulation

    42400 Product Notice Standard Reformulation