Mouse IFN Beta ELISA Kit (TCM, Serum)

Product Features: 
  • Measures mouse IFN beta in tissue culture media (TCM) and mouse serum

Full Product Name: VeriKine Mouse Interferon Beta ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
42400-1

1 x 96-well plate

$725.00
42400-2

5 x 96-well plate

$2,695.00
$2,995.00

Specifications

Compatibility: Tissue Culture Media, Serum
ELISA Assay Range: 15.6 - 1000 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Mouse Interferon Beta (No cross-reactivity against human IFN-α, IFN-γ, IFN-κ, IFN-β; mouse IFN-α, IFN-γ; rat IFN-α, IFN-γ, IFN-β; feline IFN-α; or pig IFN-α.)
Synonyms: Mouse Beta Interferon, Mouse IFN Beta, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Beta IFN, Mouse Type I Interferon Beta
 
Inter-Assay CV: < 8%
Intra-Assay CV: < 8%
Spike Recovery: > 94%
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Mouse Interferon Beta Standard, 500,000 pg/ml
  • Dilution Buffer 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate 
  • Stop Solution 

Tech Info / Data

Tips, Tools and Troubleshooting:

Detailed Related Product Information:

Background: 

The VeriKine Mouse Interferon Beta ELISA Kit has been developed to quantitate levels of IFN-β in tissue culture media or serum samples in a sandwich enzyme linked immunosorbent assay (ELISA) format with recombinant mouse IFN-beta expressed in mammalian cells provided as the standard in the kit. Interferons bind to plates coated with antibody and detection is accomplished using a secondary antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate.


Interferons (IFNs) are a group of cytokines which exhibit pleitropic activities that play major roles in both innate and adaptive immunity. Type 1 IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates.

IFN-β is primarily known for its antiviral activity, but also has anti-proliferative, anti-inflammatory and immune-modulatory effects. It plays a pivotal role in the protective response to many infections and diseases, but when over expressed or produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes. IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice.

Citations

Typical Standard Curve from 15.6 to 1000 pg/ml for PBL's Mouse IFN Beta ELISA (42400)

 

Mouse IFN beta standard curve from 15.6 to 1000 pg/ml using PBL Mouse Interferon Beta ELISA (42400)

Assay Range: 15.6 - 1000 pg/ml

Related Products

Catalog No. Description Pack Size Price Quantity
42410-1 VeriKine-HS Mouse Interferon Beta Serum ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 0.94-60 pg/ml

1 x 96-well plate

$525.00
42410-2 VeriKine-HS Mouse Interferon Beta Serum ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 0.94-60 pg/ml

5 x 96-well plate

$1,995.00
12405-1 Mouse Interferon Beta, mammalian  new 

1 x 105 units

$260.00
12400-1 Mouse Interferon Beta

1 x 105 units

$495.00

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