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Human IFN-Beta (Beta 1a, Mammalian Expressed)

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Human IFN-Beta (Beta 1a, Mammalian Expressed)

Catalog Number: 11415

Recombinant Human IFN-Beta 1a, mammalian, protein expressed in CHO cells is useful for MS research studies.

$370.00

Pack Size
Product Info

Supplied frozen with a carrier protein for retention of full activity.

Specifications

Formulation50 mM NaOAc, pH 5.5 containing 0.1% BSA
Molecular Weight~ 23 kDa
SourcecDNA obtained from human fibroblast interferon beta 1a mRNA expressed in CHO cells
Purity> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
BioactivityMeasured using a cytopathic inhibition assay on human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV). EC50 for IFN beta 1a in this assay is ~ 1 U/ml
StorageFor retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
SynonymsHuman IFNB, Human Beta Interferon, Human IFN Beta 1a, Human Interferon beta 1a, Human Type I Interferon Beta, Human IFN B
Accession NumberV00534

Citations

31 Citations

 

  1. Lee, A. & Nathan. C, (2024), "Type I interferon exacerbates Mycobacterium tuberculosis induced human macrophage death", EMBO Rep. PMID: 38866980, DOI: 10.1038/s44319-024-00171-0 (link)
  2. Eisfeld, A.J. et al., (2024), "A compendium of multi-omics data illuminating host responses to lethal human virus infections", Sci Data., 11(1):328, PMID: 38565538, DOI: 10.1038/s41597-024-03124-3 (link)
  3. Mandatori, S, et al.,  (2023), "PPKAG2.2 is essential for FoxA1+ regulatory T cell differentiation and metabolic rewiring distinct from FoxP3+ regulatory T cells", Sci Adv. 9(51):eadj8442, PMID: 38117896, DOI: 10.1126/sciadv.adj8442 (link)
  4. Lai, JH. et al., (2023), "USP18 enhances dengue virus replication by regulating mitochindrial DNA release", Sci Rep. 13(1):20126, PMID: 37978268, DOI: 10.1038/s41598-023-47584-w (link)
  5. Lin, C. et al., (2023), "Regulation of STING activity in DNA sensing by ISG15 modification", Cell Rep, 42(11):113277, PMID: 37864791, DOI: 10.1016/j.celrep.2023.113277 (link)
  6. Smith, J.R. et al., (2023), "MEF2A suppresses stress responses that trigger DDX41-dependent IFN production", Cell Rep., 42(8):112805, PMID: 37467105, DOI: 10.1016/j.celrep.2023.112805 (link)
  7. Sato, Y. et al., (2022), "Epstein-Barr virus tegument protein BGLF2 in exosomes released from virus-producing cells facilitates de novo infection", Cell Commun. Signal., 20(1):95, PMID: 35729616, DOI: 10.1186/s12964-022-00902-7 (link)
  8. Timilsina, U. et al., (2022), "SARS-CoV-2 ORF7a potently inhibits the antiviral effect of the host factor SERINC5", Nat. Commun., 13(1):2935, PMID: 35618710, DOI: 10.1038/s41467-022-30690-9 (link)
  9. Besson, B. et al., (2022), "Optimization of BRET saturation assays for robust and sensitive cytosolic protein-protein interaction studies", Scientific Reports, 12(1):9987, PMID: 35705637, DOI: 10.1038/s41598-022-12851-9 (link)
  10. Campbell, T. G. et al.,  (2022), "Respiratory viral infections in otherwise healthy humans with inherited IRF7 deficiency", JEM, 219(7):e20220202, PMID:35670811, DOI: 10.1084/jem.20220202 (link)
  11. Fung, S. K., et al.,(2022), "SARS-CoV-2 NSP13 helicase suppresses interferon signaling by perturbing JAK1 phosphorylation of STAT1", Cell & Bioscience, 12:36, DOI: 10.1186/s13578-022-00770-1, PMID: 35317858, (link)
  12. Abt, E.R., et al., (2022), "Reprogramming of nucleotide metabolism by interferon confers dependence on the replication stress response pathway in pancreatic cancer cells", Cell Reports, 38:110236, DOI: 10.1016/j.celrep.2021.110236 (link)
  13. Lama, et al. (2019). Development of human cGAS-specific small-molecule inhibitors for repression of dsDNA-triggered interferon expression. Nature Communications, 14 pgs. PMID: 31113940. (link)
  14. Cheng, Quen, et al. (2019). Sequential conditioning-stimulation reveals distinct gene- and stimulus-specific effects of Type I and II IFN on human macrophage functions. Scientific Reports, 14 pgs. PMID: 30918279. (link)
  15. Subramaniam, Meena (2019). Implementing and Applying Multiplexed Single Cell RNA-sequencing to Reveal Context- specific Effects in Systemic Lupus Erythematosus. UCSF, 85 pgs. PMID: no PMID.
  16. Gannon, et al. (2018). Identification of ADAR1 adenosine deaminase dependency in a subset of cancer cells. Nature Communications, 10 pgs. PMID: 30575730. (link)
  17. Zimmerman, Matthew, et al. (2018). Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host & Microbe, 28 pgs. PMID: 30439342. (link)
  18. Mariani, Melissa, et al. (2018). RNASeq Analysis Identifies Non-Canonical Role of STAT2 and IRF9 in the Regulation of a STAT1-Independent Antiviral and Immunoregulatory Transcriptional Program Induced by IFN-Beta and TNF-Alpha. BioRxiv, 23 pgs. PMID: no PMID. (link)
  19. Lau, Alexander, et al. (2017). Prevalence of Neutralising Antibodies to interferon-Beta and Clinical Response in Chinese Patients with Relapsing Multiple Sclerosis. MSJ, 10 pgs. PMID: 29051830. (link)
  20. Gram, Anna, et al. (2017). Human B Cells Fail to Secrete Type I Interferons Upon Cytoplasmic DNA Exposure. Molecular Immunology, 13 pgs. PMID: 28968560. (link)
  21. Kim, Ji-Ae, et al. (2016). Enhanced Viral Replication by Cellular Replicative Senescence. Immune Network, 10 pgs. PMID: 27799874. (link)
  22. Huang, Li-Yun, et al. (2016). Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5. PLOS One, 16 pgs. PMID: 27504984. (link)
  23. Gao, Xioafei, et al. (2016). Citrobacter rodentium NleB Inhibits Tumor Necrosis Factor (TNF) Receptor-Associated Factor 3 (TRAF3) Ubiquitination to Reduce Host Type I Interferon Production. JBC, 15 pgs. PMID: 27387501. (link)
  24. Rasouli, Javad, et al. (2015). Expression of GM-CSF in T Cells Is Increased in Multiple Sclerosis and Suppressed by IFN-β Therapy. Journal of Immunology, 20 pgs. PMID: 25917097. (link)
  25. Li, Yanfang, et al. (2014). A 20-amino-acid deletion in the neuraminidase stalk and a five-amino-acid deletion in the NS1 protein both contribute to the pathogenicity of H5N1 avian influenza viruses in mallard ducks. PLOS One, 12 pgs. PMID: 24743258. (link)
  26. Drewes, Julia, et al. (2014). Attenuation of Pathogenic Immune Responses During Infection with Human and Simian Immunodeficiency Virus (HIV/SIV) by the Tetracycline Derivative Minocycline. PLOS One, 15 pgs. PMID: 24732038. (link)
  27. Bolen, Christopher, et al. (2013). Dynamic Expression Profiling of Type I and Type III Interferon-Stimulated Hepatocytes Reveals a Stable Hierarchy of Gene Expression. Hepatology, 11 pgs. PMID: 23929627. (link)
  28. Salvi, et al. (2013). Dual regulation of osteopontin production by TLR stimulation in dendritic cells. JLB. PMID: 23610145. (link)
  29. Wang, Bei, et al. (2013). Enterovirus 71 protease 2Apro targets MAVS to inhibit anti-viral type I interferon responses. PLOS Pathogens, 18 pgs. PMID: 23555247. (link)
  30. Popugaeva, Elena, et al. (2012). Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLOS One, 10 pgs. PMID: 22545121. (link)
  31. Witte, et al. (2009). Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines. Nature, 13 pgs. PMID: 19798076. (link)

Documentation

Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
11415-1 CoA

11415-1 Certificate of Analysis (CoA)

11415 SDS

11415 Safety Data Sheet