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Human IFN-Beta ELISA Kit

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Human IFN-Beta ELISA Kit

Catalog Number: 41410

This well-cited ELISA quantitates IFN-Beta in tissue culture media (TCM) with an LLOQ of 50 pg/ml. Recombinant Human IFN-Beta 1a expressed in mammalian cells is provided as the standard.

 

NOTE: There has been a formulation change to the IFN-Beta Standard, please see the Product Notice located under the Documentation tab below for more information.

$615.00

Pack Size
Product Info

Matrix Compatibility Tissue Culture Media
Assay Range 50 - 4000 pg/ml
Assay Length 3 hours, 15 minutes
Specificity Human IFN-Beta

 

The VeriKine Human IFN-Beta ELISA Kit has been developed to measure levels of Human IFN-Beta in buffers or tissue culture media (TCM) samples by sandwich enzyme-linked immunosorbent assay (ELISA). The kit is based on an ELISA with streptavidin conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for Human IFN-Beta provided by the National Institutes of Health.

 

*For a more sensitive TCM-compatible ELISA, we recommend our Human IFN-Beta ELISA Kit, High Sensitivity (Cat. No. 41435-1).

 

*For complex biological matrices such as plasma, healthy sera, and disease sera, we recommend our Human IFN-Beta Serum ELISA Kit, High Sensitivity (Cat. No. 41415-1).

Specifications

CVs

Inter-Assay: ≤ 8%


Intra-Assay: ≤ 8%

Cross-reactivity

No cross-reactivity against

  • Human IFN-Alpha, IFN-Gamma
  • Mouse or rat IFN-Beta
Synonyms Human IFNB, Human Beta Interferon, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B
Storage 2-8°C
Expiration Date One year from date of manufacture
Shipping Condition Wet Ice

 

 

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN-Beta Standard, 100,000 pg/ml
  • Sample Diluent 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate Solution
  • Stop Solution

 

Additional Materials Required (Not Provided)

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data

Background

Following secretion, Interferon Beta binds to type I interferon receptors on proximal or distal cells activating the JAK1-STAT signaling pathway. Activation of this signal transduction pathway leads to the expression of 2’-5’ oligoadenylate synthetases (2’5’ OAS), protein kinase R (PKR), MxA proteins, and interferon regulatory factor 7 (IRF-7). The upregulation of IRF-7 expression can exert positive feedback on IFN-β production, whereas the induction of 2’5’ OAS activates a latent endonuclease known as RNase L. RNase L cleaves both viral and cellular single-stranded mRNA, thereby limiting viral replication and dissemination.

IFN-beta is present as a single gene in humans. The biological activity of IFN-beta can be functionally similar when compared to other type I IFNs, but can also exhibit distinct gene expression patterns as compared to IFN-alpha. In vitro binding studies suggest that IFN-beta binds to the Type I IFN receptor with higher affinity than the other Type I IFNs and this may regulate receptor internalization in a different manner. IFN-β has long been known to inhibit viral replication as part of the body’s innate antiviral response and it is currently used as a therapeutic for treatment of relapsing-remittent multiple sclerosis and some tumors.

Citations

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References:

  1. Staehelin et al. (1981) Methods in Enzymology, Vol. 79 (Pestka, ed.), Academic Press, New York, 589-595.

  2. Kelder et al. (1986) Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 582-587.

  3. Human IFN-β international reference standard provided by the NIH, reference no. Gb23-902-531. Pestka (1986) Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 14-23.

  4. Moschera et al. (1981) Methods of Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 177-183.

Documentation

Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
41410 Protocol (Full)

41410 Protocol (Full)

41410-1 CoA

41410-1 Certificate of Analysis (CoA)

42410-2 CoA

42410-2 Certificate of Analysis (CoA)

41410 SDS

41410 Safety Data Sheet

41410 Product Notice Standard Stock Change

41410 Product Notice Standard Stock Change

41410 Product Notice Standard Reformulation

41410 Product Notice Standard Reformulation