Human IFN Beta ELISA Kit

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Product Features: 
  • Quantifies human IFN beta in tissue culture media (TCM)
  • Reproducible results with inter-assay CV < 8% and intra-assay CV < 8%
  • Spike recovery > 90%
  • Recombinant Human IFN beta 1a expressed in mammalian cells provided as the standard

NOTE: There has been a formulation change to the Hu IFN Beta Standard, please see the Product Notice located under the Documentation tab below for more information.

 

Full Product Name: VeriKine Human Interferon Beta ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
41410-1

1 x 96-well plate

 $685.00
41410-2

5 x 96-well plate

 $2,595.00
$2,850.00

Specifications

Compatibility: Tissue Culture Media
Assay Range: 50 - 4000 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Beta
No cross-reactivity against human IFN-α, human IFN-γ, mouse IFN-β or rat IFN-β.
Synonyms: Human IFNB, Human Beta Interferon, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B
 
Inter-Assay CV: ≤ 8%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 90% in Serum
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Beta Standard, 100,000 pg/ml
  • Sample Diluent 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Background: 

The VeriKine Human Interferon Beta ELISA Kit has been developed to measure levels of human IFN-β in buffers or tissue culture media (TCM) samples by sandwich enzyme linked immunosorbent assay (ELISA). It is developed for superior performance with intra-assay and inter-assay CVs of ≤ 8%. The lowest concentration of Hu-IFN-β that can be detected in a test sample is 50 pg/ml. This kit is not designed to measure Hu-IFN-β in human serum. The kit is based on an ELISA with streptavidin conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon beta (Hu-IFN-β) provided by the National Institutes of Health.

IFN-beta is synthesized and secreted by fibroblasts and many other cell types in response to varied stimuli in Toll-like receptor (TLR) dependent and independent mechanisms. Pathogen exposure can result in the activation of transcription factors interferon regulatory factor 3 (IRF3) and NF-kB resulting in IFN beta production in a stimuli and cell dependent manner. Following secretion, IFN-β binds to type I interferon receptors on proximal or distal cells activating the JAK1-STAT signaling pathway. Activation of this signal transduction pathway leads to the expression of 2’-5’ oligoadenylate synthetases (2’5’ OAS), protein kinase R (PKR), MxA proteins, and interferon regulatory factor 7 (IRF-7). The upregulation of IRF-7 expression can exert a positive feedback on IFN-β production, whereas the induction of 2’5’ OAS activates a latent endonuclease known as RNase L. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.

IFN-beta is present as a single gene in humans. The biological activity of IFN-beta can be functionally similar when compared to other type I IFNs, but can also exhibit distinct gene expression patterns as compared to IFN-alpha. In vitro binding studies suggest that IFN-beta binds to the Type I IFN receptor with higher affinity than the other Type I IFNs and this may regulate receptor internalization in a different manner. IFN-β has long been known to inhibit viral replication as part of the body’s innate antiviral response and it is currently used as a therapeutic for treatment of relapsing-remittent multiple sclerosis and some tumors.

Citations

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References:

  1. Staehelin et al. (1981) Methods in Enzymology, Vol. 79 (Pestka, ed.), Academic Press, New York, 589-595.

  2. Kelder et al. (1986) Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 582-587.

  3. Human IFN-β international reference standard provided by the NIH, reference no. Gb23-902-531. Pestka (1986) Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 14-23.

  4. Moschera et al. (1981) Methods of Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 177-183.

Documentation

Typical Human IFN Beta standard curve from 50 to 4000 pg/ml using PBL Human IFN Beta ELISA kit (41410)

Typical Human IFN Beta standard curve from 50 to 4000 pg/ml using PBL Human IFN Beta ELISA (41410)

Assay Range: 50 - 4000 pg/ml

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