Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, Plasma, TCM)

Home / Products / Assay Kits / ELISAs / Human Interferon ELISAs / Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, Plasma, TCM)
Product Features: 
  • Human IFN Alpha ELISA quantitates low level human interferon alpha in normal & autoimmune sera, plasma, and TCM with LLOQ 1.95 pg/ml
  • Detects All 12 human IFN Alpha subtypes for accurate total IFN alpha measurement in samples
  • Reproducible inter- (<10%) and intra-assay (< 8%) CVs in all tested matrices provide reliable data

Full Product Name: VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit

 

Looking for a TCM compatible All Subtype ELISA? Check out our NEW 41135 kit!

Ordering

Catalog No. Pack Size Price Quantity
41115-1

1 x 96-well plate

 $695.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 1.95 - 125 pg/ml
Speed: Typical Incubation time, 22 hours, 30 minutes
Specificity: Human Interferon Alpha
Cross-reacts with Cynomolgus/Rhesus IFN-α. No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; or bovine IFN-τ.
Synonyms: IFN alpha, IFN-a, Multisubtype Interferon Alpha, Multisubtype Leukocyte Interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha

 

Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 80% in Serum

 

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Sample Buffer
  • Standard Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Antibody Diluent
  • HRP Diluent
  • TMB Substrate
  • Stop Solution

Tech Info / Data

IFN alpha-specific signals in 15 autoimmune sera using PBL High Sensitivity Human Interferon Alpha All Subtype ELISA (41115)
Background: 

This high sensitivity Human IFN Alpha ELISA kit is designed to provide accurate low pg/ml measurement of ALL human interferon alpha subtypes in autoimmune sera, normal serum, EDTA plasma, and tissue culture media (TCM). This unique feature provides an improved view of global IFN-alpha production in sample analysis. 

Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory and many other activities.

 

In humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence. Numerous individual human IFN-α subtypes have been identified; many display different properties. It remains unclear why there are multiple IFN-α subtypes. A variety of studies suggested they possess overlapping but also unique sets of biological activities.

Citations

12 Citations:

  1. Edahiro, Y., Ohishi, K., Gotoh, A. et al., (2022),  "Efficacy and safety of ropeginterferon alfa-2b in Japanese patients with polycythemia vera: an open-label, single-arm, phase 2 study", Int. J. Hematol., PMID: 35430707, DOI: 10.1007/s12185-022-03341-9  (link)
  2. Kanazawa, N. et al., (2021), Heterozygous missense variant of the proteasome subunit β-type 9 causes neonatal-onset autoinflammation and immunodeficiency, Nature Communications, 12:6819, DOI: 10.1038/s41467-021-27085-y (link)
  3. Jablonska, A., et al.,  (2021), The TLR9 2848C/T Polymorphism Is Associated with the CMV DNAemia among HIV/CMV Co-Infected Patients, Cells, 10:2360, DOI: 10.3390/cells10092360, (link)
  4. Peluso, Michael, et al. (2020). Liver function test abnormalities in a longitudinal cohort of Thai individuals treated since acute HIV infection. PLOS Pathogens, 9 pgs. PMID: 31953919. (link)

  5. Dagenais-Lussier, Xavier, et al. (2019). USP18 is a significant driver of memory CD4 T-cell reduced viability caused by type I IFN signaling during primary HIV-1 infection. PLOS Pathogens, 32 pgs. PMID: 31658294. (link)

  6. Colavita, Francesca, et al. (2018). Overproduction of IL-6 and Type-I IFN in a Lethal Case of Chikungunya Virus Infection in an Elderly Man During the 2017 Italian Outbreak. Open Forum Infectious Diseases, 21 pgs. PMID: 30539034. (link)

  7. Williams, Dionna, et al. (2018). CCR2 Signaling Selectively Regulates IFN-alpha: Role of Beta-Arrestin 2 in IFNAR1 Internalization. Journal of Immunology, 19 pgs. PMID: 30504423. (link)

  8. Zhang, Guoliang, et al. (2018). A Proline Deletion in IFNAR1 Impairs IFN-Signaling and Underlies Increased Resistance to Tuberculosis in Humans. Nature Communications, 9 pgs. PMID: 29311663. (link)

  9. Murayama, Goh, et al. (2017). Enhanced IFN-alpha Production is Associated with Increased TLR7 Retention in the Lysosomes of Palasmacytoid Dendritic Cells in Systemic Lupus Erythematosus. Arthritis Research & Therapy, 11 pgs. PMID: 29052537. (link)

  10. Wither, Joan, et al. (2017). Presence of an Interferon Signature in Individuals Who are Anti-Nuclear Antibody Positive Lacking a Systemic Autoimmune Rheumatic Disease Diagnosis. Arthritis Research & Therapy, 11 pgs. PMID: 28245862. (link)

  11. Vanheule, Vincent, et al. (2016). Basic Chemokine-Derived Glycosaminoglycan Binding Peptides Exert Antiviral Properties Against Dengue Virus Serotype 2, Herpes Simplex Virus-1, and Respiratory Syncytial Virus. Biochemical Pharmacology, 13 pgs. PMID: 26551597. (link)

  12. Combes, Alexis, et al. (2020). Global Absence and Targeting of Protective Immune States in Severe COVID-19. Nature, 36 pgs. (link)

References:

  1. Staehelin et al. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies" in Methods in Enzymology, Vol. 79 (Pestka, ed.), Academic Press, New York, 589-595.

  2. Kelder et al. (1986) "A Sandwich Radioimmunoassay for Human IFNa" in Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 582-587.

  3. Human IFN-α international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka (1986) "Interferon Standards and General Abbreviations" in Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 14-23.

  4. Rubinstein et al. (1981) "Human Leukocyte Interferon: Isolation and Characterization of Several Molecular Forms," Arch. Biochem. Biophys. 210, 307-318.

  5. Hobbs et al. (1982) "Purification and Characterization of Interferons from a Continuous Myeloblastic Cell Line," J. Biol. Chem. 257, 4071-4076.

Documentation

Representative Lower Limits of Quantitation and Lower Limits of Detection (pg/ml) in Normal Human Serum for each Human Interferon Alpha Subtype using PBL's High Sensitivity Human IFN Alpha ELISA (41115)

 

Representative LLOQs and LLODs in pg/ml in normal serum for all 12 human IFN alpha subtypes using HS IFN Alpha ELISA (41115)

Lower Limit of Quantitation (LLOQ) and Lower Limit of Detection (LLOD) for 41115

Related Products

Catalog No. Description Pack Size Price Quantity
41415-1 VeriKine-HS Human Interferon Beta Serum ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 1.2-150 pg/ml

1 x 96-well plate

 $725.00
41135-1 VeriKine-HS Human Interferon Alpha All Subtype TCM ELISA Kit
Compatibility: Tissue Culture Media
Assay Range: 1.95-125 pg/ml

1 x 96-well plate

 $445.00
41435-1 VeriKine-HS Human IFN Beta TCM ELISA Kit  new 
Compatibility: Tissue Culture Media
Assay Range: 2.34 - 150 pg/ml

1 x 96-well plate

 $485.00

Looking for a CoA?

Find your lot-specific Certificate of Analysis here!

Which service best suits your needs?

Finding the right biomarker detection service to meet your project needs just got easier...

Want to stay in touch?

Sign up for our email list to be kept up-to-date on the latest product releases and company news!

Go to top