Human IFNGR1 ELISA Kit (Serum, Plasma, Cell Lysate, TCM)
- Accurate quantitation of soluble and membrane Interferon Gamma Receptor 1
- Compatible with serum, plasma, cell lysates and tissue culture media (TCM)
- Provides high sensitivity detection of Human IFNGR1 to < 10 pg/ml in Sample Diluent and Cell Lysates
- < 4 hour assay time
- Conserve high value specimens with low sample requirements
Product Name: VeriKine Human Interferon-Gamma Receptor 1 ELISA Kit
$795.00
Product Info
| Compatibility | Serum, Plasma, Cell Lysate, Tissue Culture Media |
|---|---|
| Assay Range | 9.38 - 300 pg/ml (Sample Diluent and Cell Lysates), 234.38 - 7500 pg/ml (Serum and Plasma), 37.5 - 1200 pg/ml (TCM) |
| Assay Length | 3 hours, 15 minutes |
| Specificity | Human IFN-Gamma Receptor 1 (IFNGR1) |
| CV |
Inter-Assay: ≤ 12%
|
The VeriKine Human IFN-Gamma Receptor 1 ELISA quantitates Human IFN-Gamma Receptor 1 (IFNGR1) in serum, plasma, tissue culture media (TCM) and cell lysates by sandwich enzyme linked immunosorbent assay (ELISA). IFNGR1 binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. The standard provided is recombinant Human IFNGR1 expressed in mammalian cells.
Specifications
| Cross-reactivity |
No measurable IFNGR1 was observed when using this kit with sera of cynomolgus, rhesus, pig, bovine, rabbit, mouse, sheep or donkey.
No cross-reactivity against
|
|---|---|
| Storage | 2-8°C |
| Expiration Date | One year from date of manufacture |
| Storage | Wet Ice |
Materials Provided:
- Pre-coated microtiter plate
- Plate Sealers
- Wash Solution Concentrate
- Human IFN-Gamma Receptor 1 (IFNGR1) Standard, 500,000 pg/ml
- Sample Diluent
- Antibody Concentrate
- Antibody Diluent
- HRP Conjugate Concentrate
- HRP Diluent
- TMB Substrate
- Stop Solution
Citations
References: (For additional references, please reference the protocol.)
- Bach EA, Aguet M, and Schreiber RD. "The IFN-g receptor: a paradigm for cytokine receptor signaling." Annu. Rev. Immunol, 1997, 15: 563.
- Hawkyard SJ, et al. "The expression of interferon-g receptors on bladder cancer cells: does it correlate with biological response?" Urological Research, 1992, 20: 229-232.
- Merlin G, Falcoff E, and Aguet M. I-labelled Human Interferon Alpha, Beta and Gamma: Comparative Receptor-binding Data." J. Gen. Virol., 1985, 66: 1149-1152.
- Bello I, et al. High levels of soluble IFN gamma receptor alpha chain in the plasma of rheumatoid arthritis patients. Biotherapy, 1998, 11(1): 53-57.
- Toren A, et al. "Soluble interferon-gamma receptor and interferon-gamma in patients undergoing allogeneic bone marrow transplantation for hematological malignancies." Cytokines Cell Mol Ther., 1997, 3(3): 153-158.