Human IFNGR1 ELISA Kit (Serum, Plasma, Cell Lysate, TCM)

Product Features: 
  • Able to quantify soluble and membrane IFNGR1
  • Compatible with serumplasma, cell lysates and tissue culture media (TCM)
  • Provides high sensitivity detection of Human IFNGR1 to < 10 pg/ml in Sample Diluent and Cell Lysates
  • < 4 hour assay time
  • Conserve high value specimens with low sample requirements

Full Product Name: VeriKine Human Interferon Gamma Receptor 1 ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
41580-1

1 x 96-well plate

$775.00

Specifications

Compatibility: Serum, Plasma, Cell Lysate, Tissue Culture Media
Assay Range: 9.38 - 300 pg/ml (Sample Diluent and Cell Lysates), 234.38 - 7500 pg/ml (Serum and Plasma), 37.5 - 1200 pg/ml (TCM)
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Gamma Receptor 1 (IFNGR1)
No measurable IFNGR1 was observed when using this kit with sera of cynomolgus, rhesus, pig, bovine, rabbit, mouse, sheep or donkey. No cross-reactivity was detected with mouse IFNGR1-Fc.
 
Inter-Assay CV: ≤ 12%
Intra-Assay CV: ≤ 8%
Spike Recovery: 93%
 
Storage: 2-8°C
Expiration Date: 1 year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN Gamma Receptor 1 (IFNGR1) Standard, 500,000 pg/ml
  • Sample Diluent
  • Antibody Concentrate
  • Antibody Diluent 
  • HRP Conjugate Concentrate 
  • HRP Diluent
  • TMB Substrate 
  • Stop Solution 

Tech Info / Data

Background: 

The VeriKine Human IFN Gamma Receptor 1 ELISA quantitates Human IFN Gamma Receptor 1 (IFNGR1)  in serum, plasma, tissue culture media (TCM) and cell lysates by sandwich enzyme linked immunosorbent assay (ELISA). IFNGR1 binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. The standard provided is recombinant Human IFNGR1 expressed in mammalian cells.


The receptor for interferon gamma (IFN-gamma) is a membrane bound heterodimer of the IFNGR1 and IFNGR2 chain with IFNGR1 providing the high affinity binding site. IFNGR1 is also known as CD119 and Interferon Gamma Receptor alpha (IFNGRa). Binding of IFN-gamma to the receptor leads to activation of a number of genes and multiple biological activities. The receptor density can vary from 1,000 - 40,000 copies per cell depending upon the cell line. 

IFNGR1 can be present in two forms, the classical membrane bound form and a soluble form. Unlike IFNAR2 no alternatively spliced secreted transcript has been identified for IFNGR1 but is thought to be cleaved at the membrane by proteases in a manner similar to other cytokine receptors. There appears to be a basal level in the serum of healthy donors. The soluble IFNGR1 may be elevated in certain disease states such as Rheumatoid Arthritis and Graft vs. Host Disease.

 

Citations

Typical Standard Curve in Sample Diluent, Serum and TCM

Typical Standard Curve of 41580

Note: For serum curve, endogenous levels have been subtracted.

Related Products

Catalog No. Description Pack Size Price Quantity
41500-1 VeriKine Human Interferon Gamma ELISA Kit
Compatibility: Tissue Culture Media
Assay Range: 12.5-500 pg/ml

1 x 96-well plate

$350.00

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