Human IL-22 ELISA, High Sensitivity (Serum, Plasma, TCM)

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Product Features: 
  • Human IL-22 ELISA designed for accurate quantitation of endogenous human interleukin 22 in healthy & diseased serum/plasma, and tissue culture media samples
  • Assay LLOQ = 0.78 pg/ml
  • Measures unbound (free) IL-22. IL-22 Binding Protein (IL-22BP) may interfere with the assay if present at high concentration
  • Highly reproducible results in all tested matrices with inter- and intra-CVs < 10%

 

Full product name: VeriKine-HS Human Interleukin 22 ELISA Kit

 

Ordering

Catalog No. Pack Size Price Quantity
41701-1

1 x 96-well plate

 $575.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.78 - 50 pg/ml
Assay Length: 4 hours
Specificity: Human Interleukin 22
Cross-reacts with Monkey IL-22 (< 0.03%).
No cross-reactivity detected with human IL-10, IL-17, IL-19, IL-20, IL-24, IL-26/AK155, IFN-β1a, IFN-γ, IFN-ω; cynomolgus IFN-α2; cynomolgus/rhesus IFN-α; mouse IL-22, IFN-αA, IFN-β, IFN-γ; rat IL-22, IFN-α1, IFN-α14, IFN-β, IFN-γ; bovine IFN-Tau2 or pig IFN-α.

 

Inter-Assay CV: < 10%
Intra-Assay CV: < 10%
Spike recovery: > 96%

 

Storage: 2-8 °C
Expiration Date: one year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-Coated Micro-Titer Stripwell Plate/Sealers
  • Wash Solution Concentrate
  • Human Interleukin-22 Standard (10,000 pg/ml)
  • Standard Diluent
  • Assay Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • HRP Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Representative Standard Curve for PBL's VeriKine-HS Human IL-22 ELISA Kit (Catalog No. 41701)
Human IL-22 ELISA Normal Human Serum (NHS) Parallelism using PBL's VeriKine-HS Human IL-22 ELISA (Catalog No. 41701)
Human IL-22 ELISA Disease Serum Parallelism using PBL's VeriKine-HS Human IL-22 ELISA (Catalog No. 41701)
PBL's Human IL-22 ELISA in the present of Human Interleukin 22 Binding Protein (IL-22BP)
Background: 

Interleukin 22 (IL-22) is a member of the IL-10 family of cytokines1. Other names include IL-TIF and Zcyto18. IL-22 is produced by a variety of cells including Th1, Th17, Th22 T-cells, NKT cells, ILC3, neutrophils and macrophages2.  Targeting primarily non-hemopoietic cells such as stromal and epithelial cells, IL-22 exerts both protective effects, such as hepatocyte and epithelial cell survival, and pro-inflammatory effects. It is also implicated in autoimmune diseases such as Systemic Lupus Erythematosus, Rheumatoid Arthritis and Psoriasis3. IL-22 is often considered a hallmark of IL-17 driven immune responses4.

Citations

References:

  1. Dumoutier, L., et al. 2000. Genes & Immunity.  1(8): 488-94.

  2. Dudakov J., Hanash A., van den Brink, M. 2015. Annual Review of Immunology. 33: 747-785.

  3. Eyerich, K., Dimartino, V., Cavani, A. 2017. European Journal of Immunology. 47(4): 607-614.

  4. Eyerich, S., et al. 2017. Allergologie Select. 1(1): 71-76.

Documentation

High Sensitivity Human IL-22 ELISA exhibits high detectability of endogenous IL-22 in autoimmune/IL-22 related disease sera

 

High detectability of endogenous IL-22 in patient serum samples

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41702-1 VeriKine-HS Human Interleukin 15 ELISA Kit  new 
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