Human IL-22 ELISA, High Sensitivity (Serum, Plasma, TCM)

Home / Products / Assay Kits / ELISAs / Human Interleukin ELISAs / Human IL-22 ELISA, High Sensitivity (Serum, Plasma, TCM)
Product Features: 
  • Human IL-22 ELISA with very low picogram sensitivity (LLOQ 0.78 pg/ml) for human interleukin 22 in healthy and patient donor serum, plasma, and tissue culture media (TCM)
  • Accurate quantitation of endogenous human interleukin 22 in healthy and patient serum samples
  • Accurate measurement of unbound (free) IL-22. IL-22 Binding Protein (IL-22BP) may interfere with the assay if present at high concentration
  • Highly reproducible results in all tested matrices with inter- and intra-CVs < 10%

 

Full product name: VeriKine-HS Human Interleukin 22 ELISA Kit

 

Ordering

Catalog No. Pack Size Price Quantity
41701-1

1 x 96-well plate

 $575.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.78 - 50 pg/ml
Assay Length: 4 hours
Specificity: Human Interleukin 22
Cross-reacts with Monkey IL-22 (< 0.03%).
No cross-reactivity detected with human IL-10, IL-17, IL-19, IL-20, IL-24, IL-26/AK155, IFN-β1a, IFN-γ, IFN-ω; cynomolgus IFN-α2; cynomolgus/rhesus IFN-α; mouse IL-22, IFN-αA, IFN-β, IFN-γ; rat IL-22, IFN-α1, IFN-α14, IFN-β, IFN-γ; bovine IFN-Tau2 or pig IFN-α.

 

Inter-Assay CV: < 10%
Intra-Assay CV: < 10%
Spike recovery: > 96%

 

Storage: 2-8 °C
Expiration Date: one year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-Coated Micro-Titer Stripwell Plate/Sealers
  • Wash Solution Concentrate
  • Human Interleukin-22 Standard (10,000 pg/ml)
  • Standard Diluent
  • Assay Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • HRP Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Representative Standard Curve for PBL's VeriKine-HS Human IL-22 ELISA Kit (Catalog No. 41701)
Human IL-22 ELISA Normal Human Serum (NHS) Parallelism using PBL's VeriKine-HS Human IL-22 ELISA (Catalog No. 41701)
Human IL-22 ELISA Disease Serum Parallelism using PBL's VeriKine-HS Human IL-22 ELISA (Catalog No. 41701)
PBL's Human IL-22 ELISA in the present of Human Interleukin 22 Binding Protein (IL-22BP)
Background: 

Interleukin 22 (IL-22) is a member of the IL-10 family of cytokines1. Other names include IL-TIF and Zcyto18. IL-22 is produced by a variety of cells including Th1, Th17, Th22 T-cells, NKT cells, ILC3, neutrophils and macrophages2.  Targeting primarily non-hemopoietic cells such as stromal and epithelial cells, IL-22 exerts both protective effects, such as hepatocyte and epithelial cell survival, and pro-inflammatory effects. It is also implicated in autoimmune diseases such as Systemic Lupus Erythematosus, Rheumatoid Arthritis and Psoriasis3. IL-22 is often considered a hallmark of IL-17 driven immune responses4.

Citations

References:

  1. Dumoutier, L., et al. 2000. Genes & Immunity.  1(8): 488-94.

  2. Dudakov J., Hanash A., van den Brink, M. 2015. Annual Review of Immunology. 33: 747-785.

  3. Eyerich, K., Dimartino, V., Cavani, A. 2017. European Journal of Immunology. 47(4): 607-614.

  4. Eyerich, S., et al. 2017. Allergologie Select. 1(1): 71-76.

Documentation

High Sensitivity Human IL-22 ELISA exhibits high detectability of endogenous IL-22 in autoimmune/IL-22 related disease sera

 

High detectability of endogenous IL-22 in patient serum samples

Related Products

Catalog No. Description Pack Size Price Quantity
11701-1 Human Interleukin 22 (Human Cell-Expressed)  new 

10 µg

 $175.00
11701-2 Human Interleukin 22 (Human Cell-Expressed)  new 

50 µg

 $350.00
41702-1 VeriKine-HS Human Interleukin 15 ELISA Kit  new 
Compatibility: Serum, Plasma, and Tissue Culture Media (TCM)
Assay Range: 0.51 - 32.8 pg/ml

1 x 96-well plate

 $550.00

Looking for a CoA?

Find your lot-specific Certificate of Analysis here!

Which service best suits your needs?

Finding the right biomarker detection service to meet your project needs just got easier...

Want to stay in touch?

Sign up for our email list to be kept up-to-date on the latest product releases and company news!

Go to top