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Pig IFN-Alpha ELISA Kit (Serum, Plasma, TCM)

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Pig IFN-Alpha ELISA Kit (Serum, Plasma, TCM)

Catalog Number: 47100

This ELISA quantifies Porcine IFN-Alpha in serum, plasma, and tissue culture media (TCM) with an LLOQ of 2.34 pg/ml.

$610.00

Pack Size
Product Info

Matrix Compatibility Serum, Plasma, Cell Culture Supernatant
Assay Range 2.34 - 150 pg/ml
LLOQ

2.34 pg/ml

Need more sensitivity? Check out our Sample Testing Services

Assay Length 3 hours, 15 minutes
Specificity Pig Interferon Alpha

 

 

The Verikine-HS Pig IFN-Alpha ELISA kit will enable determination of IFN-Alpha levels in serum, plasma, and tissue culture media. As such, it should prove an important tool in virology, immunomodulation, and immunotoxicology studies conducted in pigs.

Specifications

CVs and Spike Recovery

Inter-Assay < 10%

Intra-Assay < 8 %

 

Average Spike Recovery: 107%

Cross-reactivity

No cross-reactivity against

  • Pig IFN-Beta, IFN-Gamma
  • Human IFN-Alpha, IFN-Beta, IFN-Gamma, IFN-Omega
  • Mouse IFN-Alpha, IFN-Beta, IFN-Lambda
  • Rat IFN-Alpha
Storage 2-8°C
Expiration Date One year from date of manufacture
Shipping Condition Wet Ice

 

 

Materials Provided

  • Pre-coated microtiter plate
  • Plate sealers
  • Wash Solution Concentrate
  • Pig IFN-Alpha Standard, 10,000 pg/ml
  • Dilution Buffer 
  • Assay Buffer
  • Antibody Concentrate 
  • Antibody Diluent
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate Solution
  • Stop Solution 

 

Additional Materials Required (Not Provided) 

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data

Background

 

Interferons (IFNs) are a group of cytokines which exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of at least one IFN-β gene and protein as well as multiple IFN-α genes and proteins in most vertebrate species. IFN-α expression and secretion is primarily induced by signaling events processed through pattern recognition receptors such as the Toll-like and RIG-I like receptors (TLR and RLR, respectively). While IFN-α can be produced by most cell types, strong evidence suggests that plasmacytoid dendritic cells are a major source of IFN-α in vivo. Following expression and secretion, IFN-α binds to a hetero-dimeric receptor chain consisting of IFNAR1 and IFNAR2 subunits on proximal and distal cell surfaces. Receptor binding promotes a signal transduction cascade consisting of components of the JAK-STAT signaling pathway. Hundreds of genes are regulated subsequent to binding of the IFNAR receptor subunits to IFN-α, thus leading to the antiviral, anti-proliferative, and immunomodulatory activities of the cytokine.

 

The domestic pig (Sus scrofa or Sus domestica) is an important livestock animal. As such, diseases that affect livestock are frequently studied in this animal. Furthermore, it is also a significant model of human infectious disease. One of the hallmarks of viral and other infectious diseases is the production of interferon. A number of studies have already highlighted the importance of the IFN system in pigs.

Citations

1 Citation:

 

  1. Vandoorn, E. et al., (2022), "Pathobiology of an NS1-Truncated H3N2 Swine Influenza Virus Strain in Pigs", J. Virolo., e0051922, PMID: 35546120, DOI: 10.1128/jvi.00519-22 (link)

 

References: 

 

  1. Krause CD, Pestka S. (2005) “Evolution of the Class 2 cytokines and receptors, and discovery of new friends and relatives,” Pharmacol Ther. 106(3):299-346.

  2. Fitzgerald-Bocarsly P, et al. (2008) “Plasmacytoid dendritic cells and type I IFN: 50 years of convergent history,” Cytokine Growth Factor Rev. 19(1):3-19.

  3. Davies PR. (2012) “One world, one health: the threat of emerging swine diseases. A North American perspective.” Transbound Emerg Dis. 59 Suppl 1:18-26.

  4. Meurens F, et al. “The pig: a model for human infectious diseases.” Trends Microbiol. 20(1):50-7.

  5. Schook L, et al. (2005) “Swine in biomedical research: creating the building blocks of animal models.” Anim Biotechnol. 16(2):183-90.

  6. Razzuoli E, et al. (2011) "Characterization of the interferon-α response of pigs to the weaning  stress." J. Interferon Cytokine Res. 31(2):237-47

  7. Souza M, et al. (2007) "Cytokine and antibody responses in gnotobiotic pigs after infection with human norovirus genogroup II.4 (HS66 Strain)." J. Virol., 81(17):9183-92

  8. Van Reeth et al., (1998) "Bronchoalveolar interferon-α Tumor Necrosis Factor-α, Interleukin-1, and Inflammation during Acute Influenza in Pigs: A Possible Model for Humans?" J. Infect. Dis. 177(4):1076-1079

Documentation

Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
47100-1 CoA & Protocol (One-Page)

47100-1 Certificate of Analysis (CoA) & Protocol (One-Page)

47100-1 Protocol (Full)

47100-1 Protocol (Full)

47100 SDS

47100 Safety Data Sheet

47100 Product Flyer

47100 Product Flyer