Pig IFN Alpha ELISA Kit (Serum, Plasma, TCM)

Product Features: 
  • Quantifies Porcine IFN-Alpha (Pig IFN-α) in serum, plasma, and tissue culture media (TCM)
  • Sensitive detection to as low as 2.34 pg/ml
  • High precision reproducibility & reliability with inter-assay CV ≤ 10% and intra-assay CV ≤ 8%

Full Product Name: VeriKine-HS Pig Interferon Alpha ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
47100-1

1 x 96-well plate

$595.00

Specifications

Compatibility: Serum, Plasma, Cell Culture Supernatant
Assay Range: 2.34 - 150 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Pig Interferon Alpha
No cross-reactivity was observed when using this kit to detect the following, each at up to 20 ng/ml - Pig: IFN-β, IFN-γ; Human: IFN-α, IFN-β, IFN-γ, IFN-ω; Mouse: IFN-α, IFN-β, IFN-γ; Rat: IFN-α
 
Inter-Assay CV: < 10%
Intra-Assay CV: <  8%
Spike Recovery: 107%
 
Storage: 2-8°C
Expiration Date: 12 months from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate sealers
  • Wash Solution Concentrate
  • Pig Interferon Alpha Standard, 10,000 pg/ml
  • Dilution Buffer 
  • Assay Buffer
  • Antibody Concentrate 
  • Antibody Diluent
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate Solution
  • Stop Solution 

Tech Info / Data

Background: 

The Verikine-HS Pig IFN-α ELISA kit will enable determination of IFN-α levels in serum, plasma, and tissue culture media. As such, it should prove an important tool in virology, immunomodulation, and immunotoxicology studies conducted in pigs.


Interferons (IFNs) are a group of cytokines which exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of at least one IFN-β gene and protein as well as multiple IFN-α genes and proteins in most vertebrate species. IFN-α expression and secretion is primarily induced by signaling events processed through pattern recognition receptors such as the Toll-like and RIG-I like receptors (TLR and RLR, respectively). While IFN-α can be produced by most cell types, strong evidence suggests that plasmacytoid dendritic cells are a major source of IFN-α in vivo. Following expression and secretion, IFN-α binds to a hetero-dimeric receptor chain consisting of IFNAR1 and IFNAR2 subunits on proximal and distal cell surfaces. Receptor binding promotes a signal transduction cascade consisting of components of the JAK-STAT signaling pathway. Hundreds of genes are regulated subsequent to binding of the IFNAR receptor subunits to IFN-α, thus leading to the antiviral, anti-proliferative, and immunomodulatory activities of the cytokine.

The domestic pig (Sus scrofa or Sus domestica) is an important livestock animal. As such, diseases that affect livestock are frequently studied in this animal. Furthermore, it is also a significant model of human infectious disease. One of the hallmarks of viral and other infectious diseases is the production of interferon. A number of studies have already highlighted the importance of the IFN system in pigs.

Citations

Typical Standard Curve

Pig IFN Alpha Typical Standard Curve (Porcine Interferon Alpha)

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