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Catalog No. | Pack Size | Price | Quantity |
---|---|---|---|
21900-1 |
100 μg |
$265.00 |
Formulation | 50 mM Tris HCl, 75 mM NaCl, 50% Glycerol pH 8 |
Antigen | Human ISG15 |
Isotype | Mouse IgG1 kappa |
Host Species | Mouse |
Purity | > 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
Specificity | Detects human ISG15 |
Storage | After receipt, this product should be kept at -20oC for retention of full activity. Do not store below -20oC. |
Clone | 2.1 |
Tested Applications:
Many published reports indicate that ISG15 (G1P2) is commonly upregulated in response to Type I interferon stimulation. Though a wide range of cellular activites are influenced by ISG15 expression including Type I interferon signaling, translation, chromatin remodeling, cell motility, protein trafficking, and protein conjugation (ISGylation), the complete spectrum of ISG15-dependent biological sequelae remains to be fully elucidated. Further definition of new ISG15 target proteins and the role of free ISG15 will offer new insights into mechanistic and immunotherapeutic approaches to human diseases.
References
Control (C) and Treated (T) 40 μg lysate samples from A549, Ovcar and HeLa cells were run alongside 50 ng recombinant ISG15 and 100 μg ubiquitin on a 12% SDS-PAGE gel under reducing conditions. Cells were either untreated (C) or treated (T) with 1000 U/ml of Human IFN beta 1a (PBL Cat. No. 11410-2) and lysed with 50 mM Tris-HCl pH8, 200 mM NaCl, 10% Glycerol, 0.5% NP-40, 0.1 mM EDTA with protease inhibitors on ice for one hour and then centrifuged at 4°C to obtain supernatants. No cross-reactivity was observed with ubiquitin at tested concentrations up to 100 μg. The antibody was additionally tested on Daudi and U937 cell lysates (data not shown).
*A549 FT: Lysate after one freeze-thaw cycle
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PBL Assay Science offers scientists a comprehensive range of assay kits, interferons, cytokines, growth factors and antibodies. Our high quality products provide consistent, reliable performance and are suitable for use in a range of research applications.
Quick Search
PBL’s improved site search function is defaulted to an exact match.
Search Term Results |
|
IFN beta |
All interferon beta products and related technical information (e.g. technical notes) |
Human IFN beta |
All human interferon products related technical information (e.g. technical notes) |
IL-2 |
All products contain the term “IL-2”, including IL-23 |
IL 2 |
All products contain the term “IL 2”, including IL-23 |
Erythropoietin |
All EPO products and technical information |
Eryth |
All EPO products and technical information |
The results are displayed in best match products grouped in the following product category filters:
Each filter contains sub-categories for further narrowing of your search results
Other web content such as technical notes that contain the Search Term can be displayed by checking the box next to the corresponding filter on the left side of the Search Results Page.