Anti-Human ISG15, Clone 2.1 (MAb)

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Product Features: 
  • Detects conjugated and free human ISG15
  • No cross-reactivity with 1000x load of ubiquitin on Western Blot
  • Greater than 95% purity

Ordering

Catalog No. Pack Size Price Quantity
21900-1

100 μg

 $295.00

Specifications

Formulation Lyophilized in PBS
Molecular Weight 22 kDa, monomer, non-glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 0.4 to 2 ng/ml
Purity 95% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

  1. 11619 Purity Confirmation on SDS-PAGE
Background:

Recombinant Human Growth Hormone (HGH), expressed in engineered human cells, is a member of the somatotropin/prolactin family of hormones. HGH is produced from the pituitary gland and is required for normal human growth and development. HGH modifies a variety of physiological functions by stimulating the expression of insulin-like growth factor I and increases calcium retention and bone mineralization. HGH increases muscle mass, promotes lipolysis and reduces liver uptake of glucose.

Citations

References:

  1. Gout, P. W., et al (1980) Cancer Research 40, 2433-2436.

  2. Goffin, V., et al (1996) Endocrine Reviews 17, 385-410.

Documentation

Western Blot

21900-1 Western Blot for Anti-Human ISG15

Control (C) and Treated (T) 40 μg lysate samples from A549, Ovcar and HeLa cells were run alongside 50 ng recombinant ISG15 and 100 μg ubiquitin on a 12% SDS-PAGE gel under reducing conditions. Cells were either untreated (C) or treated (T) with 1000 U/ml of Human IFN beta 1a (PBL Cat. No. 11410-2) and lysed with 50 mM Tris-HCl pH8, 200 mM NaCl, 10% Glycerol, 0.5% NP-40, 0.1 mM EDTA with protease inhibitors on ice for one hour and then centrifuged at 4°C to obtain supernatants. No cross-reactivity was observed with ubiquitin at tested concentrations up to 100 μg. The antibody was additionally tested on Daudi and U937 cell lysates (data not shown).

*A549 FT: Lysate after one freeze-thaw cycle

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