Human IL-15 ELISA (High Sensitivity)

Product Features: 
  • Measures endogenous human IL-15 at or near basal level with LLOQ of 0.51 pg/ml in serum, plasma, and cell culture media
  • Detects both free and bound form of IL-15 (IL-15/IL-15R complex)
  • Reproducible results with inter- and intra-CVs <10% in all tested matrices

 

Full product name: VeriKine-HS Human Interleukin 15 ELISA Kit

 

Ordering

Catalog No. Pack Size Price Quantity
41702-1

1 x 96-well plate

$550.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.51 - 32.8 pg/ml
Assay Length: 4 hours
Specificity: Human Interleukin 15

No cross-reactivity detected with human IL-2, IL-4, IL-7, IL-9, IL-21, IFN-α, IFN-β1a, IFN-γ, IFN-ω; cynomolgus IFN-α2; cynomolgus/rhesus IFN-α; mouse IL-15, IFN-αA, IFN-β, IFN-γ; rat IL-15, IFN-α1, IFN-α14, IFN-β, IFN-γ; bovine IFN-Tau2 or pig IFN-α.

Inter-Assay CV: < 10%
Intra-Assay CV: < 10%
Spike recovery: 103%

 

Storage: 2-8 °C
Expiration Date: one year from date of manufacture
Shipping Condition: Wet Ice


Materials Provided:

  • Pre-Coated Micro-Titer Stripwell Plate/Sealers
  • Wash Solution Concentrate
  • Human Interleukin-15 Standard (410 pg/ml)
  • Assay Buffer
  • Dilution Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • HRP Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Human IL-15 ELISA Normal Human Serum Linearity (NHS) using PBL
Human IL-15 ELISA Normal Human Serum Parallelism (NHS) using PBL
Human IL-15 ELISA Plasma Parallelism using PBL
Human IL-15 ELISA Normal Human Serum (NHS) and Plasma Knockout using PBL
Background: 

Interleukin-15 (IL-15) is a member of the IL-10 family of cytokines1. Other names include IL-TIF and Zcyto18. IL-15 is produced by a variety of cells including TH1, TH17, TH15 T-cells, NKT cells, ILC3, neutrophils and macrophages2. IL-15 targets primarily non-hemopoietic cells such as stromal and epithelial cells. IL-15 has both protective effects, such as hepatocyte and epithelial cell survival, and pro-inflammatory effects. It is also implicated in autoimmune diseases such as Systemic Lupus Erythematosus, Rheumatoid Arthritis and Psoriasis3. IL-15 is often considered a hallmark of IL-17 driven immune responses4.

 

PBL’s IL-15 ELISA measures both IL-15 alone and IL-15/IL15Rα complexes in Tissue Culture Media (TCM), and complex matrices such as serum and plasma. It can also quantitate IL-15 in healthy donor matrices. The standard in this assay is the IL-15/IL-15R heterodimer but because the antibodies used are specific for IL-15, the standard curve is calibrated to the IL-15 portion of the complex. Different forms of the IL-15 receptors (IL15-R) may couple to free IL-15 protein. This assay can detect both free IL-15 and bound IL-15/IL-15R.

 

Serum samples from 30 healthy donors and plasma samples from 47 healthy donors were all quantifiable and as such this assay may provide basal level measurement.

Citations

High Sensitivity Human IL-15 ELISA Exhibits Greater Sensitivity than Competitive IL-15 Standards

 

. Dose response curves and absorbance values of all IL-15 standards are shown below. PBL’s standard exhibited greater sensitivity than competitive IL-15 standards

Related Products

Catalog No. Description Pack Size Price Quantity
41701-1 VeriKine-HS Human Interleukin 22 ELISA Kit  new 
Compatibility: Serum, Plasma, and Tissue Culture Media (TCM)
Assay Range: 0.78 - 50 pg/ml

1 x 96-well plate

$575.00
11702-1 Human Interleukin 15 / sIL-15Rα (Human Cell-Expressed)  new 

10 µg

$195.00
11702-2 Human Interleukin 15 / sIL-15Rα (Human Cell-Expressed)  new 

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$450.00

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