Human IL-15 ELISA (High Sensitivity)

Product Features: 
  • Measures endogenous human IL-15 at or near basal level with LLOQ of 0.51 pg/ml in serum, plasma, and cell culture media
  • Detects both free and bound form of IL-15 (IL-15/IL-15R complex)
  • Reproducible results with inter- and intra-CVs <10% in all tested matrices


Full product name: VeriKine-HS Human Interleukin 15 ELISA Kit



Catalog No. Pack Size Price Quantity

1 x 96-well plate



Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 0.51 - 32.8 pg/ml
Assay Length: 4 hours
Specificity: Human Interleukin 15

No cross-reactivity detected with human IL-2, IL-4, IL-7, IL-9, IL-21, IFN-α, IFN-β1a, IFN-γ, IFN-ω; cynomolgus IFN-α2; cynomolgus/rhesus IFN-α; mouse IL-15, IFN-αA, IFN-β, IFN-γ; rat IL-15, IFN-α1, IFN-α14, IFN-β, IFN-γ; bovine IFN-Tau2 or pig IFN-α.

Inter-Assay CV: < 10%
Intra-Assay CV: < 10%
Spike recovery: 103%


Storage: 2-8 °C
Expiration Date: one year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-Coated Micro-Titer Stripwell Plate/Sealers
  • Wash Solution Concentrate
  • Human Interleukin-15 Standard (410 pg/ml)
  • Assay Buffer
  • Dilution Buffer
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • HRP Diluent
  • TMB Substrate Solution
  • Stop Solution

Tech Info / Data

Human IL-15 ELISA Normal Human Serum Linearity (NHS) using PBL
Human IL-15 ELISA Normal Human Serum Parallelism (NHS) using PBL
Human IL-15 ELISA Plasma Parallelism using PBL
Human IL-15 ELISA Normal Human Serum (NHS) and Plasma Knockout using PBL

Interleukin-15 (IL-15) is a member of the IL-10 family of cytokines1. Other names include IL-TIF and Zcyto18. IL-15 is produced by a variety of cells including TH1, TH17, TH15 T-cells, NKT cells, ILC3, neutrophils and macrophages2. IL-15 targets primarily non-hemopoietic cells such as stromal and epithelial cells. IL-15 has both protective effects, such as hepatocyte and epithelial cell survival, and pro-inflammatory effects. It is also implicated in autoimmune diseases such as Systemic Lupus Erythematosus, Rheumatoid Arthritis and Psoriasis3. IL-15 is often considered a hallmark of IL-17 driven immune responses4.


PBL’s IL-15 ELISA measures both IL-15 alone and IL-15/IL15Rα complexes in Tissue Culture Media (TCM), and complex matrices such as serum and plasma. It can also quantitate IL-15 in healthy donor matrices. The standard in this assay is the IL-15/IL-15R heterodimer but because the antibodies used are specific for IL-15, the standard curve is calibrated to the IL-15 portion of the complex. Different forms of the IL-15 receptors (IL15-R) may couple to free IL-15 protein. This assay can detect both free IL-15 and bound IL-15/IL-15R.


Serum samples from 30 healthy donors and plasma samples from 47 healthy donors were all quantifiable and as such this assay may provide basal level measurement.


High Sensitivity Human IL-15 ELISA Exhibits Greater Sensitivity than Competitive IL-15 Standards


. Dose response curves and absorbance values of all IL-15 standards are shown below. PBL’s standard exhibited greater sensitivity than competitive IL-15 standards

Related Products

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41701-1 VeriKine-HS Human Interleukin 22 ELISA Kit  new 
Compatibility: Serum, Plasma, and Tissue Culture Media (TCM)
Assay Range: 0.78 - 50 pg/ml

1 x 96-well plate

11702-1 Human Interleukin 15 / sIL-15Rα (Human Cell-Expressed)  new 

10 µg

11702-2 Human Interleukin 15 / sIL-15Rα (Human Cell-Expressed)  new 

50 µg


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