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Human IFN-Alpha A (Alpha 2a)

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Human IFN-Alpha A (Alpha 2a)

Catalog Number: 11100

Well-cited Human Interferon Alpha 2a recombinant protein with high activity. 

$335.00

Pack Size
Product Info

Recombinant Human Interferon Alpha A (Alpha 2a) obtained from human leukocyte mRNA expressed in E.coli.

 

IFN-Alpha 2a is one of the many interferon alpha subtypes belonging to the Type I leukocyte IFN family. The Type I IFN family has antiviral, anti-proliferative, and natural killer cell activities.

 

IFN-Alpha 2a and b are the most widely used therapeutic IFN-Alphas.

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.2 kDa
Source cDNA obtained from human leukocyte mRNA expressed in E.coli
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Bioactivity Measured using a cytopathic inhibition assay on Bovine Kidney Cells (MDBK) with Vesicular Stomatis Virus (VSV); human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV)
Storage For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles
Synonyms Interferon alpha A, IFN alpha A, IFNaA, IFN alpha 2a, IFN alpha A, IFN-a, interferon alpha, IFN-a2a, interferon alpha 2a, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, Type I interferon alpha, Type I IFN alpha
Accession Number V00549

Tech Info & Data

Application Note

 

  • A Comparison of the Binding of Interferon Alpha Subtypes to Isolated IFNAR1 and IFNAR2 with Activity in Antiviral and Antiproliferative Assays (link)

Citations

47 Citations

 

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  3. Alphonse, N. et al.,  (2022), "A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling", Cell, S0092-8674(22)00526-8, PMID: 35568036, DOI: 10.1016/j.cell.2022.04.028 (link)

  4. Tomg, S., et al., (2022), "Inhibition of interferon-gamma-stimulated melanoma progression by targeting neuronal nitric oxide synthase (nNOS)', Scientific Reports, 12:1701, DOI: 10.1038/s41598-022-05394-6 (link)

  5. Savvateeva, E. et al., (2021), "Microarray-Based Detection of Antibodies against SARS-CoV-2 Proteins, Common Respiratory Viruses and Type I Interferons", Viruses, 13:2553, DOI: 10.3390/v13122553 (link)

  6. Yang, Dong, et al. (2020). Attenuated interferon and pro-inflammatory response in SARS-CoV-2-infected human dendritic cells is associated with viral antagonism of STAT1 phosphorylation. The Journal of Infectious Diseases, 34 pgs. PMID: 32563187. (link)

  7. Kumar, Manickam, et al. (2020). In vitro replicative fitness of early Transmitted founder HIV-1 variants and sensitivity to Interferon alpha. Scientific Reports, 12 pgs. PMID: 32066770. (link)

  8. Sharma, Amit, et al. (2019). Macaque interferon-induced transmembrane proteins limit replication of SHIV strains in an Envelope-dependent manner. PLOS Pathogens, 23 pgs. PMID: 31260493. (link)

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  12. Couret, Jennifer, et al. (2017). Differential Regulation of IFN-alpha, IFN-beta, and IFN-epsilon Gene Expression in Human Cervical Epithelial Cells. Cell & Bioscience, 6 pgs. PMID: 29118969. (link)

  13. Lin, Gloria, et al. (2017). TTI-621 (SIRP-alpha-Fc), a CD47-Blocking Cancer Immunotherapeutic, Triggers Phagocytosis of Lymphoma Cells by Multiple Polarized Macrophage Subsets . PLOS One, 16 pgs. PMID: 29084248. (link)

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  15. Wang, Qiang, et al. (2017). The E3 Ubiquitin Ligase RNF185 Facilitates the cGAS-Mediated Innate Immune Response. PLOS Pathogens, 25 pgs. PMID: 28273161. (link)

  16. Kalkavan, Halime, et al. (2017). Spatiotemporally Restricted Arenavirus Replication Induces Immune Surveillance and Type I Interferon-Dependent Tumour Regression. Nature Communications, 14 pgs. PMID: 28248314. (link)

  17. Jonsson, K, et al. (2017). IFI16 is Required for DNA Sensing in Human Macrophages by Promoting Production and Function of cGAMP. Nature Communications, 17 pgs. PMID: 28186168. (link)

  18. Rahman, Masmudur, et al. (2017). Myxoma Virus dsRNA Binding Protein M029 Inhibits the Type I IFN-Induced Antiviral State in a Highly Species-Specific Fashion. Viruses, 22 pgs. PMID: 28157174. (link)

  19. Niu, Congrong, et al. (2017). The Smc5/6 Complex Restricts HBV When Localized to ND10 Without Inducing an Innate Immune Response and is Counteracted by the HBV X Protein Shortly After Infection. PLOS One, 32 pgs. PMID: 28095508. (link)

  20. Iyer, Shilpa, et al. (2017). Resistance to Type I Interferons is a Major Determinant of HIV-1 Transmission Fitness. PNAS, 10 pgs. PMID: 28069935. (link)

  21. Schwartz, Jordan (2017). Elucidation of the Plasmacytoid Dendritic Cell Response to HIV-1. University of Toronto, 190 pgs. PMID: no PMID. (link)

  22. Tasker, Carley, et al. (2016). IFN-epsilon Protects Primary Macrophages Against HIV Infection. JCI Insight, 17 pgs. PMID: 27942584. (link)

  23. Yamauchi, Shota, et al. (2016). STAT1 is Essential For the Inhibition of Hepatitis C Virus Replication by Interferon-lambda but Not by Interferon-alpha. Scientific Reports, 11 pgs. PMID: 27929099. (link)

  24. Pham, Tram, et al. (2016). Enhancing Virion Tethering by BST2 Sensitizes Productively and Latently HIV-Infected T Cells to ADCC Mediated by Broadly Neutralizing Antibodies. Scientific Reports, 15 pgs. PMID: 27853288. (link)

  25. Eschbaumer, Michael, et al. (2016). Systemic Immune Response and Virus Persistence After Foot-and-Mouth Disease Virus Infection of Naive Cattle and Cattle Vaccinated With a Homologous Adenovirus-Vectored Vaccine. BMC Veterinary Research, 18 pgs. PMID: 27634113. (link)

  26. Millrine, David, et al. (2016). Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production. PNAS, 6 pgs. PMID: 27601648. (link)

  27. Kmiec, Dorota, et al. (2016). Vpu-Mediated Counteraction of Tetherin is a Major Determinant of HIV-1 Interferon Resistance. mBio, 10 pgs. PMID: 27531907. (link)

  28. Boyd, David, et al. (2016). Adapting SHIVs In Vivo Selects for Envelope-Mediated Interferon-alpha Resistance. PLOS Pathogens, 19 pgs. PMID: 27399306. (link)

  29. Gomez, Alejandro M, et al. (2016). HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status. Journal of Immunology, 13 pgs. PMID: 27022194. (link)

  30. Gomez, Alejandro M, et al. (2015). HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes. Journal of Immunology, 10 pgs. PMID: 25637018. (link)

  31. Bis, Regina, et al. (2014). High Yield Soluble Bacterial Expression and Streamlined Purification of Recombinant Human Interferon Alpha-2a. Protein Expression and Purification, 9 pgs. PMID: 24794500. (link)

  32. Drewes, Julia, et al. (2014). Attenuation of Pathogenic Immune Responses During Infection with Human and Simian Immunodeficiency Virus (HIV/SIV) by the Tetracycline Derivative Minocycline. PLOS One, 15 pgs. PMID: 24732038. (link)

  33. Ding, Siyuan, et al. (2014). Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth. PLOS Biology, 14 pgs. PMID: 24409098. (link)

  34. Bolen, Christopher, et al. (2013). Dynamic Expression Profiling of Type I and Type III Interferon-Stimulated Hepatocytes Reveals a Stable Hierarchy of Gene Expression. Hepatology, 11 pgs. PMID: 23929627. (link)

  35. Mudhasani, Rajini, et al. (2013). IFITM-2 and IFITM-3 but Not IFITM-1 Restrict Rift Valley Fever Virus. JVI, 15 pgs. PMID: 23720721. (link)

  36. Griffiths, Stephen, et al. (2013). Age-Associated Increase of Low-Avidity Cytomegalovirus-Specifc CD8+ T Cells That Re-Express CD45RA. Journal of Immunology, 11 pgs. PMID: 23636061. (link)

  37. Wilson, Timothy R, et al. (2012). Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 12 pgs. PMID: 22763448. (link)

  38. Koning, Fransje, et al. (2009). Defining APOBEC3 Expression Patterns in Human Tissues and Hematopoietic Cell Subsets. JVI, 12 pgs. PMID: 19587057. (link)

  39. Dellgren, et al. (2009). Human Interferon-lambda3 is a Potent Member of the Type III Interferon Family. Genes and Immunity, 7 pgs. PMID: 18987645. (link)

  40. Davis, Ann, et al. (2008). A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells. Journal of Immunology, 11 pgs. PMID: 19050236. (link)

  41. Gerber, Scott A, et al. (2008). IFN-alpha induces transcription of hypoxia-inducible factor-1alpha to inhibit proliferation of human endothelial cells. Journal of Immunology, 26 pgs. PMID: 18606657. (link)

  42. Rempel, et al. (2008). Sialoadhesin Expressed on IFN-Induced Monocytes Binds HIV-1 and Enhances Infectivity. PLOS One, 9 pgs. PMID: 18414664. (link)

  43. Muhlbauer, et al. (2006). PD-L1 is induced in hepatocytes by viral infection and by interferon-alpha and -gamma and mediates T cell apoptosis. Journal of Hepatology. PMID: 16876901. (link)

  44. Francois, Catherine, et al. (2005). Quantification of Different Human Alpha Interferon Subtypes and Pegylated Interferon Activities by Measuring MxA Promoter Activation. Antimicrobial Agents and Chemotherapy, 6 pgs. PMID: 16127052. (link)

  45. Ramaswamy, et al. (2004). Specific Inhibition of Type I Interferon Signal Transduction by Respiratory Syncytial Virus. AJRCMB. PMID: 14722224. (link)

  46. Yang D, et al. (2020) Attenuated Interferon and Proinflammatory Response in SARS-CoV-2-Infected Human Dendritic Cells Is Associated With Viral Antagonism of STAT1 Phosphorylation. Journal of Infectious Diseases, 9 pgs. PMID: 32563187. (link)

  47. Oosenbrug T, et al. (2020) An alternative model for type I interferon induction downstream of human TLR2. Journal of Biological Chemistry, 26 pgs. PMID: 32796029. (link)

Documentation

Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
11100-1 CoA

11100-1 Certificate of Analysis (CoA)

11100 SDS

11100 Safety Data Sheet