Recombinant Human Interferon Alpha A (Alpha 2a) obtained from human leukocyte mRNA expressed in E.coli.

IFN-Alpha 2a is one of the many interferon alpha subtypes belonging to the Type I leukocyte IFN family. The Type I IFN family has antiviral, anti-proliferative, and natural killer cell activities.

Application Note

• A Comparison of the Binding of Interferon Alpha Subtypes to Isolated IFNAR1 and IFNAR2 with Activity in Antiviral and Antiproliferative Assays (link)

61 Citations

1. Carre, A. et al., (2025), "Interferon-α promotes HLA-B-restricted presentation of conventional and alternative antigens in human pancreatic β-cells", Nat Commun., 16(1):765, PMID: 39824805, DOI: 10.1038/s41467-025-55908-9 (link)
2. Leisching, G. et al., (2024), "Interferon α and β induce differential transcriptional and functional metabolic phenotypes in human macrophages and blunt glycolysis in response to antigenic stimuli", Eur. J. Immunol., e2451032, PMID: 38993003, DOI: 10.1002/eji.202451032 (link)
3. Baquero-Perez,, B. et al., (2024), "Elucidation of the Epitranscriptomic RNA Modification Landscape of Chikungunya Virus", Viruses, 16(6):945, DOI: 10.3390/v16060945 (link)
4. Lee, AM. et al., (2023), "Neutrophil-plasmacytoid dendritic cell interaction leads to production of type I IFN in response to Mycobacterium tuberculosis", Eur J Immunol., e2350666, PMID: 38161237, DOI: 10.1002/eji.202350666 (link)
5. Guo, H., et al., (2023), "Drug repurposing screen identifies vidofludimus calcium and pyrazofurin as novel chemical entities for the development of hepatitis E interventions", Virol Sin., S1995-820X(23)00138-4, PMID: 37984761, DOI: 10.1016/j.virs.2023.11.006 (link)
6. Lai, JH. et al., (2023), "USP18 enhances dengue virus replication by regulating mitochindrial DNA release", Sci Rep. 13(1):20126, PMID: 37978268, DOI: 10.1038/s41598-023-47584-w (link)
7. Wang, S. et al., (2023), "Deep mutational scanning of influenza A virus neuraminidase facilitates the identification of drug resistance mutations in vivo", mSystems, e0067023, PMID: 37772870, DOI: 10.1128/msystems.00670-23 (link)
8. Lodge, R. et al., (2023), "MicroRNA-25/93 induction by Vpu as a mechanism for counteracting MARCH1-restriction on HIV-1 infectivity in macrophages", mBio, e0195023, PMID: 37773002, DOI: 10.1128/mbio.01950-23 (link)
9. Crawford, J.D., et al., (2023), "The XIST lncRNA is a sex-specific reservoir of TLR7 ligands in SLE", JCI Insight., e169344, PMID: 37733447, DOI: 10.1172/jci.insight.169344 (link)
10. Cacace, R. et al., (2023), "Mutated Toll-like receptor 9 increases Alzheimer's disease risk by compromising innate immunity protection", Mol. Psychiatry, PIMID: 37433968, DOI: 10.1038/s41380-023-02166-0 (link)
11. Pecori, R. et al., (2023), "ADAR-1mediated RNA editing promotes B cell lymphomagenesis", iScience, DOI: 10.1016/j.isci.2023.106864 (link)
12. Wang, X. et al., (2023), "Susceptibility of Vero E6 cells to Tilapia Lake virus (TiLV) and anti-TiLV activity of type I iunterferon", Aquaculture, DOI: 10.1016/j.aquaculture.2023.739598 (link)
13. Laurent, P. et al., (2022), "Sensing of SARS-CoV-2 by pDCs and their subsequent production of IFN-I contribute to macrophage-induced cytokine storm during COVID-19", Sci Immunol., 7(75):eadd4906, PMID: 36083891, DOI: 10.1126/sciimmunol.add4906 (link)
14. Maier, B.D. et al., (2022), "Stochastic dynamics of Type-I interferon responses", PLoS Comput Biol., 18(10):e1010623, PMID: 36269758, DOI: 10.1371/journal.pcbi.1010623 (link)
15. Zhang, Q., et al.,(2022), "Autoantibodies against type I IFNs in patients with critical influenza pneumonia", J. Exp. Med. 219(11):e20220514, DOI: 10.1084/jem.20220514 (link)
16. Manokaran, G. et al., (2022), "Deactivation of the antiviral state by rabies virus through targeting and accumulation of persistently phosphorylated STAT1", PLoS Pathogens, 18(5):e1010533, PMID: 35576230, DOI: 10.1371/jpurnal.ppat.1010533 (link)
17. Alphonse, N. et al.,  (2022), "A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling", Cell, S0092-8674(22)00526-8, PMID: 35568036, DOI: 10.1016/j.cell.2022.04.028 (link)
18. Tomg, S., et al., (2022), "Inhibition of interferon-gamma-stimulated melanoma progression by targeting neuronal nitric oxide synthase (nNOS)', Scientific Reports, 12:1701, DOI: 10.1038/s41598-022-05394-6 (link)
19. Savvateeva, E. et al., (2021), "Microarray-Based Detection of Antibodies against SARS-CoV-2 Proteins, Common Respiratory Viruses and Type I Interferons", Viruses, 13:2553, DOI: 10.3390/v13122553 (link)
20. Yang, Dong, et al. (2020). Attenuated interferon and pro-inflammatory response in SARS-CoV-2-infected human dendritic cells is associated with viral antagonism of STAT1 phosphorylation. The Journal of Infectious Diseases, 34 pgs. PMID: 32563187. (link)
21. Kumar, Manickam, et al. (2020). In vitro replicative fitness of early Transmitted founder HIV-1 variants and sensitivity to Interferon alpha. Scientific Reports, 12 pgs. PMID: 32066770. (link)
22. Sharma, Amit, et al. (2019). Macaque interferon-induced transmembrane proteins limit replication of SHIV strains in an Envelope-dependent manner. PLOS Pathogens, 23 pgs. PMID: 31260493. (link)
23. Williams, Katherine, et al. (2019). Identification of HIV gp41-specific antibodies that mediate killing of infected cells. PLOS Pathogens, 27 pgs. PMID: 30779811. (link)
24. Perucha, Esperanza, et al. (2019). The cholesterol biosynthesis pathway regulates IL-10 expression in human Th1 cells. Nature Communications, 13 pgs. PMID: 30700717. (link)
25. DeMarino, Catherine, et al. (2018). Antiretroviral Drugs Alter the Content of Extracellular Vesicles from HIV-1 Infected Cells. Scientific Reports, 20 pgs. PMID: 29769566. (link)
26. Couret, Jennifer, et al. (2017). Differential Regulation of IFN-alpha, IFN-beta, and IFN-epsilon Gene Expression in Human Cervical Epithelial Cells. Cell & Bioscience, 6 pgs. PMID: 29118969. (link)
27. Lin, Gloria, et al. (2017). TTI-621 (SIRP-alpha-Fc), a CD47-Blocking Cancer Immunotherapeutic, Triggers Phagocytosis of Lymphoma Cells by Multiple Polarized Macrophage Subsets . PLOS One, 16 pgs. PMID: 29084248. (link)
28. von Stemann, Jakob H, et al. (2017). Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study. PLOS One, 17 pgs. PMID: 28665954. (link)
29. Wang, Qiang, et al. (2017). The E3 Ubiquitin Ligase RNF185 Facilitates the cGAS-Mediated Innate Immune Response. PLOS Pathogens, 25 pgs. PMID: 28273161. (link)
30. Kalkavan, Halime, et al. (2017). Spatiotemporally Restricted Arenavirus Replication Induces Immune Surveillance and Type I Interferon-Dependent Tumour Regression. Nature Communications, 14 pgs. PMID: 28248314. (link)
31. Jonsson, K, et al. (2017). IFI16 is Required for DNA Sensing in Human Macrophages by Promoting Production and Function of cGAMP. Nature Communications, 17 pgs. PMID: 28186168. (link)
32. Rahman, Masmudur, et al. (2017). Myxoma Virus dsRNA Binding Protein M029 Inhibits the Type I IFN-Induced Antiviral State in a Highly Species-Specific Fashion. Viruses, 22 pgs. PMID: 28157174. (link)
33. Niu, Congrong, et al. (2017). The Smc5/6 Complex Restricts HBV When Localized to ND10 Without Inducing an Innate Immune Response and is Counteracted by the HBV X Protein Shortly After Infection. PLOS One, 32 pgs. PMID: 28095508. (link)
34. Iyer, Shilpa, et al. (2017). Resistance to Type I Interferons is a Major Determinant of HIV-1 Transmission Fitness. PNAS, 10 pgs. PMID: 28069935. (link)
35. Schwartz, Jordan (2017). Elucidation of the Plasmacytoid Dendritic Cell Response to HIV-1. University of Toronto, 190 pgs. PMID: no PMID. (link)
36. Tasker, Carley, et al. (2016). IFN-epsilon Protects Primary Macrophages Against HIV Infection. JCI Insight, 17 pgs. PMID: 27942584. (link)
37. Yamauchi, Shota, et al. (2016). STAT1 is Essential For the Inhibition of Hepatitis C Virus Replication by Interferon-lambda but Not by Interferon-alpha. Scientific Reports, 11 pgs. PMID: 27929099. (link)
38. Pham, Tram, et al. (2016). Enhancing Virion Tethering by BST2 Sensitizes Productively and Latently HIV-Infected T Cells to ADCC Mediated by Broadly Neutralizing Antibodies. Scientific Reports, 15 pgs. PMID: 27853288. (link)
39. Eschbaumer, Michael, et al. (2016). Systemic Immune Response and Virus Persistence After Foot-and-Mouth Disease Virus Infection of Naive Cattle and Cattle Vaccinated With a Homologous Adenovirus-Vectored Vaccine. BMC Veterinary Research, 18 pgs. PMID: 27634113. (link)
40. Millrine, David, et al. (2016). Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production. PNAS, 6 pgs. PMID: 27601648. (link)
41. Kmiec, Dorota, et al. (2016). Vpu-Mediated Counteraction of Tetherin is a Major Determinant of HIV-1 Interferon Resistance. mBio, 10 pgs. PMID: 27531907. (link)
42. Boyd, David, et al. (2016). Adapting SHIVs In Vivo Selects for Envelope-Mediated Interferon-alpha Resistance. PLOS Pathogens, 19 pgs. PMID: 27399306. (link)
43. Gomez, Alejandro M, et al. (2016). HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status. Journal of Immunology, 13 pgs. PMID: 27022194. (link)
44. Gomez, Alejandro M, et al. (2015). HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes. Journal of Immunology, 10 pgs. PMID: 25637018. (link)
45. Bis, Regina, et al. (2014). High Yield Soluble Bacterial Expression and Streamlined Purification of Recombinant Human Interferon Alpha-2a. Protein Expression and Purification, 9 pgs. PMID: 24794500. (link)
46. Drewes, Julia, et al. (2014). Attenuation of Pathogenic Immune Responses During Infection with Human and Simian Immunodeficiency Virus (HIV/SIV) by the Tetracycline Derivative Minocycline. PLOS One, 15 pgs. PMID: 24732038. (link)
47. Ding, Siyuan, et al. (2014). Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth. PLOS Biology, 14 pgs. PMID: 24409098. (link)
48. Bolen, Christopher, et al. (2013). Dynamic Expression Profiling of Type I and Type III Interferon-Stimulated Hepatocytes Reveals a Stable Hierarchy of Gene Expression. Hepatology, 11 pgs. PMID: 23929627. (link)
49. Mudhasani, Rajini, et al. (2013). IFITM-2 and IFITM-3 but Not IFITM-1 Restrict Rift Valley Fever Virus. JVI, 15 pgs. PMID: 23720721. (link)
50. Griffiths, Stephen, et al. (2013). Age-Associated Increase of Low-Avidity Cytomegalovirus-Specifc CD8+ T Cells That Re-Express CD45RA. Journal of Immunology, 11 pgs. PMID: 23636061. (link)
51. Wilson, Timothy R, et al. (2012). Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 12 pgs. PMID: 22763448. (link)
52. Koning, Fransje, et al. (2009). Defining APOBEC3 Expression Patterns in Human Tissues and Hematopoietic Cell Subsets. JVI, 12 pgs. PMID: 19587057. (link)
53. Dellgren, et al. (2009). Human Interferon-lambda3 is a Potent Member of the Type III Interferon Family. Genes and Immunity, 7 pgs. PMID: 18987645. (link)
54. Davis, Ann, et al. (2008). A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells. Journal of Immunology, 11 pgs. PMID: 19050236. (link)
55. Gerber, Scott A, et al. (2008). IFN-alpha induces transcription of hypoxia-inducible factor-1alpha to inhibit proliferation of human endothelial cells. Journal of Immunology, 26 pgs. PMID: 18606657. (link)
56. Rempel, et al. (2008). Sialoadhesin Expressed on IFN-Induced Monocytes Binds HIV-1 and Enhances Infectivity. PLOS One, 9 pgs. PMID: 18414664. (link)
57. Muhlbauer, et al. (2006). PD-L1 is induced in hepatocytes by viral infection and by interferon-alpha and -gamma and mediates T cell apoptosis. Journal of Hepatology. PMID: 16876901. (link)
58. Francois, Catherine, et al. (2005). Quantification of Different Human Alpha Interferon Subtypes and Pegylated Interferon Activities by Measuring MxA Promoter Activation. Antimicrobial Agents and Chemotherapy, 6 pgs. PMID: 16127052. (link)
59. Ramaswamy, et al. (2004). Specific Inhibition of Type I Interferon Signal Transduction by Respiratory Syncytial Virus. AJRCMB. PMID: 14722224. (link)
60. Yang D, et al. (2020) Attenuated Interferon and Proinflammatory Response in SARS-CoV-2-Infected Human Dendritic Cells Is Associated With Viral Antagonism of STAT1 Phosphorylation. Journal of Infectious Diseases, 9 pgs. PMID: 32563187. (link)
61. Oosenbrug T, et al. (2020) An alternative model for type I interferon induction downstream of human TLR2. Journal of Biological Chemistry, 26 pgs. PMID: 32796029. (link)