Human IFN-Alpha Sampler Set
Catalog Number: 11002
Sampler set contains 12 distinct Human IFN-Alpha subtypes for differential activity studies.
$2,650.00
Product Info
The Human IFN-Alpha Sampler Set provides a collection of 12 distinct biologically active alpha interferon subtypes which enables studies of their activity in various applications.
- cDNAs of interferons were obtained from human leukocyte mRNA, and expressed in E.coli
- Provides 100,000 units of IFNa-A, IFNa-B2, IFNa-C, IFNa-D (1[Val114]), IFNa-F, IFNa-G, IFNa-H2, IFNa-I, IFNa-J1, IFNa-K, IFNa-4b, and IFNa-WA (corresponding to IFNa-2a, IFNa-8, IFNa-10, IFNa-1, IFNa-21, IFNa-5, IFNa-14, IFNa-17, IFNa-7, IFNa-6, IFNa-4b, IFNa-16, respectively)
Specifications
| Formulation | Individual interferons are supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
|---|---|
| Molecular Weight | 19.2 -19.7 kDa |
| Source | cDNA of interferons were obtained from human leukocyte mRNA, and expressed in E.coli |
| Purity | > 95% by SDS-PAGE stained by Coomassie Blue |
| Bioactivity | Activities of the interferons are measured using a cytopathic inhibition assay on Bovine Kidney Cells (MDBK) with Vesicular Stomatis Virus (VSV); human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV) |
| Storage | For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles |
| Synonyms | Interferon alpha subtypes, IFN alpha subtypes, IFN-a subtypes, interferon alpha, Leukocyte interferon subtypes, Leukocyte IFN subtypes, alpha interferon subtypes, interferon alfa subtypes, IFN alfa, interferon a, Type I interferon alpha, Type I IFN alpha, Human interferon alpha, IFN-alpha, INF alpha, Human IFN alpha 2a, human IFN alpha 8, human IFN alpha 10, human IFN alpha 1, human IFN alpha 21, human IFN alpha 5, human IFN alpha 14, human IFN alpha 17, human IFN alpha 7, human IFN alpha 6, human IFN alpha 4b, human IFN alpha 16 |
| Accession Number | Refer to CoA |
Tech Info & Data
Background
The Human interferon alpha protein family consists of 12 individual protein species, each with reported different biological properties. Some have proven to be 10 and 20 times more effective than Human IFN-alpha A in protecting a cell against specific viruses. Elucidating the functional significances of various IFN-alpha subtypes not only can lead to a better understanding of the role interferons have in an immunological event, but can also lead to the refinement of current interferon-based therapeutic applications.
| Human IFN Alpha Subtype Nomenclature | Gene | |
| IFN Alpha A | IFN Alpha 2a | IFNA2 |
| IFN Alpha 2 | IFN Alpha 2b | IFNA2 |
| IFN Alpha B2 | IFN Alpha 8 | IFNA8 |
| IFN Alpha C | IFN Alpha 10 | IFNA10 |
| IFN Alpha D [Val114] | IFN Alpha 1 | IFNA1 |
| IFN Alpha F | IFN Alpha 21 | IFNA21 |
| IFN Alpha G | IFN Alpha 5 | IFNA5 |
| IFN Alpha H2 | IFN Alpha 14 | IFNA14 |
| IFN Alpha I | IFN Alpha 17 | IFNA17 |
| IFN Alpha J1 | IFN Alpha 7 | IFNA7 |
| IFN Alpha K | IFN Alpha 6 | IFNA6 |
| IFN Alpha 1 [Ala114] | IFN Alpha D | IFNA1 |
| IFN Alpha 4a | IFN Alpha M1 | IFNA4 |
| IFN Alpha 4b | IFN Alpha 4 | IFNA4 |
| IFN Alpha WA | IFN Alpha 16 | IFNA16 |
For more information on Human IFN Alpha subtype molecular weight, and theoretical pI click here.
Citations
12 Citations
- Le Stang, V. et al., (2024), "Similar Kinetics of Pulmonary SARS-CoV-2 Load in Intensive Care Unit Patients with COVID-19 Pneumonia with or Without Autoantibodies Neutralizing Type I Interferons", J. Clin Immunol., 45(1):45, PMID: 39565497, DOI: 10.1007/s10875-024-01839 (link)
- Hannah, H. et al., (2023), Inactive disease in patients with lupus is linked to autoantibodies to typoe I interferons that normalize blood IFNa and B cell subsets, Cell Rep Med, 4(1):100894, PMID: 36652906, DOI:10.1016/j.xcrm.2022.100894 (link)
- Guo, K. et al., (2020), "Qualitative Differences Between the IFNa subtypes and IFNb Influenze Chronic Mucosal HIV-1 Pathogenesis", PLoS Pathog., 16(10):e1008986, PMID: 33064743, DOI: 10.1371/journal.ppat.1008986 (link)
- Prevost, Jeremie, et al. (2019). Upregulation of BST-2 by Type I Interferons Reduces the Capacity of Vpu To Protect HIV-1-Infected Cells from NK Cell Responses. mBio, 18 pgs. PMID: 31213558. (link)
- Schlaepfer, et al. (2019). Dose-Dependent Differences in HIV Inhibition by Different Interferon Alpha Subtypes While Having Overall Similar Biologic Effects. mSphere, 14 pgs. PMID: 30760614. (link)
- Cook, Bradley, et al. (2016). Limited Effects of Type I Interferons on Kyasanur Forest Disease Virus in Cell Culture. PLOS Neglected Tropical Diseases, 19 pgs. PMID: 27479197. (link)
- Lavender, Kerry, et al. (2016). Interferon Alpha Subtype-Specific Suppression of HIV-1 Infection in Vivo. JVI, 13 pgs. PMID: 27099312. (link)
- Todt, Daniel, et al. (2016). Antiviral Activities of Different Interferon Types and Subtypes Against Hepatitis E Virus Replication. Antimicrobial Agents and Chemotherapy, 8 pgs. PMID: 26787701. (link)
- Harper, Michael, et al. (2015). Interferon-alpha Subtypes in an Ex Vivo Model of Acute HIV-1 Infection: Expression, Potency, and Effector Mechanisms. PLOS Pathogens, 24 pgs. PMID: 26529416. (link)
- Schanen, C, et al. (2006). Correlation Between the Anti-Viral-Induced Cytopathic Effect of Interferon-alpha Subtypes and Induction of MxA Protein In Vitro. Microbiology and Immunology, 19 pgs. PMID: 16428869. (link)
- Francois, Catherine, et al. (2005). Quantification of Different Human Alpha Interferon Subtypes and Pegylated Interferon Activities by Measuring MxA Promoter Activation. Antimicrobial Agents and Chemotherapy, 6 pgs. PMID: 16127052. (link)
- Sperber, Steven, et al. (1992). Anti-HIV-1 activity of recombinant and hybrid species of interferon-alpha. Journal of Interferon Research, 6 pgs. PMID: 1331260. (link)
Background Literature:
For more information on the various alleles of IFN alpha 2a, 2b, 4a, 4b, 1 and D:
- Gewart, DR., et al. (1995). J Interferon Cytokine Res. 15 (5):403
- Hussain, M., et al. (1997). J Interferon Cytokine Res. 17 (9):559
- Hussain, M., et al. (2000). J Interferon Cytokine Res. 20:763