Cynomolgus IFN Beta ELISA Kit (Serum, Plasma, TCM)

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Product Features: 
  • Quantifies Cynomolgus IFN-Beta in serum, plasma, and tissue culture media (TCM) as low as 5.47 pg/ml
  • Reproducible results with inter- and intra-assay CVs ≤ 10%
  • Recombinant Mammalian Cynomolgus IFN Beta provided as the ELISA standard

Full Product Name: VeriKine Cynomolgus Interferon Beta ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
46415-1

1 x 96-well plate

 $740.00

Specifications

Compatibility: Serum, Plasma, Cell Culture Supernatant
Assay Range: 5.47 - 350 pg/ml
Speed: Incubation time, 3 hours
Specificity: Cynomolgus (Macaca fascicularis) Interferon Beta

 

Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Average Spike Recovery: 94%

 

Storage: 2-8°C
Expiration Date: 12 months from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Cynomolgus Interferon Beta Standard, 100,000 pg/ml
  • Standard Diluent
  • Sample Buffer 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Assay Diluent
  • TMB Substrate Solution
  • Stop Solution 

Tech Info / Data

Quick reference guide, procedure of PBL Cynomolgus IFN Beta ELISA (46415)
Background: 

Basal levels of Type I IFNs, including IFN-β, are not fully understood. They are believed to be important for robust response to pathogens and may play additional roles in cellular homeostasis. The VeriKine Cynomolgus IFN-beta ELISA has been developed to measure low/basal levels of non-human primate interferon beta (Macaca fascicularis IFN-β) in a variety of sample matrices including serum, plasma and tissue culture media (TCM) by sandwich enzyme-linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a biotinylated detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). The substrate is tetramethylbenzidine (TMB).

Citations

References: (For additional references, please refer to the protocol.)

  1. Krause et al. (2005). Pharma Ther. 106 (3):299-346.

  2. Weinstock-Guttman et al. (2008). Expert Opin Biol Ther. 8(9):1435-47.

  3. Lee et al. (2007). Mol Cells. 23(1):1-10. 

  4. Heim. (1999). J Recept Signal Transduct Res. 19(1-4):75-120.

  5. Taniguchi et al . (2002). Curr Opin Immunol . 14(1):111-6.

Documentation

Typical Cyno IFN Beta Standard Curve from 5.47 to 350 pg/ml using PBL's Cynomolgus IFN Beta ELISA (46415)

Cynomolgus IFN beta standard curve from 5.47 to 350 pg/ml using PBL Cynomolgus Interferon Beta ELISA (46415)

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