This ELISA quantifies Cynomolgus IFN-Beta in serum, plasma, and tissue culture media (TCM) with an LLOQ of 5.47 pg/ml. Recombinant mammalian Cynomolgus IFN-Beta is provided as the ELISA standard.
Product Name: VeriKine Cynomolgus Interferon-Beta ELISA Kit (Serum, Plasma, TCM)
Representative Cynomolgus IFN-Beta Standard Curve from 5.47 to 350 pg/ml using PBL's Cynomolgus IFN Beta ELISA (46415)
| Matrix Compatibility | Serum, Plasma, Cell Culture Supernatant |
|---|---|
| Assay Range | 5.47 - 350 pg/ml |
| LLOQ | 5.47 pg/ml Need more sensitivity? Check out our Sample Testing Services |
| Assay Length | 3 hours |
| Specificity | Cynomolgus (Macaca fascicularis) IFN-Beta |
The VeriKine Cynomolgus IFN-Beta ELISA has been developed to measure low/basal levels of non-human primate IFN-Beta (Macaca fascicularis IFN-Beta) in a variety of sample matrices including serum, plasma and tissue culture media (TCM) by sandwich enzyme-linked immunosorbent assay (ELISA).
Certificate of Analysis (CoA), Protocol, and Safety Data Sheet (SDS)
46415-1 Certificate of Analysis (CoA) & Protocol (One-Page)
46415 Protocol (Full)
46415-1 Certificate of Analysis (CoA)
46415 Product Flyer
46415 Safety Data Sheet
| CVs and Spike Recovery | Inter-Assay ≤ 10%
Average Spike Recovery: 94% |
|---|---|
| Storage | 2-8°C |
| Expiration Date | One year from the date of manufacture |
| Shipping Condition | Wet Ice |
Intra and Inter-Assay CVs to measure Precision | |||
|---|---|---|---|
| L | M | H | |
| Concentration (pg/ml) | 6 | 40 | 200 |
| Intra-Assay CV (%) | 2.5 | 1.6 | 2.8 |
Inter-Assay CV (%) Operator 1 (n=4) | 5.4 | 4.3 | 2.3 |
Inter-Assay CV (%) Operator 2 (n=3) | 4.4 | 3.4 | 5.8 |
| Average % Recovery | 93.7 | 97.1 | 92.4 |
Interferons (IFNs) are a group of cytokines that exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of multiple IFN Alpha (α) genes and at least one IFN-Beta (β) gene in most vertebrates. IFN-β is used therapeutically to treat Multiple Sclerosis. Although well studied in humans and mice, the role of IFN-Beta in Rhesus and Cynomolgus monkey responses is relatively limited.
IFN-β expression and secretion are primarily induced by signaling from pattern recognition receptors such as Toll-like (TLR) and RIG-I-like receptors (RLR). Overall, IFN-β is part of the first wave of cytokine response in cells. Pathogen infection can result in the activation of interferon regulatory factor 3 (IRF3) which functions in trans to activate IFN-β gene transcription.
Following expression and secretion, IFN-β binds to a transmembrane heterodimeric receptor chain consisting of IFNAR1 & IFNAR2 on infected (autocrine) or neighboring cell (paracrine) surfaces. Receptor binding promotes a signal transduction cascade consisting of components of the JAK-STAT signaling pathway. This results in the expression of many genes including interferon regulatory factor 7 (IRF7) which upregulates the expression of many IFN-α subtype proteins. The IRF3/IRF7 signaling cascade is important for the initial and progressive responses to pathogens wherein hundreds of genes are regulated in a coordinated, temporal manner.
IFN-β is biologically unique when compared to other interferons. Studies have shown that IFN-β has overlapping and distinct gene expression patterns as compared to IFN-α. It appears that IFN-β binds to the Type 1 IFN receptor with higher affinity than other Type I IFNs and that it may also regulate receptor internalization in a different manner.
Basal levels of Type I IFNs, including IFN-β, are not fully understood. They are believed to be important for a robust response to pathogens and may play additional roles in cellular homeostasis.
This immunoassay has been developed to measure low levels of non-human primate IFN-Beta in a variety of sample matrices by sandwich enzyme-linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a biotinylated detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). The substrate is tetramethylbenzidine (TMB).
Powered by Bioz