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Human IFN-Alpha Hybrid Protein (Universal Type I IFN)

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Human IFN-Alpha Hybrid Protein (Universal Type I IFN)

Catalog Number: 11200

Well-cited recombinant Human IFN-Alpha protein hybrid exhibiting activity across multiple species. Useful for in vivo or in vitro studies where the species-relevant IFN-Alpha is unavailable or performs inconsistently.

$480.00

Pack Size
Product Info

Universal Type I IFN-Alpha is a unique hybrid interferon constructed from recombinant human interferons alpha A and alpha D (Human IFN-Alpha A/D [BglII]). Activity has been observed on many mammalian cells, including human, monkey, mouse, bovine, rat, cat, pig, rabbit, guinea pig, or hamster.

 

This product is useful for cross-species testing with interferon or for species where autologous interferon is not available. Crucial for cross-species comparative studies in virology, immune modulation, and more.

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.4 kDa
Source Construct described in Rehberg, E., (1982) J. Biol. Chem. 257: 11497 derived from E.coli
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic inhibition assay on Bovine Kidney Cells (MDBK) with Vesicular Stomatis Virus (VSV); human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV); Mouse (L929) cells with EMCV
Storage For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles
Synonyms Hu-IFN-αA/D[Bg/II], Type I Interferon Alpha Hybrid, Universal Type I Interferon Alpha, Type I IFN Alpha, Cross Species IFN Alpha, A/D-BgI II, Type I Interferon Protein, Type I IFN Alpha
Accession Number N/A

Citations

74 Citations:

 

  1. Kedarinath, K. et al., (2022), "CD24 Expression Sampens the Basal Antiviral State in Human Neuroblastoma Cells and Enhances Permissivity to Zika Virus Infection", Viruses, 14:1735, DOI: 10.3390/v14081735 (link)

  2. Sato, Y. et al., (2022), "Epstein-Barr virus tegument protein BGLF2 in exosomes released from virus-producing cells facilitates de novo infection", Cell Commun. SIgnal., 20(1):95, PMID: 35729616, DOI: 10.1186/s12964-022-00902-7 (link)

  3. Lutz, K. et al., (2022), "Ly6D+Siglec-H+ precursors contribute to conventional dendritic cells via a Zbtb46+Ly6D+ intermediary stage", Nat. Commun., 13(1):3456, PMID: 35705536, DOI: 10.1038/s41467-022-31054-4 (link)

  4. Manokaran, G. et al., (2022), "Deactivation of the antiviral state by rabies virus through targeting and accumulation of persistently phosphorylated STAT1", PLoS Pathogens, 18(5):e1010533, PMID: 35576230, DOI: 10.1371/jpurnal.ppat.1010533 (link)

  5. Kedarinath, K. and Parks, G. D., (2022), "Differential In Vitro Growth and Cell Killing of Cancer versus Benign Prostate Cells by Oncolytic Parainfluenza Virus", Pathogens, 11(5):493, DOI: 10.3390/pathogens11050493 (link)

  6. Gnatenko, D., et al., (2022), "Cytokine pathway variants modulate platelet production: IFNA16 is a thrombocytosis susceptibility locus in humans", Blood Advances, PMID: 35381074, DOI: 10.1182/bloodadvances.2021005648 (link)

  7. Cheng, CW. et al., (2022), "The pathogenic role of IFN-a in thyroiditis mouse model", Life Sciences, 288:120172, DOI: 10.1016/j.lfs.2021.120172 (link)

  8. Ka, N., et al.,  (2021), "IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer", Cell Reports, 37:110138, DOI: 10.1016/j.celrep.2021.110138 (link)

  9. Amat, J.A.R. et al., (2021), "Long-term adaptation following influenza A virus host shifts results in increased within-host viral fitness due to higher replication rates, broader dissemination within the respiratory epthelium and reduced tissue damage", PLoS Pathog., 17(12):e1010174, DOI: 10.1371/journal.ppat.1010174 (link)

  10. Brunn, D. et al.,  (2021), "Interferon Regulatory Factor 9 Promotes Lung Cancer Progression via Regulation of Versican", Cancers, 13:208, PMID: 33430083, DOI: 10.3390/cancers13020208 (link)

  11. Liu W, et al. (2021) Activation of STING signaling pathway effectively blocks human 1 coronavirus infection. American Society for Microbiology (link)

  12. Rebendenne A, et al. (2021) SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to 1control replication in lung epithelial cells. American Society for Microbiology, PMID: 33514628 (link)

  13. Cheng, J., et al., (2021), "IL-27 induces IFN/STAT1-dependent genes and enhances function of TIGIT+ HIVGag-specific T cells", iScience, 25:103588, DOI: 10.1016/j.isci.2021.103588. (link)

  14. Shu, J. et al., (2021), "NS5-Independent Ablation of STAT2 by Zika virus to antagonize interferon signalling", Emerging Microbes & Infections, 10:1601, DOI: 10.1080/22221751.2021.1964384 (link)

  15. Zhan, J., et al., (2021), "Definition of the immune evasion-replication interface of rabies virus P protein", PLoS Patho, 17(7):e1009729, DOI: 10.1371/journal.ppat.1009129 (link)

  16. Hsu, JJC., et al., (2021), "Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein", PNAS, 118(24)e2101161118, DOI: 10.1073/pnas.2101161118

  17. Felgenhauer U, et al. (2020) Inhibition of SARS-CoV-2 by Type I and Type III Interferons. Journal of Biological Chemistry, PMID: 32587093. (link)

  18. Roesch F and OhAinle M. (2020) HIV-CRISPR: A CRISPR/Cas9 Screening Method to Identify Genes Affecting HIV Replication. Bio-Protocol (link)

  19. Satterfield, Benjamin, et al. (2019). Antagonism of STAT1 by Nipah virus P gene products modulates disease course but not lethal outcome in the ferret model. Scientific Reports, 18 pgs. PMID: 31723221. (link)

  20. Kim, Jeong Jin, et al. (2019). Viperin Differentially Induces Interferon-Stimulated Genes in Distinct Cell Types. Immune Network, 16 pgs. PMID: 31720044. (link)

  21. Heilmann, Emmanuel, et al. (2019). The Methyltransferase Region of Vesicular Stomatitis Virus L Polymerase Is a Target Site for Functional Intramolecular Insertion. Viruses, 18 pgs. PMID: 31717818. (link)

  22. Wang, Peng, et al. (2019). IL-36 promotes anti-viral immunity by boosting sensitivity to IFN-α/β in IRF1 dependent and independent manners. Nature Communications, 17 pgs. PMID: 31619669. (link)

  23. Vannini, Andrea, et al. (2019). αvβ3-integrin regulates PD-L1 expression and is involved in cancer immune evasion. PNAS, 11 pgs. PMID: 31527243. (link)

  24. Chen, Jianzhou, et al. (2019). Type I IFN protects cancer cells from CD8+ T cell–mediated cytotoxicity after radiation. JCI, 16 pgs. PMID: 31483286. (link)

  25. Robison, Amanda, et al. (2019). Expression of human TLR4/myeloid differentiation factor 2 directs an early innate immune response associated with modest increases in bacterial burden during Coxiella burnetii infection. Innate Immunity, 11 pgs. PMID: 31180798. (link)

  26. Grunewald, Matthew, et al. (2019). The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression. PLOS Pathogens, 24 pgs. PMID: 31095648. (link)

  27. Rigby, Rachel, et al. (2019). PA-X antagonises MAVS-dependent accumulation of early type I interferon messenger RNAs during influenza A virus infection. Scientific Reports, 13 pgs. PMID: 31076606. (link)

  28. Hossain, et al. (2019). Structural Elucidation of Viral Antagonism of Innate Immunity at the STAT1 Interface. Cell Reports, 21 pgs. PMID: no PMID. (link)

  29. Uccellini, Melissa, et al. (2018). ISRE-Reporter Mouse Reveals High Basal and Induced Type I IFN Responses in Inflammatory Monocytes. Cell Reports, 17 pgs. PMID: 30517866. (link)

  30. Pfaller, Christian, et al. (2018). Extensive Editing of Cellular and Viral Double-Stranded RNA Structures Accounts for Innate Immunity Suppression and the Proviral Activity of ADAR1^(p150). PLOS Biology, 36 pgs. PMID: 30496178. (link)

  31. Gkirtzimanaki, Katerina, et al. (2018). IFN-Alpha Impairs Autophagic Degradation of mtDNA Promoting Autoreactivity of SLE Monocytes in a STING-Dependent Fashion. Cell Reports, 19 pgs. PMID: 30355498. (link)

  32. Muth, Doreen, et al. (2018). Attenuation of Replication by a 29 Nucleotide Deletion in SARS-Coronavirus Acquired During the Early Stages of Human-to-Human Transmission. Scientific Reports, 11 pgs. PMID: 30310104. (link)

  33. Kruger, Nadine, et al. (2018). Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus. Cell Reports, 17 pgs. PMID: 30304672. (link)

  34. Carlin, Aaron, et al. (2018). Deconvolution of Pro- and Antiviral Genomic Responses in Zika Virus-Infected and Bystander Macrophages. PNAS, 10 pgs. PMID: 30206152. (link)

  35. Vahedi, et al. (2018). IL-15 and IFN-γ signal through the ERK pathway to inhibit HCV replication, independent of type I IFN signaling. Cytokine. PMID: 29908921. (link)

  36. Matsuno, Keita, et al. (2018). The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Phlebovirus. mSphere, 14 pgs. PMID: 29898985. (link)

  37. Cytlak, Urszula, et al. (2018). Ikaros family zinc finger 1 regulates dendritic cell development and function in humans. Nature Communications, 10 pgs. PMID: 29588478. (link)

  38. Rose, Elaine (2018). Identifying Mechanisms of Resistance to Oncolytic Virotherapy in Acute Leukemia Through a Genome-Wide CRISPR Screen. University of Ottawa, 108 pgs. PMID: no PMID. (no link)

  39. Dai, Xiuju, et al. (2017). Epidermal keratinocytes sense dsRNA via the NLRP3 inflammasome, mediating interleukin (IL)-1β and IL-18 release. Experimental Dermatology. PMID: 28266737. (link)

  40. Chen, et al. (2016). Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer. Nature, 34 pgs. PMID: 27225120. (link)

  41. Hedges, Jodi, et al. (2016). Type I Interferon Counters or Promotes Coxiella burnetii Replication Dependent on Tissue. Infection and Immunity, 11 pgs. PMID: 27068091. (link)

  42. Labzin, Larisa I, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)

  43. Berri, Fatma, et al. (2014). Annexin V incorporated into influenza virus particles inhibits gamma interferon signaling and promotes viral replication. JVI, 14 pgs. PMID: 25031344. (link)

  44. Cimica, Velasco, et al. (2014). An innate immunity-regulating virulence determinant is uniquely encoded by the Andes virus nucleocapsid protein. mBio, 10 pgs. PMID: 24549848. (link)

  45. Li, et al. (2014). RIG-I modulates Src-mediated AKT activation to restrain leukemic stemness. CellPress. PMID: 24412064. (link)

  46. Wang, Tao, et al. (2014). SECTM1 produced by tumor cells attracts human monocytes via CD7-mediated activation of the PI3K pathway. JID, 22 pgs. PMID: 24157461. (link)

  47. Li, Wen, et al. (2013). Regulation of development of CD56 bright CD11c + NK-like cells with helper function by IL-18. PLOS One, 12 pgs. PMID: 24376549. (link)

  48. Webster, Brian, et al. (2013). Evasion of superinfection exclusion and elimination of primary viral RNA by an adapted strain of hepatitis C virus. JVI, 16 pgs. PMID: 24089557. (link)

  49. Jones, Philip H, et al. (2013). BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1). Virology, 28 pgs. PMID: 23411007. (link)

  50. Srivastava, Raghvendra M, et al. (2013). Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients. Clinical Cancer Research, 26 pgs. PMID: 23444227. (link)

  51. Dionne, et al. (2013). Daxx Upregulation within the Cytoplasm of Reovirus-Infected Cells is Mediated by Interferon and Contributes to Apoptosis. JVI, 14 pgs. PMID: 23302889. (link)

  52. Lochhead, et al. (2012). Endothelial Cells and Fibroblasts Amplify the Arthritogenic Type I IFN Response in Murine Lyme Disease and are Major Sources of Chemokines in Borrelia burgdorferi-Infected Joint Tissue. Journal of Immunology, 34 pgs. PMID: 22851707. (link)

  53. Wilson, Timothy R, et al. (2012). Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 12 pgs. PMID: 22763448. (link)

  54. Curtsinger, et al. (2012). Autocrine IFN-gamma Promotes Naive CD8 T Cell Differentiation and Synergizes with IFN-alpha to Stimulate Strong Function. Journal of Immunology, 21 pgs. PMID: 22706089. (link)

  55. Marozin, et al. (2012). Posttranslational Modification of Vesicular Stomatitis Virus Glycoprotein, but Not JNK Inhibition, is the Antiviral Mechanism of SP600125. JVI, 12 pgs. PMID: 22345438. (link)

  56. Cilloniz, Cristian, et al. (2012). Molecular signatures associated with Mx1-mediated resistance to highly pathogenic influenza virus infection: mechanisms of survival. JVI, 10 pgs. PMID: 22190720. (link)

  57. Varga, et al. (2011). The Influenza Virus Protein Pb1-F2 Inhibits the Induction of Type 1 Interferon at the Level of the MAVS Adaptor Protein. PLOS Pathogens, 16 pgs. PMID: 21695240. (link)

  58. Knoblach, Theresa, et al. (2011). Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-γ. PLOS Pathogens, 24 pgs. PMID: 21533215. (link)

  59. Meunier and von Messling, et al. (2011). NS1-Mediated Delay of Type 1 Interferon Induction Contributes to Influenza A Virulence in Ferrets. Journal of General Virology, 10 pgs. PMID: 21411677. (link)

  60. Carlos, TS, et al. (2009). Parainfluenza virus 5 genomes are located in viral cytoplasmic bodies whilst the virus dismantles the interferon-induced antiviral state of cells. Journal of General Virology, 10 pgs. PMID: 19458173. (link)

  61. Ahmed, Maryam, et al. (2009). Vesicular Stomatitis Virus M Protein Mutant Stimulates Maturation of Toll-Like Receptor 7 (TLR7)-Positive Dendritic Cells through TLR-Dependent and -Independent Mechanisms. JVI, 14 pgs. PMID: 19144711. (link)

  62. Albanesi, Cristina, et al. (2009). Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment. JEM, 10 pgs. PMID: 19114666. (link)

  63. Carthagena, et al. (2008). Implications of TRIMalpha and TRIMcyp in Interferon-Induced Anti-Retroviral Restriction Activities. Retrovirology, 15 pgs. PMID: 18613956. (link)

  64. Han, Jian-Qiu, et al. (2007). A Phylogenetically Conserved RNA Structure in the Poliovirus Open Reading Frame Inhibits the Antiviral Endoribonuclease RNase L. JVI, 12 pgs. PMID: 17344297. (link)

  65. Ye, Ye, et al. (2007). Mouse Hepatitis Coronavirus A59 Nucleocapsid Protein Is a Type I Interferon Antagonist. JVI, 10 pgs. PMID: 17182678. (link)

  66. Pejawar, Sharmila, et al. (2005). Abortive versus Productive Viral Infection of Dendritic Cells with a Paramyxovirus Results in Differential Upregulation of Select Costimulatory Molecules. JVI, 14 pgs. PMID: 15919909. (link)

  67. Curtsinger, Julie, et al. (2005). Type I IFNs provide a third signal to CD8 T cells to stimulate clonal expansion and differentiation. Journal of Immunology, 6 pgs. PMID: 15814665. (link)

  68. Kambayashi, Taku, et al. (2003). Memory CD8+ T cells provide an early source of IFN-gamma. Journal of Immunology, 11 pgs. PMID: 12594263. (link)

  69. Sainz, Bruno, et al. (2002). Alpha/Beta Interferon and Gamma Interferon Synergize To Inhibit the Replication of Herpes Simplex Virus Type 1. JVI, 10 pgs. PMID: 12388715. (link)

  70. Weber, Friedemann, et al. (2002). Bunyamwera Bunyavirus Nonstructural Protein NSs Counteracts the Induction of Alpha/Beta Interferon. JVI, 7 pgs. PMID: 12133999. (link)

  71. McNally, James, et al. (2001). Attrition of Bystander CD8 T Cells during Virus-Induced T-Cell and Interferon Responses. JVI, 12 pgs. PMID: 11390598. (link)

  72. Doughty, Lesley, et al. (2001). A role for IFN-alpha beta in virus infection-induced sensitization to endotoxin. Journal of Immunology, 8 pgs. PMID: 11160329. (link)

  73. Abe, Shuzo, et al. (1999). Effects of chronic administration of interferon alpha A/D on serotonergic receptors in rat brain. Neurochemical Research, 5 pgs. PMID: 10215509. (link)

  74. Sperber, Steven, et al. (1992). Anti-HIV-1 activity of recombinant and hybrid species of interferon-alpha. Journal of Interferon Research, 6 pgs. PMID: 1331260. (link)

Documentation

Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
11200-1 CoA

11200-1 Certificate of Analysis (CoA)

11200-2 CoA

11200-2 Certificate of Analysis (CoA)

11200 SDS

11200 Safety Data Sheet