Mouse IFN Beta, carrier-free

Product Features: 
  • Recombinant Mouse IFN Beta without carrier protein
  • Less than 1 EU/μg endotoxin
  • Suitable for in vivo injection studies

Ordering

Catalog No. Pack Size Price Quantity
12401-1

1 x 105 units

$380.00

Specifications

Formulation Supplied frozen in 20 mM HEPES, pH 6.0, 0.5M NaCl; 6% Glycerol
Molecular Weight 19.6 kDa
Source Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" ( J. Interferon Res. 12: 139-43)
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV. EC50 for IFNβ is ~ 2.5 U/ml
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms Mouse Beta IFN, Mouse Beta Interferon, Mouse IFN Beta, Mouse IFNB, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta

Tech Info / Data

Background: 

E.coli expressed recombinant Mouse IFN Beta protein supplied in carrier-free formulation to minimize possible immnogenic reaction by host animal in in vivo studies

Citations

Comparison of Mouse IFN Beta with carrier and Mouse IFN Beta carrier-free

12401-1 Mouse IFN Beta Carrier-free

The activity of Mouse IFN Beta, carrier-free and Mouse IFN Beta with carrier was compared in the L929/EMCV CPE assay. The EC 50 for Mouse IFN Beta, carrier-free was 1.5 U/ml when calibrated to the international standard.

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