Mouse IFN-Beta, Carrier-Free
Catalog Number: 12401
E.coli-expressed recombinant Mouse IFN-Beta protein supplied in carrier-free formulation to minimize possible immunogenic reaction by host animal in in vivo studies.
$425.00
Product Info
Application
Bioactivity
Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV. EC50 for IFNβ is ~ 2.5 U/ml
Specifications
| Formulation | Supplied frozen in 20 mM HEPES, pH 6.0, 0.5M NaCl; 6% Glycerol |
|---|---|
| Molecular Weight | 19.6 kDa |
| Source | Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992) "Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" ( J. Interferon Res. 12: 139-43) |
| Purity | > 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
| Storage | For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles |
| Synonyms | Mouse Beta IFN, Mouse Beta Interferon, Mouse IFN Beta, Mouse IFNB, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta |
Citations
24 Citations
- Lee, K, M-C., et al., (2022). "Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization", Cell Reports, 39(3):110719, PMID: 35443173, DOI: 10.1016/j.celrep.2022.110719 (link)
- Yoon, S. et al., (2022), "Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes", Cell Death & Differentiation, DOI: 10.1038/s41418-021-00924-7 (link)
- Grunewald, Matthew, et al. (2019). The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression. PLOS Pathogens, 24 pgs. PMID: 31095648. (link)
- Wang, A, et al. (2019). Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice. PNAS. PMID: 30674681. (link)
- Li, S, et al. (2018). The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING. Cell Reports, 25 pgs. PMID: 30566866. (link)
- Cui, Huachun, et al. (2018). Interferon regulatory factor 2 inhibits expression of glycolytic genes and lipopolysaccharide induced pro-inflammatory responses in macrophages. Journal of Immunology. PMID: 29563175. (link)
- McDonough, Ashley, et al. (2017). Ischemia/Reperfusion Induces Interferon-Stimulated Gene Expression in Microglia. The Journal of Neuroscience, 17 pgs. PMID: 28747383. (link)
- Stier, Matthew, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology. PMID: 28576981. (link)
- Benci, Joseph, et al. (2016). Tumor Interferon Signaling Regulates a Multigenic Resistance Program to Immune Checkpoint Blockade. CellPress, 28 pgs. PMID: 27912061. (link)
- Labzin,Larisa, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)
- Majumdar, Tanmay, et al. (2015). Induction of Interferon-Stimulated Genes by IRF3 Promotes Replication of Toxoplasma gondii. PLOS Pathogens, 22 pgs. PMID: 25811886. (link)
- Welsby, Iain, et al. (2014). PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation*. JBC, 16 pgs. PMID: 25086041. (link)
- Duong, Sean, et al. (2014). Polymicrobial sepsis alters Ag-dependent and -independent memory CD8 T cell functions. Journal of Immunology. PMID: 24646738. (link)
- Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)
- Munitic, Ivana, et al. (2013). Optineurin Insufficiency Impairs Interferon Regulatory Factor 3 (IRF3) but not NF-κB Activation in Immune Cells. Journal of Immunology. PMID: 24244017. (link)
- Freeman, Bailey, et al. (2012). Regulation of innate CD8+ T-cell activation mediated by cytokines. PNAS, 6 pgs. PMID: 22665806. (link)
- Sutherland, DB, et al. (2011). Evaluating vaccinia virus cytokine co-expression in TLR GKO mice. Immunology and Cell Biology. PMID: 21173782. (link)
- Leu, Shaw-Wei, et al. (2011). TLR4 through IFN-β promotes low molecular mass hyaluronan-induced neutrophil apoptosis. Journal of Immunology, 8 pgs. PMID: 21098223. (link)
- Yamada, Atashi, et al. (2009). IFN-γ down-regulates Secretoglobin 3A1 gene expression. Biochemical and Biophysical Research Communications. PMID: 19135978. (link)
- Guo, Beichu, et al. (2008). The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. JCI, 11 pgs. PMID: 18382764. (link)
- Molle, Celine, et al. (2007). IL-27 Synthesis Induced by TLR Ligation Critically Depends on IFN Regulatory Factor 3. Journal of Immunology, 10 pgs. PMID: 17548596. (link)
- Jaini, et al. (2006). Gene-Based Intramuscular Interferon-beta Therapy for Experimental Autoimmune Encephalomyelitis. Molecular Therapy, 7 pgs. PMID: 16782409. (link)
- Braun, Benjamin, et al. (2006). Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood Journal. PMID: 16720837. (link)
- Zhang, F, et al. (2006). Differential growth of IFN-beta-engineered tumor cells in nude and IFN receptor-null mice. Journal of Interferon & Cytokine Research. PMID: 16487031. (link)
References
- Mesplede et al. (2003) Autoimmunity 36(8):447.
- Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.
- Higashi et al. (1983) J. Biol. Chem. 258(15):9522.
- Hayashi et al. (2002) J. Immunol. 277(31):27880.
- Fujimura et al. (2006) Infect. Immu. 75(5):2544.
- Day, et al. (1992) J. Interferon Res. 12: 139-43.
- Jaini et al. (2006) Mol. Ther. 14(3):416.
- Zhou and Perleman (2007) J. Vir. 81(2):568.
- Kamath et al. (2005) J. Immunol. 174(2):767.