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Mouse IFN-Beta, Carrier-Free

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Mouse IFN-Beta, Carrier-Free

Catalog Number: 12401

E.coli-expressed recombinant Mouse IFN-Beta protein supplied in carrier-free formulation to minimize possible immunogenic reaction by host animal in in vivo studies.

$425.00

Pack Size
Product Info

Application
  • in vivo injection studies
Bioactivity Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV. EC50 for IFNβ is ~ 2.5 U/ml

Specifications

Formulation Supplied frozen in 20 mM HEPES, pH 6.0, 0.5M NaCl; 6% Glycerol
Molecular Weight 19.6 kDa
Source Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" ( J. Interferon Res. 12: 139-43)
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms Mouse Beta IFN, Mouse Beta Interferon, Mouse IFN Beta, Mouse IFNB, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta

Citations

24 Citations

 

  1. Lee, K, M-C., et al., (2022). "Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization", Cell Reports, 39(3):110719, PMID: 35443173, DOI: 10.1016/j.celrep.2022.110719 (link)

  2. Yoon, S. et al., (2022), "Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes", Cell Death & Differentiation, DOI: 10.1038/s41418-021-00924-7 (link)

  3. Grunewald, Matthew, et al. (2019). The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression. PLOS Pathogens, 24 pgs. PMID: 31095648. (link)

  4. Wang, A, et al. (2019). Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice. PNAS. PMID: 30674681. (link)

  5. Li, S, et al. (2018). The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING. Cell Reports, 25 pgs. PMID: 30566866. (link)

  6. Cui, Huachun, et al. (2018). Interferon regulatory factor 2 inhibits expression of glycolytic genes and lipopolysaccharide induced pro-inflammatory responses in macrophages. Journal of Immunology. PMID: 29563175. (link)

  7. McDonough, Ashley, et al. (2017). Ischemia/Reperfusion Induces Interferon-Stimulated Gene Expression in Microglia. The Journal of Neuroscience, 17 pgs. PMID: 28747383. (link)

  8. Stier, Matthew, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology. PMID: 28576981. (link)

  9. Benci, Joseph, et al. (2016). Tumor Interferon Signaling Regulates a Multigenic Resistance Program to Immune Checkpoint Blockade. CellPress, 28 pgs. PMID: 27912061. (link)

  10. Labzin,Larisa, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)

  11. Majumdar, Tanmay, et al. (2015). Induction of Interferon-Stimulated Genes by IRF3 Promotes Replication of Toxoplasma gondii. PLOS Pathogens, 22 pgs. PMID: 25811886. (link)

  12. Welsby, Iain, et al. (2014). PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation*. JBC, 16 pgs. PMID: 25086041. (link)

  13. Duong, Sean, et al. (2014). Polymicrobial sepsis alters Ag-dependent and -independent memory CD8 T cell functions. Journal of Immunology. PMID: 24646738. (link)

  14. Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)

  15. Munitic, Ivana, et al. (2013). Optineurin Insufficiency Impairs Interferon Regulatory Factor 3 (IRF3) but not NF-κB Activation in Immune Cells. Journal of Immunology. PMID: 24244017. (link)

  16. Freeman, Bailey, et al. (2012). Regulation of innate CD8+ T-cell activation mediated by cytokines. PNAS, 6 pgs. PMID: 22665806. (link)

  17. Sutherland, DB, et al. (2011). Evaluating vaccinia virus cytokine co-expression in TLR GKO mice. Immunology and Cell Biology. PMID: 21173782. (link)

  18. Leu, Shaw-Wei, et al. (2011). TLR4 through IFN-β promotes low molecular mass hyaluronan-induced neutrophil apoptosis. Journal of Immunology, 8 pgs. PMID: 21098223. (link)

  19. Yamada, Atashi, et al. (2009). IFN-γ down-regulates Secretoglobin 3A1 gene expression. Biochemical and Biophysical Research Communications. PMID: 19135978. (link)

  20. Guo, Beichu, et al. (2008). The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. JCI, 11 pgs. PMID: 18382764. (link)

  21. Molle, Celine, et al. (2007). IL-27 Synthesis Induced by TLR Ligation Critically Depends on IFN Regulatory Factor 3. Journal of Immunology, 10 pgs. PMID: 17548596. (link)

  22. Jaini, et al. (2006). Gene-Based Intramuscular Interferon-beta Therapy for Experimental Autoimmune Encephalomyelitis. Molecular Therapy, 7 pgs. PMID: 16782409. (link)

  23. Braun, Benjamin, et al. (2006). Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood Journal. PMID: 16720837. (link)

  24. Zhang, F, et al. (2006). Differential growth of IFN-beta-engineered tumor cells in nude and IFN receptor-null mice. Journal of Interferon & Cytokine Research. PMID: 16487031. (link)

     

    References

     

    1. Mesplede et al. (2003) Autoimmunity 36(8):447.

    2. Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

    3. Higashi et al. (1983) J. Biol. Chem. 258(15):9522.

    4. Hayashi et al. (2002) J. Immunol. 277(31):27880.

    5. Fujimura et al. (2006) Infect. Immu. 75(5):2544.

    6. Day, et al. (1992) J. Interferon Res. 12: 139-43.

    7. Jaini et al. (2006) Mol. Ther. 14(3):416.

    8. Zhou and Perleman (2007) J. Vir. 81(2):568.

    9. Kamath et al. (2005) J. Immunol. 174(2):767.

    Documentation

    Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
    12401-1 CoA

    12401-1 Certificate of Analysis (CoA)

    12401 SDS

    12401 Safety Data Sheet