Rhesus/Cynomolgus IFN Alpha 2

Product Features: 
  • Recombinant Rhesus/Cynomolgus IFN Alpha 2 expressed in E.coli

Ordering

Catalog No. Pack Size Price Quantity
14110-1

1 x 105 units

$295.00

Specifications

Formulation Supplied frozen with a carrier protein
Molecular Weight 19.5 kDa
Source cDNA expressed in E.coli
Purity > 98% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using cytopathic effect inhibition assays on Bovine (MDBK) cells with VSV and Human (A549) cells with EMCV
The EC50 for bovine IFN is ~ 5 U/ml
The EC50 for human IFN is ~ 1 U/ml.
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Accession Number XM_001107516

Tech Info / Data

Rhesus/Cynomolgus IFN Alpha 2 (14110) and Human IFN Alpha A Activity on Human A549 Cells Challenged with EMCV
Background: 

The cDNA for Rheus/Cynomolgus IFN-α2 was cloned from the Rhesus cell line LLC-MK2. This was found to be identical to the putative Rhesus IFN-α2 (XM_001107516) identified as part of the Rhesus genome project. There are 14 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha A (2a) sequence. There are 13 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha 2 (2b) sequence. This is in accord with a previous report [Villinger et al. (1995) J. Immunol. 155:3946] stating that there are 15-16 amino acids different between the Rhesus IFN alpha 2 and human alpha 2 (2b) and human alpha A(2a) respectively.

This Rhesus (Macaca mulatta) sequence is also identical to a Cynomolgus (Macaca fascicularis) IFN-α2 cloned from cDNA isolated from primary hepatocytes (PBL unpublished observation).

Citations

Activity of Rhesus/Cynomolgus IFN Alpha 2 (14110) and Human IFN Alpha A on an NHP cell line challenged with VSV in a CPE

Rhesus/Cynomolgus IFN Alpha 2 (14110) and Human IFN Alpha A antiviral activities on an NHP cell line with VSV in a CPE

Results are representative and may vary depending upon experimental conditions.

Related Products

Catalog No. Description Pack Size Price Quantity
46100-1 VeriKine Cynomolgus/Rhesus Interferon Alpha Serum ELISA Kit
Compatibility: Serum, Plasma, Tissue Culture Media
Assay Range: 25-1600 pg/ml

1 x 96-well plate

$785.00

Find your product of interest by searching for category, species, molecule and/or application.

Visit our blog to read about recent developments in the fields of interferon, cytokine and biomarker research!

Sign up for our mailing list to be kept up-to-date on the latest promotions, company and product news.

Go to top