Rhesus/Cynomolgus IFN Alpha 2

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Product Features: 
  • Recombinant Rhesus/Cynomolgus IFN Alpha 2 expressed in E.coli

Ordering

Catalog No. Pack Size Price Quantity
14110-1

1 x 105 units

 $295.00

Specifications

Formulation Supplied frozen with a carrier protein
Molecular Weight 19.5 kDa
Source cDNA expressed in E.coli
Purity > 98% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using cytopathic effect inhibition assays on Bovine (MDBK) cells with VSV and Human (A549) cells with EMCV
The EC50 for bovine IFN is ~ 5 U/ml
The EC50 for human IFN is ~ 1 U/ml.
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Accession Number XM_001107516

Tech Info / Data

Rhesus/Cynomolgus IFN Alpha 2 (14110) and Human IFN Alpha A Activity on Human A549 Cells Challenged with EMCV
Background: 

The cDNA for Rheus/Cynomolgus IFN-α2 was cloned from the Rhesus cell line LLC-MK2. This was found to be identical to the putative Rhesus IFN-α2 (XM_001107516) identified as part of the Rhesus genome project. There are 14 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha A (2a) sequence. There are 13 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha 2 (2b) sequence. This is in accord with a previous report [Villinger et al. (1995) J. Immunol. 155:3946] stating that there are 15-16 amino acids different between the Rhesus IFN alpha 2 and human alpha 2 (2b) and human alpha A(2a) respectively.

This Rhesus (Macaca mulatta) sequence is also identical to a Cynomolgus (Macaca fascicularis) IFN-α2 cloned from cDNA isolated from primary hepatocytes (PBL unpublished observation).

Citations

3 Citations:

  1. Mudd, Joseph, et al. (2018). Hallmarks of Primate Lentiviral Immunodeficiency Infection Recapitulate Loss of Innate Lymphoid Cells. Nature Communications, 12 pgs. PMID: 30262807. (link)

  2. Wonderlich, Elizabeth, et al. (2015). Macrophages and Myeloid Dendritic Cells Lose T Cell-Stimulating Function in Simian Immunodeficiency Virus Infection Associated with Diminished IL-12 and IFN-alpha Production. Journal of Immunology, 9 pgs. PMID: 26297760. (link)

  3. Dudek, Arkadiusz, et al. (2007). First in Human Phase I Trial of 852A, a Novel Systemic Toll-like Receptor 7 Agonist, to Activate Innate Immune Responses in Patients with Advanced Cancer. Clinical Cancer Research, 8 pgs. PMID: 18056192. (link)

References:

  1. Villinger et al. (1995) J. Immunol. 155:3946.

Documentation

Activity of Rhesus/Cynomolgus IFN Alpha 2 (14110) and Human IFN Alpha A on an NHP cell line challenged with VSV in a CPE

Rhesus/Cynomolgus IFN Alpha 2 (14110) and Human IFN Alpha A antiviral activities on an NHP cell line with VSV in a CPE

Results are representative and may vary depending upon experimental conditions.

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