Powered by BiozRecombinant Rhesus/Cynomolgus Interferon Alpha 2 expressed in E.coli
Activity of Rhesus/Cynomolgus IFN-Alpha 2 (14110) and Human IFN-Alpha A on an NHP cell line challenged with VSV in a CPE. Results are representative and may vary depending upon experimental conditions.
Product Info
| Bioactivity | Measured using cytopathic effect inhibition assays on Bovine (MDBK) cells with VSV and Human (A549) cells with EMCV The EC50 for bovine IFN is ~ 5 U/ml The EC50 for human IFN is ~ 1 U/ml. |
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Documentation
Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
14110-1 CoA
14110-1 Certificate of Analysis (CoA)
14110 SDS
14110 Safety Data Sheet
| Formulation | Supplied frozen with a carrier protein |
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| Molecular Weight | 19.5 kDa |
| Source | cDNA expressed in E.coli |
| Purity | > 98% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
| Storage | For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles |
| Accession Number | XM_001107516 |
Background
The cDNA for Rheus/Cynomolgus IFN-Alpha 2 was cloned from the Rhesus cell line LLC-MK2. This was found to be identical to the putative Rhesus IFN-Alpha 2 (XM_001107516) identified as part of the Rhesus genome project. There are 14 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha A (2a) sequence. There are 13 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha 2 (2b) sequence. This is in accord with a previous report [Villinger et al. (1995) J. Immunol. 155:3946] stating that there are 15-16 amino acids different between the Rhesus IFN-Alpha 2 and human alpha 2 (2b) and human alpha A(2a) respectively.
This Rhesus (Macaca mulatta) sequence is also identical to a Cynomolgus (Macaca fascicularis) IFN-Alpha 2 cloned from cDNA isolated from primary hepatocytes (PBL unpublished observation).