Mouse IFN Beta, E.coli

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Product Features: 
  • E.coli expressed Mouse IFN Beta protein with a single non-glycosylated polypeptide chain
  • Increased stability with engineered disulfide bond
  • 95% or greater purity

Ordering

Catalog No. Pack Size Price Quantity
12400-1

1 x 105 units

 $495.00

Specifications

Formulation Supplied frozen in 20mM HEPES, pH 6.0; 0.5M NaCl; 6% glycerol; 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.6 kDa
Source Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" (J. Interferon Res. 12: 139-43).
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV.
EC50 for IFNβ is ~ 2.5U/ml
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms Mouse Beta IFN, Mouse Beta Interferon, Mouse IFN Beta, Mouse IFNB, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta

Tech Info / Data

Background: 

Interferon Beta (IFN Beta) is generally the first Type I IFN to be expressed after viral infection. In the mouse both IFN Beta and IFN Alpha 4 prime cells for the production of the other Type I IFNs. Mouse IFN Beta was originally cloned by Higashi et al. [(1983) J. Biol. Chem. 258(15):9522] and has been engineered to contain a disulfide which confers added stability. 

Citations

53 Citations:

  1. Panagioti, E., et al., (2021), Immunostimulatory bacterical antigen-armed oncolytic measles virotherapy significantly increases the potency of anti-PD1 checkpoint therapy, J.Clin. Invest., 131(10):e141614, DOI: 10.1172/JCI141614 (link)
  2. Ma, Hongming, et al. (2020). Barrier-to-Autointegration Factor 1 Protects Against a Basal cGAS-STING Response. mBio, 15 pgs. PMID: 32156810. (link)

  3. Platanitis, et al. (2019). A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription. Nature Communications, 17 pgs. PMID: 31266943. (link)

  4. Neil, Jessica, et al. (2019). IFN-I and IL-22 mediate protective effects of intestinal viral infection. Nature Microbiology, 16 pgs. PMID: 31182797. (link)

  5. Markosyan, Nune, et al. (2019). Tumor cell-intrinsic EPHA2 suppresses anti-tumor immunity by regulating PTGS2 (COX-2). JCI, 40 pgs. PMID: 31162144. (link)

  6. Wang, Andrew, et al. (2019). Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice. PNAS. PMID: 30674681. (link)

  7. Marro, Brett, et al. (2019). Macrophage IFN-I signaling promotes autoreactive T cell infiltration into islets in type 1 diabetes model. JCI Insight, 13 pgs. PMID: 30674713. (link)

  8. Chen, et al. (2019). Sephin1, which prolongs the integrated stress response, is a promising therapeutic for multiple sclerosis. Brain, 18 pgs. PMID: 30657878. (link)

  9. Michalicka, Matthew (2019). COMBINATION IMMUNOTHERAPY WITH INHIBITOR OF APOPTOSIS (IAP) ANTAGONISTS TO TREAT NEUROBLASTOMA. University of Ottawa, 153 pgs. PMID: no PMID.

  10. Li, et al. (2018). The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING. CellPress. PMID: 30566866. (link)

  11. Sharma, Deepika, et al. (2018). TNF/TNFR Axis Promotes Pyrin Inflammasome Activation and Distinctly Modulates Pyrin Inflammasomopathy. JCI , 14 pgs. PMID: 30457980. (link)

  12. Takahashi, Akiko, et al. (2018). Downregulation of Cytoplasmic DNases is Implicated in Cytoplasmic DNA Accumulation and SASP in Senescent Cells. Nature Communications, 12 pgs. PMID: 29593264.  (link)

  13. Cui, Huachun, et al. (2018). IFN Regulatory Factor 2 Inhibits Expression of Glycolytic Genes and Lipopolysaccharide-Induced Proinflammatory Responses in Macrophage. Journal of Immunology, 27 pgs. PMID: 29563175. (link)

  14. Kim, Kyoung Min, et al. (2018). Interferon beta Protects Against Avascular Osteonecrosis Through Interleukin 6 Inhibition and Silent Information Regulator Transcript-1 Upregulation. Oncotarget, 14 pgs. PMID: 29423066. (link)

  15. Elias, Salah, et al. (2018). Blimp-1/PRDM1 is a Critical Regulator of Type III Interferon Responses in Mammary Epithelial Cells. Scientific Reports, 11 pgs. PMID: 29321612. (link)

  16. Zhu, Qifan (2018). Roles of Cytosolic Nucleic Acid Sensors in Cancer and Infection. UTHSC, 107 pgs. PMID: no PMID.

  17. Case, James (2018). Roles of the Coronavirus 3'-to-5' Exoribonuclease and N7-Methyltransferase in Counteracting Innate Immunity. Vanderbilt University, 211 pgs. PMID: no PMID. (link)

  18. McDonough, Ashley, et al. (2017). Ischemia/Reperfusion Induces Interferon-Stimulated Gene Expression in Microglia. Journal of Neuroscience, 17 pgs. PMID: 28747383. (link)

  19. Nakaya, Yuki, et al. (2017). AIM2-Like Receptors Positively and Negatively Regulate the Interferon Response Induced by Cytosolic DNA. mBio, 17 pgs. PMID: 28679751. (link)

  20. Stier, Matthew T, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology, 25 pgs. PMID: 28576981. (link)

  21. Beug, Shawn, et al. (2017). Smac Mimetics Synergize with Immune Checkpoint Inhibitors to Promote Tumour Immunity Against Glioblastoma. Nature Communications, 15 pgs. PMID: 28198370. (link)

  22. Rahman, Masmudur, et al. (2017). Myxoma Virus dsRNA Binding Protein M029 Inhibits the Type I IFN-Induced Antiviral State in a Highly Species-Specific Fashion. Viruses, 22 pgs. PMID: 28157174. (link)

  23. Labzin, Larisa I, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)

  24. Onabajo, Olusegun, et al. (2015). Expression of Interferon Lambda 4 is Associated with Reduced Proliferation and Increased Cell Death in Human Hepatic Cells. Journal of Interferon & Cytokine Research, 13 pgs. PMID: 26134097. (link)

  25. Majumdar, Tanmay, et al. (2015). Induction of interferon-stimulated genes by IRF3 promotes replication of Toxoplasma gondii. PLOS Pathogens, 22 pgs. PMID: 25811886. (link)

  26. Foxman, Ellen, et al. (2015). Temperature-Dependent Innate Defense Against the Common Cold Virus Limits Viral Replication at Warm Temperature in Mouse Airway Cells. PNAS, 6 pgs. PMID: 25561542. (link)

  27. Welsby, Iain, et al. (2014). PARP12, an interferon-stimulated gene involved in the control of protein translation and inflammation. JBC, 17 pgs. PMID: 25086041. (link)

  28. Duong, Sean, et al. (2014). Polymicrobial sepsis alters antigen-dependent and -independent memory CD8 T cell functions. Journal of Immunology, 20 pgs. PMID: 24646738. (link)

  29. Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)

  30. Taylor, Juliet, et al. (2014). Type I Interferon Signaling Mediates Neuro-Inflammatory Events in Model of Alzheimer's Disease. Neurobiology of Aging, 12 pgs. PMID: 24262201. (link)

  31. Low-Calle, Ana Maria, et al. (2014). Characterization of a Murine Neuron-Enriched Model of Primary Trigeminal Ganglia Cultures to Study the Interferon-beta Antiviral Effect Against Herpes Simplex Virus Type 1. Infectio, 8 pgs. PMID: no PMID. (link)

  32. Munitic, Ivana, et al. (2013). Optineurin insufficiency impairs IRF3 but not NF-κB activation in immune cells. Journal of Immunology, 23 pgs. PMID: 24244017. (link)

  33. Li, et al. (2013). A Short Hairpin RNA Screen of Interferon-Stimulated Genes Identifies a Novel Negative Regulator of the Cellular Antiviral Response. mBio, 11 pgs. PMID: 23781071. (link)

  34. Gandhapudi, et al. (2013). TRIF is Required for TLR4 Mediated Adjuvant Effects on T Cell Clonal Expression. PLOS One, 11 pgs. PMID: 23457630. (link)

  35. Chen, Xuefen, et al. (2012). Requirement for the Histone Deacetylase Hdac3 for the Inflammatory Gene Expression Program in Macrophages. PNAS, 10 pgs. PMID: 22802645. (link)

  36. Freeman, Bailey E, et al. (2012). Regulation of innate CD8+ T-cell activation mediated by cytokines. PNAS, 6 pgs. PMID: 22665806. (link)

  37. Sutherland, et al. (2011). Evaluating vaccinia virus cytokine co-expression in TLR GKO mice. Immunology and Cell Biology. PMID: 21173782. (link)

  38. Leu, Shaw-Wei, et al. (2011). TLR4 through IFN-β promotes low molecular mass hyaluronan-induced neutrophil apoptosis. Journal of Immunology, 8 pgs. PMID: 21098223. (link)

  39. Li, et al. (2010). IFN-alpha Expression and Antiviral Effects are Subtype and Cell Type Specific in the Cardiac Response to Viral Infection. JVI, 22 pgs. PMID: 19896686. (link)

  40. Chang, Tsung-Hsien, et al. (2009). Ebola Zaire Virus Blocks Type I Interferon Production by Exploiting the Host SUMO Modification Machinery. PLOS Pathogens, 15 pgs. PMID: 19557165. (link)

  41. Trilling, Mirko, et al. (2009). Gamma Interferon-Induced Interferon Regulatory Factor 1-Dependent Antiviral Response Inhibits Vaccinia Virus Replication in Mouse but Not Human Fibroblasts. JVI, 36 pgs. PMID: 19211768. (link)

  42. Yamada, Atsushi, et al. (2009). IFN-gamma down-regulates Secretoglobin 3A1 gene expression. National Institute of Health, 9 pgs. PMID: 19135978. (link)

  43. Richez, Christophe, et al. (2009). TLR4 Ligands Induce IFN-alpha Production by Mouse Conventional Dendritic Cells and Human Monocytes after IFN-beta Priming. Journal of Immunology, 10 pgs. PMID: 19124725. (link)

  44. Capraro, et al. (2008). Virus Growth and Antibody Responses Following Respiratory Tract Infection of Ferrets and Mice with WT and P/V Mutants of the Paramyxovirus Simian Virus 5. JVI, 26 pgs. PMID: 18456301. (link)

  45. Guo, Beichu, et al. (2008). The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. JCI, 11 pgs. PMID: 18382764. (link)

  46. Molle, Celine, et al. (2007). IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3. Journal of Immunology, 10 pgs. PMID: 17548596. (link)

  47. O'Connell, Ryan, et al. (2007). MicroRNA-155 is induced during the macrophage inflammatory response. PNAS, 6 pgs. PMID: 17242365. (link)

  48. Zhou, Haixia, et al. (2007). Mouse Hepatitis Virus Does Not Induce Beta Interferon Synthesis and Does Not Inhibit Its Induction by Double-Stranded RNA. JVI, 7 pgs. PMID: 17079305. (link)

  49. Kamphuis, Elisabeth, et al. (2006). Type I interferons directly regulate lymphocyte recirculation and cause transient blood lymphopenia. Blood Journal, 10 pgs. PMID: 16868248. (link)

  50. Jaini, et al. (2006). Gene-Based Intramuscular Interferon-beta Therapy for Experimental Autoimmune Encephalomyelitis. Molecular Therapy, 7 pgs. PMID: 16782409. (link)

  51. Braun, Benjamin S, et al. (2006). Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood Journal. PMID: 16720837. (link)

  52. Zhang, Fahao, et al. (2006). Differential growth of IFN-beta-engineered tumor cells in nude and IFN receptor-null mice. Journal of Interferon & Cytokine Research. PMID: 16487031. (link)

  53. Pejawar, Sharmila, et al. (2005). Abortive versus Productive Viral Infection of Dendritic Cells with a Paramyxovirus Results in Differential Upregulation of Select Costimulatory Molecules. JVI, 14 pgs. PMID: 15919909. (link)

References: (For additional references, please refer to the Certificate of Analysis)

  1. Mesplede et al. (2003) Autoimmunity 36(8):447.

  2. Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

  3. Higashi et al. (1983) J. Biol. Chem. 258(15):9522.

  4. Hayashi et al. (2002) J. Immunol. 277(31):27880.

  5. Fujimura et al. (2006) Infect. Immu. 75(5);254.
    1.  

Documentation

Antiviral Activities of PBL's Carrier-containing (12400) and Carrier-free Mouse IFN Beta (12401) on Mouse L929 cells infected with EMCV

Image depicting the Antiviral Activities of PBL's Carrier-containing and Carrier-free Mouse Interferon Beta as determined in a L929/EMCV cytopathic effect (CPE) assay

The activity of Mouse IFN Beta with carrier (PBL 12400) and Mouse IFN beta, carrier-free (PBL 12401) was compared in the L929/EMCV CPE assay. The EC50 for Mouse IFN Beta in this experiment was 1.8 U/ml while the EC50 for Mouse IFN Beta (CF) was 1.5 U/ml when calibrated to the international standard.

Results are representative and may vary depending upon experimental conditions.

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