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Mouse IFN-Beta, E.Coli

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Mouse IFN-Beta, E.Coli

Catalog Number: 12400

Often-cited E.coli-expressed Mouse IFN-Beta protein with a single non-glycosylated polypeptide chain. This protein boasts increased stability with an engineered disulfide bond.


Pack Size
Product Info

Bioactivity Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV.
EC50 for IFNβ is ~ 2.5U/ml


Formulation Supplied frozen in 20mM HEPES, pH 6.0; 0.5M NaCl; 6% glycerol; 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.6 kDa
Source Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" (J. Interferon Res. 12: 139-43).
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms Mouse Beta-IFN, Mouse Beta Interferon, Mouse IFN-Beta, Mouse IFNB, Mouse Type I IFN-Beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta

Tech Info & Data



IFN-Beta is generally the first Type I IFN to be expressed after viral infection. In the mouse both IFN-Beta and IFN-Alpha 4 prime cells for the production of the other Type I IFNs. Mouse IFN-Beta was originally cloned by Higashi et al. [(1983) J. Biol. Chem. 258(15):9522] and has been engineered to contain a disulfide which confers added stability. 


57 Citations


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  23. Nakaya, Yuki, et al. (2017). AIM2-Like Receptors Positively and Negatively Regulate the Interferon Response Induced by Cytosolic DNA. mBio, 17 pgs. PMID: 28679751. (link)

  24. Stier, Matthew T, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology, 25 pgs. PMID: 28576981. (link)

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  27. Labzin, Larisa I, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)

  28. Onabajo, Olusegun, et al. (2015). Expression of Interferon Lambda 4 is Associated with Reduced Proliferation and Increased Cell Death in Human Hepatic Cells. Journal of Interferon & Cytokine Research, 13 pgs. PMID: 26134097. (link)

  29. Majumdar, Tanmay, et al. (2015). Induction of interferon-stimulated genes by IRF3 promotes replication of Toxoplasma gondii. PLOS Pathogens, 22 pgs. PMID: 25811886. (link)

  30. Foxman, Ellen, et al. (2015). Temperature-Dependent Innate Defense Against the Common Cold Virus Limits Viral Replication at Warm Temperature in Mouse Airway Cells. PNAS, 6 pgs. PMID: 25561542. (link)

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  32. Duong, Sean, et al. (2014). Polymicrobial sepsis alters antigen-dependent and -independent memory CD8 T cell functions. Journal of Immunology, 20 pgs. PMID: 24646738. (link)

  33. Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)

  34. Taylor, Juliet, et al. (2014). Type I Interferon Signaling Mediates Neuro-Inflammatory Events in Model of Alzheimer's Disease. Neurobiology of Aging, 12 pgs. PMID: 24262201. (link)

  35. Low-Calle, Ana Maria, et al. (2014). Characterization of a Murine Neuron-Enriched Model of Primary Trigeminal Ganglia Cultures to Study the Interferon-beta Antiviral Effect Against Herpes Simplex Virus Type 1. Infectio, 8 pgs. PMID: no PMID. (link)

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  43. Li, et al. (2010). IFN-alpha Expression and Antiviral Effects are Subtype and Cell Type Specific in the Cardiac Response to Viral Infection. JVI, 22 pgs. PMID: 19896686. (link)

  44. Chang, Tsung-Hsien, et al. (2009). Ebola Zaire Virus Blocks Type I Interferon Production by Exploiting the Host SUMO Modification Machinery. PLOS Pathogens, 15 pgs. PMID: 19557165. (link)

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  46. Yamada, Atsushi, et al. (2009). IFN-gamma down-regulates Secretoglobin 3A1 gene expression. National Institute of Health, 9 pgs. PMID: 19135978. (link)

  47. Richez, Christophe, et al. (2009). TLR4 Ligands Induce IFN-alpha Production by Mouse Conventional Dendritic Cells and Human Monocytes after IFN-beta Priming. Journal of Immunology, 10 pgs. PMID: 19124725. (link)

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  2. Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

  3. Higashi et al. (1983) J. Biol. Chem. 258(15):9522.

  4. Hayashi et al. (2002) J. Immunol. 277(31):27880.

  5. Fujimura et al. (2006) Infect. Immu. 75(5);2544.

  6. Jaini, et al. (2006) Mol. Ther. 14(3):416.

  7. Zhou and Perleman (2007) J. Vir. 81(2):568.

  8. Kamath, et al. (2005) J. Immunol. 174(2):767.

  9. Day, et al. (1992) J. Interferon Res. 12:139-43.


Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
12400-1 CoA

12400-1 Certificate of Analysis (CoA)

12400 SDS

12400 Safety Data Sheet