Mouse IFN Beta, E.coli

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Product Features: 
  • E.coli expressed Mouse IFN Beta protein with a single non-glycosylated polypeptide chain
  • Increased stability with engineered disulfide bond
  • 95% or greater purity

Ordering

Catalog No. Pack Size Price Quantity
12400-1

1 x 105 units

 $495.00

Specifications

Formulation Supplied frozen in 20mM HEPES, pH 6.0; 0.5M NaCl; 6% glycerol; 0.1% Bovine Serum Albumin (BSA)
Molecular Weight 19.6 kDa
Source Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" (J. Interferon Res. 12: 139-43).
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV.
EC50 for IFNβ is ~ 2.5U/ml
Storage For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms Mouse Beta IFN, Mouse Beta Interferon, Mouse IFN Beta, Mouse IFNB, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta

Tech Info / Data

Background: 

Interferon Beta (IFN Beta) is generally the first Type I IFN to be expressed after viral infection. In the mouse both IFN Beta and IFN Alpha 4 prime cells for the production of the other Type I IFNs. Mouse IFN Beta was originally cloned by Higashi et al. [(1983) J. Biol. Chem. 258(15):9522] and has been engineered to contain a disulfide which confers added stability. 

Citations

Citations:

  1. A Short Hairpin RNA Screen of Interferon-Stimulated Genes Identifies a Novel Negative Regulator of the Cellular Antiviral Response. Li et al., 2013, mBio4(3):doi: 10.1128/mBio.00385-13 (link)

  2. TRIF Is Required for TLR4 Mediated Adjuvant Effects on T Cell Clonal Expression. Gandhapudi et al., 2013, PLoS One 8(2):e56855 (link)

  3. IFN-α expression and antiviral effects are subtype and cell type specific in the cardiac response to viral infection. Li and Sherry, 2010, J. Virol., 396:59 (link) 

  4. Virus growth and antibody responses following respiratory tract infection of ferrets and mice with WT and P/V mutants of the paramyxovirus Simian Virus 5. Capraro et al., 2008, J.Virol., 376:418 (link)

  5. Gene-Based Intramuscular Interferon-β Therapy for Experimental Autoimmune Encephalomyelitis., Jaini et al., 2006, Mol. Ther., 14(3):416 (link)

References: (For additional references, please refer to the Certificate of Analysis)

  1. Mesplede et al. (2003) Autoimmunity 36(8):447.

  2. Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

  3. Higashi et al. (1983) J. Biol. Chem. 258(15):9522.

  4. Hayashi et al. (2002) J. Immunol. 277(31):27880.

  5. Fujimura et al. (2006) Infect. Immu. 75(5);2544.

Documentation

Antiviral Activities of PBL's Carrier-containing (12400) and Carrier-free Mouse IFN Beta (12401) on Mouse L929 cells infected with EMCV

Image depicting the Antiviral Activities of PBL's Carrier-containing and Carrier-free Mouse Interferon Beta as determined in a L929/EMCV cytopathic effect (CPE) assay

The activity of Mouse IFN Beta with carrier (PBL 12400) and Mouse IFN beta, carrier-free (PBL 12401) was compared in the L929/EMCV CPE assay. The EC50 for Mouse IFN Beta in this experiment was 1.8 U/ml while the EC50 for Mouse IFN Beta (CF) was 1.5 U/ml when calibrated to the international standard.

Results are representative and may vary depending upon experimental conditions.

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