A cost-effective and performance-enhancing alternative is to employ an antibody mixture consisting of anti-human IFNAR2 monoclonal antibody, anti-human IFN alpha polyclonal antibody, and anti-human IFN beta polyclonal antibody (39000-1). The inclusion of the two polyclonals reduces the amount of anti-human IFNAR2 monoclonal antibody required and improves neutralization of multiple Type I IFNs often found in complex samples.
A human cell line responsding to all Type I IFNs was used to measured IFN activity.
The anti-IFN-alpha antibody also exhibits cross-reactivity with IFN-omega.
The anti-IFN-beta exhibited no cross-reactivity with IFN-alpha. IFN pool included IFN alpha, beta, and omega.
The anti-IFNAR2 binds to the receptor, effectively neutralizing all Type I IFNs.
References:
1. Schijf et al., PLOS ONE, 2013, 8(11):e81695
2. Davis et al., J. Immunol., 2008, 181:8204
3. Nestle et al., JEM, 2005, 202(1):135
Keywords:
Human Type I IFNs neutralization
Type 1 IFN neutralizing antibodies
IFNAR2
Multiple Type interferons
anti-IFN
A cost-effective and performance-enhancing alternative is to employ an antibody mixture consisting of anti-human IFNAR2 monoclonal antibody, anti-human IFN alpha polyclonal antibody, and anti-human IFN beta polyclonal antibody (39000-1). The inclusion of the two polyclonals reduces the amount of anti-human IFNAR2 monoclonal antibody required and improves neutralization of multiple Type I IFNs often found in complex samples.
A human cell line responsding to all Type I IFNs was used to measured IFN activity.
The anti-IFN-alpha antibody also exhibits cross-reactivity with IFN-omega.
The anti-IFN-beta exhibited no cross-reactivity with IFN-alpha. IFN pool included IFN alpha, beta, and omega.
The anti-IFNAR2 binds to the receptor, effectively neutralizing all Type I IFNs.
References:
1. Schijf et al., PLOS ONE, 2013, 8(11):e81695
2. Davis et al., J. Immunol., 2008, 181:8204
3. Nestle et al., JEM, 2005, 202(1):135
Keywords:
Human Type I IFNs neutralization
Type 1 IFN neutralizing antibodies
IFNAR2
Multiple Type interferons
anti-IFN
