Human IFN-Alpha Multi-Subtype ELISA Kit (Serum, Plasma, TCM)
Catalog Number: 41110
This Human IFN-Alpha ELISA kit detects 11 of 12 major IFN-Alpha subtypes in serum, plasma, and tissue culture media (TCM).
|Matrix Compatibility||Serum, Plasma, Tissue Culture Media|
|Assay Range||12.5 - 1000 pg/ml|
|Assay Length||3 hours, 15 minutes|
|Specificity||Human Interferon Alpha|
The VeriKine Human IFN-Alpha Multi-Subtype Serum ELISA kit is designed to provide accurate measurement of global human IFN-Alpha amounts in serum and plasma samples. This kit quantitates human IFN-Alpha in human sera, plasma and tissue culture media using a sandwich immunoassay. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health.
*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).
*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).
|CVs and Spike Recovery||
Inter-Assay: ≤ 8%
No cross-reactivity against
|Synonyms||IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha|
|Expiration Date||Twenty-one months from the date of manufacture|
|Shipping Condition||Wet Ice|
- Pre-coated microtiter plate(s)
- Plate Sealers
- Wash Solution Concentrate
- Human IFN-Alpha Standard, 10,000 pg/ml
- Sample Diluent
- Antibody Concentrate
- HRP Conjugate Concentrate
- Concentrate Diluent
- TMB Substrate
- Stop Solution
- Dilution Buffer
Additional Materials Required (Not Provided)
- Microplate reader capable of reading an OD at a wavelength of 450 nm
- Variable volume microtiter pipettes
- Adjustable multichannel pipette (50-300 μl)
- Reagent reservoirs
- Wash bottle or plate washing system
- Distilled or deionized water
- Serological pipettes (1, 5, 10 or 25 ml)
- Disposable pipette tips (polypropylene)
- Plate shaker
Tech Info & Data
Tips, Tools and Troubleshooting:
Spike Recovery of IFN-Alpha in Different Matrices
|Low (30 pg/ml)||Medium (300 pg/ml)||High (800 pg/ml)|
|Normal Human Serum||94%||100%||96%|
Normal Human Plasma
Normal Human Plasma
Normal Human Plasma
Interferons (IFNs) are a family of cytokines produced by macrophages, neutrophils, dendritic cells and other somatic cells in response to viruses and pathogens. Interferon Alpha (IFN-alpha), a type I interferon, was one of the first FDA-approved biotherapeutic treatments. Additionally, there is a growing amount of evidence suggesting IFN-alpha may be involved in certain autoimmune diseases such as Systemic Lupus Erythematosus (SLE). Accurate and reproducible detection of IFN-alpha in human serum or plasma plays an important role in advancing immunological investigation and drug discovery.
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