Human IFN Alpha Multi-Subtype ELISA Kit (Serum, Plasma, TCM)

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Product Features: 
  • Human IFN-Alpha ELISA kit for measuring global human interferon alpha amount in serum, plasma, and tissue culture media (TCM)
  • Greater than 90% spike recovery in normal human serum
  • ELISA assay detects 11 of 12 major human IFN-alpha subtypes
  • Less than 10% inter and intra-assay CV

Full Product Name: VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA Kit

 

Looking for a TCM compatible All Subtype ELISA? Check out our NEW 41135 kit!

Ordering

Catalog No. Pack Size Price Quantity
41110-1

1 x 96-well plate

 $695.00
41110-2

5 x 96-well plate

 $2,825.00
$2,995.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 12.5 - 1000 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Alpha (No cross-reactivity against human IFN-γ, IFN-β or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; bovine IFN-τ.)
Synonyms: IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
 
Inter-Assay CV: ≤ 8%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 90% in Serum
 
Storage: 2-8°C
Expiration Date: Twenty-one months from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Sample Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • TMB Substrate 
  • Stop Solution
  • Dilution Buffer

Tech Info / Data

IFN alpha 2a spike recovery from human serum and plasma using PBL Human Interferon Alpha Multi-Subtype Serum ELISA (41110)
Background: 

The VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA kit is designed to provide accurate measurement of global human IFN-alpha amount in serum and plasma samples. This kit quantitates human IFN-alpha in human sera, plasma and tissue culture media using a sandwich immunoassay. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health. This kit can detect 14 different human IFN-alpha subtypes/alleles providing a better view of IFN-alpha production in a sample analysis. 

Interferons (IFNs) are a family of cytokines produced by macrophage, neutrophils, dendritic cells and other somatic cells in response to viruses and pathogens. Interferon Alpha (IFN-alpha), a type I interferon, was one of the first FDA approved biotherapeutic treatments. Additionally, there is a growing amount of evidence suggesting IFN-alpha may be involved in certain autoimmune diseases such as Systemic Lupus Erythematosus (SLE). Accurate and reproducible detection of IFN-alpha in human serum or plasma plays an important role in advancing immunological investigation and drug discovery. 

Citations

18 Citations:

  1. Ding, Siyuan, et al. (2018). STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection. Nature, 8 pgs. PMID: 29662124. (link)

  2. Oka, Takakazu, et al. (2018). Changes in Fatigue, Autonomic Functions, and Blood Biomarkers Due to Sitting Isometric Yoga in Patients with Chronic Fatigue Syndrome. BioPsychoSocial Medicine, 11 pgs. PMID: 29643935. (link)

  3. Xi, Yang, et al. (2015). Rhinovirus Stimulated IFN-alpha Production: How Important Are Plasmacytoid DCs, Monocytes, and Endosomal pH?. Clinical & Translational Immunology, 8 pgs. PMID: 26682054. (link)

  4. Pellerin, Alex, et al. (2015). Anti-BDCA2 Monoclonal Antibody Inhibits Plasmacytoid Dendritic Cell Activation Through Fc-Dependent and Fc-Independent Mechanisms. EMBO Molecular Medicine, 14 pgs. PMID: 25762615. (link)

  5. Liu, Li, et al. (2012). Influenza A virus induces interleukin-27 through cyclooxygenase-2 and protein kinase A signaling. JBC, 13 pgs. PMID: 22343630. (link)

  6. Cai, Yongming, et al. (2011). Pharmacokinetics, tissue distribution, excretion, and antiviral activity of pegylated recombinant human consensus interferon-α variant in monkeys, rats and guinea pigs. Regulatory Peptides, 8 pgs. PMID: 21985916. (link)

  7. Liao, Anne, et al. (2011). Interferon β is associated with type 1 interferon-inducible gene expression in dermatomyositis. Annals of the Rheumatic Disease, 6 pgs. PMID: 21177291. (link)

  8. Van Brussel, et al. (2011). Decreased Numbers of Peripheral Blood Dendritic Cells in Patients with Coronary Artery Disease are Associated with Diminished Plasma Flt3 Ligand Levels and Impaired Plasmacytoid Dendritic Cell Function. Clinical Science, 11 pgs. PMID: 21143200. (link)

  9. Traore, Abdel, et al. (2011). Impact of Low Dose Naltrexone (LDN) on Antiretroviral Therapy (ART) Treated HIV+ Adults in Mali: A Single Blind Randomized Clinical Trial. Journal of AIDS and HIV Research, 9 pgs. PMID: no PMID. (link)

  10. Meythaler, Mareike, et al. (2009). Differential CD4+ T-Lymphocyte Apoptosis and Bystander T-Cell Activation in Rhesus Macaques and Sooty Mangabeys during Acute Simian Immunodeficiency Virus Infection. JVI, 12 pgs. PMID: 18987149. (link)

  11. Yu, Xue-Lian, et al. (2008). Measles Virus Infection in Adults Induces Production of IL-10 and is Associated with Increased CD4+ CD25+ Regulatory T Cells. Journal of Immunology, 12 pgs. PMID: 18981159. (link)

  12. Park, SH, et al. (2008). Codelivery of PEG-IFN-alpha inhibits HCV DNA vaccine-induced T cell responses but not humoral responses in African green monkeys. Vaccine, 6 pgs. PMID: 18556097. (link)

  13. Duan, Hangjun, et al. (2008). Combined analysis of monocyte and lymphocyte messenger RNA expression with serum protein profiles in patients with scleroderma. Arthritis and Rheumatism, 10 pgs. PMID: 18438864. (link)

  14. Cheng, Gong, et al. (2007). Alpha interferon is a powerful adjuvant for a recombinant protein vaccine against foot-and-mouth disease virus in swine, and an effective stimulus of in vivo immune response. Vaccine. PMID: 17555848. (link)

  15. Masci, P, et al. (2007). Gene modulatory effects, pharmacokinetics, and clinical tolerance of interferon-alpha1b: a second member of the interferon-alpha family. Clinical Pharmacology and Therapeutics, 8 pgs. PMID: 17339865. (link)

  16. Yoshikawa, et al (2006). Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma. Journal of Neuroimmunology. PMID: 16574246. (link)

  17. Wang, Yichuan, et al. (2005). The Toll-Like Receptor 7 (TLR7) Agonist, Imiquimod, and the TLR9 Agonist, CpG ODN, Induce Antiviral Cytokines and Chemokines but Do Not Prevent Vaginal Transmission of Simian Immunodeficiency Virus When Applied Intravaginally to Rhesus Macaques. JVI, 16 pgs. PMID: 16254370. (link)

  18. Yang, Jia Jin, et al. (2004). Circumvention of normal constraints on granule protein gene expression in peripheral blood neutrophils and monocytes of patients with antineutrophil cytoplasmic autoantibody-associated glomerulonephritis. JASN, 12 pgs. PMID: 15284296. (link)

  19.  

Documentation

Typical Interferon Alpha Standard Curve from 12.5 pg/ml to 1000 pg/ml in Standard Diluent Using PBL's Human IFN Alpha Multi-Subtype Serum ELISA (41110)

 

Human IFN alpha standard curve from 12.5 to 1000 pg/ml using PBL Human IFN Alpha Multi-Subtype Serum ELISA (41110)

Assay Range: 12.5 - 1000 pg/ml

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