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Human IFN-Alpha Multi-Subtype ELISA Kit (Serum, Plasma, TCM)

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Human IFN-Alpha Multi-Subtype ELISA Kit (Serum, Plasma, TCM)

Catalog Number: 41110

This Human IFN-Alpha ELISA kit detects 11 of 12 major IFN-Alpha subtypes in serum, plasma, and tissue culture media (TCM).

 

Product Name: VeriKine Human Interferon-Alpha Multi-Subtype ELISA Kit (Serum, Plasma,TCM)

$615.00

Pack Size
Product Info

Matrix Compatibility Serum, Plasma, Tissue Culture Media
Assay Range 12.5 - 1000 pg/ml
Assay Length 3 hours, 15 minutes
Specificity Human Interferon Alpha

 

The VeriKine Human IFN-Alpha Multi-Subtype Serum ELISA kit is designed to provide accurate measurement of global human IFN-Alpha amounts in serum and plasma samples. This kit quantitates human IFN-Alpha in human sera, plasma and tissue culture media using a sandwich immunoassay. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health. 

 

*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).

 

*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).

Specifications

CVs and Spike Recovery

Inter-Assay: ≤ 8%


Intra-Assay: ≤ 8%


Spike Recovery: ≥ 90% in Serum

Cross-reactivity

No cross-reactivity against

  • Human IFN-Gamma, IFN-Beta, IFN-Omega
  • Mouse or rat IFN-Alpha, IFN-Beta, IFN-Gamma
  • Bovine IFN-Tau
Synonyms IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
Storage 2-8°C
Expiration Date Twenty-one months from the date of manufacture
Shipping Condition Wet Ice

 

 

Materials Provided

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN-Alpha Standard, 10,000 pg/ml
  • Sample Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • TMB Substrate 
  • Stop Solution
  • Dilution Buffer

 

Additional Materials Required (Not Provided) 

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data

Tips, Tools and Troubleshooting:

 

Application Note:

 

 

Spike Recovery of IFN-Alpha in Different Matrices

  Low (30 pg/ml) Medium (300 pg/ml) High (800 pg/ml)
Normal Human Serum 94% 100% 96%

Normal Human Plasma

(Na-Citrate)

70% 112% 110%

Normal Human Plasma

(Na-Heparin)

95% 106%

101%

Normal Human Plasma

(Na-EDTA)

60% 86% 84%

 

 

Background

 

Interferons (IFNs) are a family of cytokines produced by macrophages, neutrophils, dendritic cells and other somatic cells in response to viruses and pathogens. Interferon Alpha (IFN-alpha), a type I interferon, was one of the first FDA-approved biotherapeutic treatments. Additionally, there is a growing amount of evidence suggesting IFN-alpha may be involved in certain autoimmune diseases such as Systemic Lupus Erythematosus (SLE). Accurate and reproducible detection of IFN-alpha in human serum or plasma plays an important role in advancing immunological investigation and drug discovery. 

 

Citations

18 Citations

 

  1. Ding, Siyuan, et al. (2018). STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection. Nature, 8 pgs. PMID: 29662124. (link)

  2. Oka, Takakazu, et al. (2018). Changes in Fatigue, Autonomic Functions, and Blood Biomarkers Due to Sitting Isometric Yoga in Patients with Chronic Fatigue Syndrome. BioPsychoSocial Medicine, 11 pgs. PMID: 29643935. (link)

  3. Xi, Yang, et al. (2015). Rhinovirus Stimulated IFN-alpha Production: How Important Are Plasmacytoid DCs, Monocytes, and Endosomal pH?. Clinical & Translational Immunology, 8 pgs. PMID: 26682054. (link)

  4. Pellerin, Alex, et al. (2015). Anti-BDCA2 Monoclonal Antibody Inhibits Plasmacytoid Dendritic Cell Activation Through Fc-Dependent and Fc-Independent Mechanisms. EMBO Molecular Medicine, 14 pgs. PMID: 25762615. (link)

  5. Liu, Li, et al. (2012). Influenza A virus induces interleukin-27 through cyclooxygenase-2 and protein kinase A signaling. JBC, 13 pgs. PMID: 22343630. (link)

  6. Cai, Yongming, et al. (2011). Pharmacokinetics, tissue distribution, excretion, and antiviral activity of pegylated recombinant human consensus interferon-α variant in monkeys, rats and guinea pigs. Regulatory Peptides, 8 pgs. PMID: 21985916. (link)

  7. Liao, Anne, et al. (2011). Interferon β is associated with type 1 interferon-inducible gene expression in dermatomyositis. Annals of the Rheumatic Disease, 6 pgs. PMID: 21177291. (link)

  8. Van Brussel, et al. (2011). Decreased Numbers of Peripheral Blood Dendritic Cells in Patients with Coronary Artery Disease are Associated with Diminished Plasma Flt3 Ligand Levels and Impaired Plasmacytoid Dendritic Cell Function. Clinical Science, 11 pgs. PMID: 21143200. (link)

  9. Traore, Abdel, et al. (2011). Impact of Low Dose Naltrexone (LDN) on Antiretroviral Therapy (ART) Treated HIV+ Adults in Mali: A Single Blind Randomized Clinical Trial. Journal of AIDS and HIV Research, 9 pgs. PMID: no PMID. (link)

  10. Meythaler, Mareike, et al. (2009). Differential CD4+ T-Lymphocyte Apoptosis and Bystander T-Cell Activation in Rhesus Macaques and Sooty Mangabeys during Acute Simian Immunodeficiency Virus Infection. JVI, 12 pgs. PMID: 18987149. (link)

  11. Yu, Xue-Lian, et al. (2008). Measles Virus Infection in Adults Induces Production of IL-10 and is Associated with Increased CD4+ CD25+ Regulatory T Cells. Journal of Immunology, 12 pgs. PMID: 18981159. (link)

  12. Park, SH, et al. (2008). Codelivery of PEG-IFN-alpha inhibits HCV DNA vaccine-induced T cell responses but not humoral responses in African green monkeys. Vaccine, 6 pgs. PMID: 18556097. (link)

  13. Duan, Hangjun, et al. (2008). Combined analysis of monocyte and lymphocyte messenger RNA expression with serum protein profiles in patients with scleroderma. Arthritis and Rheumatism, 10 pgs. PMID: 18438864. (link)

  14. Cheng, Gong, et al. (2007). Alpha interferon is a powerful adjuvant for a recombinant protein vaccine against foot-and-mouth disease virus in swine, and an effective stimulus of in vivo immune response. Vaccine. PMID: 17555848. (link)

  15. Masci, P, et al. (2007). Gene modulatory effects, pharmacokinetics, and clinical tolerance of interferon-alpha1b: a second member of the interferon-alpha family. Clinical Pharmacology and Therapeutics, 8 pgs. PMID: 17339865. (link)

  16. Yoshikawa, et al (2006). Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma. Journal of Neuroimmunology. PMID: 16574246. (link)

  17. Wang, Yichuan, et al. (2005). The Toll-Like Receptor 7 (TLR7) Agonist, Imiquimod, and the TLR9 Agonist, CpG ODN, Induce Antiviral Cytokines and Chemokines but Do Not Prevent Vaginal Transmission of Simian Immunodeficiency Virus When Applied Intravaginally to Rhesus Macaques. JVI, 16 pgs. PMID: 16254370. (link)

  18. Yang, Jia Jin, et al. (2004). Circumvention of normal constraints on granule protein gene expression in peripheral blood neutrophils and monocytes of patients with antineutrophil cytoplasmic autoantibody-associated glomerulonephritis. JASN, 12 pgs. PMID: 15284296. (link)

  19.  

 

References

 

  1. Staehelin, T., Stähli, C., Hobbs, D.S., and Pestka, S. (1981) "A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies," in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.

  2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) "A Sandwich Radioimmunoassay for Human IFN-a," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.

  3. Human IFN-α international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23.

  4. Rubinstein, M., Levy, W.P., Moschera, J.A., Lai, C.-Y., Hershberg, R.D., Bartlett, R.T., and Pestka, S. (1981) "Human Leukocyte Interferon: Isolation and Characterization of Several Molecular Forms," Arch. Biochem. Biophys. 210, 307-318.

Documentation

Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
41110 Protocol (Full)

41110 Protocol (Full)

41110-1 CoA

41110-1 Certificate of Analysis (CoA)

41110-2 CoA

41110-2 Certificate of Analysis (CoA)

41110 SDS

41110 Safety Data Sheet