Human IFN Alpha Multi-Subtype ELISA Kit (Serum, Plasma, TCM)

Product Features: 
  • Human IFN-Alpha ELISA kit for measuring global human interferon alpha amount in serum, plasma, and tissue culture media (TCM)
  • Greater than 90% spike recovery in normal human serum
  • ELISA assay detects 11 of 12 major human IFN-alpha subtypes
  • Less than 10% inter and intra-assay CV

Full Product Name: VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
41110-1

1 x 96-well plate

$695.00
41110-2

5 x 96-well plate

$2,785.00
$2,975.00

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 12.5 - 1000 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Alpha (No cross-reactivity against human IFN-γ, IFN-β or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; bovine IFN-τ.)
Synonyms: IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
 
Inter-Assay CV: ≤ 8%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 90% in Serum
 
Storage: 2-8°C
Expiration Date: Twenty-one months from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Sample Diluent
  • Antibody Concentrate
  • HRP Conjugate Concentrate
  • Concentrate Diluent
  • TMB Substrate 
  • Stop Solution
  • Dilution Buffer

Tech Info / Data

Image of table of IFN alpha 2a spike recoveries in human serum and plasma using PBL's Human Interferon Alpha Multi-Subtype Serum ELISA kit
Background: 

The VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA kit is designed to provide accurate measurement of global human IFN-alpha amount in serum and plasma samples. This kit quantitates human IFN-alpha in human sera, plasma and tissue culture media using a sandwich immunoassay. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health. This kit can detect 14 different human IFN-alpha subtypes/alleles providing a better view of IFN-alpha production in a sample analysis. 


Interferons (IFNs) are a family of cytokines produced by macrophage, neutrophils, dendritic cells and other somatic cells in response to viruses and pathogens. Interferon Alpha (IFN-alpha), a type I interferon, was one of the first FDA approved biotherapeutic treatments. Additionally, there is a growing amount of evidence suggesting IFN-alpha may be involved in certain autoimmune diseases such as Systemic Lupus Erythematosus (SLE). Accurate and reproducible detection of IFN-alpha in human serum or plasma plays an important role in advancing immunological investigation and drug discovery. 

Citations

Typical Interferon Alpha Standard Curve from 12.5 pg/ml to 1000 pg/ml in Standard Diluent Using PBL's Human IFN Alpha Multi-Subtype Serum ELISA (41110)

 

Image of human IFN alpha standard concentration curve from 12.5 pg/ml to 1000 pg/ml in standard diluent plotted against mean absorbance using PBL's Human IFN Alpha Multi-Subtype Serum ELISA kit

Assay Range: 12.5 - 1000 pg/ml

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