This often-cited ELISA assay quantifies human IFN-Alpha in tissue culture media (TCM) from 12.5-500 pg/ml or from an extended range of 156-5000 pg/ml.
Click here to see which PBL Human IFN-Alpha ELISA kit is best fit for your research need.
Product Name: VeriKine Human Interferon-Alpha ELISA Kit (TCM)
Representative IFN-Alpha Standard Curve from 12.5 pg/ml to 500 pg/ml Using PBL's Human IFN-Alpha ELISA (41100).
Extended Range Interferon Alpha Standard Curve from 156 pg/ml to 5000 pg/ml Using PBL's Human IFN-Alpha ELISA (41100).
| Matrix Compatibility | Tissue Culture Media (TCM) |
|---|---|
| Assay Range | 12.5 - 500 pg/ml or 156 - 5000 pg/ml (Extended Range) |
| Assay Length | 3 hours, 15 minutes |
| Specificity | Human Interferon Alpha |
The VeriKine Human IFN-Alpha ELISA Kit has been developed to quantify levels of human IFN-alpha in cell or tissue culture media (TCM) using a sandwich enzyme-linked immunoassay (ELISA). The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human IFN-Alpha provided by the National Institutes of Health.
*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).
*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).
Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
41100 Protocol (Full)
41100-1 Certificate of Analysis (CoA)
41100-2 Certificate of Analysis (CoA)
41100 Safety Data Sheet
| CVs | Inter-Assay ≤ 8% Intra-Assay ≤ 8% |
|---|---|
| Cross-reactivity | No cross-reactivity against
|
| Synonyms | IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha |
| Storage | 2-8°C |
| Expiration Date | Twenty-one months from the date of manufacture |
| Shipping Condition | Wet Ice |
Tips, Tools and Troubleshooting:
Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.
When IFN interacts with its cognate receptor, a signal is rapidly transmitted within the cell, often producing an antiviral state. The primary signal transduction cascade promoted by type I IFNs is the JAK1-STAT pathway.
Activation of this signal transduction pathway leads to increased gene expression including (2’-5’) oligoadenylate synthetases, Mx proteins, and protein kinase R (PKR) that protect the cell from viral infection.
Powered by Bioz