Human IFN-Alpha ELISA Kit (TCM)
Catalog Number: 41100
This often-cited ELISA assay quantifies human IFN-Alpha in tissue culture media (TCM) from 12.5-500 pg/ml or from an extended range of 156-5000 pg/ml.
|Matrix Compatibility||Tissue Culture Media (TCM)|
|Assay Range||12.5 - 500 pg/ml or 156 - 5000 pg/ml (Extended Range)|
|Assay Length||3 hours, 15 minutes|
|Specificity||Human Interferon Alpha|
The VeriKine Human IFN-Alpha ELISA Kit has been developed to quantify levels of human IFN-alpha in cell or tissue culture media (TCM) using a sandwich enzyme-linked immunoassay (ELISA). The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human IFN-Alpha provided by the National Institutes of Health.
*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).
*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).
Inter-Assay ≤ 8%
Intra-Assay ≤ 8%
No cross-reactivity against
|Synonyms||IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha|
|Expiration Date||Twenty-one months from the date of manufacture|
|Shipping Condition||Wet Ice|
- Pre-coated microtiter plate(s)
- Plate Sealers
- Wash Solution Concentrate
- Human IFN-Alpha Standard, 10,000 pg/ml
- Dilution Buffer
- Antibody Concentrate
- HRP Conjugate Concentrate
- Concentrate Diluent
- TMB Substrate
- Stop Solution
Additional Materials Required (Not Provided)
- Microplate reader capable of reading an OD at a wavelength of 450 nm
- Variable volume microtiter pipettes
- Adjustable multichannel pipette (50-300 μl)
- Reagent reservoirs
- Wash bottle or plate washing system
- Distilled or deionized water
- Serological pipettes (1, 5, 10 or 25 ml)
- Disposable pipette tips (polypropylene)
- Plate shaker
Tech Info & Data
Tips, Tools and Troubleshooting:
Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.
When IFN interacts with its cognate receptor, a signal is rapidly transmitted within the cell, often producing an antiviral state. The primary signal transduction cascade promoted by type I IFNs is the JAK1-STAT pathway.
Activation of this signal transduction pathway leads to increased gene expression including (2’-5’) oligoadenylate synthetases, Mx proteins, and protein kinase R (PKR) that protect the cell from viral infection.
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