Human IFN Alpha ELISA Kit (TCM)

Product Features: 
  • ELISA assay quantifies human IFN alpha in tissue culture media (TCM) down to 12.5 pg/ml
  • Less than 10% inter- and intra-assay CV
  • Less than 4 hours assay time

Full Product Name: VeriKine Human Interferon Alpha ELISA Kit

Ordering

Catalog No. Pack Size Price Quantity
41100-2

5 x 96-well plate

$2,340.00
$2,475.00
41100-1

1 x 96-well plate

$585.00

Specifications

Compatibility: Tissue Culture Media
ELISA Assay Range: 12.5 - 500 pg/ml or 156 - 5000 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Alpha (No cross-reactivity against human IFN-γ, IFN-β or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; bovine IFN-τ.)
Synonyms: IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
 
Inter-Assay CV: ≤ 8%
Intra-Assay CV: ≤ 8%
 
 
Storage: 2-8°C
Expiration Date: Twenty-one months from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Alpha Standard, 10,000 pg/ml
  • Dilution Buffer
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate
  • Stop Solution

Tech Info / Data

Typical human IFN alpha 2a extended range curve from 156 to 5000 pg/ml using PBL Human Interferon Alpha ELISA (41100)
Background: 

The VeriKine Human Interferon Alpha ELISA Kit has been developed to quantify levels of human IFN-alpha in cell or tissue culture media using a sandwich enzyme linked immunoassay (ELISA). The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-α) provided by the National Institutes of Health.


Interferons (IFNs) are a family of mammalian cytokines produced by a variety of cells in response to viral infection, immunity stimulators and chemical inducers. They exhibit a wide range of biological activities that include antiviral, anti-proliferative and immunomodulatory functions. When IFN interacts with its cognate receptor, a signal is rapidly transmitted within the cell, often producing an antiviral state. The primary signal transduction cascade promoted by type I IFNs is the JAK1-STAT pathway. Activation of this signal transduction pathway leads to increased gene expression including (2’-5’) oligoadenylate synthetases, Mx proteins, and protein kinase R (PKR) that protect the cell from viral infection.

Interferon Alpha (IFN-alpha), a type I interferon, was one of the first FDA approved biotherapeutic treatments. Accurate measurement of IFN-alpha in cell or tissue culture media allows evaluation of basic immune responses as well as assessing the potency of Toll-Like Receptor (TLR) agonist-based therapeutics in vitro

Citations

Typical Interferon Alpha Standard Curve from 12.5 pg/ml to 500 pg/ml Using PBL's Human IFN Alpha ELISA (41100)

 

Typical human IFN alpha 2a standard curve from 12.5 to 500 pg/ml using PBL Human Interferon Alpha ELISA kit (41100)

Assay Range: 12.5 - 500 pg/ml

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