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Human IFN-Alpha ELISA Kit (TCM)

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Human IFN-Alpha ELISA Kit (TCM)

Catalog Number: 41100

This often-cited ELISA assay quantifies human IFN-Alpha in tissue culture media (TCM) from 12.5-500 pg/ml or from an extended range of 156-5000 pg/ml.


Product Name: VeriKine Human Interferon-Alpha ELISA Kit (TCM)


Pack Size
Product Info

Matrix Compatibility Tissue Culture Media (TCM)
Assay Range 12.5 - 500 pg/ml or 156 - 5000 pg/ml (Extended Range)
Assay Length 3 hours, 15 minutes
Specificity Human Interferon Alpha


The VeriKine Human IFN-Alpha ELISA Kit has been developed to quantify levels of human IFN-alpha in cell or tissue culture media (TCM) using a sandwich enzyme-linked immunoassay (ELISA). The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human IFN-Alpha provided by the National Institutes of Health.


*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).


*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).


Inter-Assay ≤ 8%
Intra-Assay ≤ 8%

No cross-reactivity against

  • Human IFN-Gamma, IFN-Beta or IFN-Omega
  • Mouse or Rat IFN-Alpha, IFN-Beta or IFN-Gamma
  • Bovine IFN-Tau
Synonyms IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
Storage 2-8°C
Expiration Date Twenty-one months from the date of manufacture
Shipping Condition Wet Ice


Materials Provided

  • Pre-coated microtiter plate(s)
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN-Alpha Standard, 10,000 pg/ml
  • Dilution Buffer
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Concentrate Diluent
  • TMB Substrate
  • Stop Solution


Additional Materials Required (Not Provided) 

  • Microplate reader capable of reading an OD at a wavelength of 450 nm
  • Variable volume microtiter pipettes
  • Adjustable multichannel pipette (50-300 μl)
  • Reagent reservoirs
  • Wash bottle or plate washing system
  • Distilled or deionized water
  • Serological pipettes (1, 5, 10 or 25 ml)
  • Disposable pipette tips (polypropylene)
  • Plate shaker

Tech Info & Data

Tips, Tools and Troubleshooting:




Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory, and many other activities.


When IFN interacts with its cognate receptor, a signal is rapidly transmitted within the cell, often producing an antiviral state. The primary signal transduction cascade promoted by type I IFNs is the JAK1-STAT pathway.


Activation of this signal transduction pathway leads to increased gene expression including (2’-5’) oligoadenylate synthetases, Mx proteins, and protein kinase R (PKR) that protect the cell from viral infection.


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Background Literature:


  1. Staehelin, T., Stähli, C., Hobbs, D.S., and Pestka, S. (1981) “A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies,” in Methods in Enzymology, Vol. 79 (S. Pestka, ed.), Academic Press, New York, 589-595.

  2. Kelder, B., Rashidbaigi, A., and Pestka, S. (1986) “A Sandwich Radioimmunoassay for Human IFNa” in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 582-587.

  3. Human IFN-α international reference standard provided by the NIH, reference no. Gxa01-901-535. Pestka, S. (1986) “Interferon Standards and General Abbreviations,” in Methods in Enzymology, Vol. 119 (S. Pestka, ed.), Academic Press, New York, 14-23.

  4. Rubinstein, M., Levy, W.P., Moschera, J.A., Lai, C.-Y., Hershberg, R.D., Bartlett, R.T., and Pestka, S. (1981) “Human Leukocyte Interferon: Isolation and Characterization of Several Molecular Forms,” Arch. Biochem. Biophys. 210, 307-318.

  5. Hobbs, D.S. and Pestka, (1982) “Purification and Characterization of Interferons from a Continuous Myeloblastic Cell Line,” J. Biol. Chem. 257, 4071-4076.


Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
41100 Protocol (Full)

41100 Protocol (Full)

41100-1 CoA

41100-1 Certificate of Analysis (CoA)

41100-2 CoA

41100-2 Certificate of Analysis (CoA)

41100 SDS

41100 Safety Data Sheet