Simoa Assay Services: Ultra-Sensitive Biomarker Testing

Advantages of Simoa Single Molecule Array Technology:

  • Delivers femtogram per milliliter (fg/ml) level sensitivity for measurement of low-abundance biomarkers in serum, plasma and other complex matrices including bronchial lavage, urine and sputum

  • Provides robust quantitative detection of various single analytes. Comprehensive multiplex panel options also available

  • Offers a high degree of precision, accuracy, and reproducibility with < 10% inter- and intra-assay CVs

  • Over 50 analytes to choose from including IL-17A, TNF-alpha, IL-23, IP-10, TRAIL, IL-6, IL-10 and IL-12p70

For an in-depth overview of the ultra-sensitive biomarker detection technology (Simoa), click here.

 

For additional information, please see the Ultra-Sensitive Biomarker Services Flyer

 

Figure 1. Normal Human Serum (NHS) Endogenous Levels of Several Pro- and Anti-Inflammatory Cytokines

PBL Quanterix Simoa Multiplex shows Normal Human Serum (NHS) Endogenous Levels for IL-1 Beta, IL-6, IL-10 and TNF-Alpha

Interested in our services?

Available Human Simoa Immunoassays:

α-Synuclein
Alpha-Synuclein Assay 

Datasheet

Aβ-40
Amyloid Beta-40 Assay

Datasheet

Aβ-42
Amyloid Beta-42 Assay

Datasheet

BDNF
Brain Derived Neurotrophic Factor Assay

Datasheet

c-MET
c-MET Assay

Datasheet

C-peptide
Connecting Peptide Assay

Datasheet

CA-125
CA-125 Assay

Datasheet

CA-19-9
CA-19-9 Assay

Datasheet

Cathepsin S
Cathepsin S Assay

Datasheet

CEA
Carcino-embryonic Antigen

Datasheet

CRP
C-reactive protein

Datasheet

Cytokine 3-Plex A
TNF-α, IL-6, IL-10

Datasheet

Cytokine 3-Plex B
TNF-α, IL-6, IL-17A

Datasheet

CXCL13
CXC Ligand 13 protein

Datasheet

Eotaxin
Eotaxin Assay

Datasheet

GFAP™
Glial Fibrillary Acidic Protein Assay

Datasheet

G-CSF
Granulocyte Colony Stimulating Factor Assay

Datasheet

GM-CSF
Granulocyte Macrophage Colony Stimulating Factor Assay

Datasheet

HE4/WFDC2
Human epidydimis protein 4

Datasheet

IFN-α
Interferon Alpha Assay

Datasheet

IFN-γ
Interferon Gamma Assay

Datasheet

IL-1α
Interleukin 1 Alpha Assay

Datasheet

IL-1β
Interleukin 1 Beta Assay

Datasheet

IL-2
Interleukin 2 Assay

Datasheet

IL-3
Interleukin 3 Assay

Datasheet

IL-4
Interleukin 4 Assay

Datasheet

IL-5
Interleukin 5 Assay

Datasheet

IL-6
Interleukin 6 Assay

Datasheet

IL-7
Interleukin 7 Assay

Datasheet

IL-8
Interleukin 8 Assay

Datasheet

IL-10
Interleukin 10 Assay

Datasheet

IL-12p40/IL-23
Interleukin 12, p40 Assay

Datasheet

IL-12p70
Interleukin 12, p70 Assay

Datasheet

IL-13
Interleukin 13 Assay

Datasheet

IL-15
Interleukin 15 Assay

Datasheet

IL-17A
Interleukin 17A Assay

Datasheet

IL-17c
Interleukin 17C Assay

Datasheet

IL-18
Interleukin 18 Assay

Datasheet

IL-22
Interleukin 22 Assay

Datasheet

IL-23
Interleukin 23 Assay

Datasheet

IL-28A
Interleukin 28A Assay

Datasheet

IL-33
Interleukin 33 Assay

Datasheet

IL-36β
Interleukin 36 Beta Assay

Datasheet

IP-10
Interferon Gamma Inducible Protein 10 Assay

Datasheet

Leptin
Leptin Assay

 

LIF
Leukemia Inhibitory Factor Assay

Datasheet

MCP-1
Monocyte Chemoattractant Protein-1 Assay

Datasheet

MCP-3
Monocyte Chemoattractant Protein-3 Assay

Datasheet

MIP-1β
Macrophage Inflammatory Protein

Datasheet

MMP-9
Matrix metalloproteinase

Datasheet

Neurology 2-plex A

Datasheet

Neurology 3-plex A Aβ-40, Aβ-42, Tau

Datasheet

Neurology 4-plex A

Datasheet

Neurology 4-plex B

Datasheet

NF-LIGHT®
Neurofilament Light Assay

Datasheet

NSE
Neuron-Specific Enolase Assay

Datasheet

NT-proBNP

Datasheet

p24
Gag Protein p24 Assay

Datasheet

p-Tau181
Phospho-Tau181 Assay

Datasheet

p-Tau231
Phospho-Tau231 Assay

Datasheet

PD-1
Programmed Cell Death Protein 1

Datasheet

PD-L1
Programmed Death Ligand Protein 1

Datasheet

PlGF
Placental Growth Factor Assay

Datasheet

pNF-heavy

Datasheet

PSA
Prostate Specific Antigen Assay

Datasheet

Tau
Total Tau Assay

Datasheet

TDP43
TAR DNA Binding Protein Assay
TGF-α
Transforming Growth Factor Alpha Assay
TGF-β
Transforming Growth Factor Beta Assay
TNF-α
Tumor Necrosis Factor Alpha Assay
TNF-β
Tumor Necrosis Factor Beta Assay
TRAIL
TNF-related Apoptosis-Inducing Ligand Assay
Troponin-I
Cardiac Troponin-I (cTnI) Assay
UCH-L1™
Ubiquitin Carboxyl-Terminal Hydrolase L1 Assay
VEGF
Vascular Endothelial Growth Factor Assay
     

 

 

Available Mouse Simoa Immunoassays:

IL-1α
Interleukin 1 alpha Assay
IL-1β
Interleukin 1 beta Assay
IL-6
Interleukin 6 Assay
IL-17A
Interleukin 17A Assay
IL-17A/F
Interleukin 17A/F Assay
IL-17F
Interleukin 17F Assay
IL-22
Interleukin 22 Assay
IL-23
Interleukin 23 Assay
Tau
Tau Assay
TNF-α
Tumor Necrosis Factor Alpha Assay
   

 

PBL's Ultrasensitive Biomarker Testing Assay Services take advantage of the benefits of Single Molecule Array (Simoa) technology. This approach traps a single bead in low femtoliter-sized wells to allow “digital” measurement of each bead for the presence of analyte in a sandwich immunoassay. The enzymatic amplification of analyte binding vents on single beads in single wells yields low background signals, and can increase assay sensitivities up to several orders of magnitude over conventional assays. Biomarker assays run on this platform frequently exhibit sensitivities in the low to mid fg/ml range.

 

In combination with the markedly heightened sensitivities of these assays, the instrument’s capacity for digital measurement at low concentrations of target analyte and analog measurement at higher analyte concentrations ensures accuracy and precision across a wide dynamic range (> 4 logs) minimizing sample volume requirements and the need for multiple dilutions. Allowing quantification of biomarkers at unprecedented levels in serum, plasma, and additional matrices, PBL’s Simoa-based Assay Services yield robust and reproducible data enabling scientists to attain a better understanding of the contributions of difficult to measure analytes to biological and pathological responses.

 

Powered by SimoaTM (Single Molecule Array) Technology, Quanterix, Lexington, MA, USA.

NF-light® is a registered trademark of Uman Diagnostics, Umeå Sweden.

 

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Technology Summary

Digital measurements are independent of intensity and simply rely on a signal/no signal readout on individual microbeads.

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