Simoa Assay Services: Ultra-Sensitive Biomarker Testing
Advantages of Simoa Single Molecule Array Technology:
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Delivers femtogram per milliliter (fg/ml) level sensitivity for measurement of low-abundance biomarkers in serum, plasma and other complex matrices including bronchial lavage, urine and sputum
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Provides robust quantitative detection of various single analytes. Comprehensive multiplex panel options also available
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Offers a high degree of precision, accuracy, and reproducibility with < 10% inter- and intra-assay CVs
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Over 50 analytes to choose from including IL-17A, TNF-alpha, IL-23, IP-10, TRAIL, IL-6, IL-10 and IL-12p70
For an in-depth overview of the ultra-sensitive biomarker detection technology (Simoa), click here.
For additional information, please see the Ultra-Sensitive Biomarker Services Flyer.
Figure 1. Normal Human Serum (NHS) Endogenous Levels of Several Pro- and Anti-Inflammatory Cytokines
Available Human Simoa Immunoassays:
|
α-Synuclein |
Aβ-40 |
Aβ-42 |
BDNF |
c-MET |
C-peptide |
|
CA-125 |
CA-19-9 |
Cathepsin S |
CEA |
CRP |
Cytokine 3-Plex A |
|
Cytokine 3-Plex B |
CXCL13 |
Eotaxin |
GFAP™ |
G-CSF |
GM-CSF |
|
HE4/WFDC2 |
IFN-α |
IFN-γ |
IL-1α |
IL-1β |
IL-2 |
|
IL-3 |
IL-4 |
IL-5 |
IL-6 |
IL-7 |
IL-8 |
|
IL-10 |
IL-12p40/IL-23 |
IL-12p70 |
IL-13 |
IL-15 |
IL-17A |
|
IL-17c |
IL-18 |
IL-22 |
IL-23 |
IL-28A |
IL-33 |
|
IL-36β |
IP-10 |
Leptin
|
LIF |
MCP-1 |
MCP-3 |
|
MIP-1β |
MMP-9 |
Neurology 2-plex A |
Neurology 3-plex A Aβ-40, Aβ-42, Tau |
Neurology 4-plex A |
Neurology 4-plex B |
|
NF-LIGHT® |
NSE |
NT-proBNP |
p24 |
p-Tau181 |
p-Tau231 |
|
PD-1 |
PD-L1 |
PlGF |
pNF-heavy |
PSA |
Tau |
| TDP43 TAR DNA Binding Protein Assay |
TGF-α Transforming Growth Factor Alpha Assay |
TGF-β Transforming Growth Factor Beta Assay |
TNF-α Tumor Necrosis Factor Alpha Assay |
TNF-β Tumor Necrosis Factor Beta Assay |
TRAIL TNF-related Apoptosis-Inducing Ligand Assay |
| Troponin-I Cardiac Troponin-I (cTnI) Assay |
UCH-L1™ Ubiquitin Carboxyl-Terminal Hydrolase L1 Assay |
VEGF Vascular Endothelial Growth Factor Assay |
Available Mouse Simoa Immunoassays:
| IL-1α Interleukin 1 alpha Assay |
IL-1β Interleukin 1 beta Assay |
IL-6 Interleukin 6 Assay |
IL-17A Interleukin 17A Assay |
IL-17A/F Interleukin 17A/F Assay |
IL-17F Interleukin 17F Assay |
| IL-22 Interleukin 22 Assay |
IL-23 Interleukin 23 Assay |
Tau Tau Assay |
TNF-α Tumor Necrosis Factor Alpha Assay |
PBL's Ultrasensitive Biomarker Testing Assay Services take advantage of the benefits of Single Molecule Array (Simoa) technology. This approach traps a single bead in low femtoliter-sized wells to allow “digital” measurement of each bead for the presence of analyte in a sandwich immunoassay. The enzymatic amplification of analyte binding vents on single beads in single wells yields low background signals, and can increase assay sensitivities up to several orders of magnitude over conventional assays. Biomarker assays run on this platform frequently exhibit sensitivities in the low to mid fg/ml range.
In combination with the markedly heightened sensitivities of these assays, the instrument’s capacity for digital measurement at low concentrations of target analyte and analog measurement at higher analyte concentrations ensures accuracy and precision across a wide dynamic range (> 4 logs) minimizing sample volume requirements and the need for multiple dilutions. Allowing quantification of biomarkers at unprecedented levels in serum, plasma, and additional matrices, PBL’s Simoa-based Assay Services yield robust and reproducible data enabling scientists to attain a better understanding of the contributions of difficult to measure analytes to biological and pathological responses.
Powered by SimoaTM (Single Molecule Array) Technology, Quanterix, Lexington, MA, USA.
NF-light® is a registered trademark of Uman Diagnostics, Umeå Sweden.
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Digital measurements are independent of intensity and simply rely on a signal/no signal readout on individual microbeads.
Find your lot-specific Certificate of Analysis here!
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