Simoa Assay Services: Ultra-Sensitive Biomarker Testing

Home / Simoa Assay Services: Ultra-Sensitive Biomarker Testing

Advantages of Simoa Single Molecule Array Technology:

  • Delivers femtogram per milliliter (fg/ml) level sensitivity for measurement of low-abundance biomarkers in serum, plasma and other complex matrices including bronchial lavage, urine and sputum

  • Provides robust quantitative detection of various single analytes. Comprehensive multiplex panel options also available

  • Offers a high degree of precision, accuracy, and reproducibility with < 10% inter- and intra-assay CVs

  • Over 50 analytes to choose from including IL-17A, TNF-alpha, IL-23, IP-10, TRAIL, IL-6, IL-10 and IL-12p70

For an in-depth overview of the ultra-sensitive biomarker detection technology (Simoa), click here.

 

For additional information, please see the Ultra-Sensitive Biomarker Services Flyer. 

 

Figure 1. Normal Human Serum (NHS) Endogenous Levels of Several Pro- and Anti-Inflammatory Cytokines

PBL Quanterix Simoa Multiplex shows Normal Human Serum (NHS) Endogenous Levels for IL-1 Beta, IL-6, IL-10 and TNF-Alpha

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Available Quanterix Simoa Immunoassays on HD-X Instrument:

*The Representative Functional LLOQ is the assay’s Representative Analytical LLOQ corrected for a Recommended Sample Dilution Factor specific to each assay. Both the Representative Calibration Range and Functional LLOQ are often lot-specific.

Assay [Catalog No.]

 Species Matrix Representative Calibration Range Representative Functional LLOQ Datasheet
α-Synuclein [102233]
Alpha Synuclein Discovery Assay Kit		 Human

CSF, Plasma, Serum

4.12 – 1,000 pg/mL

 41.2 pg/mL Datasheet
Aβ-40 [101672]
Amyloid Beta 40 Advantage Assay Kit		 Human

CSF, Plasma

 2.47 – 200 pg/mL

CSF: 49.2 pg/mL

P: 4.92 pg/mL

 Datasheet
Aβ-42 [101664]
Amyloid Beta 42 Advantage Assay Kit		 Human CSF, Plasma 0.137 – 100 pg/mL

CSF: 1.37 pg/mL

P: 0.548 pg/mL

 Datasheet

BDNF [102039]
Brain Derived Neurotrophic Factor Discovery Assay Kit

 Human

CSF, Plasma, Serum

 0.029 – 120 pg/mL

CSF: 0.059 pg/mL

P: 14.65 pg/mL

S: 14.65 pg/mL

 Datasheet
c-MET [102073]
c-MET Discovery Assay Kit		 Human

Cell Lysates, Plasma, Serum

 0.488 – 2,000 pg/mL

97.6 pg/mL

 Datasheet
C-peptide [100199]
Connecting Peptide Advantage Assay Kit		 Human

Plasma, Serum

 0.137 – 100 pg/mL

T1 Diabetes: 0.084 pg/mL

T2 Diabetes: 0.21 pg/mL

 Datasheet
CA-125 [102136]
Cancer Antigen 125 Discovery Assay Kit		 Human Plasma, Serum 0.027 – 20 U/mL Not Given  
CA-19-9 [102543]
Cancer Antigen 19-9 Discovery Assay Kit		 Human Plasma, Serum 0.165 – 120 U/mL 0.082 U/mL Datasheet
Cathepsin S [102064]
Cathepsin S Discovery Assay Kit		 Human Plasma, Serum 1.95 – 2,000 pg/mL 195 pg/mL  Datasheet
CEA [102556]
Carcinoembryonic Antigen Discovery Assay Kit		 Human Plasma, Serum 2.33 – 1,700 pg/mL 116.5 pg/mL Datasheet
CRP [102583]
C-reactive Protein Discovery Assay Kit		 Human Plasma, Serum 1.37 – 1,000 pg/mL 32.93 pg/mL Datasheet
cTnI [101588]
Cardiac Troponin I Advantage Assay Kit		 Human Plasma, Serum 0.098 – 300 pg/mL 0.316 pg/mL Datasheet
CXCL13 [102635]
CXC Ligand 13 Protein Discovery Assay Kit		 Human Plasma, Serum

0.274 – 200 pg/mL

0.28 pg/mL

 Datasheet
C3PA [101160]
Cytokine 3-Plex A Advantage Assay Kit (for IL-6, IL-10, TNF-α)		 Human Plasma, Serum

IL-6: 0.043 – 15 pg/mL

IL-10: 0.023 – 6 pg/mL

TNF-α: 0.088 – 28 pg/mL

IL-6: 0.044 pg/mL

IL-10: 0.292 pg/mL

TNF-α: 0.204 pg/mL

 Datasheet
C3PB [101319]
Cytokine 3-Plex B Advantage Assay Kit (for IL-6, IL-17A, TNF-α)		 Human Plasma, Serum

IL-6: 0.048 – 15 pg/mL

IL-17A: 0.05 –10 pg/mL

TNF-α: 0.097 – 28 pg/mL

IL-6: 0.092 pg/mL

IL-17A: 0.028 pg/mL

TNF-α: 0.104 pg/mL

 Datasheet
Eotaxin [101212]
Eotaxin Advantage Assay Kit		 Human  Plasma, Serum 0.165 – 120 pg/mL 0.72 pg/mL Datasheet
G-CSF [101235]
Granulocyte Colony Stimulating Factor Advantage Assay Kit		 Human Plasma, Serum 0.274 – 200 pg/mL 0.38 pg/mL Datasheet
GFAP™ [102336]
Glial Fibrillary Acidic Protein Discovery Assay Kit		 Human CSF, Plasma, Serum 1.37 – 1,000 pg/mL

CSF: 27.44 pg/mL

P: 2.744 pg/mL

S: 2.744 pg/mL

 

 Datasheet
GM-CSF [102329]
Granulocyte Macrophage Colony-Stimulating Factor Advantage Assay Kit		 Human Plasma, Serum 0.041 – 30 pg/mL 0.041 pg/mL Datasheet
HE-4/WFDC2 [103059]
Human Epididymis Protein 4 Discovery Assay Kit		 Human Plasma, Serum 0.977 – 1,000 pg/mL 3.908 pg/mL Datasheet
HIV p24 [102215]
Human Immunodeficiency Virus Gag Protein 24 Advantage Assay Kit		 Human Plasma, Serum 0.01 – 30 pg/mL 0.02 pg/mL Datasheet
IFN-α [100860]
Interferon Alpha Advantage Assay Kit		 Human Serum 0.97 – 30 pg/mL 0.009 pg/mL Datasheet
IFN-γ [103337]
Interferon Gamma Advantage Assay Kit		 Human Plasma, Serum 0.049 – 50 pg/mL 0.196 pg/mL Datasheet
IL-1α [101968]
Interleukin 1 Alpha Discovery Assay Kit		 Human Plasma, Serum 0.041 – 30 pg/mL 0.02 pg/mL Datasheet
IL-1α [102504]
Interleukin 1 Alpha Discovery Assay Kit		 Mouse Plasma, Serum 0.096 – 70 pg/mL 0.6 pg/mL Datasheet
IL-1β [101605]
Interleukin 1 Beta Advantage Assay Kit		 Human Plasma, Serum 0.165 – 120 pg/mL 0.166 pg/mL Datasheet
IL-1β [102109]
Interleukin 1 Beta Discovery Assay Kit		 Mouse Plasma, Serum 1.01 – 160 pg/mL 0.48 pg/mL Datasheet
IL-2 [101635]
Interleukin 2 Advantage Assay Kit		 Human Plasma, Serum 0.123 – 30 pg/mL 0.164 pg/mL Datasheet
IL-3 [102462]
Interleukin 3 Discovery Assay Kit		 Human Plasma, Serum 0.686 – 500 pg/mL 2.744 pg/mL Datasheet
IL-4 [100196]
Interleukin 4 Advantage Assay Kit		 Human Plasma, Serum 0.137 – 100 pg/mL 0.078 pg/mL Datasheet
IL-5 [102860]
Interleukin 5 Advantage Assay Kit		 Human Plasma, Serum 0.016 – 12 pg/mL 0.033 pg/mL Datasheet
IL-6 [101622]
Interleukin 6 Advantage Assay Kit		 Human Plasma, Serum 0.041 – 30 pg/mL 0.04 pg/mL Datasheet
IL-6 [102109]
Interleukin 6 Discovery Assay Kit		 Mouse Plasma, Serum 1.012 – 160 pg/mL 0.48 pg/mL Datasheet
IL-7 [103277]
Interleukin 7 Advantage Assay Kit		 Human Plasma, Serum 0.206 – 150 pg/mL 0.412 pg/mL Datasheet

IL-8 [100198]
Interleukin 8 Advantage Assay Kit

 Human Plasma, Serum 0.073 – 300 pg/mL 0.368 pg/mL Datasheet
IL-10 [101643]
Interleukin 10 Advantage Assay Kit		 Human Plasma, Serum 0.041 – 30 pg/mL 0.084 pg/mL Datasheet
IL-12p40/IL-23 [101871]
Interleukin 8 Advantage Assay Kit		 Human Plasma, Serum 0.343 – 250 pg/mL 0.344 pg/mL Datasheet
IL-12p70 [100988]
Interleukin 12p70 Advantage Assay Kit		 Human Plasma, Serum 0.014 – 10 pg/mL 0.068 pg/mL Datasheet
IL-13 [102732]
Interleukin 13 Advantage Assay Kit		 Human Plasma, Serum 0.004 – 15 pg/mL 0.01 pg/mL Datasheet
IL-15 [100794]
Interleukin 15 Advantage Assay Kit		 Human Plasma, Serum 0.014 – 10 pg/mL 0.0248 pg/mL Datasheet
IL-17A [101599]
Interleukin 17A Advantage Assay Kit		 Human Plasma, Serum 0.041 – 30 pg/mL 0.084 pg/mL Datasheet
IL-17A [101824]
Interleukin 17A Advantage Assay Kit		 Mouse Plasma, Serum 0.97 – 300 pg/mL 1.648 pg/mL Datasheet
IL-17C [102570]
Interleukin 17C Discovery Assay Kit		 Human Plasma, Serum 0.412 – 300 pg/mL 0.824 pg/mL Datasheet
IL-17F [101839]
Interleukin 17F Discovery Assay Kit		 Mouse Plasma, Serum 1.01 – 600 pg/mL 1.648 pg/mL Datasheet
IL-18 [102700]
Interleukin 18 Discovery Assay Kit		 Human Plasma, Serum 0.011 – 45 pg/mL 0.6 pg/mL Datasheet
IL-22 [103071]
Total Interleukin 22 Discovery Assay Kit		 Human Plasma, Serum 0.041 – 30 pg/mL 0.041 pg/mL Datasheet
IL-22 [101919]
Interleukin 22 Discovery Assay Kit		 Mouse Plasma, Serum 0.982 – 300 pg/mL 0.824 pg/mL Datasheet
IL-23 [101847]
Interleukin 23 Discovery Assay Kit		 Mouse Plasma, Serum 0.964 – 200 pg/mL 0.548 pg/mL Datasheet
IL-28A [101419]
Interleukin 28A Discovery Assay Kit		 Human Plasma, Serum 0.137 – 100 pg/mL 0.138 pg/mL Datasheet
IL-33 [103093]
Interleukin 33 Discovery Assay Kit		 Human Plasma, Serum 1.372 – 1,000 pg/mL 1.372 pg/mL Datasheet
IL-36β [101808]
Interleukin 36 Beta Discovery Assay Kit		 Human Plasma, Serum 0.206 – 150 pg/mL 0.824 pg/mL Datasheet

IP-10 [101132]
Interferon Gamma-Inducible Protein 10 Advantage Assay Kit

 Human Plasma, Serum 0.274 – 200 pg/mL 0.708 pg/mL Datasheet
LIF [102394]
Leukemia Inhibitory Factor Discovery Assay Kit

Human

 Plasma, Serum 0.981 – 120 pg/mL 0.344 pg/mL Datasheet
MCP-1 [101154]
Monocyte Chemoattractant Protein 1 Advantage Assay Kit		 Human Plasma, Serum 0.823 – 200 pg/mL 0.612 pg/mL Datasheet
MCP-3 [102382]
Monocyte Chemoattractant Protein 3 Discovery Assay Kit		 Human Plasma, Serum 0.309 – 225 pg/mL 0.618 pg/mL Datasheet
MMP-9 [102491]
Matrix Metalloproteinase 9 Discovery Assay Kit		 Human CSF, Platelet-Poor Heparin Plasma, Serum 4.88 – 5,000 pg/mL

CSF: 39.04 pg/mL

P: 4,880 pg/mL

S: 4,880 pg/mL

 

 

 Datasheet
N2PB [103520]
Neurology 2-Plex B Advantage Assay Kit (for GFAP, NF-Light)		 Human CSF, Plasma, Serum

GFAP: 10 – 9,000 pg/mL

NF-L: 0.5 – 450 pg/mL

(in Plasma)

GFAP: 16.6 pg/mL

NF-L: 0.8 pg/mL

 Datasheet
N3PA [101995]
Neurology 3-Plex A Advantage Assay Kit (for Aβ-40, Aβ-42, Tau)		 Human CSF, Plasma, Serum

Aβ-40: 0.777 – 130 pg/mL

Aβ-42: 0.18 – 64 pg/mL

Tau: 0.1 – 100 pg/mL

(in Plasma)

Aβ-40: 2.7 pg/mL

Aβ-42: 0.568 pg/mL

Tau: 0.252 pg/mL

 Datasheet
N4PA [102153]
Neurology 4-Plex A Advantage Assay Kit (for GFAP, NF-Light, Tau, UCH-L1)		 Human CSF, Plasma, Serum

GFAP: 1.02 – 1,000 pg/mL

NF-L: 0.554 – 500 pg/mL

Tau: 0.123 – 100 pg/mL

UCH-L1: 13.8 – 10,000 pg/mL

(in Plasma)

GFAP: 1.868 pg/mL

NF-L: 0.964 pg/mL

Tau: 0.5 pg/mL

UCH-L1: 37.52 pg/mL

Datasheet
N4PB [103345]
Neurology 4-Plex B Advantage Assay Kit (for GFAP, NF-Light, Tau, UCH-L1)		 Human CSF, Plasma, Serum

GFAP: 10 – 9,000 pg/mL

NF-L: 0.50 – 450 pg/mL

Tau: 0.1 – 90 pg/mL

UCH-L1: 10 – 9,000 pg/mL

(in Plasma)

GFAP: 37.52 pg/mL

NF-L: 2 pg/mL

Tau: 0.5 pg/mL

UCH-L1: 37.52 pg/mL

 Datasheet

NF-LIGHT® [103186]
Neurofilament-Light Advantage Assay Kit

 Human CSF, EDTA Plasma, Heparin Plasma, Serum 0.538 – 500 pg/mL

CSF: 17.4 pg/mL

P: 0.696 pg/mL

S: 0.696 pg/mL

 Datasheet

NSE [102475]
Neuron-Specific Enolase Discovery Assay Kit

 

 Human Plasma, Serum 9.88 – 2,400 pg/mL 494 pg/mL Datasheet
NT-proBNP [102713]
N-Terminal Pro B Type Natriuretic Peptide Discovery Assay Kit		 Human Plasma, Serum 0.206 – 50 pg/mL 2.06 pg/mL Datasheet
PD-1 [102929]
Programmed Cell Death Protein 1 Discovery Assay Kit		 Human Plasma, Serum 1.76 – 1,800 pg/mL 3.516 pg/mL Datasheet
PD-L1 [102648]
Programmed Death Ligand 1 Discovery Assay Kit		 Human Plasma, Serum 0.885 – 215 pg/mL 2.1 pg/mL Datasheet
PLGF [102318]
Placental Growth Factor Discovery Assay Kit		 Human Plasma, Serum 0.329 – 240 pg/mL 1.2 pg/mL Datasheet
pNF-Heavy [102669]
Phosphorylated Neurofilament-Heavy Discovery Assay Kit		 Human

(Human) CSF, Plasma, Serum

(Mouse) Plasma, Serum

 2.88 – 2,100 pg/mL

(H)CSF: 72 pg/mL

(H)P: 11.52 pg/mL

(H)S: 11.52 pg/mL

(M)P: 28.8 pg/mL

(M)S: 28.8 pg/mL

 Datasheet

PSA [101478]
Prostate Specific Antigen Advantage Assay Kit

 Human Plasma, Serum 0.1 – 100 pg/mL 0.096 pg/mL Datasheet
p-Tau 181 [103714]
Tau Phosphorylated at Threonine 181 Advantage Assay Kit		 Human CSF, Plasma, Serum 0.169 – 103 pg/mL

CSF: 0.67 pg/mL

P: 0.268 pg/mL

S: 0.268 pg/mL

 Datasheet
p-Tau 231 [102292]
Tau Phosphorylated at Threonine 231 Advantage Assay Kit		 Human CSF 0.41 – 300 pg/mL 7.32 pg/mL Datasheet
Tau [101522]
Tau Advantage Assay Kit		 Human CSF, Plasma, Serum 0.054 – 100 pg/mL

CSF: 0.61 pg/mL

P: 0.244 pg/mL

S: 0.244 pg/mL

 Datasheet
Tau [102209]
Tau Discovery Assay Kit		 Mouse CSF, Plasma, Serum 0.823 – 600 pg/mL

CSF: 8.23 pg/mL

P: 3.292 pg/mL

S: 3.292 pg/mL

 Datasheet

TDP-43 [103293]
TAR DNA Binding Protein (43 kDa) Advantage Assay Kit

 Human CSF, Plasma, Serum 8.23 – 2,000 pg/mL 32.92 pg/mL Datasheet
TGF-α [101863]
Transforming Growth Factor Alpha Discovery Assay Kit		 Human Plasma, Serum 0.617 – 450 pg/mL 0.414 pg/mL Datasheet
TGF-β [101984]
Transforming Growth Factor Beta Discovery Assay Kit		 Human Plasma, Serum 2.06 – 1,500 pg/mL 8.224 pg/mL Datasheet

TNF-α [101580]
Tumor Necrosis Factor Alpha Advantage Assay Kit

 Human Plasma, Serum 0.069 – 50 pg/mL 0.136 pg/mL Datasheet

TNF-α [102100]
Tumor Necrosis Factor Alpha Discovery Assay Kit

 Mouse Plasma, Serum 2.47 – 1,800 pg/mL 4.92 pg/mL Datasheet
TNF-β [102091]
Tumor Necrosis Factor Beta Discovery Assay Kit		 Human Plasma, Serum 0.95 – 600 pg/mL 0.6 pg/mL Datasheet
TRAIL [100906]
Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Advantage Assay Kit		 Human Plasma, Serum 0.137 – 100 pg/mL 0.072 pg/mL Datasheet
UCH-L1™ [102343]
Ubiquitin C-Terminal Hydrolase L1 Discovery Assay Kit		 Human CSF, Plasma, Serum 6.86 – 5,000 pg/mL

CSF: 34.3 pg/mL

P: 13.72 pg/mL

S: 13.72 pg/mL

 Datasheet
VEGF [102794]
Vascular Endothelial Growth Factor Discovery Assay Kit		 Human Plasma, Serum 0.274 – 200 pg/mL 0.548 pg/mL Datasheet

 

 

 

 

 

 

 

 

 

 

 

 

 

PBL's Ultrasensitive Biomarker Testing Assay Services take advantage of the benefits of Single Molecule Array (Simoa) technology. This approach traps a single bead in low femtoliter-sized wells to allow “digital” measurement of each bead for the presence of analyte in a sandwich immunoassay. The enzymatic amplification of analyte binding vents on single beads in single wells yields low background signals, and can increase assay sensitivities up to several orders of magnitude over conventional assays. Biomarker assays run on this platform frequently exhibit sensitivities in the low to mid fg/ml range.

 

In combination with the markedly heightened sensitivities of these assays, the instrument’s capacity for digital measurement at low concentrations of target analyte and analog measurement at higher analyte concentrations ensures accuracy and precision across a wide dynamic range (> 4 logs) minimizing sample volume requirements and the need for multiple dilutions. Allowing quantification of biomarkers at unprecedented levels in serum, plasma, and additional matrices, PBL’s Simoa-based Assay Services yield robust and reproducible data enabling scientists to attain a better understanding of the contributions of difficult to measure analytes to biological and pathological responses.

 

Powered by SimoaTM (Single Molecule Array) Technology, Quanterix, Lexington, MA, USA.

NF-light® is a registered trademark of Uman Diagnostics, Umeå Sweden.

 

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Technology Summary

Digital measurements are independent of intensity and simply rely on a signal/no signal readout on individual microbeads.

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